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Masye Wurarah
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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PENGARUH PEMBELAJARANMENGGUNAKAN STRATEGI (PBL) TERHADAP KEMAMPUAN BERPIKIR KRITIS SISWA SMA NEGERI3 TONDANO Koropit, Rifky; Wurarah, Mastje; Worang, Rantje L.
JSME (Jurnal Sains, Matematika & Edukasi) Vol 5, No 2 (2017): Jurusan Biologi
Publisher : Universitas Negeri Manado

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Penelitian ini bertujuan mengetahui pengaruh strategi Problem Based Learning terhadap kemampuan berpikir kritis siswa. Penelitian ini menggunakan dua kelas yaitu kelas eksperimen yang menggunakan strategi Problem Based Learning dan kelas kontrol yang menggunakan strategi konvensional. Analisis data menggunakan hipotesis penelitian dilakukan uji-t, uji normalitas dan uji homogenitas. Berdasarkan hasil analisis dilihat nilai Fhitung < FTabel pada taraf signifikan (α) = 0,05, maka kelompok sampel dalam penelitian ini adalah homogen yaitu:1,050 < 1,181dan Dari hasil perhitungan dalam uji hipotesis menggunakan uji-t diperoleh harga thitung = 99,3 lebih besar dari tTabel = 2,004. Hal ini menunjukkan bahwa hipotesis H1 diterima, berdasarkan hasil belajar siswa tersebut sehingga adanya pengaruh strategi PBL terhadap kemampuan berpikir kritis biologi siswa SMA Negeri 3 Tondano. Kata kunci: Berpikir Kritis, Hasil Belajar, Problem Based Learning
PENGEMBANGAN MEDIA PEMBELAJARAN INTERAKTIF BERBASIS KOMPUTER PADA SMA NEGERI 2 BITUNG Luntungan, Geraldo M.; Sumampouw, Herry M.; Wurarah, Masje
JSME (Jurnal Sains, Matematika & Edukasi) Vol 5, No 2 (2017): Jurusan Biologi
Publisher : Universitas Negeri Manado

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Penelitian ini bertujuan untuk menghasilkan produk multimedia interaktif yang layak bagi siswa tentang pokok bahasan virus. Penelitian ini merupakan Research and Development (R&D) menggunakan model ASSURE. Langkah-langkah pengembangan multimedia ini terdiri dari 1) Analyze Learners, 2) States Objectives, 3) Select Methods, Media, and Material, 4) Utilize Media and materials, 5) Require Learner Participation, dan 6) Evaluate and Revise. Di tahapan ketiga pengembang menggunakan beberapa software dengan Lectora Inspire 12.0 sebagai software utama untuk menjadikannya antar muka (interface) dengan pengguna. Pada tahapan keenam Utilize Evaluate and Revise dilakukan uji coba produk pengembangan terbatas oleh ahli media pembelajaran dan oleh ahli isi/materi menggunakan angket. Hasil penilaian terhadap prototype media interaktif berbasis komputer (MIBK) oleh ahli media pembelajaran diperoleh kategori tidak layak, perlu direvisi dengan skor persentase sebesar 56,4%. Prototype hasil revisi mendapat penilaian dari ahli media pembelajaran dan diperoleh kategori layak, tidak perlu direvisi lagi dengan skor sebesar 75,5%. Penilaian dari ahli isi/materi diperoleh bahwa prototype memiliki nilai yang sangat bagus dan tidak perlu di revisi dengan skor sebesar 97,5%. Berdasarkan hasil penilaian uji coba ahli, disimpulkan bahwa media pembelajaran yang dikembangkan layak digunakan sebagai media pembelajaran untuk guru dan siswa dalam mempelajari materi Virus di SMA Negeri 2 Bitung. Kata kunci: ASSURE, Media Interaktif berbasis Komputer, MIBK, Virus
ANALISIS BUTIR SOAL ULANGAN HARIAN BUATAN GURU MATA PELAJARAN BIOLOGI KELAS X SMA N 1 REMBOKEN Sitorus, Andini P.; Wurarah, Masye; Gedoan, Sukma P.
JSME (Jurnal Sains, Matematika & Edukasi) Vol 5, No 1 (2017): Jurusan Biologi
Publisher : Universitas Negeri Manado

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Analisis butir soal merupakan suatu proses untuk menguji kualitas setiap butir soal. Tujuan penelitian adalah untuk mengetahui kualitas soal ulangan harian pada mata pelajaran biologi pada siswa kelas X SMA Negeri 1 Remboken. Metode yang dipakai dalam penelitian ini adalah metode deskriptif. Analisis yang digunakan dalam penelitian ini meliputi aspek empirik dan teoritik. Hasil penelitian menunjukan bahwa rata-rata tingkat kesukaran soal adalah 0,68 dengan kategori sedang, rata-rata validitas 0,507 dengan kategori tidak valid, untuk pengujian reliabilitas r11 = 0,655 kategori tinggi, sedangkan daya pembeda 0,11 dengan kategori rendah dan distraktor dengan 12 butir soal (60%) distraktornya dapat berfungsi dengan baik (efektif) dan 8 butir soal (40%) distraktornyan tidak berfungsi dengan baik (tidak efektif). Kualitas butir soal objektif ulangan harian buatan guru mata pelajaran Biologi Kelas XE di SMA Negeri 1 Remboken belum terstandarisasi secara teoritik dan empirik karena tidak mengikuti kaidah penulisan soal objektif yang baik dan benar. Kata kunci: Analisis butir soal, buatan guru mata pelajaran Biologi
POTENSI ANTIBAKTERI EKSTRAK ETANOL DAUN PARE (Momordica charantia) TERHADAP BAKTERI Staphylococcus aureus Undap, Tessa; Simandjuntak, Suddin; Wurarah, Masye
JSME (Jurnal Sains, Matematika & Edukasi) Vol 5, No 2 (2017): Jurusan Biologi
Publisher : Universitas Negeri Manado

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Abstract

Daun Pare (Momordica charantia) mengandung senyawa flavonoid, saponin, tannin, triterpenoid, dan alkaloid dengan berbagai mekanisme antibakteri. Penelitian ini bertujuan menguji ekstrak etanol daun pare (Momordica charantia) dalam menghambat pertumbuhan bakteri Staphylococcus aureus dan seberapa besar ekstrak etanol daun pare (M. charantia) dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan metode Cakram. Hasil penelitian menunjukkan bahwa ekstrak daun pare (M. charantia) dapat menghambat pertumbuhan bakteri Staphylococcus aureus dengan luas zona hambat yang terbentuk pada hari pertama, konsentrasi 10% yaitu 0,2 cm2, 15% yaitu 0,3 cm2, 20% yaitu 0,4 cm2, 25% yaitu 0,5 cm2 dan 0% (kontrol negatif). Ekstrak daun pare mempunyai kemampuan menghambat pertumbuahan bakteri Staphyloccocus aureus. Kata Kunci: Daya Hambat, Esktrak Etanol, Antibakteri, Momordica charantia, Staphylococcus aureus
Media Pembelajaran Konsep Genetika Menggunakan Drosophila melanogaster Isolat Lokal Dengan Aplikasi Whatsapp Berbasis Kearifan Lokal Budaya Mapalus Herry Maurits Sumampouw; Mokosuli Yermia Semuel; Dewa Nyoman Oka; Orbanus Naharia; Masje Wurarah
Jurnal Pendidikan Biologi undiksha Vol. 8 No. 3 (2021)
Publisher : Undiksha

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The COVID-19 pandemic has changed the conventional learning structure. Learning media is a necessity for students in online learning so that learning objectives can be achieved maximally. The subject of genetics is one of the most difficult materials for students, partly because this field of biology is abstract. Research has been carried out that aims to obtain learning media for genetic concepts using the results of laboratory studies of local isolates of fruit flies. Learning media is packaged with a local wisdom approach, namely mapalus culture, and packaged for use in the what's app application. This study applies the research and development method of the ADDIE model (Analysis, Design, Development, Implementation). The test instrument used in this study is the product validity results from material experts and media experts. Research products are uploaded to the youtube channel for easy access and use through the WhatsApp application by students. The results showed that the learning media received an 85% assessment of the feasibility of the material, 83% of the feasibility of the media, and the responses of small group students included in the very good category. Learning media for the concept of genetics uses local isolate fruit flies with the WhatsApp application, based on mapalus culture, which is potential to be used as a medium for learning biology for the subject of genetics.
Biology learning resources-based research of morphology insects in Lake Tondano Masye Wurarah; Yermia Semuel Mokosuli; Sinyo Herry Sumampouw
JURNAL BIOEDUKATIKA Vol 9, No 3 (2021)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/bioedukatika.v9i3.20218

Abstract

Water hyacinth is a habitat for various types of insects in Lake Tondano. Insects that live in association with water hyacinth can be developed as a medium for learning biology. The biological characteristics of insects from Lake Tondano were developed into learning media to stimulate students' interest in learning. Students in this study are very familiar with Lake Tondano because it is a place for playing and recreation. This study aims to characterize the morphology, anatomy, and ecology of insects that live in association with water hyacinth in Lake Tondano. The method applied in this research is the research and development method of the 4D model. This research was carried out in two stages: the analysis of insects' biological characteristics in Lake Tondano and the development of biological learning media resources on Kingdom Animalia High School material. The results showed that the audiovisual learning media based on the analysis of media experts was in the high category (85.85%), material expert analysis was included in the high category (88.88%). Furthermore, the assessment by the biology teacher was high (88.67%). The test results in a small group of students, the feasibility response was high (86.2%). Based on the results of this study, this learning media is feasible to be applied in biology learning in high school for Kingdom Animalia material. The use of local biodiversity for education is influential in motivating students' interest in learning.
Inhibition of bacterial growth of Leilem leaf extract (Clerodendrum minhassae Teijsm. & Binn): Inhibition of bacterial growth of Leilem leaf extract Masje Wurarah; Yermia Semuel Mokosuli
Jurnal Biologi Tropis Vol. 22 No. 2 (2022): April - June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i2.3394

Abstract

Leilem (Clerodendrum minhassae Teism. & Binn.) is a plant endemic to Sulawesi. Leilem leaves are used as a typical food vegetable for the Minahasa tribe. However, leilem leaves were used as a medicinal plant for intestinal worms, abdominal pain and chest pain. There has been no research report on the use of leilem leaves as a source of antibacterial bioactivity. This study aims to obtain the best concentration of antibacterial Leilem leaf extract. Leilem leaves are obtained from North Minahasa. Extraction by maceration method using various solvents with a level of polarity. The test concentrations of the extract were 100 mg/L, 200 mg/L, 400 mg/L and 800 mg/L. As a positive control, clindamycin 400 mg/L was used. The results showed that the percentage of solvent yield was ethanol, which was 1.23% at 1:4 (w/v) maceration. The average diameter of bacterial growth is 12.6 mm. The results of the one way ANOVA analysis showed significant differences between test concentrations (p>0.5). Tukey's test showed that the three extracts significantly inhibited the growth of gram-positive bacteria Staphylococcus aureus strain ATCC 25923. The n-hexane extract showed the best antibacterial activity, followed by ethanol extract and ethyl acetate extract. Leilem leaves are potential to be developed as a source of antibacterial bioactive.
ANTIBACTERIAL ACTIVITY OF EXTRACTS OF LEAVES OF LEILEM (Clerodendrum minahassae Teijsm. & Binn.) ON GROWTH GRAM - POSITIVE BACTERIA Staphylococcus aureus strains ATCC 25923 Jefry Oldy Raturandang; Dintje Pendong; Masje Wurarah
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

Leaf leilem (Clerodendrum minahassae Teism. & Binn.) commonly used by the Minahasa as food and traditional medicine for treating intestinal worms, abdominal pain, and pain in the chest. Gram-positive bacteria Staphylococcus aureus ATCC 25923 strain is a type of bacteria that is found in humans and can be the cause of the emergence of the disease. This research aims to know the types of solvents are most effective and how the antibacterial activity of extracts of leaves of leilem on the growth of the bacteria Staphylococcus aureus strains ATCC 25923. The solvent used is ethanol is polar, ethyl acetate which is semi polar, and n-heksan which is non polar. Preliminary research has been conducted on konsenterasi extract with 100 ppm 200 ppm 400 ppm and 800 ppm, the antibacterial activity of the most optimal at 800 ppm so the data will be used for the analysis in this study was konsenterasi 800 ppm. Deuteronomy was done by as much as 3 times. Positive control using antibiotics clindamicyn 400 ppm and negative controls using aquades and 10% DMSO. Isolation of bacteria using scratch in zig-zag and to test the antibacterial activity of using diffusion well in order. Results of the study showed that the percentage yield of solvent is ethanol that is 1,2329% in maceration 1:4. Drag the diameter of most bacteria are grown on ethyl acetate extract polar spring with a grade average of 12.6 mm. Based on the analysis of OneWay ANOVA showed different results for real then followed by Tukey test, to see that these three types of different extract significantly in inhibiting the growth of gram positive bacteria Staphylococcus aureus strains ATCC 25923. In conclusion, namely the first ethanol is a solvent that is most effective in dissolving the leaves leilem, both of the antibacterial activity in sequence from the most minor to the most massive is the n-heksan (non-polar), ethanol (polar), and ethyl acetate (semi polar). The third conclusion in the optimum konsenterasi 800 ppm, extract n-heksan n-heksan (non-polar), ethanol (polar), and ethyl acetate (semi polar) showed antibacterial activity significantly different.
POTENTIAL ANTIBACTERIAL EXTRACTS OF MANGROVE GENUS AVICENIA ROOT AND LEAVES FROM KOMBI BEACH MINAHASA Masje Wurarah; Devitha Kalitouw; Raflen Aril Gerungan
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

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This study aimed to obtain the phytochemical content of the crude extract of leaves and roots of Avicenia spp from Kombi Beach Minahasa. Obtaining data on the activity of inhibiting the growth of infective microbes on external wounds of crude extract of leaves and roots of Avicenia spp from Kombi Beach, Minahasa. To determine the concentration of minimum growth inhibition of crude extract of leaves and roots of Avicenia spp from Kombi Beach, Minahasa. Knowing the crude extract (crude extract) leaves and roots of Avicenia spp originating from the Minahasa Kombi Beach is bactericidal or bacteriostatic. This research consisted of extraction, phytochemical analysis and antibacterial activity test. The results showed that the dominant secondary metabolites in the root and leaf extracts were alkaloids, flavonoids, saponins and tannins. The chloroform extract inhibited the growth of bacteria in infected wounds more than the ethanol and n-hexane extracts, at concentrations of 15 and 30 ppm for the test solution of ethanol extract with an inhibitory diameter of < 7 mm and a concentration of 50 ppm with an inhibitory diameter of > 10 mm indicating that the high bioactive activity antibacterial.
Biodiversity of Drosophila sp. from the Natural Environment based on the Cytochrome Oxidase subunit 1 Gene M. Wurarah; Y. S. Mokosuli; H. M. Sumampouw
Jurnal Pendidikan IPA Indonesia Vol 12, No 2 (2023): June 2023
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v12i2.44126

Abstract

Research on insect phylogenetics is intricated by their similar morphology and significant genetic diversity. The cytochrome oxidase subunit 1 (CO1) gene is the most widely utilized mitochondrial DNA gene in the identification and study of animal molecular biodiversity. This study aims to identify and reconstruct the phylogeny of fruit flies from North Sulawesi using the cytochrome oxidase subunit 1 (CO1) gene. Fruit flies were obtained from 5 (five) areas in North Sulawesi, namely Siau (L1), North Minahasa (L2), Minahasa (L3), Southeast Minahasa (L4), and Bolaang Mongondow (L5). Fruit fly imago limbs were used as a tissue source for genomic DNA extraction. Genomic DNA extraction was carried out using the Quick-DNAâ„¢ Miniprep Kit manufacture protocol. The CO1 gene amplification was carried out by the PCR method, and the visualization of the amplicons was carried out by the 1.5% gel electrophoresis method. Nucleotide sequencing used a sequencing service at First BASE Singapore with a bidirectional sequencing method. CO1 gene amplification of each sample was visualized at 690 bp to 702 bp length. After analyzing the CO1 gene concession area using the MEGA XI program, it is found that Drosophila at L1 has 702 bp, L2 has 703 bp, L3 has 698 bp, L4 has 700 bp, and L5 has 697 bp. Based on alignment analysis using the BLAST method, it is found that the L1 fruit fly has a similarity rate of 99.29% (E=0.0) to Drosophila parapallidosa [MK659836.1]. The L2 fruit fly also has a similarity rate of 96.86% with Drosophila parapallidosa [MK659836.1]. The L3 fruit fly has a similarity level of 94.94% with Drosophila parapallidosa [MK659836.1]. The L4 fruit fly has a similarity rate of 94.43% with Drosophila parapallidosa [MK659836.1]. However, the L5 fruit fly shows a similarity rate of 96.86% with Drosophila rubida [EU493593.1]. The reconstruction results with the MEGA XI program using the Minimum Evolution model obtain two monophyletic groups where the fruit fly in Bolaang Mongondow is in a monophyletic group different from other fruit flies. The results of this study prove the variation in fruit fly species in North Sulawesi based on the identification of the CO1 gene.
Co-Authors Dany Posumah Devitha Kalitouw Dewa Nyoman Oka Dintje Pendong Gedoan, Sukma P. H. M. Sumampouw Herry Maurits Sumampouw Jefry Oldy Raturandang Jemmy L. Sumakul Jemmy Sumakul Kandowangko, Sandy Koropit, Rifky Luntungan, Geraldo M. Meity Nelltje Tanor Migau, Natalina Mokosuli Yermia Samuel Orbanus Naharia Pasaribu, Vera Laspita Raflen Aril Gerungan Regita Bala Riviani Yubelin Rompas Sinyo Herry Sumampouw Sitorus, Andini P. Suddin Simandjuntak Sukmarayu P. Gedoan Tuegeh, Reinny Silvana Undap, Tessa Utari Satiman Worang, Rantje L. Yubellian Yoru