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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Farmasi Klinik Indonesia Indonesian Journal of Tropical and Infectious Disease The Indonesian Biomedical Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Respirologi Indonesia YARSI Medical Journal PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal Ilmiah Kesehatan Promotif
Irawan Yusuf
Faculty of Medicine, Hasanuddin University, Makassar-Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Medicine & Pharmacology Nursing Public Health

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The Renal Toxicity of Welding Fumes in Heavy Equipment Manufacturer Workers Mulyana, Mulyana; Adi, Nuri P.; Kurniawidjaja, Meily Kurniawidjaja; Pratami, Vani N.; Wijaya, Andi; Yusuf, Irawan
Indonesian Journal of Clinical Pharmacy Vol 4, No 3 (2015)
Publisher : Indonesian Journal of Clinical Pharmacy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.437 KB) | DOI: 10.15416/ijcp.2015.4.3.199

Abstract

Exposure to welding fumes in the workplace has been associated with decreasing renal function. We studied renal function parameters in men workers from heavy equipment manufacturer exposed to welding fumes. This study aimed to evaluate renal function status among worker exposed to welding fumes. A case-control design, random study, was conducted among welder (35 subjects) and nonwelder (35 subjects) with more than 1 years experience in the same job task in a heavy equipment manufacturer. All subjects were completed physical examination, informed consent, questionnaire and laboratory tests. Renal function was easured as creatinine serum using enzimatic method. Urinary heavy metals level was analyzed using inductively coupled plasma mass spectrometry. Comparisson analysis between group was performed to determined median level for each variable. Linear regressionmodel was developed to predict renal function function parameter status urinary heavy metals level as variable. This study showed there were higher creatinine serum, chromium, iron, manganese and nickel in welder than non welder(p<0.05). After multivariate analysis, urinary nickel is a predictor for renal function status among welder. Exposure to welding fumes was significantly correlated with renal function status in welder. Nickel is the predictive variable for renal function. Although statistically significant but in clinical field needs carefully interpreting data.Keyword: Nickel, renal function, welder, welding fumesToksisitas Renal Uap Las pada Pekerja Industri Alat BeratPajanan uap las di tempat kerja telah dihubungkan dengan penurunan fungsi ginjal. Dalam penelitian ini, kami menguji status fungsi ginjal pada pekerja pria yang terpajan uap las di industri alat berat. Penelitian ini mengevaluasi status fungsi ginjal pada pekerja terpajan uap las, dengan desain kontrol kasus, acak, dilakukan pada 35 subjek pengelas dan 35 bukan pengelas yang telah bekerja paling tidak 1 tahun dengan jenis pekerjaan yang sama di industri alat berat. Seluruh subjek telah melakukan pemeriksaan fisik,pengisian form kesediaan setelah penjelasan, kuesioner, dan pemeriksaan laboratorium. Pemeriksaan kadar kreatinin dilakukan dengan metode enzimatik. Pemeriksaan kadar logam berat dalam urin dengan inductively copled plasma mass spectrometry. Dilakukan analisis perbandingan untuk membedakan nilai median antar kelompok. Dilakukan juga analisis multivariat untuk menentukan variabel prediksi. Hasil penelitian memperlihatkan bahwa status fungsi ginjal pada pekerja las lebih rendah dibandingkan dengan bukan pengelas. Nikel urin merupakan variabel prediksi yang bermakna terhadap penurunan fungsi ginjal. Walaupun secara statistik diketahui bermakna, namun dalam penggunaan klinis harus dilakukan interpretasi dengan hati-hati.Kata kunci: Fungsi ginjal, nikel, pengelas, uap las
STATUS ANTIOKSIDAN GLUTATION PADA PASIEN TUBERKULOSIS PARU DI BALAI KESEHATAN PARU (BKPM) MAKASSAR Yuniastuti, Ari; Yusuf, Irawan; Massi, Muh Nasrum; -, Budu
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2745

Abstract

AbstrakAntioksidan merupakan senyawa untuk pertahanan tubuh host terhadap radikal bebas. Pada pasien tuberkulosis paru (TB paru), rendahnya antioksidan menyebabkan kerusakan jaringan. Salah satu antioksidan tubuh adalah glutation. Rendahnya glutation berhubungan dengan gangguan sistem imun, sehingga menyebabkan keparahan penyakit pada pasien TB paru. Penelitian ini bertujuan mengetahui kadar glutation plasma penderita tuberkulosis paru. Desain penelitian ini adalah kohort prospektif. Sampel TB paru dipilih di Balai Besar Kesehatan Paru Masyarakat (BBKPM), Makassar yang sesuai dengan kriteria inklusi dan eksklusi dan sesuai kadatangan (consecutive sampling). Dua mililiter darah diambil dari setiap sampel, kemudian dilakukan pemeriksaan kadar glutation dengan ELISA. Hasil penelitian menunjukkan bahwa kadar glutation sampel pasien TB paru sebelum pemberian obat antituberkulosis (OAT) sebesar 0,483830,0344 mM dan setelah pemberian OAT sebesar 0,494650,0371 mM. Setelah pemberian OAT kadar glutation mengalami peningkatan sebesar 2,2% meskipun masih lebih rendah dari kadar glutation normal.AbstractAntioxidants prevent hosts against free radicals. In pulmonary tuberculosis (TB) patients, the low level of antioxidants may cause tissue damage in oxidative hosts. One of antioxidants is glutathione. The poor glutathione level correlates with immune system disorder. The current study aimed to determine glutathione plasma levels in pulmonary tuberculosis patients. The study design was prospective cohort. Pulmonary TB samples were obtained from patients in the Lung Health Centre (BBKPM) in Makassar, and they were selected based on the inclusive and exclusive criteria and on their admittance times (a consecutive sampling technique). Two mililiters of blood was taken from each sample and the glutathione level was examined using ELISA. The study indicated that the glutathione level of the pulmonary TB patients before administered with antitubeculosis drug was 0.483830.0344 mM and after antituberculosis drug was 0.494650.0371 mM. After administration of antituberculosis drug the glutathione level increased by 2.2%, although still lower than normal glutathione level.
Intra Arterial Heparin Flushing Increases Manual Muscle Test – Medical Research Councils (MMT-MRC) Score in Chronic Ischemic Stroke Patient Putranto, Terawan Agus; Yusuf, Irawan; Murtala, Bachtiar; Wijaya, Andi
BALI MEDICAL JOURNAL Vol 5 No 2 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.936 KB)

Abstract

Background: Muscle strength impairment in stroke patient affect the patient daily life, especially when it occurs on the extremities muscles. Manual Muscle Testing (MMT) is an examination method to measure muscle strength using standardized scoring. Objective: To find possible improvement of Manual Muscle Test (MMT) Score after administration of Intra Arterial Heparin Flushing in chronic ischemic stroke patient. Method: This is an experimental study using pretest-posttest group design, with randomized controlled clinical trial, conducted among patients in Cerebrovascular Center Unit in Army Central Hospital Gatot Soebroto starting from February 2014. With 75 patients included in this study. The examination of muscle strength was done by trained physicians. The MMT score were taken before and after the IAHF procedure is conducted. Results: This study found higher score of MMTMRC scoring system on chronic stroke patient with IAHF procedure (mean MMT-MRC Score = 6,05 point. With p
POPULASI BAKTERI PADA TANAH BEKAS BUANGAN LIMBAH MERKURI TAMBANG EMAS DI KABUPATEN BOLAANG MONGONDOW: PENELITIAN PENDAHULUAN Fatimawali, Fatimawali; Kepel, Billy; Yusuf, Irawan; Natsir, Rosdiana; Baharuddin, Fatmawaty
Jurnal Kedokteran YARSI Vol 17, No 2 (2009): MEI - AGUSTUS 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.068 KB) | DOI: 10.33476/jky.v17i2.207

Abstract

Mercury is one of the most toxic heavy metals found in nature. Athough adverse health effect of mercury have been known for a long time, exposure to mercury continues and is even increasing in some areas, for example, mercury is still used in gold mining in many parts of North Sulawesi Province. Most of the soil and aquatic bacteria that are continuously exposed to mercury usually develop a genetic adaptation to resist the toxicity of this compound. Bacteria have a specific operon called merOperon that functions to coordinate genes coding for proteins and enzymes involved in mercury disposal and detoxification. Therefore, this preliminary study aims to isolate and identify bacteria collected from gold mining area in the district of Bolaang Mongondow. Bacteria were isolated from soil samples collected from three locations of the gold mining waste disposal and the isolated bacteria were grown in agar media. Identification of the grown bacteria were then be performed using morphological, physiological and biochemical tests. The results showed that 36 bacteria were successfully isolated, of which, 11 isolates were gram positive bacteria and the remainders were gram negative. All isolates showed motility and all could be grouped into 4 species i.e. Bacillus sp., Escherichia coli, Enterobacter cloacea, and Enterobacter aerogenes.
LYMPHOCYTE RESPONSE TO Mycobacterium leprae ANTIGENS IN REVERSAL REACTION STATE OF LEPROSY Yusuf, Irawan; Agusni, Indropo
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 4 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.68 KB) | DOI: 10.20473/ijtid.v5i4.2007

Abstract

Reversal Reaction (RR) in Leprosy is a sudden inflammatory episode in the chronic course of the disease due to rapid change of cellular immunological status. The aim of the study is to measure the in vitro results of Lymphocyte Stimulation Index (LSI) RR leprosy derived lymphocytes after challenged with M.leprae antigens. Twenty three Borderline Leprosy with RR and 11 Borderline Leprosy patients without RR were included in the study. Peripheral Blood Mononuclear Cells (PBMC) were separated from peripheral blood of these patients using Ficol-Hypaque column and cultured in laboratory. Using the colorimetric tetrazole (MTT) method these lymphocytes were challenged with PHA, Dharmendra antigen (1/100 and 1/10 dilutions), LAM (50 and 100 nanograms). Stimulation Index were calculated and superanatans were collected for measuring the IFN-γ and IL-10 production (ELISA). All of lymphocytes from RR patients showed higher Stimulation Index after challenged with the five M.leprae antigens compared to lymphocytes from non RR patients (p <0.05) . IFN-γ and IL-10 also increased but not significant (p>0.05). It is concluded that lymphocytes of leprosy patients during RR state are more sensitive to antigenic stimuli compared to non-RR leprosy patients. Further extended studies are needed to determine the "cut off” value of lymphocyte Stimulation Index that is useful for clinicians in the field in the prediction of RR before starting anti leprotic treatment.
CYP2E1 POLYMORPHISMS AND SUSCEPTIBILITY TO ANTI-TUBERCULOSIS DRUG-INDUCED HEPATOTOXICITY IN INDONESIA POPULATION Suhuyanly, Nella; Bakri, Syakib; Yusuf, Irawan; Massi, Muh Nasrum
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 1 (2025): APRIL 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i1.41251

Abstract

Pengobatan Tuberkulosis (TB), berdasarkan penggunaan isoniazid (INH), rifampisin (RMP) dan pirazinamid (PZA), terbukti menyebabkan hepatotoksisitas yang diinduksi oleh obat (Drug Induced Hepatotoxicity/DIH). Penelitian terbaru menunjukkan bahwa variasi genetik dapat dikaitkan dengan risiko DIH, seperti status asetilator INH, yang terkait dengan polimorfisme N-asetil transferase (NAT) 2, yang mana asetilator lambat pada umumnya lebih rentan terhadap efek samping obat. Proporsi asetilator cepat dan lambat sangat bervariasi pada populasi dengan etnis atau geografis yang berbeda yang telah dijelaskan dalam berbagai penelitian, tetapi, masih ada informasi yang terbatas dalam populasi kita. Tujuan dari penelitian ini adalah untuk menyelidiki kontribusi polimorfisme CYP2E1 terhadap DIH anti-TB pada populasi kami. Penelitian kasus kontrol ini dilakukan di Rumah Sakit Cipto Mangunkusumo, Jakarta dan Rumah Sakit Omni Alam Sutera, Tangerang, Indonesia dari Januari 2015 - Desember 2016. Kami merekrut 35 orang dengan DIH dan 34 orang tanpa DIH. Profil fungsi hati lengkap, bilirubin total serum, bilirubin tidak langsung, dan bilirubin langsung diukur. Kami melakukan genotipe polimorfisme CYP2E1 rs3813867, rs2031920 dan rs6413432. Kami menemukan bahwa polimorfisme CYP2E1 c1/c1 (tipe liar homozigot) pada 61 subjek (88,4%) dan tidak ada perbedaan yang signifikan secara statistik antara tipe liar homozigot dan varian yang jarang (mutan alel) dalam kejadian DIH (95% CI 0,403 - 8,383, P = 0,338). Kami mengusulkan bahwa polimorfisme CYP2E1 tidak dapat membantu dalam memprediksi kerentanan terhadap hepatotoksisitas yang diinduksi oleh obat anti-tuberkulosis pada populasi di Indonesia.
PENGARUH COLD PRESSOR TEST TERHADAP TEKANAN DARAH DAN KADAR KORTISOL DARAH Rahayu, Irma; Yusuf, Irawan; Idris, Irfan
Jurnal Ilmiah Kesehatan Promotif Vol. 9 No. 2 (2025): Juni 2025
Publisher : Institut Toraja Raya Indonesia (ITRI)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cold pressor test (CPT) adalah satu tes yang dapat digunakan untuk mengukur reaktivitas tekanan darah terhadap stimulus dingin eksternal yang telah umum digunakan untuk mengevaluasi reaktivitas kardiovaskular terhadap stres pada subjek normotensi dan hipertensi dimana reaktivitas kardiovaskular dipercaya dapat memprediksi resiko terjadinya hipertensi dikemudian hari dengan melibatkan kortisol. Penelitian ini bertujuan untuk mengetahui hubungan antara peningkatan tekanan darah dengan kadar kortisol terhadap pemberian CPT. Jenis penelitian merupakan penelitian eksperimental dengan desain One-Group Pretest - Posttest Design, dengan jumlah subjek sebanyak 42 orang yang berusia sekitar 18-73 tahun dengan cara pengambilan sampel teknik purposive sampling. Penelitian dilakuan di laboratorium Hasanuddin University Medical Research Centre dengan cara memberikan stressor dingin pada lengan dengan merendam kedalam air es dengan suhu 3-5 0C selama 1 menit dan di ambil darah serta mengukur tekanan darah sebelum dan setelah perendaman tangan kiri hingga melewati batas pergelangan. Selanjutnya darah yang diambil diukur kadar kortisolnya dan dibandingkan dengan hasil pengukuran tekanan darah. Hasil penelitian menunjukkan terdapat perubahan yang signifikan pada tekanan darah terhadap cold pressor test. Sedangkan pada perubahan tekanan darah dan kadar kortisol terdapat perubahan meskipun tidak signifikan.
High-yield and Functional IgY Anti-S1 SARS-CoV-2 Delta Variant from Chicken Immunized with In-house Recombinant Protein Zarkasie, Kamaluddin; Ginting, Teridah Ernala; Poeloengan, Andrea Hynan; Andriani, Febi; Lages, Aksar Chair; Cornelia, Vina; Nugraha, Anggah; Rosidi, Bustanur; Indrasari, Setyarina; Nidom, Chairul Anwar; Yusuf, Irawan
The Indonesian Biomedical Journal Vol 17, No 4 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i4.3693

Abstract

BACKGROUND: During COVID-19 pandemic, effective preventive and therapeutic strategies was urgently necessary, especially with the emerging Delta variants. Previous existing interventions have some limitations, and passive immunization using immunoglobulin Y (IgY) is considered viable. However, the potential of IgY antibodies remains underexplored locally. Therefore, a feasible study was conducted to produce IgY against the SARS-CoV-2 Delta variant from chickens raised on a farm, instead of a laboratory environment, by using in-house recombinant protein, to offer a promising low-cost alternative for passive immunization.METHODS: In-house HEK293 cell line was produced by secreting recombinant S1 subunit protein of SARS-CoV-2 Delta variant. The protein was confirmed by western blot and used as an immunogen in two chickens. IgY was extracted from egg yolks. Titers and neutralization activity of IgY against SARS-CoV-2 Delta variant were confirmed by enzyme-linked immunosorbent assay (ELISA) and surrogate virus neutralization test (sVNT). The functionality of IgY was then tested on lysates from COVID-19 patients' nasopharyngeal swabs with dot blot method.RESULTS: The high anti-S1 IgY titers and neutralization activity was confirmed following immunization with 1 mg immunogen at 2–3-week intervals. IgY titers varied between chickens and time points. The mean titers showed significant increase after the fifth immunization (1.21, 1.72 and 1.48; with p<0.05 for all). Neutralization activity appeared after the second immunization and was significant after the third immunization (31.9%, 34.95%, 26.9%, 47.6%, 54.95% and 57.3%; with p<0.05 for all). The results of this study showed that the extracted IgY reacted to COVID-19 patients' nasopharyngeal swabs lysates.CONCLUSION: A high-yield and functional IgY anti-S1 SARS-CoV-2 Delta variant can be produced from chicken immunized with an in-house recombinant protein. This can be an alternative for affordable and effective IgY production during public health emergencies.KEYWORDS: IgY, SARS-CoV-2, S1 subunit protein, egg yolk, chicken
Effects of Active Hexose Correlated Compound (AHCC®) on Clinical and Laboratory Response of Pulmonary Tuberculosis Patients Madolangan, Jamaluddin; Yusuf, Irawan; Wahyudin, Elly; Djaharuddin, Irawaty
Jurnal Respirologi Indonesia Vol 45 No 4 (2025)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v45i4.820

Abstract

Backgrounds: Active hexose correlated compound (AHCC), an α-glucan extracted from Lentinula edodes, is known for its immunomodulatory properties. It is suspected that the strong nutrients contained in AHCC are very safe and without side effects, including in tuberculosis patients. This study evaluated the effect of AHCC on clinical and hematologic responses in pulmonary tuberculosis (TB) patients Methods: A double-blind randomized controlled trial was conducted involving 60 pulmonary TB patients receiving standard anti-TB therapy. Participants were randomly assigned to receive either AHCC (3 g/day) or a placebo for six months. Clinical and laboratory parameters were assessed before and after treatment. Results: Patients in the AHCC group showed greater improvement in clinical symptoms (reduced cough, improved appetite, and weight gain) and significant enhancement of hematological and immunological markers compared with placebo (P<0.05). AHCC supplementation significantly increased CD4⁺ and CD8⁺ lymphocyte counts, suggesting improved immune recovery and modulation of host defense against Mycobacterium tuberculosis infection. Conclusion: AHCC supplementation alongside standard anti-TB therapy improves clinical outcomes and immune responses in patients with pulmonary tuberculosis.
Co-Authors Adi, Nuri P. Aksar Chair Lages, Aksar Chair Andi Fachruddin ANDI WIJAYA Andriani, Febi Anwar Santoso Ari Yuniastuti Bachtiar Murtala Baharuddin, Fatmawaty Bertha Pangaribuan Billy Kepel Budhianto Suhadi Budu - Cornelia, Vina Dewi Muliaty Djaharuddin, Irawaty Elly Wahyudin Fanny Iskandar, Fanny Fatimawali . Fatmawaty Badaruddin Franky Hartono Ginting, Teridah Ernala Indrasari, Setyarina INDROPO AGUSNI IRFAN IDRIS Irma Rahayu, Irma Kamaluddin Zarkasie L. Meily Kurniawidjaja Madolangan, Jamaluddin Mansur Arif, Mansur Marita Kaniawati Muchtaruddin Mansyur Muh. Nasrum Massi Mulyana Mulyana Natsir, Rosdiana Nidom, Chairul Anwar Nugraha, Anggah Poeloengan, Andrea Hynan Pratami, Vani N. Rianto Setiabudy Rosidi, Bustanur Septelia I. Wanandi Suhuyanly, Nella Suryani As’ad Syakib Bakri Terawan Agus Putranto, Terawan Agus