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All Journal Jurnal Kimia Mulawarman JURNAL ATOMIK al Kimiya : Jurnal Ilmu Kimia dan Terapan
Winni Astuti
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Mulawarman University
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

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Isolasi Dan Karakterisasi Lipase Dari Rebung Bambu Betung Dendrocalamusasper Agung Prasetyo; Winni Astuti; Chairul Saleh
JURNAL KIMIA MULAWARMAN Vol 15 No 1 (2017)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v15i1.585

Abstract

This research was conducted to get the extract of rough lipase produced by bamboo shoots (Dendrocalamus asper). Lipase isolation from bamboo shoots was done by blending bamboo shoots to produce the crude extract of the enzyme. Lipase crude extracts that occurred at centrifugation at 12000 rpm for 30 minutes at 4°C. The crude extracts obtained were then tested using quantitative pH 7, temperature 30ºC and substrate 1% (v/v). Based on research there is activity of crude extract of lipase equal to 2,24 U/ml.
PENENTUAN KADAR ASAM AMINO ESENSIAL (METIONIN, LEUSIN, ISOLEUSIN DAN LISIN) PADA TELUR PENYU DAN TELUR BEBEK agita rachmala ginting; saibun sitorus; winni astuti
JURNAL KIMIA MULAWARMAN Vol 14 No 2 (2017)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Determination of amino acids essential’s content (methionine, leucine, isoleucine and lysine) on turtle eggs and duck eggs. Turtle eggs and duck eggs are a high animal protein source and easy to obtain. This research has been carried out by categorizing of turtle eggs and duck eggs by size and continued analysis of water content by Methods of Gravimetry, analyzes protein content total by the method of Kjeldahl and analysis of the levels of essential amino acids with method of HPLC (High Performance Liquid Chromatography). In this study, the researcher found the water content in the mix (large, medium and small) 75.2889% and turtle eggs on the mix 67.9020% duck egg. Total protein content in the mix turtle eggs 8.9268% and the mix duck eggs 14.2455%. Levels of essential amino acids in the mixture turtle eggs undetectable methionine, 3.25% leucine, isoleucine and lysine 1.53% 2.50%. In the mix duck eggs undetectable methionine, 1.30% leucine, isoleucine and lysine 0.58% to 0.38%.
SCREENING LIPASE FROM ENDOPHYTIC BACTERIA FROM STEAM OF (Costus speciosus (J.Koenig) Sm) AND OPTIMUM WORKING CONDITION Asjayani Kurnia Sari; Winni Astuti; Djihan Ryn Pratiwi
JURNAL ATOMIK Vol 5 No 1 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Endophytic bacteria are bacteria that live in plant tissues such as the roots, leaves, and stems of plants. This research was conducted to screen lipase-producing endophytic bacteria from Pacing stem (Costus speciosus (J.Koenig) Sm.) and determine the optimum working conditions. Screening of lipase-producing bacteria on nutrient agar media containing olive oil and Rhodamine B showed that one isolate was able to produce lipase. The crude lipase extract produced from endophytic bacteria worked optimally at a pH of 7, the temperature of 40℃, and a substrate concentration of 2%. Keywords : Costus speciosus (J.Koenig) Sm., Endophytic bacteria, Lipase, Optimum Activity
SCREENING BAKTERI PENGHASIL AMILASE DARI SEDIMEN SUMBER AIR PANAS DONDANG MUARA JAWA M Nur Yasin Fahrul Fhozi; Winni Astuti; Rahmat Gunawan
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The screening of amylase enzyme producing thermophilic bacteria from sediment of hot spring in Muara Jawa has been done. Screening has done by using selective media of starch, will show the formation of clear zones around the colonies by dripping the iodine solution. From alot colonies selected 1 colony with widest clear zone. Single colony obtained was identified as Bacillus sp. Amylase produced was tested for amylase activity by DNS method and obtained the amylase activity at pH 6 and temperature at 55°C is 2,8460 U/mL.
SCREENING BACTERIAL OF ENDOPHYTIC PRODUCING AMYLASE, LIPASE AND PROTEASE FROM LEAVES OF Macaranga hullettii King ex Hook.f. Sherly Pricilia; Winni Astuti; Eva Marliana
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The objective of this study was to screening test of endophytic bacteria producing amylase, lipase and protease from leaves Macaranga hullettii King ex Hook.f. Endophytic bacteria isolation was performed on nutrient agar (NA) and 21 single colonies of endophytic bacteria were obtained. Endophytic bacteria that all of the endophytic bacteria positive producing enzym, 8 of the endophytic bacteria positive amylase, 7 of the endophytic bacteria positive lipase and 4 of the endophytic bacteria positive protease.
Potensi Bakteri Endofit Daun Pucuk Merah (Syzygium Myrtifolium Walp.) Sebagai Antibakteri Terhadap Propionibacterium Acnes Wihda Nisa Alhayyu; Winni Astuti; Eva Marliana
JURNAL KIMIA MULAWARMAN Vol 20 No 1 (2022)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v20i1.1015

Abstract

Salah satu penyebab timbulnya jerawat yaitu adanya infeksi bakteri Propionibacterium acnes. Infeksi bakteri penyabab jerawat dapat diatasi menggunakan antibakteri. Pemanfaatan bakteri endofit adalah cara lain untuk mendapatkan metabolit sekunder selain menggunakan ekstrak tanaman. Bakteri endofit adalah bakteri yang hidup di dalam jaringan tanaman dan menghasilkan senyawa bioaktif yang sama dengan tanaman inangnya. Penelitian ini bertujuan untuk mengisolasi bakteri endofit daun hijau dari tanaman pucuk merah dan memanfaatkan senyawa metabolit sekundernya sebagai antibakteri. Pada penelitian ini dilakukan uji aktivitas antibakteri menggunakan metode difusi sumuran terhadap bakteri Propionibacterium acnes KCCM 41747. Hasil penelitian diperoleh sebanyak 30 isolat bakteri endofit hasil isolasi berpotensi sebagai antibakteri terhadap Propionibacterium acnes. Aktivitas antibakteri tertinggi terdapat pada kode koloni P27 dengan diameter zona bening sebesar 23,17 mm. Berdasarkan hasil uji aktivitas antibakteri dapat disimpulkan bahwa bakteri endofit daun hijau dari tanaman pucuk merah berpotensi sebagai antibakteri terhadap bakteri Propionibacterium acnes KCCM 41747.
Pembuatan Edible Film Yang Bersifat Antibakteri Dari Glukomanan Umbi Porang (Amorphophallus Muelleri) Yang Diinkorporasi Dengan Ekstrak Etanol Umbi Bawang Tiwai (Eleutherine Bulbosa (Mill.) Urb.) Anggun Ridha Avitri; Subur P Pasaribu; Winni Astuti
JURNAL KIMIA MULAWARMAN Vol 20 No 1 (2022)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v20i1.1073

Abstract

ABSTRAK Penelitian mengenai pembuatan edible film yang bersifat antibakteri dari glukomanan umbi Porang (Amorphophallus muelleri) yang diinkorporasi dengan ekstrak etanol umbi Bawang Tiwai (Eleutherine bulbosa (Mill.) Urb.) untuk mendapatkan formula edible film glukomanan umbi Porang dan ekstrak etanol umbi Bawang Tiwai yang memiliki aktivitas antibakteri terhadap bakteri Escherichia coli ATCC 25922 dan Staphylococcus aureus ATCC 25923 telah selesai dilakukan. Aktivitas antibakteri ekstrak etanol umbi Bawang Tiwai dilakukan dengan metode difusi agar dengan konsentrasi 2, 4, 6, dan 8% menunjukkan bahwa ekstrak etanol umbi Bawang Tiwai dengan konsentrasi 4% memiliki aktivitas antibakteri terhadap kedua bakteri masing-masing sebesar 13 mm dan 15,83 mm. Formula edible film yang mengandung glukomanan 6%, gliserol 25%, dan ekstrak etanol umbi Bawang Tiwai 4% menunjukkan aktivitas antibakteri terhadap bakteri Escherichia coli ATCC 25922 dan Staphylococcus aureus ATCC 25923 masing-masing sebesar 14,83 mm dan 16 mm.
RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA Astuti, Winni; Adawiyah, Rabiatul; Putri, Amanda Aulia; Pratiwi, Djihan Ryn
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 11, No 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.39438

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.
RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA Astuti, Winni; Adawiyah, Rabiatul; Putri, Amanda Aulia; Pratiwi, Djihan Ryn
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol. 11 No. 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.39438

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.
Co-Authors agita rachmala ginting Agung Prasetyo Anggun Ridha Avitri Asjayani Kurnia Sari Chairul Saleh Djihan Ryn Pratiwi Djihan Ryn Pratiwi, Djihan Ryn Eva Marliana Eva Marliana M Nur Yasin Fahrul Fhozi Prasetyo, Agung Putri, Amanda Aulia Rabiatul Adawiyah Rahmat Gunawan Saibun Sitorus Saleh, Chairul Sherly Pricilia Subur P Pasaribu Wihda Nisa Alhayyu