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All Journal The Journal of Pure and Applied Chemistry Research Journal of Tropical Crop Science Agroekoteknologi Tropika Lembab Dinasti International Journal of Education Management and Social Science Indonesian Journal for Social Responsibility Masker Medika Journal of Informatics and Communication Technology (JICT) Jurnal Pengabdian Ilung (Inovasi Lahan Basah Unggul) An-Nafis: Jurnal Ilmiah Keislaman dan Kemasyarakatan
Yudiansyah Yudiansyah
Department of Chemistry Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganeca 10 Bandung 40132
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Journal : Journal of Tropical Crop Science

 1 
Biodiversity Assessment of Foxtail Millet (Setaria italica L.) Genotypes Based on RAPD Marker ARDIE, Sintho Wahyuning; Khumaida, Nurul; Fauziah, Nurul; Yudiansyah, Yudiansyah
Journal of Tropical Crop Science Vol 4 No 1 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1155.767 KB) | DOI: 10.29244/jtcs.4.1.21-25

Abstract

Foxtail millet (Setaria italica L.) is an important crop in areas where harsh environmental conditions limit crop productivity, including in high salinity and drought prone areas. In Indonesia millet is cultivated in certain areas; however, superior varieties are less developed in the country. The objective of this study was to analyze the genetic diversity among foxtail genotypes using RAPD markers. Genomic DNA of ten foxtail millet genotypes was amplified using 26 random primers through RAPD analysis. Of these primers, 22 produced reproducible amplicons and were polymorphic among the 10 foxtail millet genotypes. The number of polymorphic markers for each primer varied from 1 (primer E15) to 14 (primer M17). The amplified product size ranged from 120 to 2500 base pairs (bp). A dendrogram constructed based on the UPGMA clustering method put all genotypes in 5 distinct groups at 0.64 coefficient level. Diverse genotypes identified in this study can be used as potential parents in an efficient crop improvement program.
Modification of The Spectrophotometric Analysis Protocol by Sims D. and Gamon to Analyze Leaf Pigment Content using Green Spinach (Amaranthus hybridus) as a Model Plant Yudiansyah, Yudiansyah; Ismiyanti, Ismiyanti; Aziz, Sandra Arifin
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.147-154

Abstract

The analysis of pigment content in plants, particularly chlorophyll a, chlorophyll b, anthocyanin, and carotenoids, is crucial to assess their physiological performance. The spectrophotometry method by Sims and Gamon offers practicality and reliable results. This research established several variables to achieve more precise results. Green spinach leaf samples were used as a model to analyze their pigment content, determining the minimum sample area or weight, the maximum limit that does not violate Beer’s law, and the temperature during analysis. The limit of quantitation (LoQ) is the smallest quantity of analytes in a sample that still meets the criteria for accuracy and precision in testing. The research results show that using a leaf punch with a diameter of 9 mm approaches the LoQ value. To avoid measurements nearing the LoQ limit, it is advisable to use leaf punch diameters of 10- or 12-mm. Leaf punches of 22 mm are still safe to use, as there has been no deviationfrom Beer’s law. Using unchilled acetone at 27°C did not show significant differences compared to using cold acetone at 7°C for the content of chlorophyll a, total chlorophyll, and carotenoids. However, for chlorophyll b and anthocyanin, despite significant differences, the chlorophyll content measured at room temperature was more practical and relatively higher compared to cold acetone, thus disproving concerns about pigment damage.
Co-Authors Akhid Yulianto Ali Sadikin Andi Muhammad Baso Andryansyah, Andryansyah Aulia, zasmina Dahniar Dahniar Dalimunthe, Fahmi Roy Deana Wahyuningrum Eko Nursalim Evi Marpiah Fitriani Fitriani Ika Guslanda Bustam Ismiyanti, Ismiyanti Lia Hafiza Lucy Arianie Niswah, Nailah Khairun Nora Idiawati Nurul Fauziah Nurul Khumaida Rachman, Mohammad Yudi Rahmah, Githa Desyana Nur Rizka Rachmawati Sandra Arifin Aziz Sintho Wahyuning Ardie Toyibulah, Yoga Tukhas Shilul Imaroh Utin Dewi Pebriyana Vivi Monita Wahyuningrum, Reni Dyah