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Journal : JURNAL ATOMIK

SCREENING LIPASE FROM ENDOPHYTIC BACTERIA FROM STEAM OF (Costus speciosus (J.Koenig) Sm) AND OPTIMUM WORKING CONDITION Asjayani Kurnia Sari; Winni Astuti; Djihan Ryn Pratiwi
JURNAL ATOMIK Vol 5 No 1 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Endophytic bacteria are bacteria that live in plant tissues such as the roots, leaves, and stems of plants. This research was conducted to screen lipase-producing endophytic bacteria from Pacing stem (Costus speciosus (J.Koenig) Sm.) and determine the optimum working conditions. Screening of lipase-producing bacteria on nutrient agar media containing olive oil and Rhodamine B showed that one isolate was able to produce lipase. The crude lipase extract produced from endophytic bacteria worked optimally at a pH of 7, the temperature of 40℃, and a substrate concentration of 2%. Keywords : Costus speciosus (J.Koenig) Sm., Endophytic bacteria, Lipase, Optimum Activity
SCREENING BAKTERI PENGHASIL AMILASE DARI SEDIMEN SUMBER AIR PANAS DONDANG MUARA JAWA M Nur Yasin Fahrul Fhozi; Winni Astuti; Rahmat Gunawan
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The screening of amylase enzyme producing thermophilic bacteria from sediment of hot spring in Muara Jawa has been done. Screening has done by using selective media of starch, will show the formation of clear zones around the colonies by dripping the iodine solution. From alot colonies selected 1 colony with widest clear zone. Single colony obtained was identified as Bacillus sp. Amylase produced was tested for amylase activity by DNS method and obtained the amylase activity at pH 6 and temperature at 55°C is 2,8460 U/mL.
SCREENING BACTERIAL OF ENDOPHYTIC PRODUCING AMYLASE, LIPASE AND PROTEASE FROM LEAVES OF Macaranga hullettii King ex Hook.f. Sherly Pricilia; Winni Astuti; Eva Marliana
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The objective of this study was to screening test of endophytic bacteria producing amylase, lipase and protease from leaves Macaranga hullettii King ex Hook.f. Endophytic bacteria isolation was performed on nutrient agar (NA) and 21 single colonies of endophytic bacteria were obtained. Endophytic bacteria that all of the endophytic bacteria positive producing enzym, 8 of the endophytic bacteria positive amylase, 7 of the endophytic bacteria positive lipase and 4 of the endophytic bacteria positive protease.