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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Microbiology Indonesia Indonesian Journal of Chemistry Journal of Tropical Crop Science Makara Journal of Science Jurnal Tumbuhan Obat Indonesia
. TRIVADILA
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health

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Journal : Jurnal Ilmu Pertanian Indonesia

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Aktivitas urikase yang dihasilkan dari berbagai sel lactobacillus plantarum dan parameter kinetikanya Dyah Iswantini; Novik Nurhidayat; . Trivadila; Eka Mardiah
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 3 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Uric acid concentration could be determined by spectrophotometry method. Uric acid was oxidized into allatonin in the presence of uricase and calculated by measuring the decrease of uric acid absorbance at 293 nm. These uricase were obtained from cells of Lactobacillus plantarum. L. plantarum K. Mar. E was isolated from passion fruit skin and L. plantarum Mgs. Psmb and Mgs. Bst from mangosteen. This research was conducted to observe the activity and kinetics of uricase from various cells of L. plantarum by spectrophotometric method. The plate assay method indicated that L. plantarum produced uricase, based on the clear zone about 0,2 mm on glucose yeast peptone medium contained 0,2% uric acid. The optimum condition of uricase activity from the three different sources occured in physiological condition. Uricase activity generated from cells of L. p/antarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 0,1073; 0,0867; and 0,0842 U/ml, respectively. The kinetic parameters for uricase, determined with uric acid as the substrate. Vmax produced by L. plantarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 1,3635; 0,0316; and 0,0418 U/ml of bacterial culture, respectively and KM 0,1541; 0,0061; and0,0054 mM, respectively. Uricase activity in various bacterial cells of L. plantarum was stable until the second day.
Penentuan kinetika urikase dari sel bacillus subtilis, B. megaterium, dan B. cereus Dyah Iswantini; Novik Nurhidayat; . Trivadila; Adayani Nurjayanti
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Uric acid concentration could be determined based on the oxidation of uric acid into alantoin in the presence of uricase. Determination of uric acid concentration is needed to diagnose the occurrence of kidney disease in gout patients. The aim of the research was to study the kinetics of uricase in Bacillus subtilis, B. megaterium, and B. cereus cells using spectrophotometry method by measurement the decrease of uric acid absorbance at 293 nm. The optimum conditions of uricase activity from the three bacterial cells occured in physiological conditions and uricase activity was stable until the second day. The values of maximum velocity (Vmax) for B. subtilis, B. megaterium, and B. cereuswere 0.1642, 0.0824, and 0.0412 mM/min, respectively. The values of Michaelis-Menten constant (Km) for B. subtilis, B. megaterium and B. cereus were 0.0003, 0.0036, and 0.0036 mM, respectively. The value of catalytic constant (kkat) for B. subtilis, B. megaterium, and B. cereus were 410.5000, 171.6667, and 257.5000 mM/min ml 00=1, respectively. Based on these results, among all bacteria tested, the highest of uricase binding with substrate in B. substilis cells was observed because of the smallest of Km value and greatest of Vmax and kkat .
Aktivitas NADP(H) Oksidoreduktase pada Kultur Sel Kina (Cinchona ledgeriana Moens) Terelisitasi Maulidiyah Utami; Diah Ratnadewi; Dyah Iswantini; Trivadila Trivadila
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 4 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.4.540

Abstract

Cinchona ledgeriana Moens is an industrial plant producing secondary metabolite quinoline alkaloids. To maintain and moreover, to increase the quinoline production especially quinine, in vitro culture system through cell culture could be a potential alternative. If the use of elicitor in cell culture can increase the production of a secondary metabolite, the activity of the enzymes involved in the biosynthetic pathway of the secondary metabolite in question might be increasing. This study aimed to examine the activity of NADPH oxidoreductase in the elicitated cell culture of C. ledgeriana and to evaluate the correlation between the activity of this enzyme and the level of quinine production. The cell cultures of Cinchona were treated with abscisic acid (ABA) or paclobutrazol (PBZ), combined with sucrose, sorbitol, or mannitol in Wood Plant (WP) media, for 7 weeks on a shaker. The quinine concentration was determined using high-performance liquid chromatography (HPLC) and the enzyme activity was measured using fluorometry. The results showed that the highest enzyme activity was found in the P7M cells (PBZ 7 mg/L + mannitol 5.3 g/L + sucrose 20 g/L), followed by the A3S cells (ABA 3 mg/L + sorbitol 5.3 g/L + sucrose 20 g/L). These results correspond to their production level of the quinine alkaloids. The lowest enzyme activity was found in the cultures without elicitor. The increase of NADP(H) enzyme activity in the P7M and A3S treatments were 13.5 and 8.5%, respectively, compared to that in the control cells. Keywords: elicitation, fluorometry, NADP(H) oxidoreductase, quinoline alkaloid
Co-Authors Adayani Nurjayanti Anggia Murni Diah Ratnadewi Dyah Iswantini Eka Mardiah Hasanuddin Rizal Irma Isnafia Arief Irmanida Batubara Kamila, Elfa Aida Komar Sutriah M. Rafi Maulidiyah Utami Muammar Yulian NOVIK NOVIK Novik Nurhidayat Nurul Hiedayati Rut Novalia Rahmawati Sianipar Salsabila, Haditsah Sandra Arifin Aziz Sri Mulijani SUMINAR SETIATI ACHMADI Tamsin, Aqlia Hanna Nurfatiha Taopik Ridwan Zaenal Abidin