Sem Samuel Surja
Department Of Parasitology, Faculty Of Medicine And Health Sciences, Universitas Katolik Indonesia Atma Jaya, Jakarta, Indonesia

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Cafein and Infertility on Women Fedriani, Jessica; Surja, Sem Samuel
The Journal of The Indonesian Medical Students Association Vol. I No. 01 January - June 2010
Publisher : The Journal of The Indonesian Medical Students Association

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Abstract

Caffeine is a natural compund in many food and, such as coffee, tea, cocoa, and others. Indonesia is classified as one of the country which consume high number of caffeine. Eventhough caffeine can stimulate central nervous system, it may also bring undesireable effect such as infertility. Through this paper, we would like to review the effect of caffeine on females fertility and the safety limit of caffeine for daily consumption on women. This paper is made with study literature method. There are several studies which indicate that there is a strong relationship between caffeine consumption and infertility. The safety limit of the amount of caffeine that may affect fertility requires more research. Daily consumption of a cup of non-decaffeinated coffee of more showed a significant impact on fertility. There is greater risk of infertility at women who consume the greater amount of caffeine. Key words: caffeine, infertility
Chocolates Effect on Cardiovascular System Surja, Sem Samuel; Krisanti, Stefani; Ariwibowo, Hendrawan
The Journal of The Indonesian Medical Students Association Vol. I No. 01 January - June 2010
Publisher : The Journal of The Indonesian Medical Students Association

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Abstract

Chocolate is one of the most common foods which many people consume. Chocolate has many effects to human health, one of which is for cardiovascular system. Some rumors say that chocolate has deteriorating effect for cardiovascular system because of its high fat content. On the other hand, the levels of antioxidants in chocolate said to have a positive effect on cardiovascular system. This paper discusses more about the cardiovascular effects of chocolate. Various literatures starting from year 2003 to 2008 related to the topic is collected to support the writing of this paper. From the results of this literature review, it was found that consumption of chocolate can provide short-term improvement on endothelial function and reduction in systolic and diastolic blood pressure. Other effects such as effects on fat profiles and pulse are still controversial. Underlying component of chocolate that contribute to these effects is flavanoid. Another component of chocolate feared to have deteriorating effect is stearic acid, that may increase blood cholesterol, but it is still not proven in various studies. Author concludes that chocolate has positive short-term effects for cardiovascular system. But because of the limitations of the various studies, long-term effects of chocolate consumption are still not known with certainty. Type of chocolate that was considered as the most useful for cardiovascular system is high-flavanoid content dark chocolate. Key words: chocolate, cardiovascular system, flavanoids, endothelial function, blood pressure
Uncontrolled blood sugar tends to increase prevalence of dermatomycosis in diabetic type 2 patients Surja, Sem Samuel; Hermawan, Melyawati; Wijaya, Meiliyana; Pramanta, Pramanta; Yolanda, Hanna
Universa Medicina Vol 37, No 3 (2018)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.675 KB) | DOI: 10.18051/UnivMed.2018.v37.188-194

Abstract

Background The prevalence of type 2 diabetes mellitus (DM) is increasing. Diabetic patients have a higher risk of getting dermatomycosis. Dermatomycoses, although a common health problem amongst DM, is often misdiagnosed and consequently undertreated. Studies on the association between dermatomycosis and type 2 diabetes are lacking, especially in Indonesia. Therefore, the aim of this study was to determine the prevalence, etiology, and association of dermatomycosis with diabetic control of type 2 DM. MethodsA cross-sectional study was performed involving 87 subjects with type 2 DM. Demographic and clinical data, including age, sex, and blood glucose level, were collected. If a dermatomycosis lesion was found, a specimen would be taken for identification. Determination of serum glucose level was conducted using Roche c111 analyzer®. Statistical analysis was performed with the chi-square test and Kolmogorov-Smirnov two-independent sample test.ResultsSeventeen (19.55%) subjects had dermatomycosis. The predominant age group affected was 51 - 60 years (42.4%). The number of clinically apparent dermatomycosis was greater in the uncontrolled than in the controlled blood sugar group, but the difference was statistically not significant (p > 0.05). The lesions were mostly found on the nails (74%) and the most common etiology was candida (50%) followed by dermatophyte (25%) and non-dermatophyte molds (25%). ConclusionUncontrolled blood sugar tends to increase the risk of dermatomycosis in type 2 DM patients. Fungal skin infections are common in type-2 DM patients, especially in those with poor glycemic control.
Preservation in Paraffin Oil: Alternative for Fungi Preservation in Simple Laboratory Concept Sem Samuel Surja; Gabi Vania Sally; Favian Sergius Ruby Hartoyo; Sandy Vitria Kurniawan; Yanto Budiman
Althea Medical Journal Vol 7, No 3 (2020)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v7n3.1975

Abstract

Background: A good collection of fungi culture can be maintained with good preservation methods. With appropriate methods, preservation can be performed in low-budget laboratories, such as with periodic subculture and preservation in either sterile water or in paraffin oil. The aim of this study was to assess the viability, morphology, contamination, and antifungal susceptibility profile of fungi preserved in sterile water or paraffin oil compared to a periodic subculture.Methods: This study was an experimental study using Aspergillus sp., Trichophyton mentagrophytes and Candida albicans. The fungi were preserved by a periodic subculture or in sterile water or in paraffin oil for six months. A comparison of viability, morphology, contamination, and antifungal susceptibility profile between methods were made before and after six months of study.Results: The viability of all the fungi was maintained in both periodic subculture and preservation with paraffin oil. However, the T. mentagrophytes was not viable in preservation using sterile water. All methods could maintain the morphology without contamination. There were reductions in the inhibition zone against antifungal drug in preservation with sterile water and paraffin oil.Conclusion: Preservation in paraffin oil is superior to preservation in sterile water in terms of maintaining fungal viability. This method is suitable for the use in a simple laboratory, albeit considerations should be given to the reduction of inhibition zone.
ANALISIS METODE PRESERVASI SUBKULTUR BERKALA DAN DENGAN AIR STERIL UNTUK JAMUR Aspergillus sp. DAN Candida albicans Gabi Vania Sally; Sandy Vitria Kurniawan; Sem Samuel Surja
Bahasa Indonesia Vol 19 No 2 (2020): Damianus : Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v19i2.1193

Abstract

Introduction: A good and proper preservation can maintain all collection of culture. Periodic subculture preservation is an old technique that is less able to guarantee the genetic characteristic for a long time. Sterile water is an alternative method in various studies to ensure the genetic characteristic for a long time and can be done in a simple laboratory concept. This study aimed to examine the method of periodic subculture and with sterile water Methods: This research was a descriptive study with an experimental laboratory design on Aspergillus sp. and Candida albicans. This study examined at periodic subculture methods and with sterile water in maintaining viability, level of contamination, morphology, and antifungal resistance after six months Results: The fungus had lived after being preserved with periodic subcultures without contamination, morphological changes, and resistance. Aspergillus sp. and Candida albicans also lived after being preserved with sterile water for six months with the same morphology and without contamination. The zone of inhibition of Aspergillus sp. had decreased but in Candida albicans remains the same. Conclusion: Preservation with periodic subculture and with sterile water can be applied to Aspergillus sp. and Candida albicans.
The harada-mori technique: Revisited Agnes Immanuela Toemon; Handinata Indrawan; Sem Samuel Surja
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 13, No 2, (2022)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol13.Iss2.art11

Abstract

More sensitive diagnostic methods for detecting worms’ eggs are needed in areas with low soil-transmitted helminth (STH) prevalence. The Harada-Mori technique was first introduced by Mr. Harada and Mr. Mori. This technique is easy, simple, and does not require sophisticated equipment. The Harada-Mori technique is based on larvae tropism nature in the water to concentrate the larvae of hookworms and Strongyloides stercoralis. Since its first application in 1955, this technique has undergone several modifications. The Harada-Mori could complement the Kato-Katz technique for faeces examination in areas with low STH infection intensity.
Anticandidal Activity of Lactoferrin, Apolactoferrin, and Oligosaccharides on Mueller-Hinton and Sabouraud Dextrose Agar against Fluconazole Resistant-Candida Albicans Maureen Miracle Stella; Sem Samuel Surja; Zita Arieselia
Althea Medical Journal Vol 9, No 3 (2022)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v9n3.2408

Abstract

Background: The demand for novel, useful, potential, and safe antifungal drugs and rapid fungal susceptibility test methods due to antifungal resistance and high prevalence of Candida albicans infection are continuing. Therefore, this study aimed to assess and compare the antifungal activity of lactoferrin, apolactoferrin, and oligosaccharides isolated from human, bovine, goat, and formula milk against C. albicans on Mueller-Hinton agar supplemented with 2% glucose and 5 µg/mL methylene blue and sabouraud dextrose agar.Methods: Lactoferrin, apolactoferrin, and oligosaccharides were extracted from human, bovine, goat, and formula milk. Lactoferrin was identified using the Bradford test and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Oligosaccharides were identified using Thin-Layer Chromatography. The antifungal activity of lactoferrin, apolactoferrin, and oligosaccharides against fluconazole-resistant Candida albicans ATCC 10231 was determined and compared using the disk diffusion method on Mueller-Hinton agar and sabouraud dextrose agar. The inhibition zone formed around the disk was observed after 24 hours of incubation.Results: Lactoferrin showed an inhibition zone on sabouraud dextrose agar against C. albicans, but not on Mueller-Hinton agar. Meanwhile, apolactoferrin and oligosaccharides showed no antifungal activity on both agar media.Conclusions: Different agar media in the diffusion disk test can give different results even though using the same test method and substance. These results could shed light and become the useful references on why some potential antifungals could yield a different results in in-vitro studies, in-vivo studies, or clinical trials.
Germ Tube Induction Test Comparing Total of Six Liquid and Three Solid Media in Candida albicans Ruby, Rivaldi; Arifin, Erlangga Saputra; Kurniawan, Sandy Vitria; Surja, Sem Samuel
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 1 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i1.34097

Abstract

Invasive candidiasis (IC) has a high mortality rate of 70%, thus diagnosis should be established without delay. Given its fast result, serological test such as β-d-glucan (BDG) test is one alternative diagnosis modalities. However, it lacks specificity. Candida albicans germ tube antibody (CAGTA) test is an alternative serological test which has a high sensitivity of 76.2% and specificity of 80.3%.  Manufacturing CAGTA serological test requires provision of specific germ tube antigen. In this study, various culture media were tested to find the best media for germ tube induction. This study was an experimental in vitro study. The number and length of the germ tube were recorded in two- and three-hour incubation periods. A total of six samples containing one C. albicans ATCC 90028, four C. albicans wild type strains, and one C. krusei wild type strain were used. Nine media were tested to induce germ   tube formation: human and sheep serum, fetal bovine serum, mueller hinton agar and broth, tryptic soy agar and broth, brain heart infusion agar and broth. At both incubation periods, the medium with the highest number of germ tube was human serum (p=0.001 and p=0). The longest germ tube was found in sheep serum at two-hour incubation period (p=0.005). Mueller hinton broth (MHB) showed comparable results with human and sheep serum (p>0.05). Human serum is a superior inducer of morphogenesis. However, the use of MHB is recommended in this study, since provision of fresh human and sheep serum on a regular basis is impractical.
Tracing COVID-19 cluster during offline learning in the new era of pandemic Kaisar, Maria Mardalena Martini; Kristin, Helen; Widowati, Tria Asri; Rachel, Clarissa; Hengestu, Anastasia; Surja, Sem Samuel; Ali, Soegianto
Journal of Community Service and Empowerment Vol. 5 No. 1 (2024): April
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v5i1.27945

Abstract

Since January 2022, offline learning in the COVID-19 pandemic era has been going on for nearly a year. The government is attempting to revive the education sector, including universities. Nonetheless, this policy may result in the formation of a new SARS-CoV-2 infection cluster on campuses. One of the prevention measurements is conducting COVID-19 examinations. To support this effort, the infectious disease research team at Atma Jaya Catholic University of Indonesia (AJCUI) examined COVID-19 infection by saliva specimens using RT-qPCR method, along with the completion of related questionnaire and epidemiology investigation form. Throughout the first week of December 2022, community service was conducted at the Pluit campus of AJCUI with 749 participants (98.9% students). According to RT-qPCR results, only 12 participants (1.6%) were infected with SARS-CoV-2 virus. Most participants demonstrated positive compliance with health protocols, particularly, PeduliLindungi application scanning, hand washing, and mask usage. The implementation of health protocol resulted in a low positive rate compared to North Jakarta's statistics for the same week, preventing the formation of new COVID-19 clusters. The activity effectively controlled the spread of SARS-CoV-2 in AJCUI environment, continuing the postponed 2030 agenda of the third Sustainable Development Goal to ensure good health and well-being.
Screening and Education of Intestinal Protozoa Infection in Elementary School-Age Children, Penjaringan District, North Jakarta: Skrining dan Edukasi Tentang Infeksi Protozoa Usus pada Anak Usia Sekolah Dasar, Kecamatan Penjaringan, Jakarta Utara Maria Mardalena Martini Kaisar; Muhammad Fadli Muhyidin; Felicia Anggraini; Meiliyana Wijaya; Hanna Yolanda; Sem Samuel Surja; Helen Kristin; Anastasia Hengestu; Freggy Spicano Joprang
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 5 (2024): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v8i5.21596

Abstract

Urbanization in Jakarta has led to the development of slum settlements characterized by poor sanitation and limited access to clean water. This environment significantly increases the risk of intestinal protozoan parasitic infections (IPPIs), which are known to cause diarrhea and adversely affect the growth and development of children—key factors in the success of Indonesia's Emas 2045 initiative. To address this, the community service team from FKIK UNIKA Atma Jaya conducted a free fecal examination program for students at a public school in the Penjaringan District, a slum area in North Jakarta. The program included several phases: preparation, education, microscopic examination, and evaluation, involving both the school and the students' parents or guardians. Diagnostic methods utilized were direct examination and a modified Ziehl-Neelsen (ZN) staining technique. Out of 223 participants, 16.6% were found to be infected with IPPIs. The analysis revealed no significant correlation between infection rates and grade levels (p=0.106). This initiative is expected to enhance awareness among parents and students about IPPIs, leading to improved personal hygiene and sanitation practices. It also provides valuable data to authorities for assessing and formulating preventive policies against IPPIs