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All Journal Jurnal Fitopatologi Indonesia Indonesian Journal of Agricultural Science Buletin Plasma Nutfah JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agriprima : Journal of Applied Agricultural Sciences Jurnal Entomologi Indonesia Jurnal Pusat Inovasi Masyarakat
Bonjok Istiaji
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor, Kampus Darmaga, Bogor 16620
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Social Sciences

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Unveiling alliinase gene candidates in shallots using resistance gene motif-based degenerate primers Herlina, Lina; Istiaji, Bonjok
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.224274-283

Abstract

Shallots are integral to Indonesian daily life, with annual production reaching two million tons. However, the superior varieties are often susceptible to disease, highlighting the need for new resistant varieties. Understanding the genetic basis of disease resistance is crucial for breeding efforts aimed at developing new varieties. Alliinase, an enzyme crucial for garlic defense, is a potential candidate for enhancing shallot resistance. This study aims to identify alliinase gene candidates in shallots using degenerate primers. Genomic DNA from the Bima Brebes genotype was isolated, and degenerate primers successfully amplified 600–800 bp fragments. Three sequences were selected for further analysis, with one sequence showing high similarity to known resistance genes. Multiple sequence alignment revealed characteristic resistance gene motifs, supporting their candidacy as resistance genes. Phylogenetic analysis grouped these sequences with known resistance genes, further supporting their potential. These findings provide valuable insights for breeding programs aiming to enhance resistance in shallots through a molecular breeding approach. The identified alliinase gene candidates can be used to develop disease-resistant shallot varieties. Incorporating these genes into breeding programs can enhance resistance, improving yield and stability in shallot production.
Co-Authors ABDUL MUNIF Ahmad Aripin Naek Lubis1 Ahmad Yuda Pratama Ainur Rosidah Ali Wafa Alisya Talita Papona Amanatillah, Niky Elfa Amanda Mawan Andika Septiana Suryaningsih Anna Ardillah Sanmas Arda Ardella Awang Maharijaya Belia Elgasari Bonny PW SOEKARNO DAMAYANTI BUCHORI DEWI SARTIAMI Dhika Kintania Primadani Dian Herawati DJOKO PRIJONO Dodin Koswanudin Dyas Almira Rahmadhani Efi Toding Tondok Efriani, Nurul Farida Hermanu Triwidodo I Gusti Bagus Wiksuana Indah Sukmawati Irmansyah Kasmiatun Kasmiatun Lilik Retnowati Lina Herlina Lina Herlina Lina Herlina Lina Herlina Lufi Diah Pratiwii MAWAN, AMANDA Muhammad Gian Gianggi Pratama Nadia Fauzana Niky Elfa Amanatillah Nurul Farida Efriani Prastowo Prastowo PURNAMA HIDAYAT Reflinur Reflinur Retnowati, Lilik Rifka Lailatul Hikmah Risda Rizkillah, Risda Rizky Nazarreta Rizky Nazarreta, Rizky Rosyid Amrulloh Ruly Anwar Saiful Akhyar Lubis Satyanto Krido Saptomo Sobir Sobir Suryo Wiyono Sutoro Sutoro Swastiko Priyambodo, Swastiko Titah Nurhawati Widodo Widodo Willy Bayuardi Woro Anggraitoningsih Noerdjito