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Antibiotic patterns of ampicillin, streptomycin, and tetracycline in stray cats Wibisono, Freshinta Jellia; Widiasih, Dyah Ayu; Fardiansyah , Al; Isnaeni, Mutia; Mentari , Andi Oktaviana; Qurratu'ain , Seryna Hasna; jalal , Ismul; Islam, Afif Fajrul
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 14 No. 4 (2024): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v14i4.491

Abstract

This research was conducted to determine the presence of Escherichia coli bacteria in anal swabs of wild cats, determine the existence of antibiotic resistance in Escherichia coli bacteria, and determine resistance patterns in Escherichia coli bacteria. A total of 90 swab samples were taken, consisting of 30 anal swab samples from wild cats in the East Surabaya Region and 30 anal swab samples from wild cats in the West Surabaya Region. Samples were tested at the Veterinary Health Laboratory, Faculty of Veterinary Medicine, Wijaya Kusuma University, Surabaya. Samples were brought using buffered peptone water. Samples were isolated and identified using Mac Conkey Agar differential selective media. Isolates identified as Escherichia coli were then subjected to gram staining tests, biochemical tests, and sensitivity tests to determine the presence of resistance and multidrug resistance. The results of the study showed that 95.5% (86/90) of the samples contained Escherichia coli bacteria and the results of the sensitivity test showed that there was antibiotic resistance showing that 25.5% (22/86) were resistant to the antibiotic ampicillin, 19.3% (17/86) were resistant to tetracycline antibiotics, and 13.9% (12/86) were resistant to streptomycin antibiotics. The resistance test results showed that multidrug resistance in Escherichia coli bacteria taken from cat anal swabs was 6.9% (6/86).
Kajian Epidemiologi Infeksi Bovine Viral Diarrhea (BVD) pada Sapi Perah di Kabupaten Sleman Yogyakarta Primatika, Roza Azizah; Sumiarto, Bambang -; Drastini, Yatri; Widiasih, Dyah Ayu
Acta VETERINARIA Indonesiana Vol. 8 No. 1 (2020): Januari 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.423 KB) | DOI: 10.29244/avi.8.1.32-39

Abstract

Sapi perah merupakan hewan ruminansia yang menghasilkan susu untuk konsumsi sehari hari masyarakat Indonesia karena mengandung sumber kalsium yang baik bagi tubuh. Namun, saat ini kendala yang dihadapi oleh peternak adalah menurunnya produksi susu sapi perah sehingga menyebabkan kerugian ekonomi yang cukup besar. Salah satu penyakit yang menyebabkan penurunan produksi susu pada sapi perah adalah Bovine Viral Diarrhea (BVD). Bovine Viral Diarrhea telah menyebar di seluruh dunia termasuk di Indonesia. Pemerintah Indonesia belum menetapkan kebijakan vaksinasi BVD. Tujuan penelitian ini adalah untuk mengetahui seroprevalensi dan faktor resiko mengenai infeksi Bovine Viral Diarrhea (BVD) di tingkat peternak di Kabupaten Sleman Yogyakarta. Metode penelitian ini adalah dengan melakukan wawancara terhadap peternak melalui kuesioner dan metode sampling yang digunakan pada penelitian ini adalah teknik sampling tahapan ganda serta dianalisis secara univariat dan bivariat. Sampel penelitian ini adalah 96 peternak terpilih yang memiliki sapi perah dan dilakukan pengujian Bovine Viral Diarrhea (BVD) dengan metode Enzyme-linked Immunosorbent Assay (ELISA) antibodi. Berdasarkan analisis univariat, diperoleh seroprevalensi penyakit BVD pada sapi perah sebesar 56,25%. Berdasarkan analisis bivariate, hasil yang diperoleh adalah tidak terdapat hubungan antara variabel yang diuji dengan adanya penyakit BVD pada sapi perah di Kabupaten Sleman, yang ditunjukkan dengan nilai p_value > 0.05.
Prevalence and Risk Factors of Lumpy Skin Disease in Pakem District, Yogyakarta Rahmi, Siwi Meylina; Salim, Devi Andreani; Primatika, Roza Azizah; Nugroho, Widagdo Sri; Susetya, Heru; Widiasih, Dyah Ayu; Bawono, Gigih
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.30-36

Abstract

Lumpy Skin Disease (LSD) is an infectious dermatological condition affecting ruminant animals, particularly cattle, and is caused by the Lumpy Skin Disease Virus (LSDV). This virus possesses DNA genetic material derived from the Capripoxvirus genus within the Poxviridae family. The World Organization for Animal Health (WOAH) states that LSD has a mortality of 1-5% and a morbidity of 10-20% and can spread quickly if not handled properly. This study aimed to determine the prevalence of LSD and to identify associated risk factors in Pakem District, Yogyakarta. This research used a cross-sectional study. Samples were taken using a double-stage sampling technique with a total sample of 262 cattle. The study employed primary data collected through interviews with farmers. Data was analyzed descriptively and analytically. Data in the form of frequencies and percentages were analyzed using univariate methods. The relationship between risk factors and the incidence of LSD was analyzed using bivariate Chi-Square methods. The results showed that the prevalence of LSD in the Pakem District was 7.6%. The presence of ticks was identified as a risk factor for LSD disease, with a probability value of p<0.05. The association’s strength was indicated by an odds ratio (OR) of 2.64, suggesting that ticks may elevate the risk of contracting LSD by a factor of 2.64. The prevalence of LSD in Pakem District was 7.6%, and the presence of ticks 2.64 times increases the risk of LSD incidents. Control measures can be improved by carrying out livestock management in handling vectors.
Antibiotic resistance in Escherichia coli from livestock-related sources: A cross-sectional study of feces, soil, and water in a non-urban region Silalahi, Gustaf Eifel; Widiasih, Dyah Ayu; Susetya, Heru; Nugroho, Widagdo Sri; Drastini, Yatri; Putri, Khrisdiana; Gallantiswara, Putu Cri Devischa; Yudhanto, Setyo; Primatika, Roza Azizah
The International Journal of Tropical Veterinary and Biomedical Research Vol 10, No 1 (2025): Vol 10 (1) May 2025
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v10i1.46238

Abstract

Background and Aim: Antibiotics are one of the most used treatments by farmers, both in commercial and non-commercial livestock operations. The use of antibiotics in animals directly impacts the environment, which can become a significant factor in the spread of antibiotic resistance. The environment plays an essential role for both animals and humans, and contamination caused by uncontrolled environmental factors can lead to health impacts on surrounding populations. One of the contaminants that can threaten the health of animals and humans is the presence of Escherichia coli. The aim of this study was to determine the antibiotic resistance profile of E. coli in environmental samples collected from a non-urban livestock region in Playen, Gunung Kidul Regency, Yogyakarta.Materials and Methods: The data in this study consisted of fifty samples, including fishpond water, wastewater, drinking water, water source, water tank, feces, and soil. The methodology involved bacterial isolation using biochemical tests and antimicrobial resistance testing conducted through the KirbyBauer disk diffusion method.Results: The antibiotics used in this study were streptomycin, penicillin, enrofloxacin, oxytetracycline, and sulfamethoxazole. The results showed that 5% of the samples were resistant to streptomycin, 92% were resistant to penicillin, 0% were resistant to enrofloxacin, 12.8% were resistant to oxytetracycline, and 2.5% were resistant to sulfamethoxazole.Conclusion: The findings indicate a high prevalence of Penicillin-resistant E. coli in environmental samples from non-urban livestock areas, highlighting the potential risk of environmental antibiotic resistance transmission.