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All Journal EKSAKTA: Journal of Sciences and Data Analysis Pharmaciana: Jurnal Kefarmasian Jurnal Farmasi Klinik Indonesia Jurnal Ilmiah Kedokteran Wijaya Kusuma Universa Medicina Smart Medical Journal JK Unila (Jurnal Kedokteran Universitas Lampung) Jurnal Teknologi Kesehatan JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Biomedika

Ety Sari Handayani

Departement Of Anatomy, Medical Faculty, Universitas Islam Indonesia

Author-ID : 514550

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Black sugarcane decoction reduces rat brain ischemia Handayani, Ety Sari; Nugraha, Zainuri Sabta; Nurmasitoh, Titis; Kuswati, Kuswati; Ahsani, Dwi N.; Nanda, Ajeng G.
Universa Medicina Vol 35, No 1 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.40-45

BackgroundThere are people in Yogyakarta, who use black sugarcane decoction (BSD) to prevent stroke. BSD contains policosanol and antioxidants. It has been proven that policosanol can reduce global ischemia in Mongolian gerbils. This study aims to evaluate the effect of BSD on brain ischemia in a rat stroke model. MethodsA laboratory experiment using eighteen 3-month old male Wistar rats without any defects, of 175-250 g body weight. Brain ischemia was produced by a 20-minute bilateral carotid communis artery oclusion (BCCAO).Â Using a rat stroke model, brain ischemia was produced by a 20-minute BCCAO. The rats were randomized into three groups: BSD treated stroke model rats (group 1), non treated stroke model rats (group 2), and sham operated rats (group 3). BSD was administered by gavage for 1 week before BCCAO. Decapitation of rats was performed two hours post BCCAO. Brain tissues were stained with 2,3,5-triphenyltetrazolium chloride (TTC). Ischemic areas were analyzed using Image J softwere. Statistical analysis was conducted by one way ANOVA test.ResultsThe mean percentages of rat brain ischemic area differed between group 3 (0.0 Â± 0.0%), group 2 (3.13 Â± 0.59%) and group 1 (1.15 Â± 0.47%) p =0.001). Post hoc test showed that there was no difference between group 3 with group 1. Instead, there was a significant difference betweenÂ group 2 and the other groups.ConclusionThe administration of BSD reduced rat brain ischemia after bilateral carotid artery ligation.

Propolis increases neuronal count in hippocampal area CA1 and prefrontal cortex in stressed rats Nugroho, Kuswati; Handayani, Ety Sari; Nugraha, Zainuri Sabta
Universa Medicina Vol 36, No 3 (2017)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2017.v36.214-220

Background Stress induces neuronal cell damage in the hippocampus and prefrontal cortex. Propolis has a neuroprotective effect that can inhibit apoptosis and decrease neuronal cell count. This study aimed to determine the effect of propolis on neuronal cell count in hippocampal area CA1 and prefrontal cortex in Sprague Dawley rats with induced stress.MethodsA study of laboratory experimental design was conducted involving 24 male Sprague-Dawley Rattus norvegicus. The animals were randomly divided into 4 groups, i.e. controls (K), and stress groups P1, P2 and P3. Controls did not receive treatment, stress group (P1) received stress treatment, groups P2 and P3 received stress and propolis at 100 and 200 mg/kgBW, respectively. Stress and propolis were given for 14 days, followed by termination. The number of neurons in the hippocampal area CA1 and prefrontal cortex were counted. One way ANOVA was used to analyze the data.Results The neuronal count in the hippocampal area CA1 and prefrontal cortex in the stress group (P1) was lower than in groups K, P2 and P3. There were significant differences in the neuronal count of the hippocampal area CA1 between P1 and P3 and P1 and K (p=0.019) and also in the neuronal count of the prefrontal cortex between P1 and P2, P3 and K (p=0.002).Conclusions This study strongly suggest that propolis inhibits the decrease in neuronal count in in the hippocampal area CA1 and prefrontal cortex of Sprague Dawley rats with induced stress. The present study suggests a potential neuroprotective effect of propolis in the prevention of neurodegenerative disorders.

Propolis inhibited Bax expression and increased neuronal count of hippocampal area CA1 in rats receiving sodium nitrite Kuswati, Kuswati; Handayani, Ety Sari; Nugraha, Zainuri Sabta; Rahmanti, Fishella Aprista; Wicaksana, Zulfikar Loka; Zhafirrahman, Muhammad
Universa Medicina Vol 38, No 2 (2019)
Publisher : Faculty of Medicine, Trisakti University

BackgroundSodium nitrite induces hypoxia and oxidative stress in the hippocampus, decreasing the number of neurons in the hippocampus and cognitive function. Propolis contains chrysin that has antioxidant effects that are expected to inhibit neuronal damage in the hippocampus. This study aims to determine the effects of propolis on the expression Bcl-2-associated X protein (Bax) and the number of neurons in the rat hippocampus receiving sodium nitrite.MethodsThis study of laboratory experimental design was conducted on 18 male Wistar strain rats (Rattus norvegicus), they were randomized into 3 groups: one control group (K) received sodium nitrite and two intervention groupsÂ (P1 and P2) received sodium nitrite and propolis at doses of 100 and 200 mg/kgBW. Treatment with sodium nitrite and propolis were given for 60 days, followed by termination. The number of neurons and Bax expression in the hippocampal CA1 area were measured. One-way ANOVA was used to analyze the data.ResultsThere were significant differences in Bax expression between group K and groups P1 and P2 (p<0.001). The lowest number of neurons in the hippocampal CA1 area was in the K group. There were significant differences in the number of neurons between control (K) group and groups P1 and P2 (p<0.001).ConclusionPropolis inhibited the expression of Bax and decreased the number of neurons in the hippocampal CA1 area of rats receiving sodium nitrite. This study provides information about the benefits of propolis as an antioxidant in the brain.

Pengaruh Pemberian Ekstrak Etanol Daun Pegagan (Centella asiatica) terhadap Gambaran Histopatologi Limpa Tikus (Rattus norvegicus) yang diinduksi Sodium Nitrit Sub Akut Afiqoh, Ainun Nur; Fidianingsih, Ika; Handayani, Ety Sari
Jurnal Kedokteran Universitas Lampung Vol 1, No 3 (2017): JK UNILA
Publisher : Fakultas Kedokteran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jk unila.v1i3.1668

Sodium nitrit adalah senyawa garam yang secara luas digunakan sebagai pengawet dan pewarna merah daging. Konsumsi berlebihan dan dalam jangka waktu yang lama dapat menyebabkan terbentuknya senyawa karsinogenik dan terbentuk methemoglobin. Methemoglobin dapat menyebabkan terbentuknya Reactive Oxygen Species (ROS) yang dapat menyebabkan stres oksidatif pada berbagai sel tubuh salah satunya sel limfosit pada pulpa putih limpa. Sel limfosit yang berperan penting dalam sistem imun tubuh ini dapat mengalami nekrosis karena oksidan ini. Pemberian ekstrak etanol daun pegagan diharapkan dapat mencegah efek dari sodium nitrit. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak etanol daun pegagan terhadap gambaran histopatologi limpa tikus yang diinduksi dengan sodium nitrit subakut. Metode penelitian yang digunakan adalah penelitian eksperimental dengan posttest control group design. Sampel dibagi ke dalam 3 kelompok dengan 5 ekor tikus pada masing-masing kelompok. Kelompok tersebutadalah kelompok kontrol, PI (sodium nitrit 50 mg/kgBB), dan PII (sodium nitrit 50 mg/kgBB dan ekstrak etanol daun pegagan 600 mg/kgBB). Setelah perlakuan selama 42 hari, diambil preparat limpa tikus untuk dilihat rata-rata luas pulpa putih. Terdapat perbedaan tidak bermakna rata-rata luas pulpa putih pada setiap kelompok dengan rata-rata terkecilterdapat pada kelompok PI (p=0,899). Ekstrak etanol daun pegagan tidak berpengaruh secara bermakna terhadap gambaran histopatologi limpa yang diinduksi sodium nitrit subakut.Kata kunci: limpa, pegagan, pulpa putih, sodium nitrit

Pengaruh Durasi Iskemia terhadap Ekspresi Protein P53 Otak Tikus Pasca Transient Bilateral Common Carotid Artery Occlusion (TBCCAO) Ety Sari Handayani; Kuswati Kuswati; Zainuri Sabta Nugaha; Nurul Hidayah; Nesti Herennadia; Gea Sonia Amanda; Salsabila Ajeng
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 19, ISSUE 2, August 2019
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol19.iss2.art3

Tumor suppressor gene p53 is one of the specific parameters of the occurrence of brain neuron death in animal models of brain ischemia. Several studies show that the duration of reperfusion of tBCCAO has an effect on the expression of p53 protein. The duration of ischemia for 5 minutes followed by reperfusion of 1 hour, 4 hours, 8 hours, 24 hours, and 7 days increased the expression of p53 protein. 30-minute ischemia induction followed by 4-hour reperfusion showed increased p53 protein expression in rat serum. It is not yet known how the influence of tBCCAO ischemia duration on p53 expression in rat brain. This study used Wistar, male rats, aged 3-4 months, weighing 175-250 gr, and healthy. Group A was a group of tBCCAO rats with a duration of 5 minutes ischemia, 24-hour reperfusion duration (five). Group B was a group of tBCCAO mice with a duration of 10 minutes ischemia, 24-hour reperfusion (five). Group C is a group of sham operated mice (five). The expression of p53 protein is semi quantification of p53 expressed on pyramidal neuron of the frontal brain (Cortex Prefrontalis, striatum) and CA1 hippocampus, with IHC staining using anti-p53 antibodies. P53 expression will be seen in the cytoplasm of pyramidal neuron CA1 hippocampus. Cytoplasm of neurons will be brownish in color. The semi-quantification method of p53 expression uses the ALLRED score. Data analysis using one way ANOVA test. The analysis found differences in frontal brain p53 expression (cortex prefrontalis and striatum) (p = 0.00) and there were differences in p53 expression in pyramidal neuron CA1 hippocampal (p = 0.013). There was an effect of the duration of tBCCAO on expression of p53 protein in rat brain after 24-hour reperfusion. Keywords: tBCCAO’s duration, p53, rat’s brain

P53 expression in ischemic rat models after the administration of ketamine and ketamine-xylazine Ety Sari Handayani; Zainuri Sabta Nugraha; Kuswati Kuswati; Muhammad Yusuf Hisyam; Untung Widodo; Nurul Hidayah; Sahdella Sahdella; Wimpy Wimpy
Pharmaciana Vol 10, No 1 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Ketamine and ketamine-xylazine are often used as anesthetic drugs in animal models of ischemia. However, their neuroprotective and neurotoxic effects in ischemic animal models that have undergone tBCCAO are still under debate. The protein p53 is a pro-apoptotic factor involved in the cellular mechanism of ischemia. The interaction between death-associated protein kinase 1 (DAPK 1) and p53 is fundamental in determining whether cells experience necrosis or apoptosis in an ischemic stroke. This study was purposed to identify the presence or absence of differences between the p53 expressions in the brains of tBCCAO-induced ischemic rat models after the administration of ketamine and ketamine-xylazine. It employed a post-test control group design with four groups of adult male Wistar rats as the subject: (1) sham group operated with ketamine, (2) sham group operated with ketamine-xylazine, (3) models of tBCCAO-induced ischemia with ketamine, and (4) models of tBCCAO-induced ischemia with ketamine-xylazine. Ketamine was administered at the dose of 75mg/kg BW, while xylazine was at 8 mg/kg BW. The expression of p53 in rat brains was assessed by semi-quantification, specifically IHC staining with anti-p53 antibodies. P53 expression appeared as brownish stains in the cytoplasm of forebrain pyramidal neurons, and in this study, it was measured using the Allred score. The ANOVA test yielded a p-value of >0.05, implying the absence of difference between the p53 expressions in the brains of tBCCAO-induced ischemic rat models receiving ketamine and ketamine-xylazine.

Annona muricata aqueous extract suppresses T47D breast cancer cell proliferation Ika Fidianingsih; Ety Sari Handayani
Universa Medicina Vol. 33 No. 1 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.19-26

BackgroundCancer is a dreadful disease caused by abnormal and uncontrolled cell division. Annona muricata L, also known as soursop, is useful as an anticancer herbal medication since its leaves, seeds and fruits contain active compounds called annonaceous acetogenins. The objective of this study was to scientifically justify the traditional application of soursop for anticancer treatment in the community, by comparing the antiproliferative effect of Annona muricata L leaf, seed and fruit aqueous extracts on T47D breast cancer cells. Methods This study used an experimental post test trial with control group design. Infusions of soursop leaves, seeds, and fruits collected from Kaliurang, Sleman district, Yogyakarta were used for cytotoxicity tests on T47D cells, in comparison with tamoxifen as standard cancer therapy. Proliferative inhibition was determined by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide [MTT] assay. The parameter of proliferative inhibition was IC50 which is defined as 50% proliferative inhibition ability of soursop and tamoxifen. Significant differences between groups were determined at p<0.05 by Kruskal-Wallis test. ResultsThe leaves, fruits, and seeds Annona muricata and tamoxifen were proven to be able to inhibit T47D cell proliferation. The IC50 of Annona muricata leaf, seed, fruit aqueous extracts and tamoxifen were 31,384.21 µg/ml; 1.528,800 µg/ml; 329,194.81 µg/ml and 114.52 µg/ml, respectively (p=0.016). The IC50 of Annona muricata aqueous extract was significantly different from that of tamoxifen.ConclusionsThe proliferative inhibition of soursop leaves against T47D breast cancer cells is higher than that of soursop fruits and seeds. Annona muricata fruit, seed, and leaf aqueous extracts were less toxic than tamoxifen

Pengaruh Durasi DM Tipe 2 Terhadap Angka Leukosit dan Hitung Jenis Leukosit Pada Tikus Wistar Pasca Bilateral Common Carotid Artery Occlusion (BCCAO) Ety Sari Handayani; Farah Jasmine Dianita; Rahma Yuantari Yuantari
Smart Medical Journal Vol 5, No 1 (2022): Smart Medical Journal
Publisher : Faculty of Medicine Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/smj.v5i1.42787

Pendahuluan: Stroke iskemik merupakan masalah kesehatan yang sering ditemui di dunia terutama Indonesia. BCCAO merupakan teknik yang dapat digunakan untuk menginduksi iskemia serebral. Salah satu faktor risiko stroke iskemik yaitu diabetes melitus tipe 2 dimana dapat meningkatkan agregasi leukosit dan aktivasi aterosklerosis. Mengetahui pengaruh durasi diabetes melitus tipe 2 terhadap angka leukosit dan hitung jenis leukosit pada tikus pasca ligasi BCCAO.Metode: Jenis penelitian berupa kuasi eksperimental. Subjek penelitian ini yaitu tikus jantan dewasa (Rattus norvegicus) galur Wistar. Tikus dikelompokkan menjadi 4 kelompok perlakuan, masing-masing terdiri dari 6 ekor tikus. Kelompok 1 merupakan kelompok sham operated, kelompok 2 merupakan kelompok iskemik (diligasi BCCAO selama 20 menit dan reperfusi 7 hari), kelompok 3 merupakan kelompok perlakuan DM-iskemik (DM selama 2 minggu dengan BCCAO), dan kelompok 4 merupakan kelompok DM-iskemik (DM selama 3 minggu dengan BCCAO. Analisis data menggunakan uji ANOVA dan Kruskal Wallis.Hasil: Angka leukosit dan hitung jenis leukosit pada DMT2 durasi 3 minggu memiliki nilai Neutrofil yang signifikan (23,53x 5,57; p = 0,014, p <0,05) dan Limfosit (66,68 x 6,16; p = 0,028, p <0,05) setelah induksi stroke iskemik.Kesimpulan: Terdapat pengaruh durasi diabetes melitus tipe 2 terhadap hitung jenis leukosit yaitu neutrofil dan limfosit pada tikus pasca ligasi BCCAO.Kata Kunci: Durasi DM, BCCAO, angka leukosit, hitung jenis leukosit

Routine Blood Profiles of Global Ischemic Rats Based on Ischemia Durations Rika Yulita Rahmawati; Utami Mulyaningrum; Ety Sari Handayani
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 3, ISSUE 1, February 2022
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol3.iss1.art6

Stroke is the third cause of death and the first cause of disability in the world. It is around 80% of stroke patients in the world are ischemic stroke. According to the development of stroke models in animals, BCCAO is one technique that can induce global cerebral ischemia. An ischemia is known to influence activities of inflammatory cells which can be measured through peripheral blood. This study aims to determine effects of ischemia duration on routine blood profiles of rats (Rattus norvegicus) after bilateral common carotid artery occlusion (BCCAO). This study was a quasi-experimental study. Its subjects were adult Wistar rats (Rattus norvegicus). The rats were grouped into four treatment groups, and each group consisted of 6 rats. Group A was a group of sham-operated rats, group B was a group of rats with ischemia duration for 5 minutes, group C was a group of rats with ischemia duration for 10 minutes, and group D was a group of rats with ischemia duration for 20 minutes. Its obtained data were analysed by one-way ANOVA test and Post Hoc Tamhane's test. The ischemia duration significantly influenced the neutrophil and lymphocyte count after ischemia, with p < 0.05. The ischemia duration could affect the routine blood profiles of the rats after BCCAO, especially the neutrophil and lymphocyte count.

The Effect of Subchronic Administration of Soursop (Annona muricata) Leaf Aqueous Extract In Bax Expression on Gastric Glandular and Non Glandular Mucosal Epithelium of Rat (Rattus norvegicus) Ety Sari Handayani; Zainuri Sabta Nugraha; Chandra Muhammad Rauf; Yusa Muhammad Thoriq; Nurul Hidayah
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 3, ISSUE 1, February 2022
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol3.iss1.art7

Soursop leaf water extract (SLWE) contains acetogenin compounds which are mitochondrial complex I inhibitors. This compound can reduce cellular ATP production and induce apoptosis via bax pathways, causing side effects on cells. Gaster is a digestive organ that has direct contact with acetogenin. Gastric cells contain mitochondria and undergo physiological apoptosis.This study aims to determine the effect of subchronic administration of SLWE on bax expression on glandular/non glandular mucosal epithelium gastric of rats. This study was conducted at Research Laboratorium, Medical Faculty, UII, 2019. This study uses post test only control group design. The subjects were 10 female rats, Spraque-dawley strain which was divided into 2 groups; the treatment and the control group. The treatment group received extract of SLWE at a dose of 1000 mg/kgBB/day for 30 days, while the control group received aquades, both were administered using sonde. Observation of bax expression was performed on each IHC preparation with bax antibody. The difference in bax expression between the control and treatment groups was tested by t-test. There were significant differences in the number of bax expressions in gastric glandular mucosa (p 0.038) and non-glandular gastric mucosa (p 0.027) between the treatment group and the control group. There was an effect of subchronic administration of SLWE on bax expression on mucosalepithelium for both, glandular and non-glandular of rat gaster. There were differences in the number of glandular gastric as well as non-glandular gastric mucosa epithelium which exerted bax between the control and treatment groups

Co-Authors Adi Nugraha Adyaksa, Dewa Nyoman Murti Afiqoh, Ainun Nur Ajeng G. Nanda, Ajeng G. Ayu Kurnia Priyantiningrum Chandra Muhammad Rauf Dwi N. Ahsani, Dwi N. Farah Jasmine Dianita Gea Sonia Amanda Hamidah Ikhwan, Salma Nur Ika Fidianingsih Indrayani, Tsaniya Ahda Kuswati Kuswati Kuswati Nugroho, Kuswati Kuswati, Kuswati MARIA BINTANG Miranti Dewi Pramaningtyas Muhammad Yusuf Hisyam Nesti Herennadia Nurmasitoh, Titis NURUL HIDAYAH Nurul Hidayah Nurul Hidayah Rahmanti, Fishella Aprista Rika Yulita Rahmawati Sahdella Sahdella Salsabila Ajeng Untung Widodo Utami Mulyaningrum Wicaksana, Zulfikar Loka Wimpy Wimpy Yuantari, Rahma Yusa Muhammad Thoriq Zainuri Sabta Nugaha Zainuri Sabta Nugraha Zainuri Sabta Nugraha, Zainuri Sabta Zhafirrahman, Muhammad

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