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All Journal Majalah Kedokteran Bandung Damianus Journal of Medicine Universa Medicina Dinamisia: Jurnal Pengabdian Kepada Masyarakat Kompetensi Mitramas Jurnal Pengabdian dan Pemberdayaan Masyarakat Mitramas Jurnal Pengabdian dan Pemberdayaan Masyarakat
Hanna Yolanda
Department Of Parasitology, School Of Medicine, Atma Jaya Catholic University Of Indonesia
Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Universa Medicina

 1 
Uncontrolled blood sugar tends to increase prevalence of dermatomycosis in diabetic type 2 patients Surja, Sem Samuel; Hermawan, Melyawati; Wijaya, Meiliyana; Pramanta, Pramanta; Yolanda, Hanna
Universa Medicina Vol 37, No 3 (2018)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.675 KB) | DOI: 10.18051/UnivMed.2018.v37.188-194

Abstract

Background The prevalence of type 2 diabetes mellitus (DM) is increasing. Diabetic patients have a higher risk of getting dermatomycosis. Dermatomycoses, although a common health problem amongst DM, is often misdiagnosed and consequently undertreated. Studies on the association between dermatomycosis and type 2 diabetes are lacking, especially in Indonesia. Therefore, the aim of this study was to determine the prevalence, etiology, and association of dermatomycosis with diabetic control of type 2 DM. MethodsA cross-sectional study was performed involving 87 subjects with type 2 DM. Demographic and clinical data, including age, sex, and blood glucose level, were collected. If a dermatomycosis lesion was found, a specimen would be taken for identification. Determination of serum glucose level was conducted using Roche c111 analyzer®. Statistical analysis was performed with the chi-square test and Kolmogorov-Smirnov two-independent sample test.ResultsSeventeen (19.55%) subjects had dermatomycosis. The predominant age group affected was 51 - 60 years (42.4%). The number of clinically apparent dermatomycosis was greater in the uncontrolled than in the controlled blood sugar group, but the difference was statistically not significant (p > 0.05). The lesions were mostly found on the nails (74%) and the most common etiology was candida (50%) followed by dermatophyte (25%) and non-dermatophyte molds (25%). ConclusionUncontrolled blood sugar tends to increase the risk of dermatomycosis in type 2 DM patients. Fungal skin infections are common in type-2 DM patients, especially in those with poor glycemic control.
Nepenthes rafflesiana pitcher liquid has antifungal activity against Candida spp. Hanna Yolanda; Ingrid M. Makahinda; Maureen Aprilia; Nikki Sanjaya; Harry Gunawan; Rita Dewi
Universa Medicina Vol. 33 No. 2 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.83-90

Abstract

BACKGROUNDTo develop new effective antifungals, it is essential to search for antifungal compounds from plants such as Nepenthes spp., which have their greatest diversity in Indonesia. Since chitin-induced liquid (CIL) from Nepenthes khasiana pitchers has antifungal activity, due to their naphthoquinone content, this study aimed to evaluate antifungal activity of Nepenthes rafflesiana pitcher liquids on Candida spp.METHODSCollected pitcher liquids were of 3 types: non-induced liquid (NIL), prey-induced liquid (PIL), and chitin-induced liquid (CIL). Non-induced liquid (NIL) was collected from fresh naturally opened pitchers, PIL from opened pitchers after 3 hours of induction with Zophobas morio larvae, and CIL from closed pitchers after 5 days of chitin solution injection. The antifungal activity of the liquids against C. albicans, C. glabrata, C. krusei, and C. tropicalis were detected by disc diffusion and macrodilution methods.RESULTSInhibition zone diameters of NIL, PIL, and CIL against C. albicans were 35.00 (35.00 – 39.33) mm, 26.33 (23.00 – 40.00) mm, and 30.00 ( 28.00 – 32.00) mm, respectively, while for C. glabrata the zone diameters were 22.22 ± 3.66 mm, 29.89 ± 2.79 mm, and 28.89 ± 1.17 mm, respectively. No inhibition zones were found for NIL, PIL, and CIL against C. krusei and C. tropicalis. At concentrations of 80%, almost all samples showed visually apparent inhibition of fungal growth.CONCLUSIONThe pitcher liquid of N. rafflesiana has antifungal properties, presumably due to the presence of many potentially active substances, such as naphthoquinones, as has been proven in other studies.
Hexane neem leaf extract more potent than ethanol extract against Aspergillus flavus Jenny Hidayat; Ay Ly Margaret; Hanna Yolanda; Lies K. Wibisono
Universa Medicina Vol. 33 No. 3 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.171-178

Abstract

BACKGROUND Aspergillus flavus is one of the causes of aspergillosis, with a high virulence and resistance to standard antifungals, resulting in a high mortality rate. Medicinal plants are increasingly used as they are relatively safer with minimal side effects. Previously we found that the ethanol extract of neem (Azadirachta indica A Juss) leaves inhibits A. flavus growth in vitro. However, most chemical compounds with antifungal effect are nonpolar. The purpose of this research was to compare the antifungal effect of neem leaves extracted in a nonpolar solvent to that of leaves extracted in a polar solvent. METHODS An in vitro experimental research was conducted between October 2013 and January 2014. Neem leaves were extracted in ethanol or hexane at various concentrations. A macrodilution test with 48-hour incubation time was done in triplicate on 8 groups of samples. These comprised the neem leaf ethanol extract (NLEE) at 0.5, 1.0, and 2.0 g/dL, neem leaf hexane extract (NLHE) at 0.5, 1.0, and 2.0 g/dL, positive control, and negative control groups. Fungal growth was detected on Sabouroud dextrose agar. Statistical analysis used Chi square and Fisher’s exact test. RESULTS NLHE had a higher, but statistically non-significant, inhibitory effect on A. flavus than NLEE (p=0.996). At higher concentrations, the antifungal effect of NLHE is better than that of NLEE. CONCLUSION There is no significant difference in in-vitro inhibitory effectivity on A. flavus of neem leaves between extracts in polar and nonpolar solvents.
Antifungal activity of neem leaf ethanol extract on Aspergillus flavus Ayly Margaret; Hanna Yolanda; Wibisono Lies K
Universa Medicina Vol. 32 No. 2 (2013)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2013.v32.80-85

Abstract

BACKGROUNDAspergillosis is an infectious disease due to inhalation of Aspergillus spores.Drug resistance to aspergillosis has been reported since 14 years ago. Therefore,alternative treatment using herbals began to attract the attention of the medicalprofession and the community, because herbals are relatively safe and haveminimal side effects. Neem (Azadirachta indica) is an herb with broad spectrumbiological activity, but studies on the antifungal activity of neem leaves arelacking. The objective of this study was to evaluate the activity of neem leaveson Aspergillus flavus (A. flavus).METHODSThis was a laboratory experimental study using A. flavus samples obtained frombronchial washing specimens of inpatients. Neem leaf extract in ethanol (NLE)was prepared in various concentrations. Aspergillus. flavus antifungal activityof NLE were assessed by disc diffusion (using NLE at 1.0; 0.75; 0.50; 0.25 g/dL)and macrodilution (using NLE at 1.0; 0.75; 0.5; 0.25; 0.125 g/dL). Ketoconazole0.1 g/dL was used as antifungal positive control. Disc diffusion and macrodilutionwere conducted using Sabouraud dextrose agar and broth, to measure inhibitionzone diameter and minimal inhibitory concentration (MIC), respectively. Statisticalanalysis was performed using Mann-Whitney test from SPSS 15.RESULTSThere were significant differences in inhibition zone diameter (p = 0.034) betweenA. flavus samples exposed to NLE (1 g/dL) and the control group exposed toketoconazole (0.1 g/dL). The MIC of NLE against A. flavus was 0.5 g/dL.CONCLUSIONSNeem leaf ethanol extract has antifungal activity against A.flavus in vitro.
Co-Authors Ay Ly Margaret Ayly Margaret Felicia Anggraini, Felicia Harry Gunawan Hengestu, Anastasia Hermawan, Melyawati Ingrid M. Makahinda Jenny Hidayat Jeremiah Mai Nugraha Joprang, Freggy Spicano Kaisar, Maria Mardalena Martini Kristin, Helen Lies K. Wibisono Linda Suryakusuma Margareta, Revi Maureen Aprilia Muhyidin, Muhammad Fadli Natasha Gabby Ratimanjari Nikki Sanjaya Olivia, Vera Pramanta, Pramanta Rita Dewi Sem Samuel Surja Sinaga, Warisman Wibisono Lies K Wijaya, Meiliyana Yanti Mulyana