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All Journal Jurnal Ilmu Kedokteran dan Kesehatan Jurnal Kedokteran: Media Informasi Ilmu Kedokteran dan Kesehatan Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Aisyah : Jurnal Ilmu Kesehatan Nusantara Hasana Journal
Herlinawati Herlinawati
Department Of Immunology, Faculty Of Medicine, Al-Azhar Islamic University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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UJI DAYA HAMBAT EKSTRAK METANOL DAGING BUAH KAWISTA (Limonia acidissima L.) DARI DAERAH KABUPATEN DOMPU TERHADAP BAKTERI Pseudomonas aeruginosa Gde Pande Rodriawan; Ana Andriana; Herlinawati Herlinawati; Musyarrafah Musyarrafah
Jurnal Ilmu Kedokteran dan Kesehatan Vol 10, No 5 (2023): Volume 10 Nomor 5
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jikk.v10i5.9824

Abstract

Abstrak: Uji Daya Hambat Ekstrak Metanol Daging Buah Kawista (Limoniaacidissima L.) dari Daerah Kabupaten Dompu Terhadap BakteriPseudomonas aeruginosa. Tanaman kawista (Limonia acidissima L.) merupakansalah satu jenis tumbuhan obat tradisional. Masyarakat Bima Dompu cenderungmengkonsumsi buah kawista secara langsung sebagai bahan tambahan rujak dansering digunakan sebagai pengobatan diare tradisional. Daging buah Kawista(Limonia acidissima L.) diketahui mengandung flavonoid, alkaloid, dan saponin yangdapat menghambat pertumbuhan bakteri Pseudomonas aeruginosa. Tujuanpenelitian ini adalah untuk mengetahui daya hambat ekstrak metanol daging buahkawista (Limonia acidissima L.) dari daerah kabupaten Dompu terhadappertumbuhan bakteri Pseudomonas aeruginosa. Penelitian ini dilakukan dilaboratorium Riset Fakultas Kedokteran Universitas Islam Al-Azhar menggunakanmetode ekstraksi maserasi dengan pelarut metanol. Rancangan yang digunakandalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 4 kelompokperlakukan dan 2 kontrol (kontrol positif kloramfenikol dan kontrol negatifaqudest). Data yang didapatkan dianalisis menggunakan SPSS versi 23. Hasil daripenelitian ini didapatkan diameter zona hambat pada 4 kelompok perlakuan. Dayahambat terbesar adalah 41,50 mm pada konsentrasi 100%, dan daya hambat  terendah adalah 18,5 mm pada konsentrasi 25%. Berdasarkan hasil analisis yang dilakukan, didapatkan perbedaan yang bermakna antar kelompok perlakuan dengan nilai p<0.05. Ekstrak metanol daging buah kawista (Limonia acidissima L.) dari daerah kabupaten Dompu memiliki daya hambat terhadap pertumbuhan bakteri pseudomonas aeruginosa.
Comparison of Anti-Salmonella Typhi IgM Examination Results with SD Bioline and Tubex TF. Methods Herlinawati Herlinawati; Resna Hermawati; I Gede Angga Adyana
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 7, No 3: September 2022
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.202 KB) | DOI: 10.30604/jika.v7i3.1481

Abstract

Salmonella typhi is a gram-negative bacterium that is an intracellular facultative pathogen, which enters the human body and causes an acute systemic infectious disease called typhoid fever. Among the laboratory tests used to detect the presence of anti-Salmonella typhi IgM in serum are SD Bioline and Tubex TF. This study aims to determinecomparison of the results of the anti-Salmonella typhi IgM examination with the SD Bioline and Tubex TF methods. Analytical observation with cross-sectional design. This research was conducted in RSUD DR. Soedjono Selong. The time of the study was January-April 2022. The sample of this study was patients who underwent typhoid fever examination who were hospitalized at RSUD DR. Soedjono Selong as many as 60 people. This research uses the methodconsecutive sampling,Anti-Salmonella typhi IgM was examined with the SD Bioline kit using the immunochromatography method and the TUBEX TF kit using the Inhibition Magnetic Binding Immunoassay (IMBI) method. Data analysis was performed using SPSS, namely univariate test, and bivariate test with Chi-square. There were 26 (43.3%) male and 34 (56.7%) female patients. The youngest is 2 years old and the oldest is 64 years old with an average age of 25 years ± 19 years. Of the total 60 patients examined with SD Bioline, 5 (8.3%) were positive and 55 (91.7%) were negative. Meanwhile, examination with TUBEX TF found 3 (5%) positive people and 57 (95%) negative people. And there was a significant comparison between the results of the SD Bioline and Tubex TF examination with a value (p = 0.000) and a low level of conformity was obtained with a Kappa value of 0.000. A comparison of the results of the IgM anti-Salmonella typhi examination was obtained using the SD Bioline and Tubex TF . methodswith a very low degree of conformity.
Co-Authors Adib Ahmad Shammakh Adyana, I G. Angga Ana Andriana Ana Andriana Angga Atnyana Arjita, I Putu Dedy Arya Adhi Yoga Wikrama Jaya Azmi, Fahriana Dian Rahadianti Diani Sri Hidayati Ety Retno Setyowati Fahriana Azmi Gde Pande Rodriawan I Gede Angga Adyana Ida Bagus Yuda Andika Isnawati, Isnawati, Mardiana Maya Utari Maslahatun Maslahatun Moh. Maswan Musyarrafah, Musyarrafah Rachma Meilinda Rahadianti, Dian Resna Hermawati Resna Hermawati Resna Hermawati Rusmiatik Rusmiatik S. H., Diani Sabariah Sabariah Sabariah Sabariah Sabariah Sabariah Syavira Adinda Widiastuti