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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Mutiara Medika: Jurnal Kedokteran dan Kesehatan Journal of Community Empowerment for Health
. Suhartini
Departemen Ilmu Kedokteran Forensik Dan Medikolegal, Fakultas Kedokteran, Kesehatan Masyarakat, Dan Keperawatan Universitas Gadjah Mada, Yogyakarta, Indonesia
Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Analysis of Enzyme Activity of Alcohol Dehydrogenase and Alcohol Dehydrogenase 3 (ADH3) Gene Polymorphism of Alcoholics and Non-Alcoholics in Indonesia. . Suhartini; . Mustofa; Yudha Nurhantari; Bambang Udji Djoko Rianto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 2 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (766.347 KB) | DOI: 10.19106/JMedSci004802201604

Abstract

ABSTRACTAlcohol is an addictive substance that is often misused worldwide, including in Indonesia. Ninety percent of the alcohol that enters the body will be metabolized in the liver using the alcohol dehydrogenase (ADH) enzyme. It is important to determine the activity of ADH enzyme and ADH3 gene polymorphism on alcoholics and non-alcoholics in Yogyakarta, Indonesia. The aim of the study is to determine ADH activity and identify ADH3 gene polymorphism of alcoholics and non-alcoholics in Yogyakarta, Indonesia. This study was an observational study with a cross-sectional design method. Blood samples were taken from 71 Javanese alcoholics and 71 non-alcoholics of Javanese descent in Yogyakarta, Indonesia. The participants were initially requested to sign an informed consent form. Examination of ADH enzyme activity used the spectrophotometry method and ADH3 gene polymorphism was assessed with PCR-RFLP using Ssp I restriction enzyme. The activity of ADH enzyme in all individuals appeared to be a slower type. The average of the ethanol value of alcoholics and non-alcoholics were 0.05554 mM and 0.0758 mM respectively. Gene type of alcoholics were ADH3*2(75.4%), ADH3*1/3*2(21.5%), and ADH3*1(3.1%), and non-alcoholics were ADH3*2(88.6%), ADH3*1/3*2(10.0%), and ADH3*1(1.4%). There were no significant differences between the activity of ADH with polymorphism of ADH3 gene in either alcoholics and non-alcoholics (p>0,05). Conclusion: The activity of ADH enzyme in all participants appeared to be a slower type. Most of the ADH3 gene polymorphism of alcoholics and non-alcoholics were both ADH3*2 (75.4% and 88.6%). There was no differences of ADH enzyme activity with ADH3 gene polymorphism between alcoholics and non-alcoholics of Javanese population in Yogyakarta, Indonesia.
The analysis of cell damage of liver and kidney among alcoholics in Yogyakarta, Indonesia S Suhartini; Hendro Widagdo; Yudha Nurhantari; Idha Arfianti Wiraagni
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 3 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.818 KB) | DOI: 10.19106/JMedSci005103201908

Abstract

Alcohol tends to disrupt the organs function of human body, even can cause serious and chronic damage. In Indonesia, the data on cell damage taken from organs including the livers and kidneys among alcoholics are still practically unknown. The aim of this study was to compare the differences of organs’ cell disturbance between alcoholics and non-alcoholics in Yogyakarta, Indonesia. A crosssectional study was conducted among 197 people in Yogyakarta, including 96 alcoholics and 101 non-alcoholics. The material of the study was taken from venous blood samples. A kinetic photometric test was conducted to obtain data on blood-chemical markers’ value of livers (SGOT, SGPT, and GGT), and kidneys (BUN and serum creatinine). The data werethen analyzed by Chi square test.From a total of 96 alcoholics, 83.6% are males and 16.4% are females who consumed alcohol for an average of 16 years, with 51.6% routinely consuming it daily. The kind of alcohol they consumed was single-brand (59.8%). Based on 25th percentile (GGT), on 50th percentile (SGOT, GGT), and on 75th percentile (SGPT, GGT), the alcoholics had higher proportion value of liver marker disturbance than non-alcoholics which was statistically significant (p<0.05). Based on the 50th and 75th percentiles, the alcoholics also had higher proportion value on kidney marker (BUN) damage than non-alcoholics which was statistically significant (p<0.05). There were significant differences in GFR values between males and females (p<0.05), males had kidney cell damage 7.9 times more than females. There was no significant difference in the value of GFR between alcoholics and non-alcoholics. In conclusion, the alcoholics hassignificantly higher proportion value of blood-chemical markers than that non-alcoholics. The GFR values between males and females are also significantly different, and males had kidney cell damage 7.9 times more than that of females.
The Correlation of Cyp2e1 Genetic Polymorphism on Alcohol Drinking Habits in Papuan Ethnicity Mardhatillah Marsa; Yudha Nurhantari; Suhartini Suhartini; Tri Ratnaningsih
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 2 (2021): July
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i2.11579

Abstract

Alcohol abuse is associated with genetic factors and is influenced by certain races and ethnicities. CYP2E1, which works on the endoplasmic reticulum, produces an enzyme that plays a significant role in alcohol metabolism. In relation to it, this study aims to identify the polymorphisms of CYP2E1*5B and CYP2E1*6 genes in alcohol drinkers of Papuan Ethnics. A total of 39 Papuans were analyzed for alcohol drinking habits. Alcohol drinkers were found to be 29 people (74.4%), and 10 people (25.6%) were non-drinkers. The drinkers mainly were late teenagers (89,7%) and males (69,2%). The CYP2E1*5B genotypes were c1/c1 as 94.9% and c1/c2 as 5.1%. Meanwhile, the CYP2E1*6 T/T genotypes were 56.4%, and T/A genotypes were 43.6%. The odd ratio for CYP2E1*5B were 18,5 and 7,7 for CYP2E1*6. p0,05 for CYP2E1*5B and CYP2E1*6 gene polymorphisms for alcohol drinking behavior in the form of frequency, duration, type, and volume of alcohol consumed. Furthermore, c1/c1 and c1/c2 genotype polymorphisms were in CYP2E1*5B; T/T and T/A genotypes were in CYP2E1*6 of Papuan ethnic at Yogyakarta. In conclusion, genotype c1/c1 had 18,5 times of the possibility of being alcoholic drinkers, and genotype T/T had 7,7 times of the possibility of being alcoholic drinkers in Papuan ethnic. It indicated that the type of genotype statistically did not significantly affect alcohol drinking behavior on the subject.
Karakteristik performance individu muda di Desa Ngablak dan hubungannya dengan penyalahgunaan NAPZA Suhartini Suhartini; Neni Trilusiana Rahmawati; Yudha Nurhantari; Hendro Widagdo; Idha Arfianti Wira Agni; Martiana Suciningtyas; Wikan Basworo
Journal of Community Empowerment for Health Vol 2, No 1 (2019)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.116 KB) | DOI: 10.22146/jcoemph.38574

Abstract

Alcohol, narcotics, psychotropic substances and other addictive substances are substances that are often misused. The majority of drug users are adolescents with age ranging from 13 - 17 years old at which abuse can affect body composition. Considering the role of the younger generation as the nation’s successors, it sparks the urgency to alleviate this negative behavior. One of the measures that can be done is by shifting the negative activities to positive ones such as sports. In order to do this, it is necessary to identify the performance characteristics of the youths to maximize their potential. This study aimed to find out the performance characteristics of the younger generation in Ngablak Village and their association with drug abuse. A total of 50 youth members in Ngablak Village, Ngablak Subdistrict, Magelang Regency were examined for anthropometry, physical (pulse, respiration rate, blood pressure, temperature), and somatotype associated with performance. Identification of subjects involved in alcohol and drug abuse was carried out using a questionnaire. The results were displayed descriptively. Statistical analyses (t-test or Mann Whitney U test) were used to compare performance characteristics between groups. Age of subjects ranged from 9 - 19 years and 50% were male. A total of 13 boys and 2 girls consumed alcohol. The results of physical examination showed the normal range in all subjetcs. The average age, height, and weight of male subjects who comsumed alcohol were higher than those who did not (p < 0,05). There was no difference in somatotype between adolescents who consumed alcohol and those who did not. The body type of male subjects based on measurements was the ideal type, whereas the female subjects had endomesomorphic body type. The tendency of consuming alcohol does not cause differences in body type in adolescents, both in male and female.
Hubungan timbal dan krom pada pemakaian pewarna batik dengan kadar hemoglobin dan packed cell volume pada pengrajin batik di Kecamatan Lendah Kulon Progo Pramudji Hastuti; Sunarti Sunarti; Prasetyastuti Prasetyastuti; Ngadikun Ngadikun; Tasmini Tasmini; Dianandha Septiana Rubi; Sri Sutarni; Indrasari Kusuma Harahap; Kusumo Dananjoyo; Suhartini Suhartini; Ida Bagus Gd. Surya Putra Pidada; Hendro Widagdo; Martiana Suciningtyas
Journal of Community Empowerment for Health Vol 1, No 1 (2018)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.051 KB) | DOI: 10.22146/jcoemph.39156

Abstract

ABSTRAK Cara membatik di Indonesia mulai banyak beralih dari pemakaian bahan alami menjadi bahan cat sintesis. Cat sintetis yang digunakan sering kali mengandung logam berat di antaranya timbal (Pb) dan kromium (Cr). Pemakaian logam berat dalam waktu lama akan mengganggu kesehatan. Penelitian ini dilakukan untuk melihat hubungan kadar logam Pb dan Cr dalam darah dan limbah dengan kadar hemoglobin (Hb) dan packed cell volume (PCV) serta mengetahui prevalensi gejala toksisitas yang dirasakan pada pekerja di tiga sentra batik di Kecamatan Lendah Kulon Progo. Sebanyak 76 pegawai di tiga sentra batik terdiri atas 37 orang dari sentra SB, 20 orang dari FA dan 19 orang dari SA ditentukan kadar Hb, PCV, kadar Pb dan Cr-nya dengan metode inductively coupled plasma (ICP). Air limbah lingkungan dari ketiga lokasi sentra batik diambil dan diperiksa kadar Pb dan Cr-nya. Hasil antara ketiga lokasi diujinormalitasnya dan dianalisis dengan analisis varian atau Kruskal-Wallis. Hubungan antara kadar Pb dan Cr baik darah maupun limbah dengan kadar logam berat dalam darah dianalisis dengan uji korelasi Pearson atau Spearman. Hasil dinyatakan berbeda bermakna jika p < 0,05. Gejala penyakit yang dialami pekerja yang diperkirakan berhubungan dengan toksisitas logam berat dihitung frekuensinya. Hasil pemeriksaan antropometri, tekanan darah, kadar Hb, PCV dan logam berat dari ketiga lokasi yang diperiksa tidak berbeda bermakna (p > 0,05). Kadar Pb darah dan krom di sentra SB dan FA berbeda bermakna dengan di sentra SA (p < 0,05). Tidak terdapat korelasi yang signifikan secara statistik antara kadar Pb darah, Cr darah, dan Pb limbah dengan kadar Hb dan PCV. Gejala iritasi kulit, pusing, dan nyeri sendi dirasakan oleh 36,8-48,7% responden. Kadar timbal dan krom darah pembatik di sentra SB dan FA lebih tinggi dari pada di sentra SA. Tidak terdapat hubungan bermakna antara toksisitas Pb dan Cr dengan kadar Hb dan PCV. Gejala toksisitas di antara pekerja batik diperkirakan dapat terjadi karena toksisitas logam berat kronis akibat pemakaian cat batik. KATA KUNCI batik; hemoglobin; kromium; sel darah; timbal ABSTRACT The dye used to color batik has changed from natural to synthetic materials in several places in Indonesia. Synthetic paints often contain heavy metals including lead (Pb) and chromium (Cr). Chronic use of heavy metals can affect health. This study was conducted to examine the relationship between blood and waste water levels of Pb and Cr with hemoglobin (Hb) and packed cell volume (PCV), and to determine how many workers felt the toxicity symptoms in three batik centers in Lendah, Kulon Progo. A total of 76 batik center employees, consisted of 37, 20, and 19 people from the SB, FA, and SA centers were recruited. Determination of Hb, PCV, Pb and Cr levels were conducted with the inductively coupled plasma (ICP) method. Waste water from the three batik centers is taken and examined. The data were analyzed by Oneway ANOVA or Kruskal-Wallis. Correlation analyses were performed with Pearson or Spearman correlation test. Results were considered significant if p < 0.05. Disease symptoms experienced by workers that were estimated to be associated with heavy metal toxicity were calculated in frequency. Anthropometrics, blood pressure, Hb levels, PCV and heavy metals levels were not significantly different between centers (p>0.05). Blood Pb and Cr from the SB and FA were significantly different from those at the SA center (p<0.05). There were no statistically significant correlations between blood Pb levels, blood Cr, and Pb waste with hemoglobin and PCV levels. Symptoms of skin irritation, dizziness, and joint pain were felt by 37-49% of respondents. Blood levels of Pb and Cr in the SB and FA centers are higher than in the SA centers. There was no significant correlation between Pb and Cr toxicity with hemoglobin and PCV levels. Symptoms of toxicity among batik workers are thought to occur due to chronic heavy metal toxicity due to the use of synthetic paint.KEYWORDS batik; haemoglobin; chromium; blood cell; plumbum
Karakteristik limbah dan dampaknya bagi kesehatan pembatik di Lendah, Kulon Progo Suhartini - suhartini; Sri Awalia Febriani; IBG. Surya Putra Pidada; Hendro Widagdo; Martiana Suciningtyas; Yudha Nurhantari; Dewanto Yusuf Priyambodo; Agus Surono; Roto Roto; Geraldine Nadita Putri Kinasih; Pramudji Hastuti; Sri Sutarni
Journal of Community Empowerment for Health Vol 2, No 2 (2019)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.42250

Abstract

Synthetic batik coloring often uses heavy metals element such as lead (Pb), chromium (Cr),silica (Si), and hydrochloric acid (HCl). The exposure of heavy metals in long period results in health impair on the artisans and eventually harming the environment. The purpose of this research is to analyze the waste characteristics and health impact towards the artisans in the Lendah, Kulon Progo. Seventy-six batik artisan, consisting of 37 from SB, 20 from FA, and 19 from SA underwent 5 ml blood sampling. The samples were used to determine their hemoglobin (Hb) level, packed cell volume (PCV), blood sugar, and total cholesterol. Blood plasma and wastewater were examined for Pb and Cr using the inductively coupled plasma (ICP) method; Si using atomic absorption spectrophotometry (AAS); and Cl- using spectrophotometry. Respondents underwent health checks and were interviewed about the impact of waste and disease complaints experienced using a questionnaire. Cognitive disorders were examined using mini-mental state examination (MMSE) and peripheral neuropathy was assessed using diabetic neuropathy symptom (DNS) score. The results obtained were analyzed by analysis of variance and t-test. Correlation test was used to examine the relationship of Pb and Cr with cognitive disorders. Total cholesterol levels and blood sugar were analyzed descriptively. There is no difference in knowledge about waste in batik makers in SB, FA, and SA. The level of Pb, Cr, and Cl- within the three production centers showed no significant difference (p > 0,05), whilst Si level showed significant difference between centers (p < 0,05). There is no correlation between Pb and Cr blood levels with cognitive impairing according to MMSE and occurence of peripheral neuropathy according to DNS score (p > 0.05). Normal cholesterol levels (< 200mg/dL) andnormal blood glucose levels (< 200 mg/dL) were observed in 70.0-80.5% and 85.0-95.1% batik artisans, respectively. Si levels in all three production centers’ waste shows significant difference. The most frequent complaint was back pain and dizziness. There is no correlation caused by Pb and Cr levels toward cognitive impairing. Cholesterol level in batik artisans tended to be high.
Co-Authors Agus Surono Dewanto Yusuf Priyambodo Dianandha Septiana Rubi Febriana, Sri Awalia Geraldine Nadita Putri Kinasih Hendro Widagdo Hendro Widagdo Hendro Widagdo I Gusti Wayan Murjana Yasa IBG. Surya Putra Pidada Ida Bagus Gd. Surya Putra Pidada Idha Arfianti Wira Agni Indra Sari Kusuma Harahap Kusumo Dananjoyo marsa, mardhatillah Martiana Suciningtyas Martiana Suciningtyas Mustofa Mustofa Neni Trilusiana Rahmawati Neni Trilusiana Rahmawati Ngadikun Ngadikun Pramudji Hastuti Prasetyastuti Roto Roto Sri Sutarni Sunarti Sunarti Tasmini Tasmini Tri Ratnaningsih Wikan Basworo Wiraagni, Idha Arfianti Yudha Nurhantari