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All Journal Jurnal Keterapian Fisik Jurnal Biologi Tropis Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Jurnal Riset Kesehatan Nasional ad-Dawaa : Journal of Pharmaceutical Sciences BIO-EDU: Jurnal Pendidikan Biologi Udayana Journal of Social Sciences and Humanities (UJoSSH) Jurnal Pijar MIPA JRKN Jurnal Pengabdian Kepada Masyarakat Itekes Bali Berita Biologi
Putu Indrayoni
Institut Teknologi Dan Kesehatan Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Liver Histology of Male Rat (Rattus norvegicus) Feed L-Carnitine Supplement Padmiswari, Anak Agung Istri Mas; Indrayoni, Putu; Wulansari, Nadya Treesna
Jurnal Pijar Mipa Vol. 19 No. 4 (2024): July 2024
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v19i4.6900

Abstract

Obesity is a condition where the body is overweight due to fat accumulation, each exceeding 20-25% of body weight for men and women. L-carnitine is a nutrient that plays a role in transporting fat to the mitochondria to be burned and converted into energy for the body. Sufficient L-carnitine allows the body to burn fat at optimal levels. This research aims to determine the side effects of fed L-carnitine supplements against liver histology. This study used a completely randomized design (CRD) with four groups comprising six albino rats. The treatments were fed orally by distilled water 1 ml/day (Control), P1 fed by L-carnitine supplement dose 100 mg/kg body weight orally 1ml/day, P2 fed by L-carnitine supplement dose 150 mg/kg body weight orally 1ml/day, P3 fed by L-carnitine supplement dose 200 mg/kg body weight orally 1ml/day. Treatments were given to albino rats every day for 90 days. Variables observed measured were lipid degeneration, central vein dilation, and pyknotic nuclei. Data were analyzed with the statistical program (SPSS 22.0 for Windows) with One Way ANOVA. The results showed that feeding L-carnitine supplements in high doses for long periods can damage the liver cells of male rats. The microscopic liver damage statistical analysis showed significant differences (P<0.05) between the control and treatment groups.
The Effect of Acupuncture Therapy on the Concentration Level of Health Students Ayu Anom Rastiti, Ida; Ayu Suptika Strisanti, Ida; Rosa Tri Anggaraeni, Komang; Indrayoni, Putu
Jurnal Keterapian Fisik Jurnal Keterapian Fisik Volume 9 Issue 2 Year 2024
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/jkf.v9i2.446

Abstract

Background: Health students face various challenges during their studies. The demands of mastering clinical skills and the ability to build therapeutic relationships with patients require high concentration from health students. So it is important for health students to have good concentration during the learning process so that students' mastery of clinical skills can be achieved optimally. Acupuncture is a therapeutic modality that has been proven to have a function to improve individual cognitive abilities. This study aims to identify the effectiveness of acupuncture therapy in improving the concentration of health students. Methods: The research sample in this study is 60 undergraduate nursing students of the Institute of Technology and Health Bali who are divided into an intervention group (n = 30) and a control group (n = 30). The instrument that will be used in this study is the Grid Concentration test to assess the level of student concentration. Results: There was a significant increase (p < 0.05) in the concentration score in the treatment group, where before acupuncture the concentration score was (median = 14) and after acupuncture, the concentration score became (median = 20.50). Conclusion: According to the results above, it proves that acupuncture therapy is effective in improving student concentration and can directly optimize working memory capacity.
Phytochemical screening of mint leaves (Mentha sp.) as an innovation in biology learning Padmiswari, Anak Agung Istri Mas; Wulansari, Nadya Treesna; Harditya, Kadek Buja; Indrayoni, Putu; Dira, Made Asmarani
BIO-INOVED : Jurnal Biologi-Inovasi Pendidikan Vol 8, No 1 (2026): February 2026
Publisher : Master Program of Biology Education, Universitas Lambung Mangkurat, Banjarmasin, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/bino.v8i1.23727

Abstract

Phytochemistry-based practicums have the potential to strengthen students' cognitive understanding, psychomotor skills, and affective attitudes. This study examines the effectiveness of phytochemistry testing practicums using mint leaf (Mentha sp.) ethanol extract on the learning outcomes, psychomotor skills, and attitudes of biology students. The research subjects consisted of 30 second-year students selected using purposive sampling, as they had already completed the basic pharmaceutical biology course. A pre-experimental one-group pretest–posttest design was applied, with instruments including a learning outcome test, observation sheets, and a response questionnaire. The phytochemical screening results confirmed the presence of flavonoids, alkaloids, saponins, tannins, and terpenoids, which provided clear and easily observable visual indicators. Statistical analysis showed a significant increase in post-test scores compared to pre-test scores (p = 0.001). Observations revealed good performance in laboratory skills such as procedural accuracy, work safety, and teamwork, although color interpretation required further improvement. Questionnaire responses indicated that students perceived the practicum positively, particularly in terms of motivation, relevance to everyday life, and integration of theory with practice. These findings indicate that mint leaf-based phytochemistry practicums effectively support student learning, with an average score of 80.4 in the cognitive domain (Good–Very Good), 82 in the psychomotor domain (Good), and 85 in the affective domain (Very Good). The use of local plants as a practical medium also provides a contextual, low-cost, and innovative approach to biology education, with strategic potential to improve the quality of laboratory-based learning.Abstrak. Praktikum berbasis fitokimia berpotensi memperkuat pemahaman kognitif, keterampilan psikomotor, dan sikap afektif mahasiswa. Penelitian ini mengkaji efektivitas praktikum uji fitokimia menggunakan ekstrak etanol daun mint (Mentha sp.) terhadap hasil belajar, keterampilan psikomotor dan sikap mahasiswa biologi. Subjek penelitian terdiri dari 30 mahasiswa tahun kedua yang dipilih dengan teknik purposive sampling, karena telah menyelesaikan mata kuliah Biologi Farmasi Dasar. Desain penelitian yang digunakan adalah pra-eksperimental dengan rancangan one-group pretest–posttest, menggunakan instrumen berupa tes hasil belajar, lembar observasi, dan angket respon. Hasil skrining fitokimia mengonfirmasi adanya flavonoid, alkaloid, saponin, tanin, dan terpenoid yang memberikan indikator visual jelas dan dapat diamati dengan baik. Analisis statistik menunjukkan peningkatan signifikan pada nilai post-test dibandingkan pre-test (p = 0,001). Observasi menunjukkan keterampilan laboratorium mahasiswa berada pada kategori baik, khususnya dalam ketepatan prosedur, keselamatan kerja, dan kerja sama tim, meskipun interpretasi warna masih perlu ditingkatkan. Respon angket menunjukkan bahwa mahasiswa menilai praktikum ini secara positif, terutama dalam hal motivasi, relevansi dengan kehidupan sehari-hari, serta integrasi teori dengan praktik. Temuan ini menunjukkan bahwa praktikum fitokimia berbasis daun mint efektif mendukung pembelajaran mahasiswa dengan memperoleh rata-rata nilai 80,4 pada domain kognitif (Baik–Sangat Baik), 82 pada domain psikomotor (Baik), dan 85 pada domain afektif (Sangat Baik). Pemanfaatan tanaman lokal sebagai media praktikum juga menyediakan pendekatan kontekstual, berbiaya rendah, dan inovatif dalam pendidikan biologi, dengan potensi strategis untuk meningkatkan kualitas pembelajaran berbasis laboratorium.
SKRINING FITOKIMIA DAN POTENSI PROBIOTIK KOMBUCHA DAUN SIRSAK (Annona muricata) DAN KAYU SECANG (Caesalpinia sappan) SERTA AKTIVITAS ANTIMIKROBA PADA BAKTERI PATOGEN SALURAN CERNA Sintyadewi, Putu Rima; Rabani, I Gusti Agung Yogi; Widnyani, Ida Ayu Putu Ary; Wulansari, Nadya Treesna; Indrayoni, Putu
Berita Biologi Vol 25 No 1 (2026): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2026.14345

Abstract

Tren hidup sehat semakin mendorong masyarakat untuk mengkonsumsi pangan fungsional, khususnya minuman hasil fermentasi yang kaya senyawa bioaktif serta probiotik. Kombucha merupakan salah satu minuman yang banyak diminati karena mengandung asam organik, antioksidan, dan mikroorganisme bermanfaat bagi kesehatan. Indonesia sendiri memiliki beragam tanaman herbal, termasuk daun sirsak (Annona muricata) dan kayu secang (Caesalpinia sappan), yang dikenal memiliki aktivitas farmakologis yang kuat. Penggunaan dua bahan tersebut dalam pembuatan kombucha berpotensi menghasilkan efek bioaktif yang lebih optimal. Penelitian ini bertujuan untuk menganalisis profil fitokimia, total Bakteri Asam Laktat (BAL) dan yeast, aktivitas antimikroba, serta penentuan nilai Konsentrasi Hambat Minimum (KHM) pada minuman kombucha daun sirsak dan kayu secang. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dua faktorial yaitu variasi konsentrasi daun sirsak dan kayu secang yaitu, P1 (90%:10%), P2 (80%:20%), P3 (70%:30%) dan waktu fermentasi D1 (0 hari), D2 (4 hari), D3 (8 hari), D4 (12 hari). Data dianalisis menggunakan ANOVA dan DMRT pada taraf signifikansi p < 0,05. Hasil penelitian menunjukkan bahwa variasi konsentrasi daun sirsak dan kayu secang pada kombucha memberikan pengaruh signifikan (p<0,05) terhadap aktivitas antibakteri E. coli dan S. aureus pada kombucha daun sirsak dan kayu secang. Formulasi P3D3 dengan komposisi 70% daun sirsak dan 30% kayu secang yang difermentasi selama 8 hari disimpulkan sebagai formulasi terbaik, yaitu berpotensi sebagai minuman probiotik dengan total BAL dan yeast masing-masing sebesar 2,0 × 10⁶ CFU/mL dan 3,2 × 10⁶ CFU/mL, aktivitas antimikroba tertinggi dengan zona hambat terhadap E. coli dan S. aureus masing-masing 12,7 mm dan 15,7 mm, dengan KHM terhadap E. coli dan S. aureus adalah 40% dan 20%.  Skrining fitokimia kombucha daun sirsak dan kayu secang menunjukkan adanya senyawa alkaloid, flavonoid, tanin, fenol, steroid dan saponin yang diduga kuat berkontribusi terhadap antivitas antibakteri.
Co-Authors AA Istri Mas Padmiswari Anjasmara, Dewa Gede Anom Asthadi Mahendra Bhandesa, Asthadi Mahendra Ayu Anom Rastiti, Ida Ayu Suptika Strisanti, Ida Dira, Made Asmarani I Gusti Bagus Teguh Ananta Ida Ayu Manik Damayanti Ida Ayu Putu Ary Widnyani Kadek Buja Harditya Kartiningsih, Ni Luh Putu Kusuma Negara, I Gusti Ngurah Made Made Rismawan Nadya Treesna Wulansari Nadya Treesna Wulansari Pande P Elza Fitriani Putu Rima Sintyadewi Rabani, I Gusti Agung Yogi Rosa Tri Anggaraeni, Komang Sri Dewi Megayanti Widnyani, Ida Ayu Putu Ary Wulansari, Nedya Treesna