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Edy Kurniawan
Politeknik Medica Farma Husada Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Physics Public Health Other

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Isolate and Characterization of Lactic Acid Bacteria (LAB) in Local Nira as Probiotic Starter Candidates Aini Aini; Jumari Ustiawaty; Edy Kurniawan; Alfin Maulana
Jurnal Biologi Tropis Vol. 22 No. 4 (2022): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i4.4429

Abstract

Probiotics are dietary supplements that contain lactic acid bacteria (BAL), which are capable of converting sugars (including lactose). Probiotics are used in the prevention of infectious diseases. Nira is often used as an ingredient to make alcoholic beverages (tuak) and sugar, but it has not been able to increase the economic value of sap water. The purpose of this study was to isolate lactic acid bacteria in sap as a probiotic candidate. The method in this study was carried out by isolating lactic acid bacteria in sap with a dilution series of 10-0, 10-1, 10-2, 10-3, 10-4, 10-5, and 10-6, then pipetting 1 ml into a cup petri dish and incubating in an anaerobic jar equipped with Anaerocult for 2 x 24 hours. Then macroscopic and microscopic observations were made and sugar and catalase tests were carried out to determine the type of bacteria found. The results of the isolation and characterization of bacteria in the sap were found to be 4 isolates, namely isolate 1A, which is Bacillus coagulans bacteria. Isolate 1B was a Bacillus licheniformis bacteria. Isolates 2A and 2B are bacteria from the genus Lactobacillussp.
Isolate and Characterization of Lactic Acid Bacteria (LAB) in Local Nira as Probiotic Starter Candidates Aini Aini; Jumari Ustiawaty; Edy Kurniawan; Alfin Maulana
Jurnal Biologi Tropis Vol. 22 No. 4 (2022): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i4.4429

Abstract

Probiotics are dietary supplements that contain lactic acid bacteria (BAL), which are capable of converting sugars (including lactose). Probiotics are used in the prevention of infectious diseases. Nira is often used as an ingredient to make alcoholic beverages (tuak) and sugar, but it has not been able to increase the economic value of sap water. The purpose of this study was to isolate lactic acid bacteria in sap as a probiotic candidate. The method in this study was carried out by isolating lactic acid bacteria in sap with a dilution series of 10-0, 10-1, 10-2, 10-3, 10-4, 10-5, and 10-6, then pipetting 1 ml into a cup petri dish and incubating in an anaerobic jar equipped with Anaerocult for 2 x 24 hours. Then macroscopic and microscopic observations were made and sugar and catalase tests were carried out to determine the type of bacteria found. The results of the isolation and characterization of bacteria in the sap were found to be 4 isolates, namely isolate 1A, which is Bacillus coagulans bacteria. Isolate 1B was a Bacillus licheniformis bacteria. Isolates 2A and 2B are bacteria from the genus Lactobacillussp.
Isolate and Characterization of Lactic Acid Bacteria (LAB) in Local Nira as Probiotic Starter Candidates Aini Aini; Jumari Ustiawaty; Edy Kurniawan; Alfin Maulana
Jurnal Biologi Tropis Vol. 22 No. 4 (2022): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i4.4429

Abstract

Probiotics are dietary supplements that contain lactic acid bacteria (BAL), which are capable of converting sugars (including lactose). Probiotics are used in the prevention of infectious diseases. Nira is often used as an ingredient to make alcoholic beverages (tuak) and sugar, but it has not been able to increase the economic value of sap water. The purpose of this study was to isolate lactic acid bacteria in sap as a probiotic candidate. The method in this study was carried out by isolating lactic acid bacteria in sap with a dilution series of 10-0, 10-1, 10-2, 10-3, 10-4, 10-5, and 10-6, then pipetting 1 ml into a cup petri dish and incubating in an anaerobic jar equipped with Anaerocult for 2 x 24 hours. Then macroscopic and microscopic observations were made and sugar and catalase tests were carried out to determine the type of bacteria found. The results of the isolation and characterization of bacteria in the sap were found to be 4 isolates, namely isolate 1A, which is Bacillus coagulans bacteria. Isolate 1B was a Bacillus licheniformis bacteria. Isolates 2A and 2B are bacteria from the genus Lactobacillussp.
POTENSI HIBRID GOLD NANOPARTICLES DAN ANTIBIOTIK VANKOMISIN UNTUK MENGATASI MULTI DRUG RESISTANT BACTERIA (MDR) Nur Hikmatul Auliya; Ajeng Dian Pertiwi; Edy Kurniawan
Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram Vol. 5 No. 1 (2019): JURNAL PENELITIAN DAN KAJIAN ILMIAH KESEHATAN POLITEKNIK MEDICA FARMA HUSADA MA
Publisher : Politeknik Medica Farma Husada Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (756.006 KB) | DOI: 10.33651/jpkik.v5i1.16

Abstract

Antibiotic-resistant bacteria are responsible for the majority of infections worldwide. The ?-Lactam antibiotics work by inhibiting penicillin-binding proteins (PBPs) which are currently resistant to MRSA (Methicillin-Resistant Staphylococcus aureus). To solve this problem, drug delivery systems methods should be developed to overcome bacterial resistance. Nanoparticles offer a system which can improve bioavailability, efficacy, and specificity works by pharmaceutical drugs. In this study, hybridization between gold nanoparticles and vancomycin antibiotics was carried out followed by the characterization of hybrid by FT-IR and UV-Vis determination. Antibacterial activity testing also was done by the Kirby-Bauer method. The results showed that the vancomycin was resistance against MRSA, whereas hybridization process by gold nanoparticles increased its ability. The hybrid showed antibacterial activity against MRSA. The test showed the ability of hybrid to inhibit MRSA at the concentration of 100 ppm, 500 ppm and 1000 ppm with a clear zone of 13 mm, 24 mm and 28 mm respectively.
ACTIVITY OF ENDOFIT MOLD METABOLITES INSULATED FROM BETEL LEAVES (Piper betle L.) On FUNGUS Candida albicans Sumiatun; Aini; Edy Kurniawan; Jumary ustiawaty
Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram Vol. 6 No. 1 (2020): JURNAL PENELITIAN DAN KAJIAN ILMIAH KESEHATAN POLITEKNIK MEDICA FARMA HUSADA MA
Publisher : Politeknik Medica Farma Husada Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.984 KB) | DOI: 10.33651/jpkik.v6i1.58

Abstract

Betel (Piper betle) is a type of vines that have been long known and are used hereditary for the treatment. Fungal endophyte betel leaf producing antimicrobial substances and secondary metabolites that are used as the discovery, and production of new drugs. Candidiasis is a disease caused by Candida albicans when immune dysfunction occurs and causes diseases of the oral mucosa, the digestive tract and vagina. The use antifungal drugs from chemicals to cause side effects on candidates that many alternative treatments using natural ingredients as an antimicrobial. This research aims to isolate fungal endophyte of betel leaf (Piper betle L.) and know the activity of metabolites against the fungus Candida albicans. This research was conducted in the laboratory of Plant Physiology and Biotechnology, Faculty of agriculture, University of Mataram in May to June 2019. The research method used is Descriptive Observation. Research done by the fungal endophyte isolate of betel leaf and identify it. Samples of the betel leaves are obtained from the village of Bonjeruk, Kec. Jonggat, Kab. Central Lombok. Production of secondary metabolites of fungal endophyte was obtained with the method of fermentation and tested its activity against Candida albicans with the well diffusion method. The test germ used is obtained from the Litbangkes Laboratory of the NTB Province Hospital. The research results showed that fungal endophyte isolated from the leaves of the betel (Piper betle L.) and obtained the results of the identification of the fungal genus Cephalosporium sp and showed an inhibition zone of 30.9 mm.
Pengaruh Pemberian Suspensi Bacillus laterosporus dengan Sumber Karbon Alami Berbeda terhadap Laju Germinasi Kedelai (Glycine max) Edy Kurniawan; Idham Halid
JSN : Jurnal Sains Natural Vol 1 No 2 (2023): May
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.523 KB) | DOI: 10.35746/jsn.v1i2.312

Abstract

The increase in food demand is not in line with the increase in the amount of production and quality of agricultural products. The strategy to increase agricultural production so far has been carried out through the provision of chemical synthetic fertilizers, this strategy has a negative impact on the quality of agricultural land, namely a decrease in the essential organic elements of the soil which are needed by plants. Organic farming is a necessary solution to restore land quality and Bacillus laterosporus is a microorganism that has the ability to produce various beneficial metabolites for plant growth and maintain soil fertility. B. laterosporus is a microorganism that requires a high-quality carbon source to produce high-quality metabolites, so it requires natural carbon sources that are cheap, easy to obtain and not widely used by humans, such as old coconut water, nira and molasses. The aim of the study was to determine the difference in the germination rate of soybeans treated with B. laterosporus suspension with different natural carbon sources so that the best natural carbon source for the growth of B. laterosporus could be identified as an essential component of quality organic fertilizer. Soybean germination rate was measured and observed and compared with the control. The results showed that the provision of natural carbon sources had a good effect on the growth of the number of bacterial cells and the germination rate of soybean seeds.
IMPLEMENTASI PERILAKU HIDUP BERSIH DAN SEHAT (PHBS) SEBAGAI SENJATA UTAMA PENCEGAHAN PENULARAN COVID-19 DI SEKOLAH Edy Kurniawan; Sri Idawati; Helmina Andriani
Jurnal Pengabdian Masyarakat Sehati Vol. 1 No. 1 (2022): Jurnal Pengabdian Masyarakat Sehati
Publisher : Politeknik Medica Farma Husada Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.058 KB) | DOI: 10.33651/jpms.v1i1.386

Abstract

Tujuan kegiatan pengabdian masyarakat ini adalah Memberikan pemahaman kepada seluruh siswa tentang PHBS di sekolah untuk mencegah penularan covid-19. PHBS pencegahan penularan covid-19 meliputi pola konsumsi makanan bergizi, rutinitas olah raga, menjaga higienitas tubuh terutama tangan, hindari menyentuh wajah, selalu menggunakan masker, istirahat yang cukup, etika batuk dan bersin yang benar, menjaga kebersihan lingkungan, serta menghindari berkerumun dan menjaga jarak aman. Manfaat diselenggarakannya kegiatan ini untuk meningkatkan dan memantapkan pemahaman siswa/masyarakat sekolah tentang perilaku hidup bersih dapat mencegah serta menurunkan angka penularan covid-19 di masa pandemi. Kegiatan edukasi ini merupakan suatu upaya untuk menangani permasalahan kesehatan yang sering terjadi di lingkungan sekolah dengan target sasaran adalah guru dan siswa. Langkah-langkah pelaksanaan kegiatan adalah melakukan pertemuan dengan kepala sekolah dan perwakilan guru SMKN 1 Kediri Lombok Barat guna pengurusan perizinan melakukan kegiatan edukasi dan mendiskusikan masalah teknis pelaksanaan. Dari hasil diskusi didapatkan kesepakatan kegiatan edukasi dilaksanakan pada hari sabtu, 29 januari 2022 pukul 09.00 s/d selesai dengan target siswa kelas XII sebanyak 30 orang dengan tema Implementasi perilaku hidup bersih dan sehat sebagai senjata utama pencegahan penularan covid-19 di Sekolah. Kegiatan edukasi dan diskusi berjalan dengan lancar dimana para siswa sangat antusias dalam memperhatikan materi yang disampaikan terutama terkait pengetahuan tentang covid-19 dan pencegahannya, ada beberapa siswa yang antusias mempraktekkan cara mencuci tangan yang benar sesuai standar higienitas.
SOSIALISASI PEMANFAATAN TANAMAN BERKHASIAT OBAT UNTUK APOTEK HIDUP SEBAGAI UPAYA PENINGKATAN KUALITAS KESEHATAN MASYARAKAT Edy Kurniawan
Jurnal Pengabdian Masyarakat Sehati Vol. 1 No. 2 (2022): Jurnal Pengabdian Masyarakat Sehati
Publisher : Politeknik Medica Farma Husada Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.675 KB) | DOI: 10.33651/jpms.v1i2.435

Abstract

Kesuburan tanah dan keberlimpahan air di desa Labuapi tidak seiring dengan pemanfaatan kondisi tersebut oleh masyarakat, seperti rendahnya inisiatif dalam memanfaatkan lahan pekarangan yang kosong untuk ditanam tanaman bermanfaat dan berkhasiat obat dalam bentuk apotek hidup. Tujuan kegiatan pengabdian masyarakat ini adalah Memberikan pemahaman kepada seluruh masyarakat yang menjadi peserta kegiatan tentang jenis tanaman berkhasiat obat serta cara pengolahannya yang tepat. Manfaat diselenggarakannya kegiatan ini untuk meningkatkan dan memantapkan pemahaman masyarakat desa Labuapi tentang jenis tanaman berkhasiat obat serta cara pengolahannya yang benar. Kegiatan edukasi ini merupakan suatu upaya untuk menangani permasalahan kesehatan yang berhubungan dengan tindakan preventif dan pra kuratif, target sasaran adalah masyarakat. Langkah-langkah pelaksanaan kegiatan adalah melakukan pertemuan dengan kepala desa dan kepala dusun Labuapi timur, Labuapi utara, dan Labuapi selatan guna meminta izin melakukan kegiatan sosialisasi serta mendiskusikan masalah teknis pelaksanaan. Dari hasil diskusi didapatkan kesepakatan kegiatan edukasi dilaksanakan pada hari senin, 27 desember 2021 pukul 09.00 s/d selesai dengan target masyarakat di tiga dusun sebanyak 50 orang dengan “tema pemanfaatan tanaman lokal untuk apotek hidup sebagai upaya peningkatan kualitas kesehatan masyarakat”. Kegiatan sosialisasi dan praktik pemanfaatan tanaman berkhasiat obat berjalan dengan lancar tanpa halangan suatu apapun. Masyarakat sebagai peserta kegiatan telah mengenal dan memahami cara pengolahan tanaman yang dapat dimanfaatkan sebagai obat/herbal untuk upaya tindakan preventif dan pra kuratif, sehingga dapat menjadi solusi dalam mengatasi permasalahan kesehatan dalam masyarakat.
SOSIALISASI PEMANFAATAN DAUN SELEDRI PADA PEMBUATAN SABUN CUCI TANGAN CAIR UNTUK PENCEGAHAN PENULARAN COVID-19 Edy Kurniawan; Sri Idawati; Helmina Andriani
Jurnal Pengabdian Masyarakat Sehati Vol. 2 No. 1 (2023): Jurnal Pengabdian Masyarakat Sehati
Publisher : Politeknik Medica Farma Husada Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.678 KB) | DOI: 10.33651/jpms.v2i1.446

Abstract

Kebiasaan mencuci tangan dengan sabun menjadi suatu perilaku esensial yang perlu dibudayakan dalam upaya mencegah penularan COVID-19. Maka diperlukan alternatif bahan alami untuk pembuatan sabun cuci tangan cair yang aman digunakan dalam jangka waktu panjang, salah satunya adalah daun seledri (Apium graveolens). Tujuan kegiatan pengabdian kepada masyarakat ini adalah memberikan pengetahuan dan pemahaman kepada guru dan siswa SMKN 1 Kediri Kabupaten Lombok Barat tentang pemanfaatan daun seledri untuk pembuatan sabun cuci tangan cair. Metode sosialisasi yaitu memberikan materi menggunaan media power point, praktek cara mencuci tangan dengan baik dan benar serta diskusi.Kegiatan sosialisasi diikuti oleh guru dan siswa kelas XII berjumlah 35 orang. Sabun herbal adalah suatu jenis sabun yang umumnya berasal dari ekstrak tanaman dan minyak essensial yang memiliki aktivitas antibakteri. Jadi sabun cuci tangan cair dari ekstrak daun seledri ini ramah lingkungan dan aman digunakan dalam jangka waktu panjang. Program ini mampu memberikansolusi terhadap permasalahan lingkungan khususnya dalam pembuatan produk sabun ramah lingkungan.Pemanfaatan sabun herbal untuk mencuci tangan adalah salah satu kegiatan Pola Hidup Bersih dan Sehat (PHBS) dalam upaya pencegahan penularan covid-19 di lingkungan sekolah. Guru dan siswa sangat antusias memperhatikan materi pembuatan sabun cuci tangan dari ekstrak daun seledri dan praktek mencuci tangan.
Identification of Escherichia coli Sub Type Enterotoxigenic (ETEC) from Food Samples Using Pcr (Polymerase Chain Reaction) Technique Deby Anggryani; Edy Kurniawan; Dhika Juliana Sukmana; Jumari Ustiawaty
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5540

Abstract

Escherichia coli is a normal flora of the digestive tract of humans and animals and is important in the digestion of food. This bacteria lives in feces, can cause health problems, such as diarrhea and vomiting. This research was conducted for consideration in the prevention of diarrhea, processing, marketing of food. This study aims to determine the presence of enterotoxigenic Escherichia coli sub-type (ETEC) from food samples. This research is an explorative descriptive study, as many as 5 samples from food, food samples taken are food consumed every day and some include traditional food in Mataram City. The samples were cultured on BHI-Broth media, followed by inoculation on Eosin Methylene Blue (EMB) medium and continued with the Gram stain test. Colonies that are metallic green in colour and show the characteristics of Escherichia coli bacteria on Gram staining are then carried out for purification of the bacteria on Nutrient Agar (NA) medium, if the classification of bacteria shows the characteristics of Escherichia coli bacteria on Gram staining then proceed with the Indole/sugar-test. The results showed that of the 5 food samples isolated, only 1 sample showed positive E.coli bacteria. Then proceed to the PCR (Polymerase Chain Reaction) stage using the qualitative PCR method to determine the presence of Enterotoxigenic Escherichia coli sub-type (ETEC) bacteria in food samples. , with a target gene of 237 bp. 
Co-Authors Aini aini aini aini Ajeng Dian Pertiwi Alfin Maulana BaiqIsti Hijriani Deby Anggryani Dhika Juliana Sukmana Dwi Soelistya Dyah Jekti Eti Kurniati Hariyati,, Titi Helmina Andriani Idham Halid Indriati,, Indriati, Jumary ustiawaty Lalu Zulkifli Maruni Wiwin Diarti Nur Hikmatul Auliya Putri Faujiah Roushandy Asri Fardani Santy Pristianingrum Sri Idawati Sri Rahmawati Sumiatun Sumiatun Sumiatun ustiawaty, jumari