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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Reflinur
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance

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The diversity of banana cultivars in East Kalimantan based on morphological characteristic Sonja Verra Tinneke Lumowa; Widi Sunaryo; Reflinur; Dora Dayu Rahma Turista; Ruqoyyah Nasution; Yaskinul Anwar; Zenia Lutfi Kurniawati
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 7 No. 02 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i02.1936

Abstract

Banana is a plant with nutritious fruit and can help prevent stunting. Banana plants are widely cultivated in East Kalimantan, but the type characterization has not been identified. The purpose of this study was to determine the diversity of banana plants in East Kalimantan and to characterize banana plants based on morphology. This research is explorative in nature by identifying directly in the field the cultivated banana cultivars. Morphological characterization of stems, leaves and fruit was carried out based on the banana description contained in IPGRI-INIBAP/CIRAD. The research instrument is a banana morphology observation sheet which contains parameters: leaf habit, pseudostem color, predominant underlying color of the pseudostem, sap color, blotches at the petiole base, blotches color, petiole canal leaf III, color of leaf upper surface, color of midrib dorsal surface, color of leaf lower surface, color of midrib ventral surface, wax on leaves. The result shows that at East Kalimantan is found 12 types of banana cultivars were found, Talas bananas, Rutai bananas, Kepok bananas, Mas bananas, Morosebo bananas, Tanduk bananas, Susu bananas, Maulin bananas, Red bananas, Ambon bananas, Raja bananas, and Cavendish bananas.There are variations in the morphological characteristics of banana plants, leaves and fruit that can characterize each banana.
Polymorphics SSR Markers of Chilli Parental and Breeding Lines in Chilli Resistance to Pepper Yellow Leaf Curl Virus (PYLCV) Manzila, Ifa; Syukur, M.; Priyatno, Tri Puji; Reflinur; Azmi, Chotimatul; Wulandari, Astri Widia; Gunaeni, Neni; Azizah, Nur
Jurnal Hortikultura Indonesia (JHI) Vol. 12 No. 2 (2021): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.12.2.126-137

Abstract

Aksesi cabai IPBC12 telah diketahui memiliki gen ketahanan dominan terhadap PYLCV dan dapat dimanfaatkan sebagi donor gen untuk perakitan varietas cabai tahan PYLCV. PYLCV merupakan salah satu virus patogen penting pada pertanaman cabai di Indonesia. Identifikasi marka SSR polimorfik pada populasi persilangan antara IPBC12 dan varietas Yuni dilakukan untuk mendapatkan marka yang dapat digunakan untuk seleksi progeni hasil persilangan dan terpaut dengan sifat ketahanan terhadap PYLCV. Sebanyak 20 marka SSR dianalisis polimorfismenya pada dua tetua persilangan, kemudian marka yang polimorfik diuji pada galur generasi F1 dan F2. Hasil penelitian menunjukkan ada empat marka polimorfik pada kedua tetua persilangan, tetapi ketika diuji pada galur-galur keturunannya hanya 3 marka (CaBR61, CaBR64, dan CaBR98) yang polimorfik. Berdasarkan analisis marka, 14 galur F1 terkonfirmasi hasil persilangan antar aksesi IPBC12 dan varietas Yuni. Marka yang secara konsisten mendeteksi penurunan alel dari kedua tetua pada progeni F1 adalah CaBR61. Marka tersebut berpotensi sebagai marka seleksi galur-galur hasil persilangan pada tanaman cabai. Analisis molekuler pada galur-galur F2 tidak mendapatkan keterpautan antara marka dengan sifat ketahanan. Perlu analisis lebih lanjut menggunakan jumlah marka yang mencukupi dan tersebar merata dalam genom cabai untuk memetakan gen ketahanan terhadap PYLCV pada populasi persilangan antara aksesi IPBC12 dan varietas Yuni. Kata kunci: aksesi IPBC12, Capsicum annuum, seleksi berpandu marka, varietas Yuni
Confirmations on gene introgression events and hybridity for BLB resistance and yield in rice Ashan, Muh Aswad; Reflinur; Ardie, Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 3 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v51i3.51194

Abstract

A superior variety of rice with high yield and resistance to diseases is expected to meet the increasing demand for this one of the most important carbohydrate sources worldwide. Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the major biotic stresses limiting rice production. Combining BLB resistance and high-yield traits is possible through gene pyramiding. Introgression of targeted traits in the parental genotypes and hybridity confirmation in the F1 generation are important initial steps in gene pyramiding. This study aimed to confirm the presence of targeted genes in parent genotypes using specific molecular markers and to confirm the hybridity of F1 plants generated from three crosses combinations of Inpari 32 (as the female parent) and three Code-qTSN4 lines (as the male parents) using SSR markers. This study successfully confirmed the introgression of BLB-resistance genes in Inpari 32 (Xa4, Xa7, and Xa21) and in Code-qTSN4 (Xa4, Xa7). The introgression of yield-related QTL, qTSN4, was confirmed in the three Code-qTSN4 lines (A10-1, B12-2, and A16-5) by RM17483 marker. Five SSR markers (RM5, RM55, RM105, RM223, and RM561) successfully confirmed the hybridity of F1 derived from Inpari 32xA10-1 (five individuals), Inpari 32xB12-2 (one individual), Inpari 32xA16-5 (two individuals). These confirmed hybrids can be further evaluated in the F2 generation. Keywords: Gene pyramiding; molecular marker; SSR; qTSN4; Xoo.
Co-Authors Anwar, Yaskinul Ashan, Muh Aswad Chotimatul Azmi, Chotimatul Ifa Manzila M. Syukur Nasution, Ruqoyyah Neni Gunaeni Nur Azizah Sintho Wahyuning Ardie Sonja Verra Tinneke Lumowa Tri Puji Priyatno Turista, Dora Dayu Rahma Widi Sunaryo Wulandari, Astri Widia Zenia Lutfi Kurniawati