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All Journal Program Kreativitas Mahasiswa - Penelitian Jurnal ORTOPEDAGOGIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Acta Biochimica Indonesiana
Andreas Budi Wijaya
Pendididikan dokter, Fakultas kedokteran, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

 1 
Characterization of TPO Enzyme and its Immunogenecity in New Zealand White Rabbits (Oryctolagus cuniculus) Ramadani, Devi; Wijaya, Andreas Budi; Srihardyastutie, Arie; Wuragil, Dyah Kinasih; Rosandi, Rulli; Rudijanto, Achmad; Aulanni'am, Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9539

Abstract

Hypothyroidism is characterized by inadequate production of thyroid hormones, chiefly thyroxine (T4) and triiodothyronine (T3). Thyroid peroxidase (TPO), is an enzyme and crucial for producing thyroid hormones, and also serves as a primary autoantigen in autoimmune thyroid conditions, such as Hashimoto's thyroiditis and Graves' disease. The existence of anti-thyroid peroxidase antibodies (anti-TPO) indicates an autoimmune response in which the immune system TPO which is normally recognized as part of the body (self), begins to be identified by the immune system as a something foreign (non-self). This study aimed to characterize, measure TPO activity, and evaluate the immunogenicity of TPO protein derived from patient serum and its ability to induce TPO antibody production in New Zealand White Rabbits. TPO protein isolation was achieved through SDS-PAGE and confirmed by western blot technique have molecular weight of 52 kDa. In samples from normal patients, the average TPO enzyme activity was 31.34 U/mL, while in hypothyroid patients, the average activity increased to 49.46 U/mL. TPO polyclonal antibody production in rabbits indicated its immunogenicity, peaking in week eight post-immunization, with an ELISA absorbance of 0.555. Dot blot analysis further confirmed anti-TPO sensitivity at various dilutions, anti-TPO has a high sensitivity at 1:80 dilution still detects TPO in sera patient.
Co-Authors Achmad Rudijanto Agung Kurniawan Arie Srihardyastutie Aulanni'am, Aulanni'am Aulanniam Camoya Gersom Devi, Athaayaa Nastiti Ulayya Editya Fukata Faizal Reza Pahlevi Furqan Hidayatullah Kiky Martha Ariesaka Prasetya, Ahmada Viosepta Putri, Angelica Igsanti Ramadani, Devi Rulli Rosandi Veronica Verina Setyabudhi Wuragil, Dyah Kinasih