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All Journal Jurnal Hortikultura Indonesia (JHI) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal HPT (Hama Penyakit Tumbuhan)
Retno Dyah Puspitarini
Jurusan Hama Dan Penyakit Tanaman, Fakultas Pertanian, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Effects of Compost on Nematode Pratylenchus sp. (Tylenchida: Pratylenchidae) Population in Patchouli Subarjah, Cecep; Himawan, Toto; Puspitarini, Retno Dyah
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.07

Abstract

Patchouli is seen as one of the most important export commodities in Indonesia. The presence of parasitic nematode Pratylenchus sp. creates a barrier to plant cultivation. Increasing organic content in soil by applying compost in the plantation is one of approaches in controlling the nematode. This research was mainly aimed to study the effects of compost fertilization on the population growth of nematode Pratylenchus sp. and yields on patchouli. These research used a Completely Randomised Design (CRD) consisting of 12 treatments with three replicates. Selected plant was inoculated with nematodes with the following initial population: 100, 200, 300, 400, and 500 nematodes in soil with and without compost. The plant with no inoculation was used as control. The result of the research confirmed that the addition of compost was capable of suppressing the population of nematode Pratylenchus sp. as much as 58.93 - 67.31% and 40.74 - 59.19% in root and in soil of each treatment, respectively. Compost applied in each treatment positively affected the increasing amount of Patchouli oil yielded, and it could also reduce the loss as much as 18.34 - 43.36%. Trichoderma sp. was found to be dominant in compost in the treatment with its population of 2.1 × 104 propogul/g.
Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Pramudi, Muhammad Indar; Puspitarini, Retno Dyah; Rahardjo, Bambang Tri
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Muhammad Indar Pramudi; Retno Dyah Puspitarini; Bambang Tri Rahardjo
Journal of Tropical Life Science Vol. 3 No. 3 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
Effects of Compost on Nematode Pratylenchus sp. (Tylenchida: Pratylenchidae) Population in Patchouli Cecep Subarjah; Toto Himawan; Retno Dyah Puspitarini
Journal of Tropical Life Science Vol. 6 No. 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.07

Abstract

Patchouli is seen as one of the most important export commodities in Indonesia. The presence of parasitic nematode Pratylenchus sp. creates a barrier to plant cultivation. Increasing organic content in soil by applying compost in the plantation is one of approaches in controlling the nematode. This research was mainly aimed to study the effects of compost fertilization on the population growth of nematode Pratylenchus sp. and yields on patchouli. These research used a Completely Randomised Design (CRD) consisting of 12 treatments with three replicates. Selected plant was inoculated with nematodes with the following initial population: 100, 200, 300, 400, and 500 nematodes in soil with and without compost. The plant with no inoculation was used as control. The result of the research confirmed that the addition of compost was capable of suppressing the population of nematode Pratylenchus sp. as much as 58.93 - 67.31% and 40.74 - 59.19% in root and in soil of each treatment, respectively. Compost applied in each treatment positively affected the increasing amount of Patchouli oil yielded, and it could also reduce the loss as much as 18.34 - 43.36%. Trichoderma sp. was found to be dominant in compost in the treatment with its population of 2.1 × 104 propogul/g.
Co-Authors Agung Setyo Budi Agung Setyo Budi Akhmad Rizali Aminuddin Afandhi Aminuddin Afandhi Aminudin Afandhi Aminudin Afandhi Aminudin Afandhi Aminudin Afandhi Aminudin Afandhi Arif Danu Satria Ayu Kurnia Puspasari Bambang Tri Rahardjo Bambang Tri Rahardjo BANDUNG SAHARI Cecep Subarjah Desti Mega Pratiwi Evi Masfiyah Evi Masfiyah Fernando, Ito Firdausi Indah Lestari Fitriani, Ima Gatot Mudjiono Gatot Mudjiono Hagus Tarno Hosainul Basri Ika Putri Utami Karina, Anisya Putri Ludji Pantja Astuti M. Zuhran Mega Apriliyanti Mochammad Syamsul Hadi Muhammad Anton Astoni Muhammad Indar Pramudi Nurul Qomariyah Nurul Qomariyah Otto Endarto Pamungkas, Bayu Aji Rina Rachmawati Rina Rachmawati Salamah, Luklu Is Sigit Rahmansah Sigit Rahmansah Sri Karindah Sri Karindah Sri Karindah Subarjah, Cecep Syib’li, Muhammad Akhid Toto Himawan Toto Himawan Vivi Renna Pratiwi Yogo Setiawan Yulianto Nugroho Yulianto Nugroho Yusran Baddu Yusran Baddu Zuhran, M.