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All Journal EKSAKTA: Journal of Sciences and Data Analysis Pharmaciana: Jurnal Kefarmasian Jurnal Farmasi Klinik Indonesia Jurnal Ilmiah Kedokteran Wijaya Kusuma Universa Medicina Smart Medical Journal JK Unila (Jurnal Kedokteran Universitas Lampung) Jurnal Teknologi Kesehatan JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Biomedika
Ety Sari Handayani
Departement Of Anatomy, Medical Faculty, Universitas Islam Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : EKSAKTA: Journal of Sciences and Data Analysis

 1 
Pengaruh Durasi Iskemia terhadap Ekspresi Protein P53 Otak Tikus Pasca Transient Bilateral Common Carotid Artery Occlusion (TBCCAO) Ety Sari Handayani; Kuswati Kuswati; Zainuri Sabta Nugaha; Nurul Hidayah; Nesti Herennadia; Gea Sonia Amanda; Salsabila Ajeng
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 19, ISSUE 2, August 2019
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol19.iss2.art3

Abstract

Tumor suppressor gene p53 is one of the specific parameters of the occurrence of brain neuron death in animal models of brain ischemia. Several studies show that the duration of reperfusion of tBCCAO has an effect on the expression of p53 protein. The duration of ischemia for 5 minutes followed by reperfusion of 1 hour, 4 hours, 8 hours, 24 hours, and 7 days increased the expression of p53 protein. 30-minute ischemia induction followed by 4-hour reperfusion showed increased p53 protein expression in rat serum. It is not yet known how the influence of tBCCAO ischemia duration on p53 expression in rat brain. This study used Wistar, male rats, aged 3-4 months, weighing 175-250 gr, and healthy. Group A was a group of tBCCAO rats with a duration of 5 minutes ischemia, 24-hour reperfusion duration (five). Group B was a group of tBCCAO mice with a duration of 10 minutes ischemia, 24-hour reperfusion (five). Group C is a group of sham operated mice (five). The expression of p53 protein is semi quantification of p53 expressed on pyramidal neuron of the frontal brain (Cortex Prefrontalis, striatum) and CA1 hippocampus, with IHC staining using anti-p53 antibodies. P53 expression will be seen in the cytoplasm of pyramidal neuron CA1 hippocampus. Cytoplasm of neurons will be brownish in color. The semi-quantification method of p53 expression uses the ALLRED score. Data analysis using one way ANOVA test. The analysis found differences in frontal brain p53 expression (cortex prefrontalis and striatum) (p = 0.00) and there were differences in p53 expression in pyramidal neuron CA1 hippocampal (p = 0.013). There was an effect of the duration of tBCCAO on expression of p53 protein in rat brain after 24-hour reperfusion. Keywords: tBCCAO’s duration, p53, rat’s brain
Routine Blood Profiles of Global Ischemic Rats Based on Ischemia Durations Rika Yulita Rahmawati; Utami Mulyaningrum; Ety Sari Handayani
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 3, ISSUE 1, February 2022
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol3.iss1.art6

Abstract

Stroke is the third cause of death and the first cause of disability in the world. It is around 80% of stroke patients in the world are ischemic stroke. According to the development of stroke models in animals, BCCAO is one technique that can induce global cerebral ischemia. An ischemia is known to influence activities of inflammatory cells which can be measured through peripheral blood. This study aims to determine effects of ischemia duration on routine blood profiles of rats (Rattus norvegicus) after bilateral common carotid artery occlusion (BCCAO). This study was a quasi-experimental study. Its subjects were adult Wistar rats (Rattus norvegicus). The rats were grouped into four treatment groups, and each group consisted of 6 rats. Group A was a group of sham-operated rats, group B was a group of rats with ischemia duration for 5 minutes, group C was a group of rats with ischemia duration for 10 minutes, and group D was a group of rats with ischemia duration for 20 minutes. Its obtained data were analysed by one-way ANOVA test and Post Hoc Tamhane's test. The ischemia duration significantly influenced the neutrophil and lymphocyte count after ischemia, with p < 0.05. The ischemia duration could affect the routine blood profiles of the rats after BCCAO, especially the neutrophil and lymphocyte count.
The Effect of Subchronic Administration of Soursop (Annona muricata) Leaf Aqueous Extract In Bax Expression on Gastric Glandular and Non Glandular Mucosal Epithelium of Rat (Rattus norvegicus) Ety Sari Handayani; Zainuri Sabta Nugraha; Chandra Muhammad Rauf; Yusa Muhammad Thoriq; Nurul Hidayah
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 3, ISSUE 1, February 2022
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol3.iss1.art7

Abstract

Soursop leaf water extract (SLWE) contains acetogenin compounds which are mitochondrial complex I inhibitors. This compound can reduce cellular ATP production and induce apoptosis via bax pathways, causing side effects on cells. Gaster is a digestive organ that has direct contact with acetogenin. Gastric cells contain mitochondria and undergo physiological apoptosis.This study aims to determine the effect of subchronic administration of SLWE on bax expression on glandular/non glandular mucosal epithelium gastric of rats. This study was conducted at Research Laboratorium, Medical Faculty, UII, 2019. This study uses post test only control group design. The subjects were 10 female rats, Spraque-dawley strain which was divided into 2 groups; the treatment and the control group. The treatment group received extract of SLWE at a dose of 1000 mg/kgBB/day for 30 days, while the control group received aquades, both were administered using sonde. Observation of bax expression was performed on each IHC preparation with bax antibody. The difference in bax expression between the control and treatment groups was tested by t-test. There were significant differences in the number of bax expressions in gastric glandular mucosa (p 0.038) and non-glandular gastric mucosa (p 0.027) between the treatment group and the control group. There was an effect of subchronic administration of SLWE on bax expression on mucosalepithelium for both, glandular and non-glandular of rat gaster. There were differences in the number of glandular gastric as well as non-glandular gastric mucosa epithelium which exerted bax between the control and treatment groups
Co-Authors Adi Nugraha Adyaksa, Dewa Nyoman Murti Afiqoh, Ainun Nur Ajeng G. Nanda, Ajeng G. Ayu Kurnia Priyantiningrum Chandra Muhammad Rauf Dwi N. Ahsani, Dwi N. Farah Jasmine Dianita Gea Sonia Amanda Hamidah Ikhwan, Salma Nur Ika Fidianingsih Indrayani, Tsaniya Ahda Kuswati Kuswati Kuswati Nugroho, Kuswati Kuswati, Kuswati MARIA BINTANG Miranti Dewi Pramaningtyas Muhammad Yusuf Hisyam Nesti Herennadia Nurmasitoh, Titis Nurul Hidayah NURUL HIDAYAH Nurul Hidayah Rahmanti, Fishella Aprista Rika Yulita Rahmawati Sahdella Sahdella Salsabila Ajeng Untung Widodo Utami Mulyaningrum Wicaksana, Zulfikar Loka Wimpy Wimpy Yuantari, Rahma Yusa Muhammad Thoriq Zainuri Sabta Nugaha Zainuri Sabta Nugraha Zainuri Sabta Nugraha, Zainuri Sabta Zhafirrahman, Muhammad