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All Journal HAYATI Journal of Biosciences Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology UNEJ e-Proceeding Indonesian Journal of Chemistry Jurnal Aplikasi Teknologi Pangan Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
ADI YULANDI
Faculty Of Biotechnology Atma Jaya Catholic University Of Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Veterinary

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Journal : HAYATI Journal of Biosciences

 1 
Genetic Diversity of Klebsiella spp. Isolated from Tempe based on Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR) TATI BARUS; IVAN HANJAYA; JOANITA SADELI; BIBIANA W LAY; ANTONIUS SUWANTO; ADI YULANDI
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (625.021 KB) | DOI: 10.4308/hjb.20.4.171-176

Abstract

Tempe is an Indonesian fermented food prepared by fermenting dehulled cooked soybeans with Rhizopus oligosporus. Many types of bacteria are also involved during tempe fermentation, and one of these is Klebsiella spp.  Some isolates of K.  pneumoniae produces vitamin B12 in tempe but it has also been classified as an opportunistic pathogen. For this reason Klebsiella spp. in tempe is important to be studied. The aim of this study was to investigate the genetic diversity of Klebsiella spp. from tempe employing ERIC-PCR method. Sixty-one isolates of Klebsiella have been isolated from sixteen tempe producers  in Bogor, Jakarta, Malang, Tengerang, Bandung and Cianjur. 63F and 1387R primers were used to amplify 16S rDNA sequences, and 1R and 1F primers were used for ERIC analysis. The results of this research showed that sixty-one strains of Klebsiella were clustered into 17 groups. Based on ERIC-PCR analysis, isolates of Klebsiella could be grouped into different profiles which some of these groups consisted of isolates with identical ERIC-PCR profiles. Several identical ERIC-PCR profiles were found in tempe from the same producer. There was no correlation observed between genetic similarity  among isolates with the origin of tempe.
Diversity of Amylase-Producing Bacillus spp. from “Tape” (Fermented Cassava) BARUS, TATI; KRISTANI, AMANDA; YULANDI, ADI
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.662 KB) | DOI: 10.4308/hjb.20.2.94

Abstract

Fermented cassava or “Tape” is one of traditional Indonesian fermented food. The quality of “Tape” is determined by microorganisms involved during fermentation process. It was reported that Bacillus subtilis determined the quality of cassava “Tape”. The most common way to identify species is by using 16S rRNA gene.  This gene contains conserved regions as unique sequence which is relative among species. It has been widely used as a reliable molecular marker for phylogeny identification. Therefore, the aim of this research was to study diversity of amylase-producing Bacillus spp. from “Tape” based on 16S rRNA gene sequences. Bacillus spp. were isolated from “Tape” from several area in Indonesia i.e. Jakarta, Bandung, Cianjur, Subang, Rangkas Bitung, and Kediri. Amplification of 16S rRNA gene used 63f and 1387r primers. This research showed that based on 16S rRNA gene sequences, twenty-six of amylase-producing Bacillus spp. isolates were divided into four groups. All isolates were identified as species either B. megaterium, B. subtilis, B. amyloliquefaciens, or B. thuringiensis.
Morphological and Molecular Identification of Culturable Arbuscular Mycorrhizal Fungi (AMF) Associated with Pternandra azurea from Martabe Batang Toru Forest, North Sumatra, Indonesia Maulani, Nail Izzatul; Sukarno, Nampiah; Yulandi, Adi; Listiyowati, Sri; Kramadibrata, Kartini; Subagya, Mahmud; Anwar, Syaiful
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1240-1250

Abstract

Arbuscular Mycorrhizal Fungi (AMF) form a mutualistic symbiosis with almost all host plant species and exist in various ecosystems. Studies of AMF diversity in Sumatra tropical rainforest plants are still limited. This research aimed to isolate and identify AMF associated with Pternandra azurea from Martabe Batang Toru forest, North Sumatra. The spores were obtained from the rhizosphere of P. azurea and propagated by trap culture using Pueraria javanica, Sorghum vulgare, and Zea mays. AMF spores were isolated using wet sieving and decanting techniques, then inoculated into roots of P. javanica grown in sterile zeolite to obtain a single-species culture. The AMF were identified based on spore morphology and molecular analysis using AML1/AML2 specific primers. A total of 13 AMF single-species cultures were obtained, and based on morphological characteristics, they were identified as Claroideoglomus lamellosum, C. claroideum, Acaulospora rehmii, A. longula, and Glomus ambisporum. Further identification using molecular analysis, the cultures were identified as C. etunicatum, A. spinosa, A. longula, and G. ambisporum. Molecular identification resulted in different AMF species from morphological identification. Claroideoglomus was dominant AMF observed. All cultures formed internal hyphae, arbuscules, and vesicles within the roots. The AMF cultures obtained can be used as biofertilizers to restore degraded ecosystems.
Co-Authors A A Hermosaningtyas A A Hermosaningtyas, A A AMANDA KRISTANI Anastasia Tatik Hartanti Antonius Suwanto Anugrah, Daru Seto Bagus BIBIANA W LAY David Tandjung DIANA ELIZABETH WATURANGI Diana Lestari, Diana IVAN HANJAYA Jason Wiranata Sanjaya JOANITA SADELI Kartini Kramadibrata Laura Virdy Darmalim Liana Dewi Nuratikah Maulani, Nail Izzatul Nampiah Sukarno Nurita Toruan-Mathius Nurwarrohman Andre Sasongko Parsaoran Siahaan Permono Adi Putro Sabrina, Feliciana Sheila Sutanto Sheila Sutanto, Sheila Sri Listiyowati Stella Magdalena Subagya, Mahmud Suryadi, Jimmy Syaiful Anwar Tati Barus Vanessa, Valencia Vivitri Dewi Prasasty Wahyunanto, Irang Waturangi, Diana E Wijaya, Laura Wijaya, Natalie Chandra Yogiara Yogiara