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All Journal Partner Jurnal Pengabdian Masyarakat Peternakan Jurnal Ilmiah Inovasi Jurnal Ilmu Peternakan Terapan Journal of Applied Veterinary Science and Technology
Nugraheni, Yudhi Ratna
Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Social Sciences Veterinary Other

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Journal : Jurnal Ilmu Peternakan Terapan

 1 
Parasit Gastrointestinal pada Sapi di Daerah Aliran Sungai Progo Yogyakarta Yudhi Ratna Nugraheni; Dwi Priyowidodo; Joko Prastowo; Eryl Sri Rohayati; Ana Sahara; Aan Awaludin
Jurnal Ilmu Peternakan Terapan Vol 1 No 2 (2018): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

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Abstract

The aim of study was investigate gastrointestinal disease on cow fed forage from Progo watershed. Sixty three feses samples were taken from cows on Progo watershed. The method of feses analysis used was sentrifuge and Parfitt Bank. The results showed that 63 samples of cows feces were examined, among 25 were infected by Fasciola sp., 7 Paramphistomum sp., 14 Strongyle, and 9 Coccidiosis. The conclusion was the prevalence of gastrointestinal disease in cows was dominant incidence of Fasciolosis by 40%, Strongyle 22%, 14% Coccidiosis, and Paramphistomum sp.11%.
Studi Lapang: Penegakan Diagnosis Infectious Bursal Disease (IBD) Pada Ayam Broiler Aan Awaludin; Yudhi Ratna Nugraheni; Theo Mahiseta Syahniar; Dyah Laksito Rukmi; Agus Hadi Prayitno; Nurkholis Nurkholis
Jurnal Ilmu Peternakan Terapan Vol 3 No 1 (2019): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

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Infectious Bursal Disease (IBD), also called Gumboro, was disease which attacked cells in the bursa of fabricius, causing interference with the chicken's immune system or immunosuppressive. IBD was the one of viral disease that often attacks chickens in the field. The aim of this study was to determine the diagnosis of IBD by through at clinical symptoms and necropsy that can still be relevant on in the field. The diagnosis of IBD correctly, cheaply, easily and quickly in the field is very important to optimize the health care management and evaluation program. The method used was by observing clinical symptoms of broiler chickens in commercial farms suspected of contracting IBD and observing post-death organs (necropsy). The object of necropsy was 5 samples of broiler chickens from the farm. Data was analyzed descriptively. The results of this study was the broiler chickens that infected with IBD could be diagnosed through clinical symptoms and post-death organ changes, so that the diagnosis for IBD cases in the field used the observation of clinical symptoms and necropsy are still relevant.
Filogenetik Kutu Penghisap Darah (Haematopinus sp.) pada Beberapa Jenis Sapi berdasarkan Gen 18S rRNA Aan Awaludin; Yudhi Ratna Nugraheni; Mira Andriani; Niswatin Hasanah; Agus Hadi Prayitno
Jurnal Ilmu Peternakan Terapan Vol 3 No 2 (2020): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

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The purpose of this study was to determine the phylogenetic relationship of Haematopinus sp. which became parasites in several types of cattle, namely Simmental, Limousin, PO (Peranakan Ongole), and FH (Friesian Holstein) from Jember Regency and Simmental and PO cattle from Karanganyar Regency. The method used was to isolate DNA from 6 samples of Haematopinus sp. and amplified using 18S rRNA universal primers with 18S (5'-TCATTACGAGCTCTGCAAT-3) reverse primers and 18S (5'-TTCAAAGTAAACGTGTCGGC-3) sequential PCR sequencing. Sequencing results were analyzed using MEGA 6 software for phylogenetic tree construction using the Neighbor-Joining and Maximum Parsimony Methods. The results showed that the sample Haematopinus sp. originating from Simmental, Limousin, and PO cattle from Jember, and Simmental and PO from Karanganyar were included in one cluster by Haematopinus quadripertusus. Haematopinus sp. sample from Jember FH cattle had a considerable genetic distance from Haematopinus quadripertusus which was possible because the sequence that could be analyzed was only 236 nt.
Koksidiosis pada sapi perah di Kabupaten Jember, Jawa Timur – Indonesia Nur Muhamad; Aan Awaludin; Yudhi Ratna Nugraheni
Jurnal Ilmu Peternakan Terapan Vol 4 No 2 (2021): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

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This study aims to determine the incidence of coccidiosis in dairy cattle in the Jember District. A total of 75 samples were obtained from Friesian Holstein female dairy cows of varying ages. The materials used were fecal samples, saturated salt (saturated sodium chloride), and distilled water. The method used is a quantitative method with the Mc technique. Master and Whitlock to count oocysts per gram of stool (OPG) found in samples to determine the severity of coccidiosis. Stool samples are taken directly from the rectum of the dairy cattle or taken from the floor of the pen if the dairy cows have just released feces (fresh feces). The stool sample taken for each dairy cow is 10 grams of feces. Identification of the oocysts of Eimeria spp. done qualitatively by using the floating method or the flotation method. The data obtained were analyzed using descriptive analysis, then the prevalance was calculated. The results showed that the prevalence of coccidiosis in the Jember District reached 20% with a mild category of infection.
Investigation of tick-borne pathogen in goats, case study in Samigaluh, Kulon Progo, Yogyakarta Yudhi Ratna Nugraheni; Rochmadiyanto; Apinya Arnuphapprasert; Dwi Priyowidodo; Nur Muhamad; Sarwo Edy Wibowo
Jurnal Ilmu Peternakan Terapan Vol 6 No 2 (2023): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v6i2.3722

Abstract

Tick-borne pathogens of the genus Theileria and Anaplasma has been widely distributed and infect small ruminant in tropical and subtropical countries. Several species are considered highly pathogenic, while some cause subclinical infections in small ruminants. The distribution data of theileriosis and anaplasmosis in Samigaluh, Kulon Progo remain scarce and poorly understood. Therefore, further investigations are required to control this tick-borne disease. This study aimed to investigate the occurrence of theileriosis and anaplasmosis in domestic goats in Samigaluh, Kulon Progo, Western Yogyakarta. A total of 53 blood samples were collected from domestic goats. Blood sample was drawn from the jugular vein individually and kept in an EDTA tube. Thin blood smear was stained by Giemsa and observed under microscope for genus-level identification. As a result, it was found that theileriosis 12/53 (23%), anaplasmosis 25/53 (47%) and mixed infection 6/53 (11%) were detected in the present study.The conclusion of this study is that the incidence of Anaplasma sp. infection in goats is greater than Theileria sp. infection in Samigaluh, Kulon Progo.Although all domestic goats were asymptomatic, the finding of this study may shed light on the distribution of theileriosis and anaplasmosis infecting domestic goats in Samigaluh, Kulon Progo.
Detection of malaria in goats and sheep Yudhi Ratna Nugraheni; April Hari Wardhana; Aan Awaludin; Joko Prastowo; Surya Widyarsi; Yosua Lede Pono
Jurnal Ilmu Peternakan Terapan Vol 7 No 1 (2023): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v7i1.4372

Abstract

Plasmodium species, as the causative agent of malaria, are a significant concern in livestock production. This study aims to investigate the presence of malaria parasites in small ruminants comprised of goats and sheep. Plasmodium infections in ungulates do not cause malaria in humans. However, they might have anopheline mosquitoes as the same vector of this parasite. This study uses molecular techniques, nested PCR, to detect Plasmodium infections in small ruminants, as traditional microscopic examination may lead to misdiagnosis. The results show that Plasmodium infections were found in goat samples from Sumba Barat Daya, Nusa Tenggara Timur, with a prevalence of 7.41% based on nested PCR assay. Notably, Plasmodium was not detected through microscopic examination, underscoring the sensitivity of molecular techniques. It should be noted that none of the goats tested positive for malaria based on microscopic examination, suggesting extremely low parasitemia levels. This study emphasizes the importance of understanding Plasmodium infections in small ruminants, shedding light on their impact on animal health and their potential role in local transmission patterns. The sample size in this study is limited. Further research with larger samples and expanded geographical scope is recommended to comprehensively understand Plasmodium prevalence in small ruminants. 
Co-Authors . Dwilantiani Agus Hadi Prayitno Ana Sahara Anie Insulistyowati Apinya Arnuphapprasert April Hari Wardhana Awaludin, Aan Dwi Priyowidodo Dwi Priyowidodo Dyah Laksito Rukmi Eryl Sri Rohayati Fadel Muhammad Priyatna Fahmida Manin Joko Prastowo Kurniasih Kurniasih Mira Andriani Niswatin Hasanah Nur Muhamad Nur Muhamad Nurkholis, - Paradewa Baskara Raden Wisnu Nurcahyo Rochmadiyanto Sahara, Ana Sarwo Edy Wibowo Surya Widyarsi Theo Mahiseta Syahniar Winarsih, Sugi Yosua Lede Pono