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All Journal EKSAKTA: Journal of Sciences and Data Analysis Pharmaciana: Jurnal Kefarmasian Jurnal Farmasi Klinik Indonesia Jurnal Ilmiah Kedokteran Wijaya Kusuma Universa Medicina Smart Medical Journal JK Unila (Jurnal Kedokteran Universitas Lampung) Jurnal Teknologi Kesehatan JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Biomedika
Ety Sari Handayani
Departement Of Anatomy, Medical Faculty, Universitas Islam Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Universa Medicina

 1 
Black sugarcane decoction reduces rat brain ischemia Handayani, Ety Sari; Nugraha, Zainuri Sabta; Nurmasitoh, Titis; Kuswati, Kuswati; Ahsani, Dwi N.; Nanda, Ajeng G.
Universa Medicina Vol 35, No 1 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.40-45

Abstract

BackgroundThere are people in Yogyakarta, who use black sugarcane decoction (BSD) to prevent stroke. BSD contains policosanol and antioxidants. It has been proven that policosanol can reduce global ischemia in Mongolian gerbils. This study aims to evaluate the effect of BSD on brain ischemia in a rat stroke model. MethodsA laboratory experiment using eighteen 3-month old male Wistar rats without any defects, of 175-250 g body weight. Brain ischemia was produced by a 20-minute bilateral carotid communis artery oclusion (BCCAO).  Using a rat stroke model, brain ischemia was produced by a 20-minute BCCAO. The rats were randomized into three groups: BSD treated stroke model rats (group 1), non treated stroke model rats (group 2), and sham operated rats (group 3). BSD was administered by gavage for 1 week before BCCAO. Decapitation of rats was performed two hours post BCCAO. Brain tissues were stained with 2,3,5-triphenyltetrazolium chloride (TTC). Ischemic areas were analyzed using Image J softwere. Statistical analysis was conducted by one way ANOVA test.ResultsThe mean percentages of rat brain ischemic area differed between group 3 (0.0 ± 0.0%), group 2 (3.13 ± 0.59%) and group 1 (1.15 ± 0.47%) p =0.001). Post hoc test showed that there was no difference between group 3 with group 1. Instead, there was a significant difference between  group 2 and the other groups.ConclusionThe administration of BSD reduced rat brain ischemia after bilateral carotid artery ligation.
Propolis increases neuronal count in hippocampal area CA1 and prefrontal cortex in stressed rats Nugroho, Kuswati; Handayani, Ety Sari; Nugraha, Zainuri Sabta
Universa Medicina Vol 36, No 3 (2017)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2017.v36.214-220

Abstract

Background Stress induces neuronal cell damage in the hippocampus and prefrontal cortex. Propolis has a neuroprotective effect that can inhibit apoptosis and decrease neuronal cell count. This study aimed to determine the effect of propolis on neuronal cell count in hippocampal area CA1 and prefrontal cortex in Sprague Dawley rats with induced stress.MethodsA study of laboratory experimental design was conducted involving 24 male Sprague-Dawley Rattus norvegicus. The animals were randomly divided into 4 groups, i.e. controls (K), and stress groups P1, P2 and P3. Controls did not receive treatment, stress group (P1) received stress treatment, groups P2 and P3 received stress and propolis at 100 and 200 mg/kgBW, respectively. Stress and propolis were given for 14 days, followed by termination. The number of neurons in the hippocampal area CA1 and prefrontal cortex were counted. One way ANOVA was used to analyze the data.Results The neuronal count in the hippocampal area CA1 and prefrontal cortex in the stress group (P1) was lower than in groups K, P2 and P3. There were significant differences in the neuronal count of the hippocampal area CA1 between P1 and P3 and P1 and K (p=0.019) and also in the neuronal count of the prefrontal cortex between P1 and P2, P3 and K (p=0.002).Conclusions This study strongly suggest that propolis inhibits the decrease in neuronal count in in the hippocampal area CA1 and prefrontal cortex of Sprague Dawley rats with induced stress. The present study suggests a potential neuroprotective effect of propolis in the prevention of neurodegenerative disorders.
Propolis inhibited Bax expression and increased neuronal count of hippocampal area CA1 in rats receiving sodium nitrite Kuswati, Kuswati; Handayani, Ety Sari; Nugraha, Zainuri Sabta; Rahmanti, Fishella Aprista; Wicaksana, Zulfikar Loka; Zhafirrahman, Muhammad
Universa Medicina Vol 38, No 2 (2019)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.491 KB) | DOI: 10.18051/UnivMed.2019.v38.73-80

Abstract

BackgroundSodium nitrite induces hypoxia and oxidative stress in the hippocampus, decreasing the number of neurons in the hippocampus and cognitive function. Propolis contains chrysin that has antioxidant effects that are expected to inhibit neuronal damage in the hippocampus. This study aims to determine the effects of propolis on the expression Bcl-2-associated X protein (Bax) and the number of neurons in the rat hippocampus receiving sodium nitrite.MethodsThis study of laboratory experimental design was conducted on 18 male Wistar strain rats (Rattus norvegicus), they were randomized into 3 groups: one control group (K) received sodium nitrite and two intervention groups  (P1 and P2) received sodium nitrite and propolis at doses of 100 and 200 mg/kgBW. Treatment with sodium nitrite and propolis were given for 60 days, followed by termination. The number of neurons and Bax expression in the hippocampal CA1 area were measured. One-way ANOVA was used to analyze the data.ResultsThere were significant differences in Bax expression between group K and groups P1 and P2 (p<0.001). The lowest number of neurons in the hippocampal CA1 area was in the K group. There were significant differences in the number of neurons between control (K) group and groups P1 and P2 (p<0.001).ConclusionPropolis inhibited the expression of Bax and decreased the number of neurons in the hippocampal CA1 area of rats receiving sodium nitrite. This study provides information about the benefits of propolis as an antioxidant in the brain.
Annona muricata aqueous extract suppresses T47D breast cancer cell proliferation Ika Fidianingsih; Ety Sari Handayani
Universa Medicina Vol. 33 No. 1 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.19-26

Abstract

BackgroundCancer is a dreadful disease caused by abnormal and uncontrolled cell division. Annona muricata L, also known as soursop, is useful as an anticancer herbal medication since its leaves, seeds and fruits contain active compounds called annonaceous acetogenins. The objective of this study was to scientifically justify the traditional application of soursop for anticancer treatment in the community, by comparing the antiproliferative effect of Annona muricata L leaf, seed and fruit aqueous extracts on T47D breast cancer cells. Methods     This study used an experimental post test trial with control group design. Infusions of soursop leaves, seeds, and fruits collected from Kaliurang, Sleman district, Yogyakarta were used for cytotoxicity tests on T47D cells, in comparison with tamoxifen as standard cancer therapy. Proliferative inhibition was determined by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide [MTT] assay. The parameter of proliferative inhibition was IC50 which is defined as 50% proliferative inhibition ability of soursop and tamoxifen. Significant differences between groups were determined at p<0.05 by Kruskal-Wallis test. ResultsThe leaves, fruits, and seeds Annona muricata and tamoxifen were proven to be able to inhibit T47D cell proliferation. The IC50 of Annona muricata leaf, seed, fruit aqueous extracts and tamoxifen were 31,384.21 µg/ml; 1.528,800 µg/ml; 329,194.81 µg/ml and 114.52 µg/ml, respectively (p=0.016). The IC50 of Annona muricata aqueous extract was significantly different from that of tamoxifen.ConclusionsThe proliferative inhibition of soursop leaves against T47D breast cancer cells is higher than that of soursop fruits and seeds. Annona muricata fruit, seed, and leaf aqueous extracts were less toxic than tamoxifen
Co-Authors Adi Nugraha Adyaksa, Dewa Nyoman Murti Afiqoh, Ainun Nur Ajeng G. Nanda, Ajeng G. Ayu Kurnia Priyantiningrum Chandra Muhammad Rauf Dwi N. Ahsani, Dwi N. Farah Jasmine Dianita Gea Sonia Amanda Hamidah Ikhwan, Salma Nur Ika Fidianingsih Indrayani, Tsaniya Ahda Kuswati Kuswati Kuswati Nugroho, Kuswati Kuswati, Kuswati MARIA BINTANG Miranti Dewi Pramaningtyas Muhammad Yusuf Hisyam Nesti Herennadia Nurmasitoh, Titis Nurul Hidayah NURUL HIDAYAH Nurul Hidayah Rahmanti, Fishella Aprista Rika Yulita Rahmawati Sahdella Sahdella Salsabila Ajeng Untung Widodo Utami Mulyaningrum Wicaksana, Zulfikar Loka Wimpy Wimpy Yuantari, Rahma Yusa Muhammad Thoriq Zainuri Sabta Nugaha Zainuri Sabta Nugraha Zainuri Sabta Nugraha, Zainuri Sabta Zhafirrahman, Muhammad