Eka Fatmawati Tihurua
Botany Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor Km 46 Cibinong 16911

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KERAGAMAN KERAPATAN KAYU BATANG DAN CABANG KOMUNITAS POHON DI HUTAN GUNUNG PAPANDAYAN, JAWA BARAT Tihurua, Eka Fatmawati; Sulistyawati, Endah
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3519

Abstract

Wood density is the functional character which has important role in the function of ecosystem. Stem and branch wood density have different trait and effect on its ecosystem processes. The objectives of this research are to know the diversity of stem and branch wood density and to analyze whether branch wood density could be used to estimate stem wood density in the Mount Papandayan. Six plots of 0.1 ha at different sites (three plots each in interior and edge forest) were established. Branches were collected from trees with diameter at breast height larger than 10 cm, while stem wood density data were obtained from some wood density sources. Research results showed that stem wood density ranges were 0.35?0.82 g/cm3, while branch wood density ranges were 0.33?0.61 g/cm3. Average of branch wood density was lower (0.48 ± 0.09 g/cm3) than stem wood density (0.61 ± 0.1 g/cm3). Linear regression analysis indicated that branch wood density could be used to estimate stem wood density of trees in Mount Papandayan which is showed by R2 value and correlation coefficient of 0.28 and 0.55 (p value < 0.001) respectively. 
LEAF ANATOMICAL ADAPTATION OF EXOTIC INVASIVE MICONIA CRENATA (VAHL.) MICHELANG ALONG ALTITUDINAL GRADIENT: A CASE STUDY OF MOUNT GEDE PANGRANGO NATIONAL PARK, WEST JAVA, INDONESIA JUNAEDI, DECKY INDRAWAN; TIHURUA, EKA FATMAWATI; WIDOYANTI, WIDOYANTI; GIRMANSYAH, DEDEN
REINWARDTIA Vol 23, No 1 (2024): Volume 23, No 1 (2024): Reinwardtia
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4755

Abstract

Miconia crenata is a widely spread species that occurs in multiple ecosystems. However, there is limited information on M. crenata invasion biology, ecology, and anatomy, particularly in the context of mountainous tropical forest and biodiversity management. Therefore, we examined elevation effects upon leaf anatomical structure of exotic invasive M. crenata at Mount Gede-Pangrango National Park (MGPNP). We sampled the leaves at four different elevations i.e., 715 m asl, 800 m asl, 900 m asl, and 1,000 m asl. Cross section leaf anatomy specimens of M. crenata were obtained by using paraffin method and stained with safranin and fast green. This study found that M. crenata leaf anatomy was correlated with elevation shown by changes in leaf tissue thickness and stomata size. Further study is needed of leaf anatomical variation of exotic invasive species along driven by soil properties and the variation across different plant taxa and growth forms. Such studies are important to determine adaptation capacity of invasiveness.
Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Tihurua, Eka Fatmawati; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.307-319

Abstract

Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis, from induction, culture proliferation, and somatic embryo development to plantlet conversion. The initial stages of embryogenesis, characterized by nodules, were primarily composed of meristematic cells with high cell division activity. These cells contained tetrad-like structures that could develop into distinct two- and four-celled proembryoids or proembryogenic aggregates. Our histo-anatomical analysis revealed that embryogenic cultures proliferated through multiple pathways simultaneously: somatic embryo budding, proembryo formation, and pro-embryonic mass formation from both internal and peripheral cells. At the stage of somatic embryo development, embryos with a well-defined protoderm layer, containing cells with prominent nuclei and dense cytoplasm, potentially regenerate into plantlets. Furthermore, histological examination revealed the presence of procambium within mature somatic embryos, which subsequently developed into the vascular system of the complete plantlet
The changes of chili leaf structure by Geminivirus infection Taufik, Muhammad; Firihu, Muhammad Zamrun; HS, Gusnawaty; Variani, Viska Inda; Hasan, Asmar; Botek, Muhammad; Tihurua, Eka Fatmawati; Wulansari, Tri Yuni Indah
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.124109-119

Abstract

The Geminivirus in chili plants is a significant problem in chili cultivation. Symptoms of Geminivirus infection are quite easy to find in chili plants because the symptoms are quite typical: mosaic, yellowing, small leaves, leaf lamina malformation, and stunting. The visual changes in leaf morphology are due to the leaf tissue structure, such as the upper and lower epidermis and the mesophyll tissue, including the palisade and sponge. There is little information about changes in the structure of chili plants infected with the Geminivirus. Therefore, this study aims to add information about changes in the leaf tissue structure of chili plants infected with the Geminivirus. The structural changes observed were palisades. Long palisades were less preferred by whiteflies, so the incidence of geminivirus disease was lower. This information is a reference for assembling chili plants with better structural resistance. The method used was a cross-section of chili leaves, double staining, and observation with a light microscope. Observations on mild symptoms showed epidermal cells shrinking. Some parts of palisade leaves were composed of two layers with shortened cells and tend to be oval. The symptom is that the upper epidermal cells were shrunken, and the palisade also tends to shorten. Severe symptoms of the epidermis on the leaves were curly, shriveled, thinned, and even dying; the palisade is shortened and sometimes looks irregular/tight. Changes in the leaf tissue of chili plants infected with the Geminivirus cause changes at the ultra-structural, cell, or tissue level, depending on the type of virus attack and the attack level.
LEAF ANATOMICAL CHARACTERS OF SEVERAL TRUE MANGROVE SPECIES Tihurua, Eka Fatmawati; Rahmawati, Kusuma; Agustiani, Esthi Liani; Ardhiyani , Marlina; Hutabarat, Prima W.K.; Nasution, Taufikurrahman; Sutikno, Sutikno; Surya, Dede; Damayanto, I Putu Gede P.; Apandi , Ismail; Dalimunthe, Syadwina H.; Martiansyah, Irfan; Junaedi, Decky Indrawan
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.802

Abstract

Anatomical characters of mangrove species have two important roles in mangrove studies that are as taxonomic supporting character and adaptation trait as a response to saline environment. Corks warts and sclereid are independent to environment, therefore those characters may be of taxonomic support. Mangrove species anatomical adaptation showed similar feature to drought stress or sclerophyll. The aim of this study is to distinguish anatomical characters of mangrove species that is taxonomical from habitat adaptation related anatomical characters. Fifteen true mangrove species from Banggai Kepulauan, Central Sulawesi and Banyuasin, South Sumatra were used to conduct this study. Paraffin method was used for leaf transversal section, while simple peeling using nitric acid was applied for epidermal section. The results showed that stomata distribution, cork warts, sclereid, and presence of water storage type were potential anatomical features for species identification. In addition, stomata type and epidermal cell walls also have the same potential but are still doubtful, therefore it will need to be studied further to ensure the useful of those characters. The use of anatomical characters for mangrove species identification, especially when they are in the vegetative stage, can quickly determine taxa at certain level. Those potential characters are such as glandular and non-glandular trichomes on the leaf surface.