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Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680
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Journal : Microbiology Indonesia

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Glucose Biosensor Using Selected Indonesian Bacteria DYAH ISWANTINI; NOVIK NOVIK; . TRIVADILA
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.954 KB) | DOI: 10.5454/mi.5.1.2

Abstract

Microbial glucose sensors have been developed using Escherichia coli bacterial strains from Japan. However, there is interest in developing local bacteria as glucose sensors in Indonesia. In this research, the stability and the potential of a selected number of Indonesian bacteria as glucose biosensors was explored. Results of this study indicate that three of them, E. coli, Bacillus subtilis, and Thermus filiformis exhibit properties of high viability and stability at high temperature (30-60 ºC). Spectrophotometrical and electrochemical measurements showed significant absorbance values and highly stable current features for E. coli as indicated by its high capacity to produce glucose dehydrogenase. E. coli, B. subtilis, and T. filiformis produced currents of 3.25 µA, 0.2 µA, and 0.02 µA respectively, and E. coli also produced a much higher activity of glucose dehydrogenase. Electrochemical measurement using E. coli-modified carbon paste electrode allowed the determination of glucose concentration of up to 20 mM. Therefore, Indonesian E. coli has a high stability and can be used as a glucose biosensor
Co-Authors Adayani Nurjayanti Anggia Murni Diah Ratnadewi Dyah Iswantini Eka Mardiah Hasanuddin Rizal Irma Isnafia Arief Irmanida Batubara Kamila, Elfa Aida Komar Sutriah M. Rafi Maulidiyah Utami Muammar Yulian NOVIK NOVIK Novik Nurhidayat Nurul Hiedayati Rut Novalia Rahmawati Sianipar Salsabila, Haditsah Sandra Arifin Aziz Sri Mulijani SUMINAR SETIATI ACHMADI Tamsin, Aqlia Hanna Nurfatiha Taopik Ridwan Zaenal Abidin