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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Ilmu Lingkungan Jurnal Lahan Suboptimal Sains Tanah IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains IJEMS (Indonesian Journal of Environmental Management and Sustainability) Molekul: Jurnal Ilmiah Kimia Journal of Tropical Marine Science Berkala Penelitian Hayati SRIWIJAYA JOURNAL OF ENVIRONMENT BIOVALENTIA: Biological Research Journal
Hary Widjajanti
Jurusan Biologi FMIPA, Universitas Sriwijaya, Sumatera Selatan, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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EFFECT OF LIME APPLICATION ON INDIGENOUS NITROGEN-FIXING BACTERIA IN TIDAL SOILS MANAGED FOR THAN 30 YEARS Ridwan, Rahmawati; Budianta, Dedik; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol. 3 No. 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1119.549 KB) | DOI: 10.24233/BIOV.3.2.2017.70

Abstract

The study was conducted to determine the effect of land typology and lime on the population of indigenous nitrogen-fixing bacteria and soil pH, and to analyze the effect of soil typology interaction and lime on the population of indigenous nitrogen-fixing bacteria and soil pH. Sampling of soil in tidal land of Mulya Sari and Banyu Urip Village of Banyuasin Regency, South Sumatera. Then the sample was studied at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Inderalaya. During January-May 2017. Based on the results of the study known the soil typology of the total population of nitrogen-fixing bacteria, the highest population of nitrogen inhibitor bacteria in the second week of soil typology D was 6.0x107 and the highest pH of 5.18 in typology B on week Third, the lime treatment that affected the population of indigenous nitrogen-fixing bacteria, obtained the population of bacteria as much as 9.3x105 in the second week and the highest soil pH 4.93 in the second week, while the results of the interaction between soil typology and lime treatment only affect the population of bacterial inhibitor Indigenous nitrogen, with bacterial population of 3.5x107 in soil typology D in the second week, but this interaction did not significantly affect soil pH, with the highest pH measurement of 5.33 on the soil typology D.Keywords: nitrogen-fixing bacteria, tidal land, lime treatment
POTENCY AND ACTIVITY OF SECONDARY METABOLITE OF Trichoderma harzianum AC1(b) J2 INHIBITOR GROWTH Colletotrichum capsici IPBCC 13.1098 Nurkayah, Nurkayah; Nurnawati, Elisa; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol. 5 No. 1 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.76 KB) | DOI: 10.24233/BIOV.5.1.2019.130

Abstract

Trichoderma harzianum is a fungus that can produce secondary metabolites which able to inhibit the growth of Colletotrichum capsici. a pathogenic fungus causing anthracnose in plants. the Aims of this research were to obtain and analyze the ability of secondary metabolites of Trichoderma harzianum AC1 (b) J2 isolated from Scleria poaeformis. to inhibit the growth of Colletotrichum capsici IPBCC13.1098. The secondary metabolite eluat of Trichoderma harzianum were tested for the antifungal activity toward Colletotrichum capsici IPBCC 13.1098. Fractionation was carried out by Column Chromatography and produced 31 eluat. The secondary metabolite eluat of Trichoderma harzianum with the highest inhibition zone diameter was eluat 1 with 8.4 mm in diameter. The secondary metabolite eluat with the highest value of inhibition zone was carried out by MIC test and thin layer chromatography (TLC). Based on the MIC results, the minimum inhibitory concentration of the secondary metabolite of Trichoderma harzianum to fungus Colletotrichum capsici was 250 ppm with inhibition zone diameter was 0.10 mm. The results of TLC showed orange spots on the TLC plate that indicated alkaloite compounds.
EXPLORATION OF ENDOPHYTIC FUNGI OF DRAGON SCALE’S FERN (Pyrrosia piloselloides (L.) M.G. Price) AS AN ANTIBACTERIAL SOURCES Asiandu, Angga Puja; Widjajanti, Hary; Nurnawati, Elisa
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.513 KB) | DOI: 10.24233/BIOV.5.2.2019.149

Abstract

Endophytic fungi are fungi which live inside the host plant tissue and have been undergone a horizontal gene transfer process. Endophytic fungi are able to synthesize the same bioactive compounds which synthesized by their host plants. The host plant used in this research was dragon’s scales fern (Pyrrosia piloselloides (L.) M.G. Price). Dragon’s scales fern produces various of bioactive compounds which used as antibacterial agents such as polyphenols. This research was aimed to obtain endophytic fungi isolates from trophophyll fronds and sporophyll fronds of dragon’s scales fern, to determine the antibacterial activity of the secondary metabolite extracts of endophytic fungi, to determine the Minimum Inhibitory Concentration (MIC), to determine the characteristics of the endophytic fungi isolates which potentially as antibacterial source. Based on the research, 13 endophytic fungi isolates were obtained from dragon’s scales fern fronds consist of 5 isolates from trophophyll fronds and 8 isolates from sporophyll fronds. The antibacterial activity test showed that the extract of secondary metabolites of the isolate DTP2 had the highest inhibition zone diameter against E.coli 14.82 ± 4.05 mm, DTP4 against S.aureus 8.80 ± 0.03 mm and DSP4 against S.dysentriae 10.15 ± 0.36 mm. MIC of ethyl acetate extracts of secondary metabolites of isolate DTP2 against E.coli was 125 µg/mL, DTP4 against S.aureus was 125 µg/mL and DSP4 against S.dysentriae was 31.25 µg/mL. The endophytic fungi isolate DTP2 identified as Aureobasidium melanogenum, DTP4 identified as Penicillium alliisativi and DSP4 identified as Aspergillus flocculosus.
ANTIOXIDANT ACTIVITY of THE SECONDARY METABOLITES PRODUCED by ENDOPHYTIC FUNGI ISOLATED from JERUJU (Acanthus ilicifolius L.) PLANT Putri, Amanda Rahmaniah; Salni, Salni; Widjajanti, Harry
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.374 KB) | DOI: 10.24233/BIOV.5.2.2019.153

Abstract

Twelve endophytic fungi were isolated from Jeruju plant. Three of which have the best antioxidant activity, namely Culvularia lunata DJS1, Diaporthe phaseolorum DJS2, and Colletotrichum siamense BJS4. The fungi can produce secondary metabolites that have the potential as antioxidant compounds. This study aims to isolate the antioxidant compound using a chromatography column and test the antioxidant activity using DPPH method. Isolation of secondary metabolites produced 40 eluates. The IC50 value of DJS111 and BJS42 eluates were 10.20 µg/ml and 20.89 µg/ml, which is an antioxidant compound with very strong activity. The IC50 value of DJS24 eluate was 54.45 µg/ml, which is strong antioxidant activity. DJS111 eluate has antioxidant activity that approaches to ascorbic acid as a standart antioxidant compound (7.76µg/ml). From TLC, DJS111 contains flavonoid compound and DJS24 and BJS42 contain terpenoid compound.
Skrining Bakteri Kitinolitik Antagonis Terhadap Pertumbuhan Jamur Akar Putih (Rigidoporus lignosus) dari Rizosfir Tanaman Karet Muharni Muharni; Hary Widjajanti
Jurnal Penelitian Sains Vol 14, No 1 (2011)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1610.825 KB) | DOI: 10.56064/jps.v14i1.128

Abstract

Skrining bakteri kitinolitik dari rizosfir tanaman karet dilakukan dengan tujuan untuk memperoleh isolat bakteri kitinolitik yang antagonis terhadap pertumbuhan jamur akar putih (Rigidoporus lignosus). Bakteri diisolasi dari Perkebunan Karet Sembawa Kab. Banyuasin, dan skrining dilakukan dengan menggunakan media agar kitin dan uji antagonis terhadap jamur patogen dilakukan dengan menggunakan metode Chernin et al., (1995). Hasil penelitian didapatkan dua isolat bakteri kitinolitik yang antagonis terhadap pertumbuhan jamur akar putih (Rigidoporus lignosus). Identifikasi kedua isolat antagonis jamur akar putih ini tergolong kedalam genus Bacillus yaitu Bacillus sp. dan Bacillus apiarius
Deteksi Beberapa Bakteri Patogen pada Berbagai Jenis Ikan Konsumsi yang Laku Dijual di Pasaran Palembang Munawar Munawar; H. Widjajanti H. Widjajanti; E. Patriono E. Patriono; Sarno Sarno; A. Wulandari A. Wulandari
Jurnal Penelitian Sains No 1 (1996)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3085.301 KB) | DOI: 10.56064/jps.v0i1.225

Abstract

Penelitian menunjukkan bahwa ikan-ikan konsumsi yang terdiri atas 13 jenis dari ketiga pasar tempat pengambilan sampel tidak mengandung Salmonella, tetapi ditemukan satu jenis ikan, yaitu ikan gabus yang diambil dari salah satu pasar tempat pengambilan sampel mengandung V. Cholerae. Sedangkan hasil perhitungan angka kuman dari semua jenis ikan menunjukkan masih di bawah batas maksimum yang ditentukan oleh Departemen Kesehatan Republik Indonesia. Antara angka kuman dengan keadaan morfologi ikan menunjukkan terdapat hubungan yaitu keadaan morfologi ikan semakin jelek, angka kuman semakin meningkat. 
Pengaruh MVA Gigaspora Margarita dan Bakteri Pelarut Fosfat Bacillus Megaterium Terhadap Tanaman Kedelai Hary Widjajanti
Jurnal Penelitian Sains No 10 (2001)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (825.534 KB) | DOI: 10.56064/jps.v0i10.355

Abstract

Mikoriza berperan dalam meningkatkan serapan unsur hara bagi tanaman terutama unsur P dan bakteri fosfat mampu melarutkan fosfat yang ada di dalam tanah sehingga menjadi tersedia bagi tanaman. Tujuan penelitian adalah untuk mengetahui pengaruh mikoriza vasikular arbuskular (MVA) Gigaspora margarita dan bakteri pelarut fosfat (BPF) Bacillus megaterium terhadap tanaman kedelai yang ditanam di tanah Ultisol. Penelitian dilakukan di kebun dan Laboratorium Mikrobiologi FMIPA Unsri dengan menggunakan rancangan acak lengkapdengan pola faktorial dengan faktor I adalah dosis inokulum MVA yang terdiri atas: tanpa inokulasi BPF, 5 ml suspensi BPF dan 10 ml suspensi BPF. Kombinasi perlakuan diulang tiga kali dan pengamatan diulang 2 (dua) kali yaitu: pada fase R5 dan pada fase produksi. Hasil penelitian menunjukkan bahwa fase R5 inokulasi ganda MVA dan bakteri pelarut fosfat meningkatkan persentase infeksi MVA, serapan P tanaman, dan berat kering tanaman. Pada akhir fase produksi inokulasi ganda MVA dan bakteri pelarut fosfat tidak berpengaruh terhadap berat kering biji per tanaman. Inokulasi ganda MVA dan bakteri pelarut fosfat meningkatkan kadar P tanah.
Pemanfaatan Rumput Fimbrisylis sp. dalam Proses Bioremediasi Tanah pada Berbagai Konsentrasi Limbah Minyak Bumi Sri Pertiwi E; Hary Widjajanti; Bambang Yudono; Hary Wahyudi
Jurnal Penelitian Sains Vol 14, No 1 (2011)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v14i1.129

Abstract

Penelitian pemanfaatan rumput Fimbristylis sp dalam proses bioremediasi bertujuan untuk mengamati pengaruh pemanfaatan Fimbristylis sp. terhadap jumlah populasi bakteri dan persentase penurunan TPH pada proses bioremediasi. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) berpola faktorial yang terdiri dari 2 faktor. Faktor 1: kosentrasi limbah minyak bumi yang terdiri dari 5 taraf: C0:0%, C1:5% , C2:10%, C3:15% dan C4: 20% Faktor 2 Penggunaan rumput Fimbrisylis sp yang terdiri dari 2 taraf : R0 : Tanpa menggunakan rumput Fimbristylis sp. dan R1: menggunakan rumput Fimbristylis sp., diperoleh 10 kombinasi perlakuan, masing-masing diulang 3 kali. Hasil penelitian menunjukkan pemanfaatan Fimbristylis sp. meningkatkan jumlah populasi bakteri dan penurunan nilai Total Petroleum Hidrokarbon (TPH). Namun semakin banyak limbah minyak bumi yang ditambahkan maka pertumbuhan Fimbristylis sp. semakin rendah 
Stimulate The Growth of Rice Using Endophytic Bacteria from Lowland Rice Plant Tissue Nuni Gofar; Hary Widjajanti; Neni Marlina
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 12, No 2 (2015)
Publisher : Faculty of Agriculture, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v12i2.250

Abstract

Exploration and selection of endophytic bacteria from healthy food crops grown in lowland ecosystem is important to be conducted in order to get growth-stimulating endophytic bacteria at soil with low fertility level so that capable to optimize initial growth of food crops and subsequently can increase productivity level of lowland soil.The research objective was to isolate and to test the IAA-producing endophytic bacteria isolate in stimulating the rice crop growth at lowland area. Endophytic bacteria are isolated from tissues of rice, corn and peanut crops which grown at shallow swamp land in Ogan Ilir and Ogan Komering Ilir Districts, South Sumatra, Indonesia. There was nine isolates of nitrogen-fixer endophytic bacteria that capable to contribute IAA phytohormone into their growth media. The P31 isolate from rice crop tisssue of 2 months old produce the best rice sprouts than other isolates. This isolate can contribute of about 10 mg kg-1 IAA to its growth medium  and increase the crowns dry weight and the roots dry weight respectively with magnitudes of 133% and 225% compared to control treatment. Concentration and absorbtion of N for rice crops innoculated with P31 isolates had increased by 169% and 400%, recpectively. The P31 isolates had been identified as Burkholderia pseudomallei (also known as Pseudomonas pseudomallei).
EKSPLORASI BAKTERI ANTAGONIS ASAL JARINGAN DAN RIZOSFER TANAMAN KARET UNTUK MENEKAN PERTUMBUHAN BAKTERI PROTEOLITIK PADA BAHAN OLAHAN KARET (BOKAR) Nuni Gofar; Munawar Munawar; Hary Widjajanti; Angga Prasetya Mulya
Jurnal Ilmu Tanah dan Lingkungan Vol 16 No 2 (2014): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.176 KB) | DOI: 10.29244/jitl.16.2.61-66

Abstract

Lateks merupakan komoditas pertanian andalan dan merupakan sumber penerimaan devisa negara Indonesia yang cukup penting, namun pada saat penyimpanannya menyumbangkan bau tidak sedap ke udara. Penelitian ini bertujuan untuk mengeksplorasi bakteri antagonis asal jaringan dan rizosfer tanaman karet yang mampu menekan pertumbuhan mikroba proteolitik penyebab bau tidak sedap pada bahan olahan karet (bokar). Bakteri proteolitik penyebab bau tidak sedap diisolasi dari berbagai masa simpan bokar. Bakteri antagonis diisolasi dari jaringan dan rizosfer tanaman karet. Kemampuan bakteri antagonis menekan pertumbuhan bakteri proteolitik dilakukan dengan uji daya hambat dalam medium agar (NA). Diameter zona bening yang terbentuk merupakan indikasi kemampuan bakteri antagonis dalam menghambat aktivitas bakteri proteolitik. Dari hasil penelitian ini diperoleh 2 isolat bakteri yang berpotensi dikembangkan sebagai bakteri antagonis penghambat aktivitas bakteri proteolitik perombak bokar dalam proses penyimpanan. Kedua bakteri antagonis tersebut adalah isolat D1U1 yang berasal dari daun tanaman karet dan isolat A2U2 yang berasal dari akar tanaman karet, yang menghasilkan rata-rata zona hambat terluas berturut-turut berdiameter 11.37 dan 11.29 mm.
Co-Authors A. Wulandari A. Wulandari Abdul Madjid Agung Bimantara Agustina Alfarado, Daniel Alfia Rahma Kurniawati Ali Djamhuri Andika Puspita Dewi Angga Prasetya Mulya Aria Yanka Paula Putri Arum Setiawan Arum Setiawan Asiandu, Angga Puja Bambang Yudono Budiono Budiono Dade Jubaedah Dedi Rohendi Dedik Budianta E. Patriono E. Patriono Eka Amelia Elfita Elfita Elfita Elfita Elfita Elfita Elisa Nurnawati Ervina Mukharomah, Ervina Fitrya Fitrya Genti, Karta Sari Gusti Ayu Widayanti Handayani, Harminuke Eka Harminuke Eka Handayani Hary Wahyudi Heni Yohandini Laila Hanum Maraulina, Hotly Mardiyanto Mardiyanto Mardiyanto Maria Lusia Marini Wijayanti Maulida Harwati Meiyerani, Jeni Melki Melki Muharni Muharni Munawar Munawar Munawar Munawar Muslim Muslim Nabila Saraswati Neni Marlina Ni Luh Putu SR Nina Tanzerina Nita Aminasih Noviyanto NUNI GOFAR Nur Arifah Nurkayah, Nurkayah Oktiansyah, Rian Permana, Muhammad Aditya Putri, Amanda Rahmaniah Putri, Aria Yanka Paula Putri, Hanindita Alifia Rahmawati Ridwan Riris Aryawati Rosmania, Rosmania Rozirwan . Salni Salni Salni Saputra, Ade Bayu Sarno . Sarno Sarno Singgih Tri Wardana Siti Alharzsa Siti Yuliani Sri Pertiwi E Sutandar, Vivi Hendra Syarifah Tanbiyaskur Ummi Hiras Habisukan, Ummi Hiras Wuriesyliane Wuriesyliane Yustina Hapida, Yustina