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Phytochemical Screening, Determination of Total Phenolic Contents and Total Flavonoid Contents of Pod Purified Extract from Caesalpinia pulcherrima (L.) Sw. Sovia Lenny; Febriyanti Br Surbakti; Juliati Br Tarigan
Journal of Chemical Natural Resources Vol. 7 No. 1 (2025): Journal of Chemical Natural Resources (JCNaR)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v7i1.19838

Abstract

Caesalpinia pulcherrima (L.) Sw. is traditionally used in medicine and is thought to be influenced by the presence of secondary metabolites, especially phenolic compounds and flavonoids. However, identifying the phytochemical compounds of Caesalpinia pulcherrima (L.) Sw. plants, especially on the pods, are less discussed. Here, we propose research on the identification of secondary metabolite compounds of pod-purified extract from Caesalpinia pulcherrima (L.) Sw. using phytochemical screening and also the determination of total phenolic and total flavonoid content. The extraction of Caesalpinia pulcherrima (L.) Sw. pods were processed by maceration and fractionation methods to obtain the purified extract. Total phenolic contents were determined using the Folin-Ciocalteu method. Meanwhile, total flavonoid contents were determined using the AlCl3 method. The result showed that the purified extract of Caesalpinia pulcherrima (L.) Sw. pods contained phenolics, flavonoids, alkaloids, and saponins. The total phenolic and total flavonoid content in the pod purified extract were 326.038 mg GAE/g extract and 290.026 mg QE/g extract, respectively.
Screening of Phytochemicals and Determination of Total Phenolic and Flavonoid Contents in Different Plant Parts of Kalanchoe ceratophylla Haw. Sovia Lenny; Alyssa Zahra Br Karo; Lamhot Parulian Manalu; Astuti
Journal of Chemical Natural Resources Vol. 7 No. 2 (2025): Journal of Chemical Natural Resources (JCNaR)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v7i2.22964

Abstract

Kalanchoe ceratophylla Haw. is a medicinal plant traditionally used to address various health disorders. Its biological activities are believed to be associated with its secondary metabolites, particularly phenolics and flavonoids. Despite its traditional use, scientific information This study assesses the phytochemical composition alongside the total phenolic and flavonoid contents in the stem and leaf preparations of Kalanchoe ceratophylla Haw. Phytochemical screening confirmed the presence of alkaloids, flavonoids, tannins, and phenols in the stem extract, while the leaf extract also contained steroids. The total phenolic content was 7.43 mg GAE/100 g extract for stem and 7.11 mg GAE/100 g extract for leaves, whereas total flavonoids amounted to 0.324 mg QE/g extract (stem) and 1.621 mg QE/g extract (leaves). These findings highlight the potential use of K. ceratophylla, especially its leaves, for developing antioxidant-based herbal medicines.
Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense YURNALIZA YURNALIZA; DITA ISNAINI RAMBE; LISTRA SARIMUNGGU; MEYLISA PURBA; ISNAINI NURWAHYUNI; SOVIA LENNY; ANISA LUTFIA; ADRIAN HARTANTO
Biodiversitas Journal of Biological Diversity Vol. 21 No. 8 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210803

Abstract

Abstract. Yurnaliza. Rambe DI, Sarimunggu L, Purba M, Nurwahyuni I, Lenny S, Lutfia A, Hartanto A. 2020. Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense. Biodiversitas 21: 3431-3437.  Burkholderia spp. are ubiquitous diazotrophic bacteria within ?-Proteobacteria group, known for its occurrence in variety of niches from aquatic to terrestrial habitats and as endophytes. Beneficial strains of Burkholderia have been reported as plant growth-promoting rhizobacteria or as antagonistic bacteria against phytopathogenic fungi. This study evaluated the potential of multiple strains of Burkholderia spp. recovered from three ecological niches, such as rhizosphere, humus soil, and root endophytes of oil palm plantations in North Sumatra to suppress the growth of basal stem rot causative agent in oil palm (Elaeis guineensis Jacq.) by Ganoderma boninense Pat. The antagonistic isolates were identified on the basis of molecular identification using 16S rDNA sequence (27F–1462R), revealing twelve isolates (48%) as members of Burkholderia cepacia complex (Bcc), with other minor genera, such as Chitinophaga, Klebsiella, Mycobacterium, Paenibacillus, Rhizobium, Serratia, Stenotrophomonas, and Xanthomonas. The antagonistic activities as expressed in the percentage inhibition of radial growth (PIRG) against G. boninense were considerably potential with the highest percentage of 55%. In comparison, the crude extract (MeOH, EtOAc) was also tested against G. boninense colonies showing PIRG from 0 to 38%. Majority of isolates did not show any visible chitinolytic activity based on plate assay, in exception to B. contaminans RC02 while most of them were producers of glucanase. The collection of indigenous Burkholderia spp. originating from North Sumatran oil palm plantations, i.e. B. cepacia, B. contaminans, B. metallica, and B. stagnalis may then be considered as potential biocontrol agents against G. boninense based on their antagonistic activities, antifungal properties, and hydrolytic enzyme activities.
Study of Bendo Sap (Artocarpus Elasticus Reinw) Polyurethane Adhesive on Modified Asphalt through Marshall Testing Adiansyah Adiansyah; Tamrin Tamrin; Marpongahtun Marpongahtun; Sovia Lenny; Lua Shun Kuang; Ronn Goei
Journal of Multidisciplinary Applied Natural Science Vol. 6 No. 3 (2026): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.435

Abstract

Bendo latex–based polyurethane (BD–PU) adhesive demonstrates strong potential as a bio-based modifier for improving asphalt performance and providing a sustainable alternative to petroleum-derived polymers. The novelty of this study lies in the utilization of bendo latex as a locally sourced renewable feedstock for polyurethane synthesis and its direct application as an asphalt modifier, which remains largely unexplored compared to conventional petroleum-based or partially bio-based polyurethane systems. In this work, bendo latex was converted into polyol via controlled condensation and addition reactions, followed by blending with polyethylene glycol (PEG 1000) and reaction with toluene diisocyanate (TDI) to produce BD–PU adhesive. The synthesized polyurethane exhibited mechanical properties comparable to conventional isocyanate-based polymers, confirming its suitability for asphalt modification. At the optimum BD–PU content of 20 wt%, the modified asphalt demonstrated a Marshall stability of 27.6 kN, a flow value of 4.5 mm, and a stiffness of 6.5 kN/mm. It represents a significant improvement over the control asphalt, with a 75% increase in Marshall stability and an 85% enhancement in stiffness, indicating markedly improved resistance to permanent deformation. These improvements are attributed to the formation of a crosslinked polyurethane network that enhances interfacial bonding between asphalt and aggregates, resulting in improved load distribution and structural integrity. Compared to previously reported polyurethane-modified asphalt systems, this study offers a key contribution by (i) introducing a fully bio-based polyol derived from bendo latex, (ii) demonstrating competitive or superior mechanical performance, and (iii) promoting the use of locally available renewable resources for sustainable infrastructure materials. Overall, the findings highlight the significant potential of BD–PU as an eco-friendly and high-performance asphalt modifier, supporting the development of more durable and sustainable road construction materials.