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All Journal HAYATI Journal of Biosciences Journal of Tropical Soils IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) JPSriwijaya Jurnal Penelitian Sains Seminar Nasional Lahan Suboptimal Sriwijaya Bioscientia BIOVALENTIA: Biological Research Journal
Bambang Yudono
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Sriwijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Other

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Eksplorasi Bakteri Hidrokarbonoklastik dari Rhizosfer di Lahan Tambang Minyak Rakyat, Kecamatan Babat Toman, Sumatera Selatan Dwi Hardestyariki; Bambang Yudono; Munawar Munawar
Jurnal Penelitian Sains Vol 16, No 3 (2013)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.983 KB) | DOI: 10.56064/jps.v16i3.65

Abstract

This study aims to obtain rhizosphere bacteria as a potential agent of bioremediation. The method of sampling the plant rhizosphere by purposive sampling. Soil samples were taken from around the rhizos-phere of plants that live in soil contaminated oil. Bacteria were isolated from the rhizosphere soil samples, then they were selected into steps : performed purification, selection I and selection II. The among bacteria are tested the synergism, then characterized and identified it’s genus. The results showed that there were as many as 34 rhizosphere bacterial isolates were collected from three different sampling locations. Identification of bacteria that have potential as a bioremediation agent. Each bacterial isolate was identified as Sporosarcina sp. A.4.10, Proteus sp. C.6.7, Actinobacillus sp. A.6.3, and Flavobacterium sp. A.5.8.
The Analysis of Manganese (Mn) in Waste Water Treatment (IPAL) of Coal Mine of PT Bukit Asam Indonesia Getari Kasmiarti; Rona Ayu Sakinah; Bambang Yudono
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 6, No 2 (2021): June 2021
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v6.i2.53

Abstract

Wastewater from the coal mining process containing heavy metal manganese is bad for water body and health of living things around it.  The South Sumatra Governor Regulation states that the content of iron (Fe) and manganese (Mn) must contain a maximum total of 7 mg/L and 4 mg/L before being released into the environment. This study aimed to verify the total dissolved Mn analysis method. Thirty-four (34) samples from the wastewater treatment plant pond were digested and measured by the Atomic Absorption Spectrophotometer (AAS) at a wavelength of 279.5 nm.  The analyzes performed were the precision, sensitivity, linearity, LOD detection limits and LOQ quantization limits. The measurement results showed the parameter value for a precision of 1.562%; linearity (coefficient of determination) standard curve 0.9939; sensitivity 0.0375; LOD 0.4287 g/mL; and LOQ 1.4291  g/mL. In conclusion, the measurement data for the total dissolved manganese met the quality standards for wastewater stated in the Regulation of the Governor of South Sumatra No.8 of 2012 by applying the predetermined method.
The Analysis of Total Dissolved Iron from Mud Sedimentation Ponds Wastewater in PT Bukit Asam (Persero) Tbk Tanjung Enim Using Individual Control Diagram Getari Kasmiarti; Bambang Yudono
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 5, No 2 (2020): June 2020
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v5.i2.29

Abstract

The tests have been carried out on the iron content of wastewater in the inlet and outlet of the mud sedimentation ponds PT Bukit Asam (Persero) Tbk Tanjung Enim, South Sumatra Province. The iron concentration in mining waste water that does not comply with government standards of 7.0000 mg / L can pollute the environment. The measurement of iron concentration was carried out by Atomic Absorption Spectrophotometer (AAS) method in accordance with SNI 06-6989.4: 2004. Analysis of iron concentration using calibration curve methods and individual control diagrams. The results of data analysis showed that the iron content of the inlet and outlet MSP (Mud Sedimentation Ponds) wastewater was still under the standard set with an average value of 1.4488 mg/L inlet and outlet 0.4061 mg/L. Based on the results of the study the concentration of iron meets the established quality standards.
Bioremediation of Spent Bleaching Earth (SBE) Waste Using Lipolytic Fungi Riezkatama Menangcaye; Adipati Napoleon; Bambang Yudono
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 3, No 3 (2018): October 2018
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v3.i3.59

Abstract

The purpose of this research was to obtain the effectiveness level of bioremediation process of oil residue on SBE waste by using the isolates of lipolytic fungus Aspergilus fumigatus, Cylindrocladium sp and Fumago sp. This research was conducted by using completely randomized factorial design with 3 factors of treatment which consist of nutrient ratio (N:P:K) that were divided into three levels, namely n1=A(7:1.5:0.5), n2=B(14:3:1), n3=C(21:6:1.5). The humidity were divided into 3 levels treatment i.e. k1 (90%), k2 (80%), k3 (70%) and time that were divided into four levels, namely w1 (week 1), w2 (week 2), w3 (week 3), and w4 (week 4) with fixed pH i.e. pH 6, so that were obtained 36 combination of treatment and for each treatment combination repeated 3 times. Sampling of SBE waste was conducted in one cooking oil factory in South Sumatera using randomized sampling method as much as 24.000 g. The results showed that 80% humidity and nutrient A as the best treatment for lipolytic fungus consortium performance to degrade the SBE waste with the average percentage of oil degradation as much as 74.83%. 80% humidity and nutrient A was an effective interaction in affecting the percentage of oil degradation.
PIROLISIS LIMBAH PLASTIK MENJADI BAHAN BAKAR MINYAK MENGGUNAKAN PEMANAS INDUKSI DI DESA BINAAN PULAU SEMAMBU, INDERALAYA, KAB. OGAN ILIR Bambang Yudono
Jurnal Pengabdian Sriwijaya Vol 9, No 1 (2021)
Publisher : Lembaga Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37061/jps.v9i1.17445

Abstract

Kegiatan PkM pengolahan sampah plastik menjadi bahan bakar minyak melalui proses pirolisis sampah plastik menggunakan pemanas induksi telah dilakukan di desa Pulau Semambu. Kegiatan diikuti oleh ibu-ibu PKK dan Pemuda Karang Taruna desa Pulau Semambu. Kegiatan ini merupakan hasil desiminasi penelitian yang telah dilakukan di Jurusan Kimia FMIPA Universitas Sriwijaya. Sampel yang digunakan adalah jenis Low Density Polyethylene, Polypropylene, dan Polyethylene Terephthalate (LDPE, PP, dan PET) yang diperoleh dari limbah domestik. Masing-masing plastik memperoleh distilat sebanyak 190,56 mL atau sebesar 38,11% pada plastik LDPE; sebanyak 327,39 mL atau sebesar 65,49% pada plastik PP; sebanyak 43,26 mL atau sebesar 8,65% pada plastik PET; dan sebanyak 160 mL atau sebesar 32% pada residu LDPE pada suhu 40-130oC, Sedangkan pada distilat residu LDPE pada temperatur  160-190oC berada pada fraksi kerosene (minyak tanah), dengan jumlah hidrokarbon C11-C13 menghasilkan distilat sebanyak 84 ml atau sebesar 16,8%.  Densitas rata-rata minyak pirolisis plastik LDPE; PP; dan PET berturut-turut sebesar 0,738 gr/mL; 0,740 gr/mL; dan 0,764 gr/mL. Hasil analisis GC menunjukkan bahwa minyak hasil pirolisis sampah plastik menggandung senyawa hidrokarbon yang sangat komplek.
PENGEMBANGAN SENTRAINDUSTRI GULA KELAPA DESA SRI MENANTI Bambang Yudono
Jurnal Pengabdian Sriwijaya Vol 7, No 3 (2019)
Publisher : Lembaga Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37061/jps.v7i3.10138

Abstract

Pengembangan industri gula kelapa dilakukan dengan melakukan dengan cara derivikasi produk menjadi  menjadi gula kelapa herbal (Jamu instan Beras Kencur, Kunyit Asam, Beras Kencur). Produk ini dapat meningkatkan nilai tambah yang cukup signifikan yaitu dari harga gula kelapa Rp 20.000,- per Kg menjadi Rp 100.000,- per Kg.  Pendekatan, metode dan teknik yang digunakan dalam kegiatan ini adalah PRA (Participatory Rural Appraisal). Partisipasi sepadan dengan arti peran serta, ikut serta, keterlibatan, atau proses belajar bersama saling memahami, menganalisis, merencanakan dan melakukan tindakan oleh sejumlah anggota masyarakat itu sendiri. Pengembangan masyarakat dan pembangunan desa akan dimulai dengan cara yang sederhana, yaitu suatu proses yang berangkat dari kemampuan, kemauan dan kebutuhan masyarakat dalam memenuhi penghidupan dan menyelenggarakan kehidupannya. Diharapkan kegiatan ini dapat dirasakan tumbuh dan dimiliki oleh masyarakat, sehingga kegiatan ini dapat berkembang secara berkelanjutan, dan kerjasama antara LPPM Universitas Sriwijaya dengan masyarakat Desa Mulyasari dan Desa Sri Menanti akan semakin berkembang dengan baik.  Hasil dari kegiatan ini bagi masyarakat adalah munculnya industri kecil Gula Kelapa Herbal (Beras Kencur, Jahe, dan Kunyit Asem), kegiatan ini memberikan pengetahuan, ketrampilan alternatif usaha baru bagi masyarakat desa Sri Menanti, kecamatan Tanjung Lago kabupaten Musi Banyuasin Sumatra Selatan, dan memberikan untuk meningkatkan pendapatan keluarga. Kegiatan ini juga akan memberikan nilai tambah pada koperasi himpunan wanita Transmigrasi (HW trans) di desa Sri Menanti. Produk jamu herbal (Jamu Beras Kencur, Kunyit Asam, Beras Kencur) dapat dijual di masyarakat dengan  harga Rp 100.000,- per Kg, meningkat harganya cukup signifikan dibandingkan dengan gula merah (gula kelapa) Rp 20.000,- per Kg. Hasil uji organoleptik skala 5 untuk jamu herbal terhadap rasa, warna, dan aroma masing-masing menunjukkan 3.7, 3.86, dan 3.7. Hasil uji tersebut menunjukkan bahwa produk tersebut tergolong disukai oleh masyarakat
Uji kemampuan konsorsium bakteri hidrokarbonoklastik sebagai agen bioremediasi Dwi Hardestyariki; Bambang Yudono
Sriwijaya Bioscientia Vol 1 No 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.935 KB) | DOI: 10.24233/sribios.1.1.2020.166

Abstract

Penelitian ini bertujuan untuk mencapatkan bakteri rhizosfer yang berpotensi sebagai agen bioremediasi. Pengambilan sampel pada rhizosfer tanaman digunakan metode purposive sampling. Sampel tanah diambil dari sekitar rhizosfer tanaman yang tumbuh di tanah yang terkontaminasi oleh minyak. Bakteri diisolasi dari sampel tanah di sekitar rhizosfer, yang kemudian dilakukan seleksi dengan langkah-langkah yaitu dilakukan pemurnian, seleksi tahap 1 dan seleksi tahap 2. Hasil dari penelitian ini didapatkan sebanyak 34 bakteri yang diisolasi dari 3 lokasi sampling yang berbeda. Dari keseluruhan bakteri diperoleh 4 isolat bakteri yang memiliki kemampuan baik dalam bioremediasi, yaitu  isolat A.4.10;  A.6.3 ;  C.6.7;   dan A.5.8. Kultur campur keempat isolat bakteri tersebut diketahui dapat dijadikan sebagai agen bioremediasi yang dapat dilihat dari peningkatan populasi bakteri, persentase degradasi TPH, dan peningkatan kadar CO2 dalam proses bioremediasi.
Karakteristik Virgin Coconut Oil (VCO) dan Protein Krim Santan yang Diektraksi dengan Metode Pancingan Parwiyanti, Parwiyanti; Lidiasari, Eka; Yudono, Bambang; Aprilia, Rantika; Debi Yora, Riska
Seminar Nasional Lahan Suboptimal Vol 10, No 1 (2022): Prosiding Seminar Nasional Lahan Suboptimal ke-10 “Revitalisasi Sumber Pangan N
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Parwiyanti P, Lidiasari E, Yudono B, Aprilia R, Yora RD. 2022. Characteristics virgin coconut oil (VCO) and protein of coconut milk cream extracted by fishing method. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-10 Tahun 2022, Palembang 27 Oktober 2022. pp. 337-345.  Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).The coconut product that has high added value as a raw material for cosmetics and medicine was Virgin Coconut Oil (VCO). The objective of this research was to determine effect of the concentration induced VCO and the speed of stirring on the VCO and protein characteristics of coconut milk cream. The experiment used a Factorial Completely Randomized Design with two factors. The first factor was VCO concentration consisting of 3 levels (5%, 7%, and 10%) and the second factor was stirring speed consisting of 3 levels (500, 700, and 900 rpm). The parameters were yield, moisture, free fatty acids, degree of clarity and peroxide number for VCO and yield, moisture, ash and protein content for protein. The results for VCO showed that the concentration of induced VCO had a significant effect on the yield, stirring speed had a significant effect on the value of free fatty acids, the interaction of the two treatment factors had a significant effect on the value of the degree of clarity. The best treatment for VCO was induced VCO concentration of 5% and stirring speed of 500 rpm. For protein: the interaction of the induced VCO and stirring speed  had significant effect on water and protein content. The best treatment for protein was obtained in the 10% induced VCO concentration and stirring speed of 900 rpm.
Kinetics Approach of Biodegradation of Petroleum Contaminated Soil by using Indigenous Isolated Bacteria Yudono, Bambang; Said, Muhammad; Sabaruddin, .; Napoleon, Adipati; Fanani, Zainal
JOURNAL OF TROPICAL SOILS Vol. 16 No. 1: January 2011
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2011.v16i1.33-38

Abstract

The bioremediation of petroleum contaminated soil was investigated using a microscale Landfarming. The Indigenousbacteria, Pseudomonas pseudoalcaligenes, Bacillus megaterium, and Xanthobacter autotrophicus were isolated from the contaminated sites Sungai Lilin Jambi Pertamina Ltd and used further in the bioremediation experiments. The biodegradation rates of petroleum contaminated soil in the presence of the isolated bacteria were studied by using the chemical kinetics approach. The reaction orders were studied by using the differential method and the reaction rate constants were studied by using the integral method. The results showed that the reaction orders were 1.0949, 1.3985, 0.8823, and the reaction rate constants were 0.0189, 0.0204, 0.0324 day-1, respectively. Considering the values of reaction orders and reaction rate constants, the biodegradation rate of contaminated soil by usingeach bacteria had significantly different value; Xanthobacter Autotrophicus bacteria could degrade the petroleumoil sludge fastest than the others.Keywords: Biodegradation, indigenous bacteria, kinetics, petroleum oil
Co-Authors A. Napoleon Adipati Napoleon ADIPATI NAPOLEON Adipati Napoleon Ady Mara, Ady Aliyenah Aliyenah Aprilia, Rantika Debi Yora, Riska Dwi Hardestyariki, Dwi Eka Lidiasari F. Oesman F. Oesman Fahri Arlio Nita Getari Kasmiarti Getari Kasmiarti Hary Wahyudi Hary Widjajanti Hasanudin Hasanudin Juswardi Juswardi MARYATI BUDI UTAMI MUHAMMAD SAID MUHAMMAD SAID Munawar Munawar Munawar Munawar Parwiyanti Parwiyanti, Parwiyanti Poedji Loekitowati H Riezkatama Menangcaye Rona Ayu Sakinah SABARUDDIN, . Sri Pertiwi E Sri Pertiwi E Suheyanto Suheryanto Sy. Ummi Kalsum Victor H S Victor H S Zainal Fanani Zainal Fanani