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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Plumula : Berkala Ilmiah Agroteknologi Biotropika AGRIVITA, Journal of Agricultural Science JSMARTech : Journal of Smart Bioprospecting and Technology
Fatchiyah Fatchiyah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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PRODUCTION AND POTENCY OF LOCAL RAMBUTAN AT EAST JAVA AS A CANDIDATE PHYTOPHARMACA Lestari, Sri Rahayu; Djati, Muhammad Sasmito; Rudijanto, Ahmad; Fatchiyah, Fatchiyah
AGRIVITA, Journal of Agricultural Science Vol 35, No 3 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Rambutan is a tropical fruit that grow well in Indonesia and the peel is considered as waste. Many researchers’ showed that rambutan peel contains polyphenol that could be expected to avoid obesity.  The objective of this study was to explore the increasing production of local rambutan and to identify the promising phytochemical compounds on its peel as phytopharmaca candidate against obesity. Survey was conducted on the production of rambutan, potential plantation area, and marketing. Sample of rambutan peel collected from the sub-district Kanigoro, Blitar. Phytochemical compounds were analyzed using TLC, HPLC and FT-IR. Bioassay analysis used obesity rat models. The survey result showed a mean of rambutan production increased 2,6% in 2007-2012. Average production of rambutan 70-120 kg/tree. Vegetative multiplication usually done to maintenance of rambutan quality. The main compound of  Rambutan peel  extract (RPE) is flavonoids, tannins, ellagic acid and the major functional group of CH3, aliphatic CH3, and C=O. These compounds have a potential activity against obesity.  RPE 30 mg/kgBW dose was significantly inhibit the weight gain of obese rats and reducing the adipocyte size (p<0.05).Key words: potency, production, local rambutan, blitar, obesity
STUDI AWAL EMPAT ISOLAT BAKTERI ANTAGONIS TERHADAP JAMUR Colletotrichum gloeosporioides Elly Syafriani; Femy Riwany; Rahmi Handayani; Rahmi Kamelia; Istino Ferita; Fatchiyah Fatchiyah; Jamsari Jamsari
Plumula : Berkala Ilmiah Agroteknologi Vol 6 No 2 (2018): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.985 KB) | DOI: 10.33005/plumula.v6i2.9

Abstract

Anthracnose disease on some important crops is caused by the Colletotrichum gloeosporioides. Because of this disease, many farmers were suffer a financial loss. The conventional way to solve this problem that taken by the farmers is synthetic fungicides application which has a negative impact on consumers and the environment. Another alternative to replace it is needed. Antagonistic microorganisms can be used to replace it then and to develop a biofungicide which is more safety for the consumers and the environment. Four isolates of antagonist bacteria to against C. gloeosporioides have been found, namely: UBCR_12, UBCR36, UBCF_01, and UBCF_13. They were being developed as the main material for the manufacture of biofungicide. As an initial step of it, some tests were conducted to identify and to obtain some information that related to the character of all the isolates. Gram staining and KOH test showed that UBCR_12, UBCR_36, and UBCF_01 were gram negative bacteria and UBCF_13 is gram positive bacteria. The four isolates were also showed rod morphological form. Oxidative-fermentative (OF) test showed that the four isolates are facultative anaerobic bacteria. The protease enzyme activity test showed that only 3 of 4 isolates can produced a protease enzyme with the highest clear zone index to the lowest, respectively are UBCR_12, UBCF_13, and UBCF_01. This information leads to choosing UBCR_12 for the molecular identification by the cloning 16S rRNA gene of it. The result showed that UBCR_12 is Serratia plymuthica.
KAJIAN NUTRIGENOMIK: PENGHAMBATAN Igf-1 PADA ADIPOGENESIS JARINGAN LEMAK VISERAL TIKUS DENGAN EKSTRAK KULIT RAMBUTAN Rizky Nurdiansyah; Sri Rahayu Lestari; Fatchiyah Fatchiyah
Biotropika: Journal of Tropical Biology Vol 1, No 6 (2013)
Publisher : University of Brawijaya

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Abstract

Tujuan penelitian adalah untuk mengetahui dan menjelaskan pengaruh dari ektrak kulit buah rambutan terhadap adipogenesis jaringan lemak viserall tikus dengan melihat ekspresi igf-1. Tahapan penelitian yang dilakukan adalah ekstraksi kulit buah rambutan, perlakuan pada tikus, isolasi protein lemak viserall, separasi protein dan western blot. Tikus strain wistar jantan dibagi menjadi tikus berat badan normal dan obesitas. Perlakuan yang dilakukan antara lain kontrol, asam elagat, placebo, dan perlakuan ekstrak dengan dosis 5, 10, 15, dan 20 mg/kg berat badan. Hasil menunjukkan bahwa tren penurunan berat badan terjadi pada grup tikus obesitas dengan asupan pakan yang tidak berbeda signifikan (P>0,05). Dosis efektif terlihat pada perlakuan 10 mg/kg dan pada dosis 20mg/kg justru terjadi kenaikan berat badan untuk kedua jenis grup tikus. Profil protein antara tikus normal dan obesitas menunjukkan pola yang hampir sama dengan perbedaan intensitas tiap pita. Ekspresi igf-1 terpaut dengan ekspresi igfbp-1 pada 36,7 kDa dan terjadi pada seluruh perlakuan dengan perbedaan nyata pada kedua grup. Ekstrak kulit buah rambutan dengan dosis efektif 10mg/kg memiliki efek anti-obesitas pada tikus obesitas. Menariknya, ekspresi igf-1 menurun pada perlakuan 20mg/kg berat badan dibandingkan dengan kelompok lain. Hal ini diduga penghambatan obesitas tidak melewati jalur tyrosin kinase pada IR family.
Peran Alpha-S2 kasein Susu Kambing PE Terhadap Perbaikan Morfologi dan Histologi Membran Synovial Tikus Rheumatoid Arthritis Rivqi Rifa Bia; Fatchiyah Fatchiyah; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 2, No 5 (2014)
Publisher : University of Brawijaya

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Abstract

Penelitian ini bertujuan untuk menganalisa efek pemberian alpha-S2 kasein  susu kambing PE terhadap perubahan morfologi dan histologi membran sinovial tikus Rheumatoid Arthritis (RA). Tahapan penelitian meliputi pembuatan RA menggunakan multidosis CFA (Complete Freud Adjuvant. Kelompok perlakuan dibagi menjadi (C: Kontrol; CM: Kontrol + alpha-S2 kasein; RA: Rheumatois Arthritis; RAM: Rheumatois Arthritis + alpha-S2 kasein). Treatment RA dilakukan  selama 2 bulan dengan dosis 2mg/kg. Analisa morfologi dilakukan dengan skoring perubahan bentuk kaki dan histologi menggunakan metode HE yang divisualisasi menggunakan mikroskop BX53. Hasil penelitian menunjukkan adanya perbaikan morfologi yang ditandai dengan penurunan hasil skor klinis (C = 0; RA = 4; CM = 0; RAM = 1.3). Perbaikan histologi terletak pada penurunan jumlah hiperplasia pada kelompok RA + alpha-S2 kasein menuju kondisi normal dimana penurunan level tersebut mengidikasikan alpha-S2 kasein dapat digunakan sebagai alternatif penyembuhan RA.
Virtual Inhibition Analysis of Bioactive Compound Brazilin (Caesalpinia sappan L.) Toward Progesterone Receptor or Lonaprisan in Breast Cancer Proliferation Ayu Puspitaning Dewi Harnis; Nur A.H.M. Hasan; Yuni K. Janah; Chaidila A. Tsamara; Fatchiyah Fatchiyah
Biotropika: Journal of Tropical Biology Vol 8, No 2 (2020)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2020.008.02.01

Abstract

Breast cancer is one of the leading causes of death worldwide. The pathway of breast cancer in KEGG shows that the most effective pathway is through the progesterone receptor (PR). Brazilin is a bioactive compound of secang (Caesalpinia sappan L.) used to inhibit breast cancer through survivin and Bcl-2 pathway but the interaction with PR route is unknown. This research was conducted to determine the virtual interaction between brazilin and PR and its comparison with lonaprisan, so the potential of breast cancer drugs that can overcome through three targets at once with minimal side effects is expected to be known. There are five docking interactions, including the interaction of PR-progesterone, PR-brazilin, PR-brazilin-progesterone, PR-lonaprisan, and PR-lonaprisan-progesterone. Protein and ligand preparation was performed by using Discovery Studio Client 2019 and PyRx 0.8, molecular docking was performed by using Hex 8.0.0 and visualization used Discovery Studio Client 2019. Virtual interaction results shows that lonaprisan has the most stable bond (lowest binding energy), -333.8kJ/mol but when progesterone was docked afterwards the result shows the opposite. Brazilin has a more stable bond compared to lonaprisan with a difference of 2.1kJ/mol and supported by hydrophobic bonds also capable of changing the position of progesterone in binding to PR so that it is estimated that brazilin has the potential as SPRMs, an alternative breast cancer drug to replace lonaprisan. Herbal medicine with brazilin can be estimated to fight breast cancer through 3 targets at once (survivin, Bcl-2, PR).
Optimization of Neuron cells Maturation and Differentiation Choirunil Chotimah; Fatchiyah Fatchiyah
Biotropika: Journal of Tropical Biology Vol 2, No 4 (2014)
Publisher : University of Brawijaya

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Abstract

Penyakit neurodegenerasi adalah penyakit yang menyerang sel otak dan sumsum tulang belakang dengan mematikan selnya. Salah satu penyakit neurodegenerasi adalah HAD yang prevalensinya sekarang meningkat sekitar 37 %. Oleh karena itu, peneliti mengembangkan penelitian untuk mencari obat yang dapat mencegah atau mengobati penyakit ini, salah satunya dalam bidang kultur jaringan dan sel hewan. Tujuan penelitian ini adalah untuk menganalis waktu optimal pertumbuhan kultur neuron otak tikus (Rattus norvegicus) dan tahapan diferensiasi morfologi sel neuron otak tikus. Penelitian ini dilakukan dengan cara mengisolasi sel neuron fetus tikus yang berumur 18-19 hari dan ditumbuhkan secara in vitro (MEM+10%FBS+3%Penstrep). Hasil yang didapatkan adalah pertumbuhan sel neuron yang optimal terdapat pada hari kedelapan. Diferensiasi morfologi sel neuron dimulai pada hari ke-1 dengan morfologi sel berupa neuroblast apolar (berbentuk bulat), hari ke-2 morfologi sel neuron berupa neuroblast bipolar (sudah terbentuk akson dan dendrit), pada hari ke-3 sampai hari ke-14 sel sudah matang, dengan morfologi sel yaitu neuron bipolar, piramidal dan multipolar. Sel neuron yang matang mengalami optimalisasi pada hari ke-10.Kata kunci : diferensiasi, kultur neuron, morfologi neuron, pertumbuhan sel neuron.
PRODUCTION AND POTENCY OF LOCAL RAMBUTAN AT EAST JAVA AS A CANDIDATE PHYTOPHARMACA Sri Rahayu Lestari; Muhammad Sasmito Djati; Ahmad Rudijanto; Fatchiyah Fatchiyah
AGRIVITA, Journal of Agricultural Science Vol 35, No 3 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i3.390

Abstract

Rambutan is a tropical fruit that grow well in Indonesia and the peel is considered as waste. Many researchers’ showed that rambutan peel contains polyphenol that could be expected to avoid obesity.  The objective of this study was to explore the increasing production of local rambutan and to identify the promising phytochemical compounds on its peel as phytopharmaca candidate against obesity. Survey was conducted on the production of rambutan, potential plantation area, and marketing. Sample of rambutan peel collected from the sub-district Kanigoro, Blitar. Phytochemical compounds were analyzed using TLC, HPLC and FT-IR. Bioassay analysis used obesity rat models. The survey result showed a mean of rambutan production increased 2,6% in 2007-2012. Average production of rambutan 70-120 kg/tree. Vegetative multiplication usually done to maintenance of rambutan quality. The main compound of  Rambutan peel  extract (RPE) is flavonoids, tannins, ellagic acid and the major functional group of CH3, aliphatic CH3, and C=O. These compounds have a potential activity against obesity.  RPE 30 mg/kgBW dose was significantly inhibit the weight gain of obese rats and reducing the adipocyte size (p<0.05).Key words: potency, production, local rambutan, blitar, obesity
BZLF1 Expression of EBV is correlated with PARP1 Regulation on Nasopharyngeal Carcinoma Tissues wahyu nur laili fajri; Ahmad Rofi'i; Fatchiyah Fatchiyah
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Nasopharyngeal carcinomas (NPC) is a cancer that arises in the epithelial tissue that covers the inside of the nasopharyngeal mucosa and nasopharynx. Infected Epstein Barr Virus (EBV) cell in a latent infection associated with the expression of nine latent proteins. Latent Membrane Protein 1 (LMP1) is one of latent proteins, and mayor EBV oncoprotein, with functions including virus growth, and to activate BamHI-Z Leftward Reading Frame 1 (BZLF1)-EBV, which can inhibit p53 to induce apoptotic resistance, metastasis, and immune modulation. The body will respond to the expansion of EBV infection with activation of Poly(ADP-ribose)Polymerase-1 (PARP1). The objective of study is to observe the expression of BZLF1 and determine PARP1 regulation in nasopharyngeal tissues. NPC-T2, NPC-T3 and polyp tissues slides are from Ulin Hospital, Banjarmasin. To characterize the necrotic cells such as pyknosis, karyorrhexsis, and karyolysis, histological slides were stained by HE that the necrotic cells measured by using a BX-53 microscope (Olympus) with CellSens Standard software. Tissues slides were stained by using immunofluorohistochemistry with EBV-BZLF1 antibody-Mouse anti-EBV monoclonal antibody against Goat anti-mouse IgG-FITC and anti-PARP1 antibody (MC-10) against Goat anti-mouse IgG labeled Rhodamin. The expression intensities were measured by Confocal Laser Scanning Microscope (Olympus). The percentage number of necrotic cells and BZLF1 and PARP1 expression intensity were analyzed using SPSS 16.0 by one-way ANOVA test with α = 0.05, beside that we use correlate and regression analyze. The research showed that the amount of karryorhexis higher than pyknosis and karyolysis in both tissues. BZLF1 expression 1.79 INT/sel (in polyp), 2.76 INT/sel (NPC Type 2) and 4.36 INT/sel (NPC Type 3), PARP1 expression 2.25 INT/sel (in polyp), 3.31 INT/sel (NPC Type 2), dan 5.93 INT/sel (NPC Type 3).The high of intensity of expression BZLF1 induced the increasing of PARP1 expression, but not correlated with percentage of necrotic cell. Interestingly, in all tissues (NPC-T1, NPC-T2 and polyp) is increased number of karyolysis cells properly, but not in pyknosis and karryorrhexis cells. The conclusion of this study indicated that the BZLF1 induce PARP1 to repair DNA damage against EBV infection.Keywords: BZLF1, CLSM, EBV, nasopharyngeal carcinoma, PARP1.
Phylogenetic construction of Green Algae Based on the rbcL Gene Aliarani Rosyidiana Putri; Galuh Arkana; Haikal Ageng Maulana; Septhyanti Aprilia Kavitarna; Rayhan Yugo Nurul Maulana; Muhammad Wisam Wira Sakti; Turhadi Turhadi; Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 1 (2023): JSMARTech Volume 4, No. 1, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.01.25

Abstract

Chlorophyta (green algae) is one of the algae that can be found in (both oceans and freshwater lakes) as well as land with temperate to dry climates. Chlorophyta or green algae can be found in oceans, freshwater lakes, temperate land, and dry climate land.  In addition, chlorophyta can also be found in semi-aquatic environments, such as rocks, moist soil, and moist tree bark. This study aims to analyze the phylogenetic relationship among chlorophyta species based on rbcL gene to classify the diversity of green algae in Indonesia. The green algae gene rbcL sequence with 20 species was obtained from NCBI. The sequences obtained were aligned with ClustalW and the mutation patterns were analyzed. The aligned sequences were made phylogenetic trees by MEGA-X with the Maximum Parsimony method and 1000 bootstrap. The total percentage of transversions that occurred was 10.75%, while the percentage of transitions was 7.85%. The construction of the phylogenetic tree shows that the chlorophyta species are grouped based on their habitat in the same clade. Clad 1 consists of genera Chlorella, Coccomyxa, and Volvox with freshwater habitats and a genus of Parachlorella with soil habitat. Clade 2 consists of genera Chlorococcum, Tetradesmus, Crucigenia, and Desmodesmus with freshwater habitats and a genus of Chlorococcum with soil habitats. Clad 3 consists of genera Ulva and Chlorella salina with marine habitat. The furthest pairwise distance analysis results were shown by Coccomyxa subellipsoidea and Sargassum schnetteri with a value of 1.18902. The kinship of the species of the Chlorophyta division based on the rbcL gene is divided into 3 clade separated by habitat. The phylogenetic tree in the Chlorophyta division shows that the rbcL gene is sensitive enough to separate among genera and can be utilized in phylogenetic studies.
Front Matter JSMartech Vol.04., No.02 Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.02.38

Abstract

Co-Authors Ahmad Rofi'i Ahmad Rudijanto Aliarani Rosyidiana Putri Aris Soewondo Ayu Puspitaning Dewi Harnis Chaidila A. Tsamara Choirunil Chotimah Elly Syafriani Femy Riwany Galuh Arkana Haikal Ageng Maulana Istino Ferita Jamsari Jamsari Moch Sasmito Djati Muhammad Wisam Wira Sakti Nur A.H.M. Hasan Rahmi Handayani Rahmi Kamelia Rayhan Yugo Nurul Maulana Rivqi Rifa Bia Rizky Nurdiansyah Septhyanti Aprilia Kavitarna Sri Rahayu Lestari Turhadi Turhadi wahyu nur laili fajri Yuni K. Janah