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Identifikasi Isolat Mycobacterium bovis Dengan Konsentrasi Dna Bertingkat Menggunakan Teknik Polymerase Chain Reaction Yohanes T. R. M. R. Simarmata
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.1001

Abstract

Mycobacterium sp. is a bacteria that can cause tuberculosis disease in domestic animals. Mycobacterium bovis pathogens in 3rdrisk groups and Indonesia as the country's fourth-largest contributor of Tuberculosis in the world after India, China and South Africa. Based on these facts, the research was conducted to identify M. bovis with DNA stage concentrations, hope of the lowest concentration can be used as a reference for the detection of tuberculosis.Isolate DNA samples obtained from the Center for Veterinary Research (BBalitvet) in Bogor, West Java. Dilution of the DNA was started from concentration 5000 pg / mL; 2500 pg / mL; 1250 pg / mL; 625 pg / mL; 312.5 pg / mL; 156.25 pg / mL; 78.125 pg / mL and 39.0625 pg / mL . DNA amplification by PCR using two pairs of primers JB21 and JB22 Forward INS1 Reverse and Forward and Reverse INS2 with predenaturasi conditions 94 ° C for 5 min, denaturation 94 ° C for 1 min, annealing 60 ° C for 1 min, 72 ° C elongation for 1 min and post-elongation 72 ° C for 7 min at 35 cycles. PCR reaction products of 500 bp and 245bp.Analysis results can be seen in the concentration of 78.125 pg / mL and 39, 0625 pg / mL with primary INS1/INS2, bands (bands) starts to look faded and disappeared. PCR using primers JB21/JB22 showed that the concentration 39.0625 pg / mL bands had disappeared. This can be done as a source of reference for the detection of Mycobacterium bovis infection.
EFEK PENYUNTIKAN HORMON PREGNANT MARE’S SERUM GONADOTROPIN (PMSG) TERHADAP PENINGKATAN PRODUKTIVITAS INDUK BABI PADA PETERNAKAN BABI KOMERSIL DI KABUPATEN KUPANG Marianus Perada Kuslianto; Cynthia D. Gaina; Yohanes T. R. M. R. Simarmata
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1042

Abstract

Pigs are one of the potential livestock to breed with the aim of meeting the needs of animal protein, but in fact the level of pig production is not optimal due to the low efficiency of reproduction. One way that can be used to increase the productivity of sows that by superovulation method. This study aimed to determine the effects of PMSG hormone injections to increase the productivity level of sows that given superovulation treatment. 10 sows that have been selected, be divided into two research groups: control group and treatment group. Before the treatment is given in the form of PMSG hormone injections, sows from both study groups, first injected with hormones PGF2α to estrus synchronization purposes. Then, sows in the treatment group are injected by PMSG hormone with the dose of 2 ml/sow before naturally mating with the boar. After the mating process, the gestation period of pregnant sow will be observed until the parturition time. The parameters were litter size and the birth weight of piglets produced from each sows of the two study groups. Sows were injected with PMSG hormone before mating show better productivity levels compared with sows in the control group. Sows were injected with PMSG hormone produce the overall number of piglets are 46 piglets or the litter size are 9 piglets with the average birth weight of 1.20 kg, while in the control group generates total number of piglets are 36 piglets or the number of litter size are 7 piglets with the average birth weight of 1.05kg. Based on statistical tests, the results showed that there’s no significantly different ((P>0.05) for the litter size of each research groups. The results showed that injection of the PMSG hormone as a superovulation agent before mating is able to increasing piglet birth weight but not able to increase the litter size significantly. Increased productivity of sows through superovulation method is to extend the production period of the sow, especially sows with a number of birth five or more times without being replace by new gilt.
LAPORAN KASUS FASCHIOLOSIS PADA SAPI BALI DI DESA NOELBAKI, KECAMATAN KUPANG TENGAH, KABUPATEN KUPANG Yohanes T.R.M.R Simarmata; Lidya Aprilia Tjandring; Yovita Florida Bria Seran
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1597

Abstract

Fasciola sp. merupakan jenis parasit yang paling banyak menginfeksi sapi bali. Gejala klinis yang khas dari infeksi faschiolosis yaitu bottle jaw. Sapi bali betina berumur 6 tahun dengan berat badan seberat 200 kg di Desa Noelbaki dengan pemeriksaan fisik menunjukkan gejala klinis yakni edema pada submandibularis (bottle jaw), diare, nafsu makan baik, suhu tubuh 38.6oC dan Body Condition Score (BCS 3). Pemeriksaan laboratorium yakni pemeriksaan feses (TTGT) dan pemeriksaan darah lengkap. Hasil pemeriksaan mikroskopis sampel feses ditemukan telur cacing Fasciola sp dengan 500 TTGT. Pemeriksaan darah rutin menunjukan RBC 4,22 x 106/uL, Hb 6,8 g/L dan PCV 21,6 %. (lebih rendah dari normal). Tindakan terapi yakni pemberian antihelmentik (Oxfendazole 900 mg), ivermectin 1% sejumlah 0,04 mg dan antibiotik Procaine penicilin G, Dihydrostreptomycin Sulphate 4.000 mg. Pasca terapi, edema pada submandibularis mengalami penurunan, dan hasil pemeriksaan feses secara mikroskopis, jumlah telur cacing menjadi 100 TTGT.
LAPORAN KASUS : PYOMETRA PADA ANJING GOLDEN RETRIEVER Yohanes T. R. M. R. Simarmata; Anita Kartini Lakapu; I Dewa Made Anom
JURNAL KAJIAN VETERINER Vol 8 No 1 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i1.2265

Abstract

Pyometra in dogs is a condition of the accumulation of purulent material in the uterus of female dogs caused by hormonal imbalances resulting in bacterial infections and excess fluid production in the uterus. A female Golden Retriever named Merry, age 4 years old, having her address at Jln. Raya Dharmasaba Denpasar with complaints of a limp dog, anorexia, issued a thick reddish-colored discharge from the genitals and smelly. Based on the results of systematic clinical examination and the results obtained that the temperature of the dog is 38.1 ℃ (normal), pulsus frequency 120x/minute and the frequency of breathing 40x/ minute. At the time of inspection and palpation of the large abdomen, the nipple comes out. In a complete blood test conducted at Anom Animal Clinic showing the results of Leucosytosis (an increase in white blood cells) which indicates the incidence of bacterial infection, the dog is diagnosed with pyometra after being supported also by X-ray examination results showing radiopaque on the abdomen and ultrasound results showing the uterus anechoic. Dogs treated by surgery to remove the ovary and uterus (ovaryohysterectomy) and continued administration of antibiotics and analgesics. On the 9thday after surgery the incision wound had dried, the skin fused well, and was no longer removing the discharge from the genitals.
LAPORAN KASUS : PENANGANAN TRANSMISSIBLE VENEREAL TUMOR PADA ANJING LOKAL DI ANOM VET CLINIC Yohanes T. R. M. R. Simarmata; Margie P. Mila Meha; I Dewa Made Anom
JURNAL KAJIAN VETERINER Vol 8 No 1 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i1.2266

Abstract

Transmissible venereal tumor is a tumor that generally infects male and female genitalia and transmission occurs due to marriage and licking. On August 7th , 2019 the owner came with complaints of fresh blood dripping continuously from the vulva of Unyil, a local dog, female, approximately 3 years old, brown hair color, with a weight of 10.2 kg. After a physical examination in the perineal area, there is a mass like grapes formed in the vagina. Based on the results of physical examination and haematology test, the dog was diagnosed with transmissible venereal tumor. Handling is done in this case by removing the mass such as grapes formed in the vagina through surgery.
Service per Conception (S/C) dan Conception Rate (CR) Sapi Crossbreed (Persilangan Bos sondaicus dan Bos taurus) di Kecamatan Kupang Timur Tarsisius Considus Tophianong; Frans Umbu Datta; Tri Utami; Nancy Foeh; Cynthia Gaina; Yohanes Simarmata; Putri Pandarangga; Yeremia Y. Sitompul; Herlina Umbu Deta
JURNAL KAJIAN VETERINER Vol 11 No 1 (2023): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v11i1.10287

Abstract

Conception Rate (CR) and Service Conception (S/C) are two parameters for assessing the reproductive performance of cattle. This research was conducted to determine the value of S/C and CR in crossbreeding cattle in the working area of ​​the Public Health Center. The material used in this study was 72 crossbreed cattle aged 3-10 years. Semi-intensive rearing system with AI mating. The results the S/C in this study was 3.9 higher than the ideal standard of 1.6–2. The CR in this study was 58.6%. CR numbers are still relatively low from the ideal, namely 65 -75%.
IDENTIFIKASI STATUS REPRODUKSI SAPI AKSEPTOR (ISRA) PADA KELOMPOK TERNAK SETETES MADU DESA CAMPLONG II KECAMATAN FATULEU KABUPATEN KUPANG PROVINSI NUSA TENGGARA TIMUR Tarsisius Considus Tophianong; Yohanes T. R. M. R. Simarmata; Heny Nitbani
Jurnal Media Tropika Vol 1 No 1 (2021): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v1i1.3927

Abstract

East Nusa Tenggara (NTT) is one of the centers for beef cattle in eastern Indonesia, it has large areas and supportive natural potential. On the other hand, at Setetes Madu cattle group, in Camplong Village II, Fatuleu sub-district, Kupang Regency, East Nusa Tenggara Province, farming management and animal health management still traditionally maintains without planning and recording, as well as cattle rearing practice. Several problems of cattle farming development at Setetes Madu cattle group are, (1) unknown or unidentified of the individual reproductive health status of acceptor cattle; (2) minimum knowledge of farmers about cattle reproductive management includes breeding management and recording. The approaches to solving the problems are, (1) identification of the Reproductive Status of Acceptor Cattle to determine individual cattle reproductive health status and treatment to a cattle with the reproductive disorder; (2) educating cattle’s breeder about planned, recorded, measurable, and periodically evaluated and continuously of reproductive management. Without good reproductive management, efficiency reproductive will not be achieved, whereas, without reproductive efficiency, productivity will not be achieved. Reproduction performance and cattle health are important to achieve optimization, reproductive efficiency, profitability, and sustainable cattle breeding.
BUDIDAYA IKAN AIR TAWAR DI LAHAN KERING DESA CAMPLONG II KECAMATAN FATULEU KABUPATEN KUPANG, NTT Cynthia Dewi Gaina; Filphin Adolfin Amalo; Maxs. U. E. Sanam; Frans Umbu Datta; Inggrid Trinidad Maha; Yohanes T. R. M. R. Simarmata; Tri Utami
Jurnal Media Tropika Vol 1 No 1 (2021): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v1i1.3928

Abstract

Increasing the economy of rural communities is a process of improving an economic effort to meet the needs of life through an empowerment system form that meets human needs by utilizing surrounding natural resources. Natural resources owned by rural communities are the potential to be developed to improve the economy of the village community. If freshwater aquaculture is well developed and managed properly, it will bring beneficial results in line with the increase in fulfillment of public’ requirements. The purpose of community service program activities in Camplong II Village, Fatuleu District, Kupang Regency was community empowerment through freshwater aquaculture using the pond method. The people of Camplong II village have limited access to obtaining fresh fish products as its location is around 45-50 km from Kupang city which is known for its fresh sea products. Thus, the activities implemented were training on the installation of a square pond, providing 500 fish seeds and fish feed for 2 periods, mentoring and monitoring the maintenance of this pond, and developing human resources. The results obtained: installation of several fish pond with a diameter of 3 meters and a height of 4.0 m, an increase in community knowledge and skills, an early fish harvest stocked from this period which can improve the welfare of the community in Camplong II Village, Kupang Regency. From the results obtained there is potential in the form of land, water sources, and facilities that can be empowered to increase fish water farming production.
PENINGKATAN KUALITAS DAN KAPASITAS USAHA DENDENG BABI DI SIKUMANA, KOTA KUPANG Meity Marviana Laut; Larry R. W. Toha; Julianty Almet; Dewi Djungu; Yohanes Simarmata
Jurnal Media Tropika Vol 1 No 2 (2021): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v1i2.5742

Abstract

The small food processing industry in Kupang City has increased in recent years. One of the evolving food processing industries is pork processing. Pork jerky is one of the processed pork products and is classified as a new product for the community in Kupang City. When compared to se'i, pork jerky is not very popular among people in NTT. This can be because there are still very few UMKMs (Micro Small and Medium-sized enterprises/MSMEs) that produce pork jerky.UMKMs pork jerky Debali is one of the small business units in Kupang City that produce pork jerky. In addition to being marketed in Kupang City, Debali pork jerky products have also been marketed out of Kupang City. But like other UMKMs, Debali experiences production process problems such as a simple production process that takes a relatively long production time, the number of products is still insignificant, simple product packaging and vacuum packaging products are not available which have a longer shelf life. Community service activities to transfer science and technology (Ipteks) are carried out to improve the quality and capacity of Debali pork jerky business through counseling, gas ovens operation training as an alternative to drying jerky, packaging jerky with modern packaging and vacuum packaging, as well as assistance in marketing and business analysis.
Kondisi Populasi dan Habitat monyet ekor panjang (Macaca fascicularis) Claudya A. Dhaja; Yohanes T.R.M.R. Simarmata; Gerson Njurumana
Jurnal Veteriner Nusantara Vol 2 No 1 (2019)
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v2i1.1094

Abstract

This study investigated the long-tailed macaque (Macaca fascicularis) population and the habitat condition currently located in the Monkey Cave Recreational Park in Tenau. The long-tailed macaques in Monkey Cave Recreational Park in Tenau were originally brought by the colonialists of the Netherlands as pets. The population was dispersed during World War II when Japanese imperialists invaded Timor island. The habitat of the macaque in Tenau has since been improved by the Tourism Office in cooperation with Kupang City’s Central agency of Forest Area Consolidation. In 1998 a land area of 0.8 ha was allocated to form a habitat for the long-tailed macaque population. Since the formation of this protected ecosystem, there has not yet been any research on the population and habitat conditions of the macaques. Data and information related to these animals can be useful to support management plans, especially for primate-based ecotourism development. This study aimed to determine the structure of these populations as well as the habitat conditions of long-tailed macaques in Monkey Cave Recreational Park in Tenau.