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Penggunaan Gen GH sebagai Marka Molekuler DNA Gurami, Osphronemus goramy dalam Pengembangan Teknologi Surrogate Broodstock Achmad, Marlina; Alimuddin, Alimuddin; Carman, Odang; Arfah, Harton; Zairin, Muhammad
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.7

Abstract

The technology of fish germ cell transplantation had been established to create broodstock systems by which a target offspring can be produced from a surrogate parent. Donor cell for transplantation is derived from transgenic fish carrying green fluorescent protein gene functions as a marker to distinguish the donor from recipient cell. In this study, an alternative technique was developed for identifying gouramy-derived donor cell and Nile tilapia as recipient by PCR amplification method using growth hormone (GH) gene as a molecular marker.  Specific primer for GH gouramy was designed by using Genetyx version 7 software.  ß-actin gene was used as an internal control of DNA loading. The result showed that a specific PCR amplification product of 340 in length was obtained when the DNA template was from gouramy, while no PCR product from Nile tilapia. The minimum concentration of genomic DNA of gouramy mixed with a 700 ng/µl of Nile tilapia that could be detected by PCR was 1 ng/µl. Thus, PCR method with specific GH primer may be useful to detect the incorporation of donor cell in recipient gonad towards development of surrogate broodstock technology.
Pengelolaan Sampah Untuk Produksi Magot Sebagai Bahan Baku Alternatif Untuk Ikan Air Tawar Azis, Hasni Yulianti; Sukardi, Reski Wahyuni; Achmad, Marlina; Cangara, Arie Syahruni; Asnur, Andi Nurfadilah; Wahab, Fachrul; Saputra, Anugerah
MESTAKA: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 6 (2024): Desember 2024
Publisher : Pakis Journal Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58184/mestaka.v3i6.548

Abstract

Maggot (Black Soldier Fly/BSF larvae) is a high-protein resource that can be utilized as an alternative raw material for fish feed. Maggot production has great potential to reduce dependence on commercial feed while also utilizing organic waste. This community service program aims to introduce maggot production technology to fish farmers in Soppeng Regency, South Sulawesi, by using household organic waste as feed for snakehead fish (Channa striata). The activities include counseling on organic waste sorting, maggot cultivation training, and the application of maggot as fish feed. Through this program, the community gained an understanding of how to manage waste into high-quality maggot and use it as fish feed. The results showed that the community not only understood the maggot production process but also successfully utilized it independently to reduce fish feed costs. This program successfully raised awareness about the importance of organic waste management, supported freshwater fish farming, and had a positive impact on the environment. By utilizing maggot as an alternative feed, it is hoped that the community can develop more efficient and environmentally friendly fish farming, while also creating a sustainable organic waste management model.
Sosialisasi Penerapan Minyak Buah Merah Pada Pakan Formulasi Bagi Usaha Budidaya Udang Vanname PT. Kreatif Laut Indonesia, Kabupaten Barru Achmad, Marlina; Zainuddin, Zainuddin; Novita Sari, Dian; Fadhlullah M, Muhammad; Syahruni Cangara, Arie; Saputra, Anugerah; Akram, Muhammad; Suteji Anwar, Andika; Fathul, Muh.; farendi, Arya; Nur Amsal, A.Muh Ihsan
JDISTIRA - Jurnal Pengabdian Inovasi dan Teknologi Kepada Masyarakat Vol. 4 No. 2 (2024)
Publisher : Yayasan Rahmatan Fidunya Wal Akhirah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58794/jdt.v4i2.1106

Abstract

Budidaya udang vaname memiliki potensi ekonomi besar, dan salah satu aspek yang mempengaruhi kesehatan larva udang vaname adalah kandungan nutrisi dalam pakan. Minyak buah merah dikenal dalam pengobatan tradisional sebagai sumber nutrisi yang kaya akan senyawa bioaktif asam lemak tak jenuh dan fitosterol, zat-zat tersebut mudah dicerna dan diserap sehingga memperlancar proses metabolisme. Program sosialisasi penerapan minyak buah merah pada pakan udang dilaksanakan di lokasi budidaya udang vanname PT. Kreatif Laut Indonesia (PT.KLI). Kegiatan ini bertujuan untuk meningkatkan pemahaman serta skil menekstrak minyak buah merah serta membuat pakan formulasi berbasis fitooil bagi tim pembudidaya PT. KLI. Metode yang digunakan pada program ini adalah pelatihan yang meliputi demonstrasi langsung pembuatan ekstrak minyak buah merah, dan pembuatan pakan formulasi. Selanjutnya, dilakukan pre-test dan post test untuk menilai pemahaman peserta terhadap kegiatan yang dilakukan. Hasilnya, pemahaman peserta meningkat seiring dengan pendampingan program yang dilakukan, dan peserta dapat membuat pakan formulasi udang secara mandiri.
Identifikasi Ektoparasit Pada Ikan Koi (Cyprinus carpio) Di Makassar Bunga, Margaretha; Achmad, Marlina
Torani Journal of Fisheries and Marine Science VOLUME 1 NOMOR 1, DESEMBER 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v1i1.3794

Abstract

 Penelitian bertujuan untuk mengidentifikasi ektoparasit pada ikan mas koi (Cyrinus carpio) di Makassar. Penelitiandilakukan dengan pengambilan sampel sebanyak tiga kali di bulan April hingga Mei 2017. Sampel ikandimasukkan ke dalam kantong plastik yang telah diisi dengan oksigen. Jumlah sampel adalah 45 ikan denganpanjang 5,5-11,8 cm dan berat 5,12 – 13,2 g. Identifikasi parasit dan penghitungan jumlah parasit dilakukan diLaboratorium Parasit dan Penyakit Ikan, Departemen Perikanan, Fakultas Ilmu Kelautan dan PerikananUniversitas Hasanuddin. Data dianlaisis secara deskriptif. Serangan ektoparasit menyerang ikan koi di bagianinsang yakni 34,21%, sirip dorsal 34,21%, dan pada mukus mengandung 31,58%.Kata kunci : ektoparasit, identifikasi, ikan koi
Karakteristik Jaringan Secara Histologi dari Strain Rumput Laut (Kappaphycus alvarezii) yang Terinfeksi Penyakit Ice-Ice Maulani, Resi Kumala; Achmad, Marlina; Latama, Gunarto
Torani Journal of Fisheries and Marine Science VOLUME 1 NOMOR 1, DESEMBER 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v1i1.3796

Abstract

 Salah satu yang menyebabkan kegagalan budidaya rumput laut Kappaphycus alvarezii  yaitu terserangnya penyakitice – ice . Penyakit ice-ice merupakan penyakit yang umum terjadi pada rumput laut kappaphycus  dengan gejalaterjadinya pemutihan pada bagian thallus  dan penyakit ini dapat menyebabkan kematian massal pada produksirumput laut kappaphycus alvarezii . Salah satu cara yang dapat dilakukan untuk menganalisa bagian thallus  rumputlaut Kappaphycus alvarezii  yaitu uji histopatologi. Penelitian ini bertujuan untuk mengetahui perubahan bentuksel pada kondisi jaringan thallus  rumput laut K.alvarezii  yang terserang penyakit ice- ice  menggunakan metodehistopatologi. Pengambilan sampel rumput laut yang digunakan jenis Kappaphycus alvarezii  varietas coklat danhijau dengan 1 titik masing – masing varietas. Sampel rumput laut terdiri atas 2 sampel yaitu bagian thallus  yangterserang penyakit ice-ice  dan bagian thallus  tanpa gejala ice-ice . Sampel berumur 15 hari. Sampel yang dipilihuntuk pemeriksaan, dipotong setebal 0,5 - 1 cm. Sampel dimasukkan kedalam botol sampel untuk difiksasi ± 1minggu, yang berisi formalin 10%.Tahapan pembuatan preparat histopatologi terdiri atas fiksaksi, dehidrasi,clearing , impregnasi , embedding , cutting , staining , dan pemeriksaan dibawah mikroskop. Penelitian ini dianalisissecara dekskriptif kualitatif dengan melakukan pengamatan gambar histopatologi pada jaringan rumput laut K.alvarezii . Jaringan thallus  yang yang terserang penyakit ice-ice  mengalami perubahan bentuk sel, bagian lapisanluar terlihat tidak utuh, hancur, sehingga mengalami kematian sel dan bagian korteks dalam dinding sel terlihattidak teratur dan renggang, tidak terbentuk bulat atau lonjong, sebagian sel mengecil. Jaringan thallus  yang tidaknormal mengalami gejala Hipoplastik (terhambatnya atau terhentinya pertumbuhan sel) dan Nekrotik (kematiansel).Kata kunci : Kappaphycus alvarezii, penyakit ice – ice, Histopatologi, Hipoplastik, Nekrotik
Use of Probiotics to Fight Bacterial Populations of Vibrio sp. on Vaname Shrimp Cultivation (Litopenaeus vannamei) Mustafa, Muhammad Fadhil; Bunga, Margaretha; Achmad, Marlina
Torani Journal of Fisheries and Marine Science VOLUME 2 NOMOR 2, JUNI 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v2i2.7056

Abstract

The consequence of super intensive shrimp farming is an increase in aquaculture waste and disease transmission. Vibrio sp. Bacteria is a disease-causing agent in shrimp larvae when the shrimp is in a state of stress and weakness, and can cause death. The application of probiotics, namely beneficial microorganisms can degrade organic matter, reduce disease, and help accelerate the process of nutrient cycles as a consequence of super intensive shrimp farming. Commercial probiotics currently available are a mixture of several types of beneficial microorganisms, namely Bacillus sp., Pseudomonas sp., Nitrosomonas sp., Aerobacter sp., and Nitrobacter sp. The purpose of this study was to evaluate the use of probiotics with different time intervals to produce a decrease in the population of Vibrio sp. The research method applied was the treatment of commercial probiotics at different intervals of three, five, and seven days and without the administration of probiotics as controls. Furthermore, the bacterial population was calculated by total plate count (TPC). The results showed that the administration of probiotics had a significant effect on the decrease in the population of Vibrio sp. Provision of probiotics three days apart showed a decrease in the number of bacterial populations that were significantly different from the controls, but the same as the administration of intervals of five and seven days. The decrease in the number of bacterial populations in the treatment interval of three, five, and seven days in a row is 0.05 x , 0.41 x , and 0.61 x  CFU / ml. This study recommends that the use of commercial probiotics intervals of seven days for super intensive vaname shrimp farming. The use of probiotics for three days indicates an impact on the increase in expenditure costs for probiotics. Commercial probiotic studies modified with natural microorganisms are thought to be able to streamline the operational costs of shrimp farming.Keywords: Vibrio sp. bacteria, probiotics, vaname shrimp, super intensive cultivation
Effect of Basin Color on The Performance of Blue Swimming Crab (Portunus pelagicus) Muthmainnah, Muthmainnah; Karim, Muhammad Yusri; Achmad, Marlina
Torani Journal of Fisheries and Marine Science VOLUME 4 NOMOR 1, DESEMBER 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35911/torani.v4i1.12878

Abstract

Blue swimming crab (Portunus pelagicus) is a sea crab that is widely found in Indonesian waters. One of the determinants of the success of crab cultivation is the availability of quality seeds. Howewer, blue swimming crab hatchery is currently experiencing problems, namely unstable seed availiability due to high mortality and growth at the larvae stage. This research aims to evaluate the effect of various colors of rearing basins of feeding rate and survival rate of blue swimming crab larvae (P. pelagicus) in zoea stage. The larvae for the study was zoea-1 stage with a stocking density of 50 ind/L. The basins used in this study was a round plastic basin with a volume of 40 L filled with 30 L of media water. The feed used was rotifer and artemia nauplius. The method used was a completely randomized design consist of 4 treatments and 3 replications. The treatments that were the use black, green, blue, and red basins colors. The results of the analysis of variance showed that the color of the basins had a significant effect on the feeding rate (p < 0,05) and very significant on survival rate (p < 0,01) for small blue swimming crab larvae were produced in black basins 90.14 and 16.60%.
The Effect of Adding Egg Shells to Moist Feed on The Specific Growth and Survival Rate of Lobster Panulirus sp Achmad, Marlina; Widarma, I Gede Shindu; Fadilah, Mifta Nur; Ramadhan, Riski; Putri, Siti Arleneyanti
Torani Journal of Fisheries and Marine Science VOLUME 5 NOMOR 1, DESEMBER 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35911/torani.v5i1.18738

Abstract

Sea lobster is a commodity that has high value in both national and international markets. Globally, the demand for sea lobster is increasing by about 15% per year. During growth, lobsters undergo a moulting process. Moulting/ecdysis is a very important process in the life cycle of arthropods (including decapods) because shell release is a must for growth and metamorphosis. In improving and assisting the molting process in lobsters, it is assisted by the provision of additional calcium from egg shells. Egg shells contain 94% calcium carbonate, 1% calcium phosphate, 1% magnesium carbonate and 4% organic compounds. In this study, an experiment was conducted on the addition of feed with a mixture of egg shells to support post-moulting optimization and supplement calcium requirements for lobster larvae. The percentage of egg shells as treatment, namely treatment A (0%), B (5%), C (10%), and D (15%) of the total feed to be made for sea lobster. Maintenance is carried out in a controlled tub, for four months. The highest specific growth obtained in this treatment was treatment A (0.06-0.21%) and the lowest was treatment D (-0.02%)-(-0.37%). Furthermore, the highest survival rate was obtained in treatment A (66.7%) and the lowest in treatment D (33.3%), the highest number of molting lobsters was in treatment A (77.8%) and the lowest was treatment D (33.3%). This shows that the addition of eggshell flour to moist lobster feed has not had a significant effect. For further development, it is necessary to research related to the use of additional ingredients in moist feed for lobster.
The effectiveness of immersed in extract viscera of sea cucumber Holothuria sp on growth, consumption level and feed efficiency in tilapia larvae Oreochromis niloticus Hidayani, Andi Aliah; Achmad, Marlina; Trijuno, Dody Dharmawan; Suwanmala, Jitima; Jannati, Alifah Nurul; Rewa, Atira; Auliah, A. Dyar Fadya
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.148-157

Abstract

The aim of this research was to determine the effect of sea cucumber immersion extract on growth, consumption and feed efficiency in masculinized tilapia larvae aged six to seven days. September–November 2022 was the time the research was carried out and the experimental method used in this study was a Completely Randomized Design (CRD) with five treatments and three replications. The treatment used was the soaking of sea cucumber viscera with doses of 0 ml/L (A), 1 ml/L (B), 3 ml/L for 24 hours (C), 5 ml/L for 24 hours (D), and 0.5 ml/L 17α-MT for 12 hours as positive control (E), then maintained for 60 days. Parameters observed included absolute length growth, absolute weight growth, feed consumption level, feed efficiency and water quality. The results showed that immersion sea cucumber innards for 24 hours with a dose of 1 ml/L showed the highest growth rate in absolute length and a dose of 3 ml/L showed the highest feed consumption rate. Keywords: feed consumption rate, feed efficiency, sea cucumber viscera, tilapia fry ABSTRAK Tujuan dari penelitian ini adalah menentukan pengaruh ekstrak jeroan teripang terhadap pertumbuhan, konsumsi dan efisensi pakan pada larva ikan nila hasil maskulinisasi yang berumur enam sampai tujuh hari. Penelitian ini dilakukan pada bulan September–November 2022 dan metode eksperimen yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah perendaman jeroan teripang dengan dosis 0 ml/L (A), 1 ml/L (B), 3 ml/L (C), 5 ml/L selama 24 jam (D), dan 0,5 ml/L 17α-MT selama 12 jam sebagai kontrol positif (E), kemudian dipelihara selama 60 hari. Parameter yang diamati meliputi pertumbuhan panjang mutlak dan bobot mutlak ikan, tingkat konsumsi pakan, efisiensi pakan dan kualitas air. Hasil penelitian menunjukkan perendaman jeroan teripang selama 24 jam dengan dosis 1 ml/L menunjukkan laju pertumbuhan panjang mutlak yang tertinggi dan pada dosis 3 ml/L tingkat konsumsi pakan dan efsiensi pakan tertinggi. Kata kunci: benih ikan nila, efisiensi pakan, jeroan teripang, tingkat konsumsi pakan
Penyuluhan Metode Budidaya Rumput Laut di Kel. Kalumeme, Kec. Ujungbulu, Kabupaten Bulukumba Badraeni, Badraeni; Hidayani, Andi Aliah; Nurkamilah, Besse Tenri; Trijuno, Dody Dh.; Zainuddin, Elmi N.; Saade, Edison; Latama, Gunarto; Azis, Hasni Y.; Ambas, Irfan; Djawad, M. Iqbal; Achmad, Marlina; Karim, Muh. Yusri; Rustam, Rustam; Aslamyah, Siti; Fujaya, Yushinta; Zainuddin, Zainuddin
Bambu Laut: Jurnal Pengabdian Masyarakat VOLUME 2, NOMOR 1, APRIL 2025
Publisher : Fakultas Ilmu Kelautan dan Perikanan Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35911/bambulaut.v2i1.42271

Abstract

Kecamatan Ujungbulu merupakan salah satu sentra rumput laut di Kab. Bulukumba yang memberikan peluang usaha yang sangat baik bagi peningkatan perekonomian keluarga. Keberhasilan budidaya rumput laut sangat dipengaruhi oleh musim, teknik dan metode budidaya yang tepat sehingga dapat memberikan pertumbuhan yang maksimal seperti pola tanam yang sesuai, penerapan metode tali bentang atau long line. Namun dalam perkembangan metode ini, Masih banyak masalah yang dihadapi oleh pembudidaya, seperti bobot awal bibit yang terlalu besar, jumlah bentangan per awal penanaman per areal yang terlalu banyak, dilakukan penanaman awal yang bersamaan dan penggunaan areal budidaya secara bersamaan per kelompok petani budidaya. Melalui program pengabdian kepada masyarakat dosen PS. BDP, maka dilakukan Penyuluhan Metode Budidaya Rumput Laut. Kegiatan ini dilakukan di Kantor Lurah Kalumeme, Kecamatan Ujungbulu, Kabupaten Bulukumba pada Sabtu 26 Oktober 2024. Sebanyak 30 peserta ikut dalam kegiatan ini yang berasal dari Gapoktan, penyuluh dan dari Dinas Perikanan Kab. Bulukumba. Materi penyuluhan yang disampaikan dipahami dengan baik oleh peserta melalui kegiatan diskusi setelah pemaparan materi.