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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal Farmasi Andalas Jurnal Sains dan Teknologi Farmasi Risâlah, Jurnal Pendidikan dan Studi Islam JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Farmasi Higea Destinesia : Jurnal Hospitaliti dan Pariwisata Journal of Education and Culture
Rustini Rustini
Universitas Kuningan
Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Other

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Kajian Biodegradasi Filem Plastik Campuran Polistiren dengan Poli(3hidroksibutirat-ko-3-hidroksivalerat) dalam Tanah Secara In-vitro Permatadewi, Asiska; Zaini, Erizal; Rustini, Rustini; Djamaan, Akmal
Jurnal Farmasi Andalas Vol 1, No 1 (2013)
Publisher : Jurnal Farmasi Andalas

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Abstract

The in vitro biodegradation testing of plastic of the mixture of polystyrene (PS) and poly (3-hydroxybutirate-co-3-hydroxyvalerat) [P(3HB-ko-3HV)] in soil has been carried out. Plastic film was produced by using blending technique and followed by solved casting at various composition. The biodegradation testing was conduted by using soil burial method. Rseult showed that the increasing of component [P(3HB-ko-3HV)] in the mixture of plastic film was also increase the biodegradation rate of plastic film in the soil. At the composition 100:0, 95:5, 90:10 and 85:15 of plastic film in 5 weeks burial was found weigh remaining of plastic were of 99.67; 95.71; 95.03; 94.13% w/w respectively. It is also observed that the amount of bacteria in the soil were of 2.57 x 106 colonies/g.
Uji Biodegradasi Filem Plastik Campuran Polistiren dengan Poli(3 Hidroksibutirat-Ko-3-Hidroksivalerat) dalam Lumpur Dewi, Asiska Permata; Octaviani, Melzi; Rustini, Rustini; Zaini, Erizal; Djamaan, Akmal
Jurnal Sains dan Teknologi Farmasi Vol 17 No 1 (2012)
Publisher : Fakultas Farmasi Universitas Andalas

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Abstract

An assay of biodegradation of plastic film combination of polystyrene (PS) and poli (3-hydroxy butyrate-ko-3-hydroxyvalerate) [P(3HB-ko-3HV)] has been conducted. The assay was done by using soil burial test method. The plastic film was made by blending technique of various composition of PS/P(3HB-ko-3HV) in to the mud. The remaining weight of plastic film was determined gravimentically time intervals 1,2,3,4 and 5 weeks of burial. Result showed that the P(3HB-ko-3HV) had an influence on the degradation rate of plastic film. The biodegradation rate increased with an increase in P(3HB-ko-3HV) content in the mixture. The rate was derived from linear regression equation and parameters such as biodegradation rate constant (k), t 50 % and t 95% were obtained. It was found that for combination of  PS/P(3HB-ko-3HV) 95:5 k= 1,26% b/b per week , t 50 % = 39,07 weeks and t 95 % = 74,783 week for the mixture of PS/P(3HB-ko-3HV) 90:10  k = 1,604 % b/b per week, t 50 % = 30,57 week and t 95 % = 58,62 week. For the mixture of PS/P(3HB-ko-3HV), 85:15 k = 2,663 % b/b per week, t 50 % = 17,95 week and t 95 % = 34,85 week. While those of pure PS plastic film k = 0,24 % b/b per week, t 50 % = 201,85 week and t 95 % = 354,55 week which indicated a very slow biodegradation. Analysis by SEM showed that the surface of plastic film was eroded and damaged during the burial. The amount of bacterial colony per 1 gram of mud was 2,31x107.
PENINGKATAN PRESTASI BELAJAR SEJARAH DENGAN STRATEGI PEMBELAJARAN INSIDE-OUTSIDE CIRCLE Rustini, Rustini
Risâlah, Jurnal Pendidikan dan Studi Islam Vol 8 No 1 (2022): Pendidikan dan Studi Islam
Publisher : Fakultas Agama Islam Universitas Wiralodra Indramayu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/jurnal_risalah.v8i1.238

Abstract

Penelitian dilakukan dalam bentuk penelitian tindakan kelas dengan dua siklus, mengetahui bagaimana prestasi belajar peserta didik dengan Strategi pembelajaran Inside-Outside Circle (IOC), apakah dengan strategi pembelajaran Inside-Outside Circle (IOC) dapat meningkatkan hasil belajar peserta didik dengan kompetensi dasar. Hasil penelitian siklus I hasil Hasil Belajar Siklus I pada aspek pengetahuan baru mencapai 28 peserta didik (77,78%), siklus II meningkat mencapai 32 peserta didik (88,89%), aspek ketrampilan pada Siklus I 24 peserta didik (66,67%) pada Siklus II mencapai 32 peserta didik (88,89%), pada aspek sikap tuntas lima karakter bangsa 30 peserta didik (83,33%), pada siklus II aspek sikap tuntas lima karakter bangsa 33 peserta didik (91,67%). Simpulan hasil penelitian ini adalah: 1)proses pembelajaran menggunakan strategi pembelajaran Inside – Outside Circle (IOC) dapat dapat diketahui prestasi belajarnya 2) terjadi peningkatan kompetensi dasar; dan 3) terbukti ada kebenaran antara teori yang disampaikan oleh Ahli Ilmu pendidikan dan Permendikbud dengan proses pembelajaran dalam upaya peningkatan Hasil Belajar.
Formulasi dan Uji Aktivitas Gel Hand Sanitizer Ekstrak Etanol Daging Buah Pala (Myristica Fragrans Houtt) dalam Menghambat Pertumbuhan Staphylococcus Aureus Usman, Hendrizal; Rustini, Rustini; Eka Putri, Lusia; Tri Andrica, Popy; Dwinatrana, Khiky
Journal of Education and Culture Vol. 4 No. 2 (2024): Journal of Education and Culture
Publisher : Indra institute Research & Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58707/jec.v4i2.853

Abstract

Membersihkan tangan adalah cara penting untuk menjaga kesehatan dan mencegah penyakit. Pembersih tangan berbasis alkohol semakin populer karena kemudahan akses dan efektivitasnya dalam mengurangi kuman, terutama di rumah sakit. Staphylococcus aureus, penyebab infeksi yang berasal dari komunitas dan nosokomial, menjadi tantangan klinis karena peningkatan resistensi antimikroba. Buah pala (Myristica fragrans Houtt) memiliki kandungan metabolit sekunder seperti sabinene, myristicin, elemicin, asam tetradekanoat, phellandrene, dan myrcene yang memiliki aktivitas antibakteri dengan spektrum luas. Penelitian ini bertujuan untuk membuat gel pembersih tangan yang mengandung ekstrak pala, efektif melawan S. aureus. Hasil penelitian ini menunjukkan bahwa sediaan Gel hand sanitizer ekstrak etanol daging buah pala menujukkan aktifitas sebagai antibakteri terhadap S.aureus dengan nilai rata-rata diameter zona hambat pada F1 sebesar (16.06±0.09) dan F2 sebesar (20.53±0.12). Gel hand sanitizer ekstrak daging buah Pala F1 dan F2, memiliki aktifitas antibakteri dengan kategori kuat dan sangat kuat terhadap bakteri S. aureus. Penggunaan bahan alami dalam hand sanitizer diketahui lebih aman, memiliki efek samping minimal, dan ramah lingkungan. Formulasi ini menawarkan solusi efektif dan berkelanjutan untuk kebersihan tangan.
Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties Rustini, Rustini; Rahmi, Khalila Rahmi; Putra, Purnawan Pontana; Andayani, Regina Andayani; Dwinatrana, Khiky
Pharmaciana Vol. 14 No. 3 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i3.28432

Abstract

Avocado peels (AVP) are renowned for their potent antioxidant properties, making them highly effective in preventing oxidation and free radical formation. Endophytes, microorganisms residing within plant tissues, have demonstrated the ability to produce novel compounds with remarkable biological activities. These bioactive compounds are sometimes even more potent than those found in their host plants. This study explores the potential of endophytes from avocado peels as rich sources of antioxidant compounds. AVP samples are collected, surface-sterilized, and segmented before being cultured in growth media. The bacteria are then isolated, purified, and subjected to ethyl acetate extraction to evaluate their antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) microassay. Samples demonstrating favorable antioxidant properties undergo molecular identification through 16S rRNA gene sequencing. Four bacterial strains are successfully isolated, with only the APK4 strain exhibiting significant antioxidant activity with an IC50 value of 302.3 μg/mL. Molecular analysis and phylogenetic tree construction reveal that APK4 is closely related to the Enterobacter hormaechei species, with a percent identity value of 99.93%. These findings highlight the potential of active metabolites from endophytic bacteria in AVP extracts as promising lead compounds for the development of novel drugs, nutraceuticals, and cosmetic ingredients.
Uji Aktivitas Antibakteri Ekstrak Bakteri Endofit dan Identifikasi Bakteri yang Diisolasi dari Kulit Jeruk Nipis (Citrus aurantifolia Swingle) Rustini, Rustini; Ismed, Friardi; Nabila, Gina Sasha
JSFK (Jurnal Sains Farmasi & Klinis) Vol 9 No 1 (2022): J Sains Farm Klin 9(1), April 2022
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.9.1.42-49.2022

Abstract

Kulit Jeruk nipis (Citrus aurantifolia Swingle) merupakan bagian tanaman yang banyak digunakan untuk pengobatan dengan berbagai aktivitas farmakologis. Namun masih sedikit diketahui kajian tentang bakteri endofit yang terkait dengan kulit buah tanaman ini. Penelitian ini bertujuan untuk mengisolasi bakteri endofit dari kulit buah tanaman ini serta menguji aktivitas dalam mengambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Methicilin-Resistant Staphylococcus aureus (MRSA). Sebanyak enam Isolat bakteri berhasil diisolasi lalu difermentasi dalam media Nutrient Broth (NB).  Hasil fermentasi kemudian diekstraksi menggunakan pelarut etil asetat. Ekstrak kental masing-masing isolat diujikan aktivitas antibakterinya menggunakan metode difusi Kirby-Bauer. Berdasarkan hasil pengukuran zona hambat menunjukkan bahwa ekstrak dari isolat 3 dan isolat 5 memiliki diameter hambat kuat >10 mm. Hasil identifikasi fitokimia secara kualitatif menunjukkan bahwa ekstrak dari isolat 3 menujukkan adanya kandungan alkaloid dan flavonoid sedangkan isolat 5 teridentifikasi senyawa alkaloid, flavonoid dan polifenol. Hasil identifikasi penamaan bakteri endofit menunjukkan bahwa isolat 3 merupakan Bacillus velezensis strain JS25R dan isolat 5 merupakan Staphylococcus sp. Ekstrak dari bakteri endofit ini terbukti memiliki aktivitas terhadap bakteri patogen dan potensial untuk dilakukan isolasi dan pengembangan terhadap senyawa metabolit sekunder yang memiliki aktivitas tersebut. 
AKTIVITAS ANTIMIKROBA FRAKSI N-HEKSAN DAN FRAKSI METANOL JAMUR ENDOFIT (Trichoderma harzianum NT3) TERHADAP PERTUMBUHAN MIKROBA Staphylococcus aureus, Escherichia coli, MRSA, DAN Candida albicans SECARA IN VITRO Usman, Wilda Fhitriany; Handayani, Dian; Rustini, Rustini
Jurnal Farmasi Higea Vol 17, No 1 (2025)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52689/higea.v17i1.665

Abstract

Penelitian ini merupakan penelitian lanjutan dalam rangka menemukan aktivitas antimikroba dari fraksi jamur endofit Trichoderma harzianum. Isolat murni jamur endofit Trichoderma harzianum NT3, dikultivasi menggunakan media beras padat selama 3-4 minggu pada suhu ruang. Kemudian dilakukan ekstraksi menggunakan pelarut etil asetat (EtOAc). Ekstrak yang diperoleh kemudian dipekatkan menggunakan Rotary Evaporation. Kemudian dilakukan fraksinasi dengan metode fraksinasi cair cair menggunakan pelarut metanol dan n-heksan. Aktivitas antimikroba fraksi diuji terhadap S. aureus, E. coli, MRSA, dan Candida albicans menggunakan metode difusi agar. Pada penelitian ini disimpulkan bahwa fraksi n-heksan merupakan fraksi yang paling efektif melawan S. aureus, E. coli, MRSA, dan Candida albicans dengan diameter hambat masing-masing yaitu 11,50 mm, 12,35 mm, 11,20 mm, dan 13,50 mm. Namun masih diperlukan penelitian lebih lanjut untuk menemukan senyawa yang memiliki antivitas antimikroba tersebut.
Analisis Faktor Pendorong Dan Faktor Penarik Yang Memengaruhi Minat Kunjung Pengunjung (Studi Kasus Event Pekan Raya Jakarta 2022 Di Jiexpo Kemayoran) Nur, Widya Ningsih; Rustini, Rustini
Destinesia : Jurnal Hospitaliti dan Pariwisata Vol. 6 No. 1: September 2024
Publisher : Institut Ilmu Sosial dan Manajemen STIAMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31334/jd.v6i1.4701

Abstract

Push Factors dan Pull Factors merupakan dua faktor yang dapat mempengaruhi pengunjung ingin berkunjung ke Jakarta Fair 2022, pengunjung ingin berkunjung ke PRJ yaitu untuk mencari promo, menikmati hiburan, konser musik dan mencari kuliner. Tujuan dari penelitian ini adalah untuk menganalisis seberapa besar pengaruh Push Factors dan Pull Factors terhadap Minat Pengunjung pada Event Jakarta Fair 2022 Secara Parsial dan Simultan. Metode penelitian ini adalah Kuantitatif, dengan menggunakan variabel Push Factor dan Pull Factor sebagai Variabel Independen, Variabel Minat Berkunjung sebagai Variabel Dependen. Jumlah populasi pada tahun 2022 sebanyak 6,9 juta jiwa dengan menggunakan 100 sampel. Hasil penelitian menunjukkan bahwa variabel faktor pendorong yang secara parsial mempengaruhi minat pengunjung sebesar 0,640 atau 64%, berdasarkan hasil uji T diperoleh nilai t hitung sebesar 13,206 > nilai t tabel sebesar 1,984 maka H0 ditolak dan H1 diterima. Pada Variabel Faktor Penarik yang secara parsial berpengaruh terhadap minat berkunjung sebesar 0,549 atau 54,9% berdasarkan hasil uji T bahwa nilai t hitung sebesar 10,912 > nilai t tabel sebesar 1,984 maka H0 ditolak dan H2 diterima. Sedangkan Variabel Faktor Pendorong dan Faktor Penarik yang berpengaruh terhadap Minat Berkunjung secara simultan sebesar 0,712 atau 71,2%. Hal ini menunjukkan bahwa pengaruh Faktor Pendorong dan Faktor Penarik secara simultan terhadap Minat Berkunjung sebesar 71,2% berdasarkan hasil uji F bahwa nilai f hitung sebesar 119,876 > nilai f tabel sebesar 3,09 maka hal ini membuktikan bahwa H0 ditolak dan H3 diterima.
Pengaruh Daya Tanggap Staf Hotel Terhadap Kepuasan Tamu Karantina di Hotel Best Western Plus Kemayoran Putri, Inchi Yurana; Rustini, Rustini
Destinesia : Jurnal Hospitaliti dan Pariwisata Vol. 5 No. 1: September 2023
Publisher : Institut Ilmu Sosial dan Manajemen STIAMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31334/jd.v5i1.3412

Abstract

This study is to aimed to identify the influence of hotel staff responsiveness on quarantine guest’ satisfaction at Best Western Plus Kemayoran Hotel. The background of this study is the increasing outbreak of the corona virus which has caused every citizen who is in another country unable to return to their country of origin before carrying out a quarantine that has been regulated by the government. This analysis was conducted by the independent variable, namely the responsiveness of the hotel staff and the dependent variable which is the guests’ satisfaction. The data used by the researchers were taken from the total population of 12,048 quarantine guests in 2021 which the researchers then counted using the Slovin formula, hence the sample used in this study was 100 guest respondents who carried out quarantine at Best Western Plus Kemayojran Hotel by distributing questionnaires using the simple random sampling method which were then processed using a validity test, reliability test, hypothesis test, variable descriptive analysis, normality test, R2 determinant test, and T test. The value of the coefficient of determination (R2) on the symbol R square with a value of 0.592 or 59,2%, the value of t count 11.917 > table 1.984 with these results it can be concluded that H0 is rejected and H1 is accepted and it indicates that the variable Hotel Staff Responsiveness (X) significantly influence Guests’ Satisfaction (Y).
Pengaruh Harga Dan Bukti Fisik Terhadap Keputusan Menginap di Rail Transit Suite Gambir Kusumadewi, Isnaeni; Rustini, Rustini
Destinesia : Jurnal Hospitaliti dan Pariwisata Vol. 5 No. 2: Maret 2024
Publisher : Institut Ilmu Sosial dan Manajemen STIAMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31334/jd.v5i2.3813

Abstract

This research aimed to find out how much influence of price and physical evidence toward the decision to stay at the Gambir Rail Transit Suite. This research was motivated by some negative ulasans on the online travel agent Rail Transait Suite Gambir where guests who stayed there stated that the price of the stay was not commensurate with what they received and also complained about the physical evidence available. The method of this research was a quantitative method with data collection technique using questionnaires and observation. The population in this research was guests staying at the Rail Transit Suite Gambir. The number of samples successfully processed using the Slovin formula was 100 people who stayed at the Gambir Rail Transit Suite. The data analysis technique in this research used the validity test, classical assumption test, and multiple linear regression analysis with the t test and F test. Then the data was processed using the IBM SPSS 26 software program. The results of the coefficient of determination (R2) of this research showed the independent variable influenced the decision to stay partially (attached variable), namely price had a positive influence of 73.1% with t hitung value of 6.522 > t table 1.988 with significance value of 0.000 < 0.05 and physical evidence had a positive influence of 73.2% with t hitung value 6.553 > t table 1.988 with significance value of 0.000 < 0.05. Simultaneously, price and physical evidence had a positive influence on the decision to stay with F t hitung value of 211.382 > F table 3.09 with significance value of 0.000 < 0.05 and the coefficient of determination (R2) for price and physical evidence was 81%, while the remaining 19% was influenced by other variables not included in this research.
Co-Authors AA Sudharmawan, AA Akmal Djamaan Andayani, Regina Andayani Asiska Permata Dewi Asiska Permatadewi Dian Handayani Dwinatrana, Khiky Eka Putri, Lusia Erizal Zaini Friardi Ismed Kusumadewi, Isnaeni Melzi Octaviani Nabila, Gina Sasha Nur, Widya Ningsih Putri, Inchi Yurana Rahmi, Khalila Rahmi Tri Andrica, Popy Usman, Hendrizal Usman, Wilda Fhitriany