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All Journal Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Farmasi BIOEDUPAT: Pattimura Journal of Biology and Learning Rumphius Pattimura Biological Journal
Syahran Wael
Program Pendidikan Biologi, Jurusan PMIPA FKIP Universitas Pattimura Ambon, Jl. Dr. Tamaela, Kampus Universitas Pattimura Ambon
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Rumphius Pattimura Biological Journal

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ANALYSIS OF VITAMIN C IN TEA POWDER FROM SEAGRASS LEAVES (Enhalus acoroides) Mamonsary, Christian; Makatita, Audrey Louise; Wael, Syahran; Tuapattinaya, Prelly Marsel Jolanda
RUMPHIUS Vol 5 No 2 (2023): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv5i2p047-051

Abstract

The seagrass species Enhalus acoroides is able to live widely, especially on smooth, muddy substrates, but is also able to grow on rocky substrates. It is often found growed together with other types of seagrass, thus forming monospecific vegetation in various habitats. The aim of this research is to determine the amount of vitamin C content made from seagrass leaves. The object in this research was Enhalus acoroides seagrass leaf tea taken from the beach of Suli Village using purposive sampling of 1.5 kg. Seagrass tea is made in the Basic Chemistry Laboratory, Pattimura University. Vitamin C analysis was carried out at the Biochemistry Laboratory, Pattimura University. Vitamin C levels in seagrass leaf tea vary for each repetition or observation sample. In sample code C1, the sample weight (g) is 10.0520, ml Titran(I2) 8.2, Vitamin C (Mg) 7.216 and vitamin C content is 0.071%, for sample code C2 the sample weight (g) is 10, 0740, ml Titran(I2) 10.3, Vitamin C (Mg) 9.064, vitamin C content of 0.090%. Meanwhile, the average amount of vitamin C is 8.14 Mg and has a vitamin C content of 0.08%. The average value of vitamin C is 8.14 Mg, while the amount of vitamin C in seagrass leaves is 0.08%. So it is very possible that Enhalus acoroides seagrass can be used as a new food preparation that is rich in nutritional content.
POTENTIAL OF HOTONG (Setaria italica (L.) P. Beauv) FROM BURU ISLAND, INDONESIAN, MALUKU PROVINCE AS A FLOUR-BASED FOOD Wael, Syahran; Wael, Ahmed; Wael, Anourine; Nusaly, Windy Natalia
RUMPHIUS Vol 5 No 2 (2023): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv5i2p052-056

Abstract

Hotong (Setaria italica (L.) P. Beauv) is a type of food plant that is used by the people of Buru Island, Maluku. The hotong is a plant whose contents are similar to the rice plant. Hotong seeds have quite high nutritional content, namely containing 11.18% protein, 2.36% fat, 73.36% carbohydrates, 11.78% water, and 1.32% ash. The energy produced per 100 grams of hotong seeds is 359 calories. Hotong plants can be used as an alternative commodity in a carbohydrate-produced food diversification program. Various local carbohydrate source commodities can be produced into flour to substitute wheat needs, save the country's foreign exchange and increase food security. Based on the availability of raw materials and prices, hotong has the most potential to be processed into flour. Hotong research results are the most numerous among carbohydrate source commodities. Until now, the name has not been able to be utilized by the public, compared to rice and wheat. This is one consideration that hotong flour can improve the image and interest of users. Hotong flour, with various technological variants, can be used for various flour-based food products, but nationally the real impact on reducing wheat consumption is still not significant. Therefore, it is necessary to discuss the prospects and potential for its use in the food industry.
Potential of Hotong Flour (Setaria italica (L.) Beauv) from Buru Island in Reducing Blood Cholesterol Levels in Wistar Rats with Diabetes Mellitus Mose, Windi; Wael, Syahran; Salmawati, Mimi; Wahyudi, Didik; Astuti, Pudji
RUMPHIUS Vol 6 No 1 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i1p001-005

Abstract

Hotong flour (Setaria italica) can be used as a functional food as therapy in the treatment of diabetes mellitus by reduce cholesterol levels in the blood. Hotong is a rice-like food crop that is usually consumed by the people of Buru Island. Hotong seeds have quite high nutritional content, namely containing 11.18% protein, 2.36% fat, 73.36% carbohydrates, 11.78% water, and 1.32% ash. The energy produced per 100 grams of hotong seeds is 359 calories. Hotong plants can be used as an alternative commodity in a carbohydrate-producing food diversification program. This study aims to determine the potential dose of hotong flour in treating diabetes mellitus by reducing cholesterol levels in the blood of rat. This research used 24 wistar rats animals which were divided into 6 groups which were given hotong flour for 21 days and the cholesterol levels were evaluated on the 7th, 14th, and 21st day. Determination of rat blood cholesterol levels using the enzymatic method. Hotong flour can reduce blood sugar levels at doses of 1 g/kg bw and 1.5 g/kg bw in rat that have been made diabetic by examination on days 7, 14 and 21 days.
OPTIMIZATION OF PROTOPLAST ISOLATION FROM DIFFERENT TYPES OF SAMPLES Mose, Windi; Wael, Syahran
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p056-061

Abstract

Isolation of protoplasts in several plant species still requires optimization to produce protoplasts that are viable and can be used for further analysis. This research aims to optimize protoplast isolation protocols in several plants: Orchid Dendrobium macrocarpus, potato Solanum tuberosum, and microalgae Phaeodactylum tricornutum, Botryococcus braunii, and Spirulina sp. The results showed that in vitro explant selection had higher sample uniformity, and a combination of enzyme solutions could be used to increase the effectiveness of protoplast isolation on D. macrocarpus, S. tuberosum, and Spirulina sp. This study provides information about protoplast isolation techniques and testing their viability as an attempt for plant breeding through micropropagation.
INTEGRATION OF PROJECT BASED LEARNING (PjBL) AND PICTURE AND PICTURE (PaP) LEARNING MODELS ON PROCESS SKILLS, CRITICAL THINKING AND LEARNING OUTCOMES OF CLASS X IPA STUDENTS AT SMA MUHAMADIYAH MASOHI AND SMA NEGERI 15 CENTRAL MALUKU Tuanany, Nahriah; Wael, Syahran; Tuaputty, Hasan
RUMPHIUS Vol 5 No 1 (2023): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv5i1p007-011

Abstract

Project based learning (PjBL) is a learning model that uses problems as a first step in collecting and integrating new knowledge based on real experience, Picture and picture (PaP) is a model using media in the form of images so that students are more focused and learning conditions pleasant. Integration between these two learning models to complement each other's strengths and cover each other's weaknesses so that what is expected after the learning process can be achieved, Integration of project based learning (PjBL) and Picture and Picture (PaP) learning models as independent variables, process skills, critical thinking and learning outcomes as the dependent variable. The aim of the study was to determine the effect of the integration of Project Based Learning (PjBL) and Picture and Picture (PaP) learning models on the ability of processing skills, critical thinking and learning outcomes of class X IPA SMA Muhamadiyah Masohi and SMA Negeri 15 Maluku Tengah. The research method is Quasi Experiment with 2 control classes (lecture method), 2 experimental classes (PjBL & PaP integration). The results showed that there was an increase in learning outcomes (87.08), process skills (85.14) critical thinking (84.20) students of class X IPA SMAN 15 Maluku Tengah, increased learning outcomes (83.10), process skills (83 .93), critical thinking (80.00) class X IPA SMA Muhamadiyah Masohi. The LSD test shows that there is an effect of integration of PjBL & PaP on process skills with critical thinking (0.020) Class X IPA 2 SMA Muhamadiyah Masohi, there is an effect of integration of PjBL & PaP on process skills with learning outcomes (0.014), and on learning outcomes with critical thinking (0.030). The conclusion is that there is an influence of the integration of Project Based Learning (PjBL) and Picture and Picture (PaP) learning models on the ability of processing skills, critical thinking and learning outcomes of class X IPA SMA Muhamadiyah Masohi and SMA Negeri 15 Maluku Tengah.
THE INFLUENCE OF INTEGRATED GUID INQUIRY LEARNING MODEL TECHNOLOGICAL PEDAGOGICAL CONTENT KNOWLEDGE CONCEPT ORGANIZATIONAL SYSTEMS OF LIVING THINGS ON METACOGNITIVE SKILLS AND CRITICAL THINKING ABILITY OF STUDENTS JUNIOR HIGH SCHOOL (SMP) BURU Nurjani, Siti; Awan, Ali; Wael, Syahran
RUMPHIUS Vol 4 No 1 (2022): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv4i1p004-007

Abstract

The guided inquiry learning model integrated with Technological Pedagogical Content Knowledge is a combination of learning models and approaches that utilize technology in the learning process. This study aims to improve students metacognitive skills and critical thinking skills. The material taught in this study is the system of living organizations (cells). This research was conducted in February 2021 in Buru District, Maluku Province. This research was conducted in three schools namely SMP Negeri 5 Buru, SMP Negeri 9 Buru and SMP Negeri 16 Buru, by using a quasi-experimental design type. The populations of this study were VII.a and VII.b at SMP Negeri 5 Buru, SMP Negeri 9 Buru and SMP Negeri 16 Buru, each with a total of 66 students. Data analysis used the Non-Parametric test with the Chi-Square test. The results of this study indicate that, in the metacognitive study there was no effect on SMP Negeri 5 Buru experimental and control classes and experimental class SMP Negeri 9 Buru with a sig > 0.05, while in the control class SMP Negeri 9 Buru and experimental and control classes at SMP Negeri 16 Buru the sig <0.05 it can be concluded that it has an effect. On critical thinking skills at SMP Negeri 5 Buru, SMP Negeri 9 Buru and at SMP Negeri 16 Buru in the experimental and control class the value is sig <0.05. Based on these data, it can be concluded that there is an influence of the guided inquiry learning model integrated with Technological Pedagogical Content Knowledge on students' critical thinking skills.
IDENTIFICATION AND DENSITY OF MOSQUITO SPECIES THAT CAUSE FILARIASIS DISEASE IN TANIWEL WEST SERAM DISTRICT Senen, Yan Berlin; Mose, Windi; Rehena, Johanis Fritzgal; Wael, Syahran
RUMPHIUS Vol 5 No 1 (2023): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv5i1p001-006

Abstract

The purpose of this study was to look at the types and density of mosquitoes in the Taniwel district. Research time is August 2021. This research is descriptive in nature. where only see the type and density using the existing formula. In this study, Mansonia and Anopheles mosquitoes were found. The results showed that mosquitoes found in East Taniwel sub-district, Sohuwe Village, Lumahlatal, and Maloang found 2 genera of mosquitoes namely Mansonia and Anopheles with mansonia density in Sohuwe village 3.29%, Lumahlatan 2.83% and Maloang 2.66. while the density of Anopheles in Sohuwe village was 3.62%, Lumahlatan 3.45% and Maloang 2.66%.
ANALYSIS OF THE HEAVY METAL CHROMIUM (Cr) IN MULLETS (Mugil dussumieri) SPECIES IN THE WAI RUHU GALALA RIVER AMBON CITY Wurlianty, Benjamina; Mose, Windi; Wael, Syahran
RUMPHIUS Vol 5 No 1 (2023): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv5i1p012-017

Abstract

The heavy metal chromium (Cr) is one of the heavy metals which is toxic and h armful if it accumulates in the body of organisms at high concentrations. Heavy metals can accumulate through the food chain, namely the higher the level of the food chain occupied by an organism, the accumulation of heavy metals in the body also increases. The aim of the research was to determine the liver contamination of mullet exposed to chromanum metal. The method used in this study was the purposive sampling method. Sediment and mullet at the Basic Kimia FKIP Laboratory while to measure the content of heavy metal Cr in sediment and mullet at the Karpan Health Laboratory, Ambon 11 Desember 2022 - 18 Januari 2023 using the AAS (Atomic Absorption Spectrophotometer). The Cr content in the sediment and mullet obtained at the estuary of the Wai Ruhu Galala river, Ambon City is still below a predetermined threshold. The research results obtained from the content of heavy metal cr in the sediment which is equal to 9,2888 Ppb while in mullet it is equal to 5,8185 Ppb. The presence of heavy metal cr is caused by contaminants which may originate from community activities around this location in the form of housedold waste coated with cr metal discharged directly into the water body such as household appliaces coated with cr metal and cans paint.
EFFECTIVENESS OF SOURSOP LEAF EXTRACT (Annona muricata L.) ON THE MORTALITY OF GRAYCOOL CATERPRISES (Spodoptera litura) Salessy, Sitti; Awan, Ali; Wael, Syahran
RUMPHIUS Vol 4 No 1 (2022): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv4i1p026-033

Abstract

Annona muricata extract with a concentration of 20% during the day showed an average value of 29%, the afternoon 1:14%, whereas 40% at a concentration of 29% the morning, during the day 86%, and afternoon 1.14%. Concentration of 60% and 1.14% morning, afternoon and evening 1.43% 1.14%. Results of Kruskal-Wallis statistics on mortality of Spodoptera litura showed a significant difference between the P values ​​indicated by early spraying time (0.317), noon (0.254), and late (1,000). With treatment A.muricata extract statistical results Kruskal-Wallis P value <0.05. A.muricata extract treatment given to the mustard plant as much as 10 ml each spraying. Based on the observation and analysis of the data it can be concluded that the extract of A. muricata at a concentration of 20%, 40%, and 60% can affect the mortality of Spodoptera litura.
EVALUATION OF TEA HERBAL BASED INGREDIENTS CLOVE LEAVES (Syzygium aromaticum) OF MALUKU INDONESIA Letlora, Marsya Elsina; Baharuddin, Alifia Audy; Wael, Syahran; Jaber, Zaid Mohamad; Rehena, Johanis Fritzgal
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p078-083

Abstract

Clove (Syzygium aromaticum) is one of the famous ancient spices and was used before christ. This plant belongs to the Myrtaceae family which is believed to be able to treat a variety of diseases. Cloves grow in tropical areas and range in height from 10-20 meters and are native plants that originated in Maluku. Clove that can be processed into a drink such as one is to make herbal tea from clove leaves. The type of research used is the experimental type, which is a study conducted to determine the results of organoleptic tests and compunds on clove leaf herbal tea. This research was conducted from July 29 to August 9, 2024 at the Basic Biology laboratory of Pattimura University. The results of the study showed that clove leaf herbal tea has a unique color, aroma and taste and meets quality standards because it has been tested at the level of color, aroma and taste preference. In the color of clove leaf herbal tea, it can be seen that the one ( very like) consists of 7 panelists, the result (like) consists of 3 panelists. Aroma it can be seen that for those who answered (very liked) consisting of 5 panelists, the result of the score (like) consisted of 1 panelist and the result (moderately) consisted of 4 panelists. For the results of the taste score, it can be seen that (very liked) consisted of 1 panelist, the result of the score (liked) consisted of 4 panelists, and the result of the score (moderate) consisted of 4 panelists and for the result of the score (disliked) consisted of 1 panelist. The results of the study showed that clove leaf herbal tea has a unique color, aroma and taste and meets quality standards because it has been tested at the level of color, aroma and taste preference. In the color of clove leaf herbal tea, it can be seen that the one ( very like) consists of 7 panelists, the result (like) consists of 3 panelists. Aroma it can be seen that for those who answered (very liked) consisting of 5 panelists, the result of the score (like) consisted of 1 panelist and the result (moderately) consisted of 4 panelists. For the results of the taste score, it can be seen that (very liked) consisted of 1 panelist, the result of the score (liked) consisted of 4 panelists, and the result of the score (moderate) consisted of 4 panelists and for the result of the score (disliked) consisted of 1 panelist.
Co-Authors Audrey Makatita Awan, Ali Baharuddin, Alifia Audy Didik Wahyudi Dorlandt, Mauritius Fredy Leiwakabessy Jaber, Zaid Mohamad Letlora, Marsya Elsina Mamonsary, Christian Mimi Salmawati Mose, Windi Nimreskosu, Asna Collins Nurjani, Siti Nusaly, Windy Natalia Pattiasina, Eifan Boyke Pay, Chrismenda Pieter Agusthinus Riupassa PUDJI ASTUTI Rehena, Johanis Fritzgal Salessy, Sitti Senen, Yan Berlin Theopilus W Watuguly Tuanany, Nahriah Tuapattinaya, Prelly Marsel Jolanda Tuaputty, Hasan Wael, Ahmed Wael, Anourine Wurlianty, Benjamina