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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Penelitian Pascapanen Pertanian AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Agrin : Jurnal Penelitian Pertanian Biofarm : Jurnal Ilmiah Pertanian
Sriani Sujiprihati
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Economics, Econometrics & Finance

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Development of SCAR Marker for Detection of Sex Expression in Papaya (Carica papaya L.) from Several Genetic Backgrounds , Sobir; Sriani Sujiprihati; Evalina C. Pandia
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.875 KB) | DOI: 10.24831/jai.v36i3.1382

Abstract

Papaya plants are hermaphrodite, pistillate, or staminate. Sex inheritance in papaya is determined by a single gene locus with three alleles of M which is dominant for maleness, MH for hermaphrodites and m which is recessive for femaleness. Only fruits from hermaphrodite plants are marketed since they have the necessary commercial characteristics, i.e., they are pear-shaped and have thicker flesh and a smaller internal cavity. Increased papaya yield has been limited mainly by the ratio of female to hermaphrodite (1: 2) plants normally occurring in orchards. This ratio causes great losses to papaya producers. Identification of seedlings sex during nursery stage is of prime iportance. In order to obtain simple DNA markers to identify sex expression in papaya, five SCAR markers of 20-21 primers were utilized. Examination of these markers into 24 genotypes of papaya from 12 populations of different genetic background revealed that pair of primer PKBT-5 had successfully differentiated male and hermaphrodite plants from female plants. Hence, PKBT-5 pair of primer can be utilized as DNA marker for sex expression character identification in papayas.   Key words: Papaya, sex expression, SCAR marker
The Study of Genetic Diversity and Relationships on Carica sp. by Means of Random Amplified Polymorphic DNA (RAPD) Analysis Ahmad Satori; Sriani Sujiprihati; , Sobir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 1 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.417 KB) | DOI: 10.24831/jai.v30i1.1425

Abstract

The genus of Carica comprises 21 species, among them three species have been domesticated and cultivated. They are Carica papaya, Carica candamarcencis and Carica monoica. In Indonesia, two species have been cultivated viz. Carica papaya and Carica candamarcencis. In this study, four accessions of Carica papaya species and one accession of Carica candamarcencis species were analyzed using RAPD technique. We successfully amplified a total I of 40 fragments from these DNA genome by using 6 random primers with GC bases content:::: 60% of each primer. The number of fragments of each primer ranged from 5 to 8 averaged 6.7 fragments per primer. Out of total fragments, 90% showed as polymorphic ranged from 5 to 8 on average 6 fragments per primer. A dendogram based on the UPGMA- link method using Nei and Li similarity and Principal Component Analysis (PCA) plot separated the accessions into two main groups, the Carica papaya species on the one side and the Carica candamarcencis species on the other side. The result demonstrated that RAPD analysis was able to reveal genetic difference between Carica papaya and Carica candamarcencis species, as well as genetic diversity in Carica papaya species.
Perakitan Varietas Hibrida Jagung Manis Berdaya Hasil Tinggi dan Tahan Terhadap Penyakit Bulai Sriani Sujiprihati; Muhamad Syukur; Andi Takdir Makkulawu; R Neny Iriany
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 3 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.909 KB)

Abstract

Sweet corn is a result of recessive mutation which occurs naturally in gene that controls the conversion of sugar into starch in corn seed endosperm. There are 3 main genes that affect corn sweetness, namely sugary gene (su), sugary enhancer gene (se), and shrunken gene (sh2). This study aims to obtain a general combining ability value for the sweet corn lines, which will be the parent in diallel crosses; obtain the information of general combining ability, specific combining ability, and heterosis value for sweet corn lines; and obtain one or more promising single cross hybrids that will be potential for high yield and resistance to downy mildew disease. The study was conducted from April 2009 until November 2011 at the Experimental Field Centre for Cereal Plant Study (BALITSEREAL), Maros, South Sulawesi. The results showed that five genotypes had moderately high yield: Mr4/SC/BC4-3-1B, Mr14/SC/BC4-6-1B, Mr11/SC/BC4-3-1B, Mr14/SC/BC3-8-1B, and Mr12/SC/BC4-6-1B. The attack level of downy mildew that was classified as resistant has 0 to 7% rate. The combination of crosses that produce better productivity than all the varieties are: C × A (Mr11/SC/BC4-2-1B-1 × Mr12/SC/BC4-5-B-1), A × D (Mr12/SC/BC4-5-B-1 × Mr11/SC/BC4-2-1B-1), E × B (Mr12/SC/BC3-3-1B -1 × Mr14/SC/BC4-6-1B-1), and B × D (Mr11/SC/BC4-2-1B-1 × Mr14/SC/BC4-6-1B-1 ). Five hybrids can be continued for the preliminary testing of yields. Twelve candidate varieties do not have yield different than the checked varieties, so it can proceed to a multilocation trials to explore the advantages that can be released as new varieties.
Studi Karakter Mutu Buah Pepaya IPB Ketty Suketi; Roedhy Poerwanto; Sriani Sujiprihati; , Sobir; Winarso D.Widodo
Jurnal Hortikultura Indonesia Vol. 1 No. 1 (2010): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1675.89 KB) | DOI: 10.29244/jhi.1.1.17-26

Abstract

ABSTRACTThe objective of the experiment was to investigate the physical and chemical characteristic of eight genotypes of papaya i.e. IPB1, IPB 2A, IPB 3, IPB 3A, IPB 4, IPB 7, IPB 8, and IPB 9 on two stages of ripening period based on percentage of the yellow area of fruit peel (75% yellow and 100% yellow). The fruits were picked at 25% yellow of fruit peel colour. The experiment was conducted in split plot wi th completely randomized block design. The main plot was ripening periods of 75% and 100% ripe, while the genotypes were taken at subplot. There was no significant different on physical and chemical characteristics between papaya at stadium 75% and 100% yellow. Flesh firmness of IPB 9 was better than IPB 1, IPB 4 and IPB 8. Ascorbic acid content of IPB 4 (107.36 mg/100 g) was higher than that of IPB 2A and IPB 3A. Carotenoid content of IPB 4 (29. 73 mg/100g) was higher than that of the other genotypes.Key words: Carica papaya, physical characteristic, chemical characteristic, ascorbic acid, carotenoid
Pendugaan Komponen Ragam, Heritabilitas dan Korelasi untuk Menentukan Kriteria Seleksi Cabai (Capsicum annuum L.) Populasi F5 Muhamad Syukur; Sriani Sujiprihati; Rahmi Yunianti; Khaerin Nida
Jurnal Hortikultura Indonesia Vol. 1 No. 2 (2010): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1263.198 KB) | DOI: 10.29244/jhi.1.2.74-80

Abstract

ABSTRACTInformation on genetic variability and correlation between quantitative characters with yield are important for support the selection program. The objective of the research was to estimate the genetic variability, heritability, and path analysis on agronomic characters to determine the selection criteria in the chili. This research was conducted at Research Station of Leuwikopo, Darmaga, Bogor from November 2009 until May 2010. This research observed all populations i.e. F5 population: 320 plants, IPB C2 population: 20 plants, and IPB C5 population: 20 plants. The results showed that total fruit weight, thick and fruit diameter, middle fruit diameter, blossom end fruit diameter, fruit weight, and days to flowering have a high broad sense heritability . High coefficient of genetic variability values were obtained ini number of fruits per plant, fruit wei ght, stem diameter, fruit diameter, and fruit weight. Based on the heritability, genetic variability, correlations analysis and path analysis, characters that can be used as selection criteria in this study is the number of fruit per plant, fruit weight, and fruit diameter.Key words: genetic variability, heritability, path analysis, selection
Aplikasi Asap Cair dan Gel Lidah Buaya (Aloe Vera L.) Untuk Memperpanjang Masa Simpan Buah Pepaya Slamet Budijanto; Sriani Sujiprihati; Dini Rizkyah; Sulusi Prabawati
Jurnal Penelitian Pascapanen Pertanian Vol 8, No 1 (2011): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v8n1.2011.11-18

Abstract

Penelitian ini bertujuan untuk mempelajari pengaruh penggunaan anti fungi asap cair dan pelapisan gel lidah buaya dalam memperpanjang masa simpan buah pepaya. Penelitian dilakukan dengan rnenggunakan Rancangan Kelornpok Lengkap Teracak (RKLT) dua faktor. Faktor pertama perlakuan perendaman asap cair dan faktor kedua perlakuan pelapisan gel lidah buaya. Hasil penelitian perlakuan asap cair dapat menekan laju respirasi sedangkan perlakuan lainnya tidak dapat menghambat laju respirasi. Semua perlakuan tidak menurunkan susut bobot, dan padatan total terlarut (PTT). Perlakuan yang diberikan dapat memperpanjang masa simpan selama dua sampai tiga hari, dan dapat memperlambat pelunakan buah pepaya. Perlakuan asap cair, pelapisan gel lidah buaya dan kombinasi asap cair dan pelapisan gel lidah buaya sangat berpengaruh pada kesukaan panelis dalam menilai penampilan, aroma, rasa, tekstur dan warna daging buah pepaya. Buah pepaya yang diberi perlakuan pelapisan gel lidah buaya mempunyai mutu sensori yang lebih baik. . Liquid Smoke and Aloe vera Gel Application for Extending Shelf Life of PapayaThe aim of this research was to study the effect of liquid smoke and Aloe vera gel in extending shelf life of papaya fruit. This study used Randomized Complete Block Design (RCBD) with two factors. The first factor was liquid smoke. The second factor was of coating (C). comprising: without coating (Co) and with coating using Aloe vera gel (Cl). The result showed that liquid smoke treatment could suppressed the respiration rate significantly that the others could not. All of treatment did not influence to fruit weight loss and total solute solid. However, these treatments significantly extended self life for two to three days and delayed fruit softening. Moreover, the sensory analysis revealed that liquid smoke treatment resulted in significant fruit performance Improvement, and Aloe vera gel treatment significantly enhanced fruit flavor, smell, texture and color. Consumers preferred papaya fruit with Aloe vera gel coated treatment.
NONPARAMETRIC STABILITY ANALYSIS OF YIELD FOR NINE CHILI PEPPER (Capsicum annuum L.) GENOTYPES IN EIGHT ENVIRONMENTS Vitria Puspitasari Rahadi; Muhamad Syukur; Sriani Sujiprihati; Rahmi Yunianti
AGRIVITA Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.273

Abstract

 The objectives of this study were to compare nonparametric stability measures, and to identify promising high yield and stability of chili pepper (Capsicum annuum L.) genotypes in eight environments. In every environment, a Randomized Complete Block Design was used with three replications. The method of Nassar and Huehn, Kang, Fox, and Thennarasu was used to analyze the stability and high yield. Spearman’s correlation and Principal Component analysis distinguishes the methods based on two different concepts of stability: the static (biological) and dynamic (agronomic) concepts. The top method was found to be the dynamic stability. Meanwhile, the methods of Si1, Si2, Si3, Si6, Npi1, NPi2, NPi3 and NPi4 were found to be the static stability. Based on the ranking frequency stability of the nonparametric method, the genotypes with the highest frequency of static stability ranking were genotypes IPB002003, IPB002046, IPB009019 and Tit Super, whereas IPB009002 and Tombak were categorized as those of dynamic stability. Genotype IPB120005 and IPB019015 were less adaptable in the multiple environments tested. It shows that the genotypes were specific in certain environments. IPB120005 had high yield and specific location in Boyolali in dry season and IPB019015 genotype was specific in Bogor in wet season. Keywords: chili pepper, nonparametric stability, high yield, dynamic stablility
PEWARISAN KARAKTER KETAHANAN TERHADAP CHIVMV (CHILLI VEINAL MOTTLE VIRUS) PADA TANAMAN CABAI Zahratul Millah; Sriani Sujiprihati; Sri Hendrastuti Hidayat
Jurnal Agroekoteknologi Vol 4, No 1 (2012)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.076 KB) | DOI: 10.33512/j.agrtek.v4i1.620

Abstract

Inheritance of resistance trait to ChiVMV was studied in intraspecific capsicum population derived from a cross between two Capsicum annuum line, PBC495 as a resistance line and ICPN12#4 as a susceptible line. The resistance was assessed by diseased index (DI) and score of absorbance value at  405 nm, two weeks after inoculation. Based on t-test of F1 and F1R, it was concluded that there was no maternal effect. Nil symptoms resembling the resistant parent were identified in the progeny F2, BC1P1 (BC1 to the resistant parent) and BC1P2 (BC1 to the susceptible parent) populations. Segregation of resistance (nil DI and lower absorbance value) and susceptibility in the F2 fitted a 3:1 Mendelian ratio, indicating that resistance were controlled by a single dominant nuclear gene. Nil segregation of the trait in the test crosses in BC1P1 and a 1:1 ratio segregation in BC1P2 also confirmed the 3:1 gene segregating model as found in the F2. Heritability values of the trait were medium to high.Key words : inheritance, resistance, ChiVMV, pepper
INTERAKSI GENETIK X LINGKUNGAN UNTUK KETAHANAN CABAI ( Capsicum annuum L.) TERHADAP ANTRAKNOSA YANG DISEBABKAN OLEH Colletotrichum acutatum Muhammad Syukur; Sriani Sujiprihati; Jajah Koswara; Widodo .
Biofarm Jurnal Ilmiah Pertanian Vol 13, No 9 (2010): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v13i9.275

Abstract

Appearance of a plant is determined by genetic factors, environmental factors and interactions between them. The genetic x environment interactions become an important concern for breeders, in addition to genetic factors. Purpose of this study was to examine the genetic x environment interactions for resistance of pepper to anthracnose. Materials used were 16 hybrids that were planted at three locations. Complete randomized block design (RCBD) was used with three replications. Replicates nested within location. Twenty of hot pepper that has been worn but is still green from each replication was inoculated with C. acutatum, PYK 04 isolate. Disease incidence was observed five days after inoculation. The results showed that the genotype x location interaction was significant different to resistance resistance caused by Colletotrichum acutatum PYK 04 isolate. Pepper genotypes stable in anthracnose resistant character in three selection environments was IPB CH3, IPB CH6, and IPB CH25. The genotypes were suitable for selection environment Ciherang, Leuwikopo and Tajur. Genotype IPB CH50 and IPB CH51 were suitable for selection environment Tajur. Imperial genotypes was suitable for the environment selection Ciherang. IPB CH5 and IPB CH4 CH5 were suitable for Lewikopo environment.Keywords:genetic x environmental interaction, anthracnose, resistance, pepper, Colletotrichum acutatum
PENDUGAAN DAYA GABUNG DAN HETEROSIS KARAKTER HORTIKULTURA CABAI (Capsicum annuum L.) Agus Riyanto; Sriani Sujiprihati; Sri Hendrastuti H
Agrin Vol 12, No 2 (2008): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2008.12.2.87

Abstract

Penelitian ini bertujuan untuk informasi tentang nilai Daya Gabung Umum (DGU), nilai Daya GabungKhusus (DGK) dan nilai heterosis karakter hotikultura Capsicum annuum.. Percobaan ini menggunakan 21genotip yang terdiri atas 6 tetua dan 15 hibridanya. Rancangan acak kelompok lengkap dengan tiga ulangandigunakan dalam penelitian ini. Hasil penelitian menunjukkan bahwa berdasarkan pendugaan daya gabungkhusus, nilai heterosis dan nilai tengah karakter maka 12 hibrida berpotensi untuk dijadikan varietas hibridaberdaya hasil tinggi. Kedua belas hibrida tersebut adalah IPB C2 x IPB C14, IPB C2 x IPB C4, IPB C2 x IPBC9, IPB C2 x IPB C10, IPB C14 x IPB C9, IPB C14 x IPB C10, IPB C14 x IPB C1, IPB C4 x IPB C9, IPB C4x IPB C10, IPB C4 x IPB C1, IPB C9 x IPB C10 dan IPB C10 x IPB C1.Kata kunci: Capsicum annuum, DGU, DGK, heterosisABSTRACTThe objectives of this research were to estimate general combining ability (GCA), specific combiningability (SCA) and heterosis of horticulture character. One set of population from half diallel mating, involving 6parents and 15 hybrids were used in this research. Randomized Completely Block Design was used in thisresearch with there replication. It was evidence that showed based on GCA, SCA heterosis and mean, 12 hybridi.e. IPB C2 x IPB C14, IPB C2 x IPB C4, IPB C2 x IPB C9, IPB C2 x IPB C10, IPB C14 x IPB C9, IPB C14 xIPB C10, IPB C14 x IPB C1, IPB C4 x IPB C9, IPB C4 x IPB C10, IPB C4 x IPB C1, IPB C9 x IPB C10 danIPB C10 x IPB C1 are potential to be made high yielding hybrid varieties.Key words: Capsicum annuum, GCA, SCA, heterosis
Co-Authors , Amisnaipa , Widodo Agus Riyanto Ahmad Satori Andi Takdir Makkulawu Bambang S. Purwoko D. Wasgito Purnomo Dini Rizkyah DWI SUBEKTI Endang Nurhayati Evalina C. Pandia Gede Suastika Irdika Mansur JAJAH KOSWARA Ketty Suketi Khaerin Nida Memen Surahman Muh. Taufik Muhamad Syukur Muhammad Syukur NOOR AIDAWATI PURNAMA HIDAYAT R Neny Iriany Rahmi Yunianti Roedhy Poerwanto RUSMILAH SUSENO Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Sientje Mandang Sumaraw Siti Hafsah Slamet Budijanto Sobir Sobir Sri Hendrastuti H SRI HENDRASTUTI HIDAYAT Sudirman Yahya Sulusi Prabawati U Undang Vitria Puspitasari Rahadi Vitria Puspitasari Rahadi Winarso D. Widodo Yundari, Yundari Zahratul Millah