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All Journal Buletin Peternakan Jurnal Veteriner Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Ilmu Ternak Veteriner JURNAL GALUNG TROPIKA Jurnal Hilirisasi IPTEKS (JHI) Wahana Peternakan Jurnal Biosense Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Ananda Ananda
Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Perkembangan Sel Endometrium Domba setelah Inkubasi dalam Kolagenase dan Dikultur In Vitro dengan Estradiol dan Progesteron Ananda Ananda; Ekayanti Mulyawati Kaiin; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.755 KB) | DOI: 10.19087/jveteriner.2019.20.3.298

Abstract

The aim of the present study was to determine the optimal incubation time of collagenase on concentration, viability, quality of endometrial cell culture, and the role of estradiol and progesterone on ovine endometrial cells proliferation. Endometrium minced was incubated into collagenase with different duration of time: 1 hour, 3 hours, and 6 hours repectively. Cell concentration and viability were calculated after incubation. The quality of cell culture was observed on 1st, 3rd, and 5th day after seeding. The best incubation result was then continued with the addition of the E2 (100 pg/ mL), P4 (100 ng/mL), E2:P4 (100 pg/mL : 10 ng/mL), and E2:P4 (10 pg/mL : 100 ng/mL) into the culture medium. After nine days, cell culture was harvested by trypsinization. Concentration and cell viability were analyzed using analysis of variance, followed by Duncan test. Quality of endometrial cell culture was analyzed descriptively. Results showed that there was a significant decreased in the concentration and cell viability obtained in each treatment of collagenase incubation time and optimum treatment of endometrial cell culture was found at 3 hours. Experiment on culture of endometrial cell revealed that proliferation rate treated by E2 and P4 was better then control (P<0.05). Futhermore, additional of E2 into the culture medium even E2 alone (100 pg/mL or higher E2 combination with P4 (100 pg/mL : 10 ng/mL) showed better proliferation rate than P4 alone (100 ng/mL) or higher P4 combination (10 pg/mL : 100 ng/mL). In conclusion, suplementation of 100 pg/mL of estradiol (E2) could support better proliferation of ovine endometrial cells in vitro.
FUNGSI REPRODUKSI DAN GAMBARAN HISTOLOGI ORGAN REPRODUKSI MENCIT (Mus musculus) BETINA PASCA PEMBERIAN KOPI Ananda; Herbert Sipahutar; Meida Nugrahalia
JURNAL BIOSENSE Vol 5 No 2 (2022): Edisi Desember 2022
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.037 KB) | DOI: 10.36526/biosense.v5i2.2057

Abstract

Penelitian ini bertujuan untuk mengetahui fungsi reproduksi dan gambaran histologi organ reproduksi mencit (Mus musculus) betina pasca pemberian kopi secara oral. Parameter yang diamati adalah berat badan, berat uterus, panjang siklus estrus, daya fertilisasi, jumlah folikel primer, jumlah folikel sekunder, diameter pembuluh darah ovarium, tinggi epitel endometrium, dan ketebalan endometrium dengan lama pemberian selama 21 hari dan 42 hari untuk parameter daya fertilisasi. Dosis kopi yang digunakan dalam penelitian ini adalah setara dengan tiga cangkir kopi pada manusia yang dikonversikan menjadi 0,5 ml pada mencit dan dilakukan empat kali pengulangan. Data yang diperoleh dianalisis dengan menggunakan uji t. Hasil penelitian menunjukkan pemberian kopi memberi pengaruh yang nyata terhadap jumlah folikel sekunder ovarium mencit dan tinggi epitel endometrium mencit, serta tidak berpengaruh nyata terhadap berat badan, berat uterus, panjang siklus estrus, daya fertilisasi, diameter corpus luteum, jumlah folikel primer, diameter pembuluh darah ovarium, ketebalan endometrium, dan ketebalan miometrium.
Effect of Zinc Supplementation in the Diet on Sikumbang Janti Female Duck Performance, Carcass, Digestive Organs, and Intestinal Morphology Ridho Kurniawan Rusli; Robi Amizar; Zurmiati Zurmiati; Ananda Ananda; Arif Darmawan; Kusnadidi Subekti; Khalil Khalil
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 2 (2023): June 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i2.3209

Abstract

The research aimed to evaluate the effect of Zn supplementation in diet of Sikumbang Janti female duck on its performance, carcass, digestive organs, and intestinal morphology. The study used 96 female ducks aged 8 weeks. This research used a completely randomized design with four treatments (0, 30, 60, and 90 mg Zn/kg), each treatment was repeated four times. Performance, carcass, digestive organs, and intestinal morphology were observed. The results showed that Zn supplementation significantly (P0.05) affected body weight, body weight gain, feed conversion ratio, carcass weight, abdominal fat, and intestinal morphology, but it did not affect (P0.05) feed consumption, carcass percentage, and digestive organs. In conclusion, supplementation of 60 mg Zn/kg in the diet improved performance, intestinal morphology, and the health of visceral of Sikumbang Janti female ducks aged 8 to 16 weeks.
Sperm Longevity and Motility in Ringer’s Lactate Solution with Addition of Egg Yolk among Five Phenotypes of Kokok Balenggek Chicken Ananda Ananda; Jaswandi Jaswandi; Rusfidra Rusfidra; Harif Gusdinal
Buletin Peternakan Vol 47, No 3 (2023): BULETIN PETERNAKAN VOL. 47 (3) AUGUST 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i3.83647

Abstract

The application of cryopreservation to preserve germplasm in such specific breed requires preliminary studies, primarily related to the resistance of spermatozoa to low temperatures (4 – 5 °C) as measured by their motility and longevity. In this study, semen taken from five phenotypes of Kokok Balengeek Chicken (KBC) (Biriang, Jalak, Kinantan, Kuriak, and Taduang) was used to evaluate the effect of Ringer’s Lactate-egg yolk diluent on longevity and motility of spermatozoa. The treatments consisted of Ringer’s Lactate (RL) solution added with egg yolk at a concentration of 1% (RLKT1), 3% (RLKT3), and 5% (RLKT5). Evaluation of fresh semen showed that the spermatozoa of Jalak had the highest motility, namely 75.63 ± 0.5% (P<0.05). Post-dilution longevity and motility observations were carried out at 0, 24, 48, and 72 h, significantly decreasing each time (P<0.05). The lowest range of reduction was found in Jalak spermatozoa diluted with RL with longevity of 7.75 ± 0.70 days. Overall, the RL diluent showed the highest motility after 24 h, namely 41.13 ± 2.27%. Adding egg yolks to Ringer's Lactate solution could not maintain the motility of KBC spermatozoa when stored at 4-5°C for 48-72 h.
Exploring the Radiation Techniques in Agricultural Wastewater Management Af&#039;ida Khofsoh; Inggit Kresna Maharsih; Ananda Ananda; Ahmad Dody Setiadi; Muhammad Hamzah Solim
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 19, No 1 (2023): Vol 19, No. 1 (2023): November 2023
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2023.19.1.6889

Abstract

Radiation techniques have gained significant attention in the field of agricultural wastewater management due to their effectiveness in treating diverse contaminants. This review aims to explore the effects and applications of radiation techniques, including ultraviolet (UV), gamma-ray, and electron beam (EB). UV radiation utilizes ultraviolet light to break down organic pollutants, disinfect pathogens, and remove pesticides in agricultural wastewater. Besides, gamma radiation involves the use of ionizing radiation to interact with contaminants and induce degradation processes. Furthermore, EB radiation harnesses high-energy to degrade organic compounds in wastewater. The efficacy of radiation techniques in reducing pesticides, pharmaceutical residues, microorganisms or pathogens, and other organic pollutants has been widely demonstrated. These techniques offer advantages such as versatile applicability, precise targeting of contaminants, and the potential for water reuse in various agricultural sectors, such as crop irrigation, livestock farming, and food processing. However, optimizing process parameters, including radiation dose, dose rate, pH, and temperature, are crucial to maximize treatment efficiency. While radiation techniques have proven beneficial in numerous studies, potential environmental impacts must be addressed. Byproducts generated during radiation and their fate should be studied to evaluate their toxicity and persistence. Proper waste disposal, adherence to safety regulations, and monitoring programs are necessary to minimize risks and ensure the safe use of radiation techniques. In conclusion, UV-C radiation effectively use for surface disinfection, pathogen inactivation, certain pesticides and pharmaceutical residues degradation, while gamma-ray more effective than UV-C for microorganism sterilization and inactivation, pesticide and pharmaceutical residues degradation, as well as EB radiation has high dose rate and selective penetration, and the technique also has speed and precision, feasible for practical application. Thus, advancements in technology will further optimize the efficacy and sustainability of radiation-based wastewater treatment processes in agriculture.
PELATIHAN MANAJEMEN PAKAN DAN PEMELIHARAAN KAMBING PERANAKAN ETAWA UNTUK MENINGKATKAN KINERJA PETERNAK DIPETERNAKAN AZHAR FARM PAYAKUMBUH Rizqan Rizqan; Elly Roza; Hilda Susanty; Linda Suhartati; Ananda Ananda
Jurnal Hilirisasi IPTEKS Vol 7 No 2 (2024)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v7i2.790

Abstract

Goats are one of the small ruminants as meat and milk producers (dual-purpose), but cow's milk is widely sold in the market. Goat milk is no less nutritious than cow's milk; besides having an advantage in protein content, goat milk also contains more vitamins A and B (especially riboflavin and niacin). One of the goats widely cultivated by farmers and utilized as a source of animal protein, meat, and milk is the Ettawa crossbred goat. As researchers have widely reported, the potential of Ettawa crossbred goats is that milk producers are still very diverse, ranging between 0.45 and 2.2 kg/head/day. This is generally caused by maintenance management, which still needs to be improved. The purpose of this service is to counsel Azhar Farm farms on the management of raising Ettawa crossbred goats to get maximum productivity. The methods used are counseling, training, coaching, discussion, and evaluation of service activities. The activity results include improving farmers' ability to apply the management of raising Ettawa crossbred goats, which are dairy-type goats (milk producers), and increasing their productivity. It is hoped that this activity can motivate farmers to develop their livestock business to improve livestock health, increase population, increase production, and increase income for farmers.
Efektifitas Herba Ciplukan (Physalis angulata L.) Sebagai Imunostimulan Kekebalan Unggas Setelah Uji Tantang Virus Newcastle Desease Nurhasanah, N; Yellita, Yulia; Sabrina, S; Ananda, A
Jurnal Galung Tropika Vol 12 No 2 (2023)
Publisher : Fakultas Pertanian, Peternakan dan Perikanan Universitas Muhammadiyah Parepare

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v12i2.1074

Abstract

This research aims to determine the effectiveness of ciplukan herb (Physalis angulata L) in extract and powder preparations as an immunostimulant against the immunity of broilers who have received vaccination and tested against the Newcastle disease virus. The materials used were 96 DOC with an average weight of 35-40 g, ciplukan herb from Luhak Nan Duo, the velogenic strain of Newcastle disease virus from Bvet Lampung, and the commercial vaccine ND Lasota. The study used a completely randomized design (CRD) with 8 treatments and 3 replications. The ciplukan herb was given for 3 days with concentrations: K(-) (without vaccine without ciplukan), K+ (vaccine only), E3 (ciplukan extract 0.35mg/10ml/head + vaccine), E2 (ciplukan extract 0.25mg/head 10ml/head + vaccine), E1 (0.15mg/10ml/head ciplukan extract + vaccine), S3 (17.5mg ciplukan powder/head + vaccine), S2 (12.5mg ciplukan powder/head + vaccine) and S1 (ciplukan powder 7.5mg/head + vaccine). The parameters observed in this study were antibody titer, morbidity, and mortality. The study showed that the administration of ciplukan herb positively affected low antibody titers through the efficiency of cellular defense mechanisms. This herb can also reduce chicken mortality and morbidity after challenge tests against the Newcastle disease virus.
EFEKTIVITAS EKSTRAK DAN SIMPLISIA TANAMAN CIPLUKAN (PHYSALIS ANGULATA L.) TERHADAP GAMBARAN HISTOPATOLOGI ORGAN LIMPA YANG DIINFEKSI VIRUS NEWCASTLE DESEASE (ND): Efectivity of Ciplukan Plant Extract and Simplicia (Physalis Angulata L.) on Histopathology of The Spleen Infected with Newcastle Disease Virus (NDV) Juwita Fitri, Rama; Yulia Yelita; Montesqrit; Ananda
Wahana Peternakan Vol. 9 No. 1 (2025): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v9i1.1111

Abstract

Penelitian bertujuan untuk mengetahui efektivitas sediaan simplisia dan ekstrak etanol tanaman ciplukan (Physalis angulata L.) terhadap histopatologi organ limpa ayam broiler yang diinfeksi virus Newcastle Disease (ND). Materi penelitian ini menggunakan DOC ayam broiler CP 707 berjumlah 24 ekor, tanaman ciplukan (ekstrak dan simplisia), isolat ND velogenik, Vaksin ND live komersil Medivac ND®, Ransum komplit broiler komersil. Rancangan penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan kombinasi perlakuan diperoleh sebanyak 8 perlakuan dengan 3 ulangan. Data yang diperoleh dianalisis dengan analisis of varian (ANOVA) dan dilanjutkan dengan uji Duncan taraf 5%. Hasil penelitian menunjukan bahwa terdapat perbedaan sangat nyata p<0,01 antara kelompok perlakuan K1 dan K2 dengan E1, E2, E3, S1, S2 dan S3. Kelompok perlakuan S3 menunjukan perubahan histopatologi lebih baik dibandingkan E3. Kseimpulan pemberikan sediaan simplisia dan ekstrak etanol tanaman ciplukan (Physalis angulata L.) memiliki efektivitas terhadap gambaran histopatologi organ limpa ayam broiler yang diinfeksi virus Newcastle Disease (ND) dalam bebrbagi tingkatan dosis.   Kata kunci: Physalis Angulata, Newcastle disease, Limpa
VITAMIN C SUPPLEMENTATION IN EGG YOLK CITRATE EXTENDER OPTIMIZES BANGKOK ROOSTER SEMEN QUALITY DURING COLD STORAGE Ananda; Faizal, Noval Jumatul; Jaswandi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11346

Abstract

This study aimed to evaluate the effect of different vitamin C concentrations (0, 0.1, 0.2, and 0.3 g/100 mL) in egg yolk citrate extender and storage time (0, 24, 48, 72, and 96 hours) on the quality of Bangkok rooster semen stored at 5°C. Using a split-plot-in-time design with four replications, semen quality was assessed for motility, viability, abnormality, intact plasma membrane (IPM), and longevity. Results showed a significant interaction (P<0.01) between vitamin C concentration and storage time on motility, and significant effects (P<0.05) on viability, abnormality, and IPM. The 0.2 g vitamin C dose preserved >40% motility and >50% viability for 96 hours and extended sperm longevity up to 11.25±0.95 days. These findings suggest that 0.2 g/100 mL vitamin C is the optimal concentration for preserving rooster semen quality during short-term cold storage.
KINEMATICS OF SPERMATOZOA IN FRESH AND FROZEN SEMEN OF PESISIR CATTLE Ramadhan, Rio; Ananda; Hendri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11410

Abstract

This study aimed to evaluate the quality and kinematics of spermatozoa in fresh and frozen semen of coastal cattle. Semen of coastal cattle was collected using an artificial vagina from 2 males, fresh semen was tested and frozen using tris egg yolk diluent. Fresh and frozen semen of coastal cattle were evaluated using a Computer Assisted Sperm Analyzer (CASA). This study used a t-test analysis by comparing the quality and kinematics of spermatozoa of fresh semen and frozen semen of coastal cattle. The parameters observed were macroscopic, microscopic and CASA quality observations. The results showed the kinematics of fresh and frozen semen motility (83.83%; 71.68%) and the kinematics of progressive motility of fresh and frozen semen (80.81%; 59.56%). Based on the results of the t-test analysis, it was found that t count > t table, the kinematics of fresh semen spermatozoa had a significant effect on the frozen semen of Pesisir cattle on the motility of fresh semen and frozen semen, on the progressive motility of fresh semen and frozen semen. The characteristic values ​​of kinematics in fresh semen and frozen semen of Pesisir cattle were respectively velocity curvilinear (VCL) 178.94 μm/s and 108.52 μm/s; velocity straight line (VSL) 96.61 μm/s and 49.34 μm/s; velocity average path (VAP) 114.99 μm/s and 64.04 μm/s; linearity (LIN) 0.53% and 0.44%; straightness (STR) 0.83% and 0.75%; wobble (WOB) 0.64% and 0.58%; lateral head displacement (ALH) amplitude 5.83 μm and 5.15 μm; beat cross frequency (BCF) 35.28 Hz and 24.65 Hz. The kinematics of fresh and frozen semen spermatozoa significantly affect the value of motility characteristics. The conclusion of the results of this study is that there is a decrease in the quality of fresh semen kinematics and frozen semen kinematics spermatozoa on the value of motility characteristics, namely on the speed of spermatozoa at the velocity curvilinear (VCL), velocity straight line (VSL), and velocity average path (VAP), linearity (LIN), straightness (STR), and wobble (WOB) and on the distance value of spermatozoa amplitude lateral head displacement (ALH) and beat cross frequency (BCF).
Co-Authors - Montesqrit Af&#039;ida Khofsoh Ahmad Dody Setiadi Arif Darmawan Ekayanti Mulyawati Kaiin Elly Roza ENDRI ENDRI Faizal, Noval Jumatul Harif Gusdinal Herbert Sipahutar Hilda Susanty Inggit Kresna Maharsih Jaswandi . Juwita Fitri, Rama Khalil Khalil Kusnadidi Subekti Linda Suhartati M Agus Setiadi Meida Nugrahalia Muhammad Hamzah Solim N Nurhasanah Ni Wayan Kurniani Karja Ramadhan, Rio Rizqan Rizqan Robi Amizar Rusfidra rusfidra Rusli, Ridho Kurniawan Sabrina, S Yulia Yelita Yulia Yellita Zurmiati