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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology TEGAR: Journal of Teaching Physical Education in Elementary School Mitra Akademia: Jurnal Pengabdian Masyarakat Makara Journal of Science Environmental and Materials Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Endang Saepudin, Endang
Chemistry Department, Faculty of Mathematics and Science, University of Indonesia, Depok 16424, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Physics Social Sciences

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Journal : Makara Journal of Science

 1 
Identification and Bioactivity Studies of Flavonoid Compounds from Macaranga hispida (Blume) Mull.Arg Megawati,; Saepudin, Endang; Hanafi, Muhammad; Darmawan, Akhmad; Lotulung, Puspa Dewi N.
Makara Journal of Science Vol. 19, No. 3
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Two flavonoid compounds, 5,7,3’,4’-tetrahydroxy-6-geranylflavonol (1) and kaempferol 7-O-β-glucose (2) have been isolated from the leaves of Macaranga hispida (Blume), Mull.Arg. Isolation and purification were conducted by chromatography methods and chemical structure characterization was carried out by spectroscopic methods. The 5,7,3’,4’-tetrahydrxyi-6-geranyl flavonol (1) and kaempferol 7-O-glucose (2) had moderate cytotoxic activity against murine leukemia P-388 cell lines with IC50 value of 0.22 and 101.5 μg/mL, respectively. The IC50 for antioxidant activities of (1) and (2) were 2.83 and 13.95 μg/mL, respectively. The LC50 of (1) and (2) from BSLT were 350 and >1000 μg/mL, respectively.
Enzymatic Hydrolysis of Mannan from Konjac (Amorphophallus sp.) Using Mannanase from Streptomyces lipmanii to Produce Manno-oligosaccharides Sasongko, Ashadi; Yopi,; Rahmani, Nanik; Lisdiyanti, Puspita; Saepudin, Endang
Makara Journal of Science Vol. 19, No. 3
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Mannan is an abundant polysaccharide that can be found in konjac (Amorphophallus sp.). Mannan can be enzymatically hydrolyzed using mannanase to produce manno-oligosaccharides which can be used as a prebiotic. The aims of this research are to determine the production time of mannanase from Streptomyces lipmanii, perform enzyme characterization, optimize the hydrolysis time, and characterize the hydrolysis product. A qualitative assay using the indicator Congo red showed that S. lipmanii generated a clear zone, indicating that S. lipmanii produced mannanase in konjac medium and possessed mannanolytic activity. Enzyme activity was determined through reducing sugar measurement using the dinitrosalycylic acid method, and optimum enzyme production was achieved at the second day of culture. Characterization of the enzyme showed that hydrolysis was optimum at pH 7 and at a temperature of 50 oC. The reducing sugar content was increased by an increasing the hydrolysis time, and reached an optimum time at 2 h. The degree of polymerization value of three was achieved after 2 h hydrolysis of mannan from konjac, indicating the formation of oligosaccharides. Analysis by thin layer chromatography using butanol, acetic acid, and water in a ratio of 2:1:1 as eluent showed the presence of compounds with a retention time between those of mannose and mannotetrose. Confirmation was also performed by HPLC, based on the retention time.
Electrochemical Detection of Neuraminidase Based on Zanamivir Inhibition Reaction at Platinum and Platinum-Modified Boron-Doped Diamond Electrodes Ivandini, Tribidasari Anggraningrum; Ariani, Jenny; Jiwanti, Prastika Krisma; Gunlazuardi, Jarnuzi; Saepudin, Endang; Einaga, Yasuaki
Makara Journal of Science Vol. 21, No. 1
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Neuraminidase sensors have been developed using platinum (Pt) and platinum-modified boron-doped diamond (Pt-BDD) electrodes based on the difference of electrochemical responses of zanamivir in the absence and in presence of neuraminidase. Cyclic voltammetry of zanamivir showed that the presence of zanamivir decreases the oxidation-reduction peak currents of Pt. The responses were found to be linear in the zanamivir concentration range of 7.5-150 μM. Comparison between Pt-BDD and Pt bulk electrodes showed that Pt-BDD offers approximately two times higher sensitivity of zanamivir responses than Pt bulk. Further comparison to gold-based electrodes showed that Pt-based electrodes provided about one order higher sensitivity of zanamivir responses. In the presence of neuraminidase, the oxidation-reduction currents of Pt again increase. Observation on the oxidation currents in the system with 2 x 10-5 M zanamivir showed that the responses were linear in the neuraminidase concentration range of 5-20 mU. Good reproducibility with an RSD < 3 (n = 10) was achieved even when the measurements were performed in a mucin matrix,suggesting that Pt-based electrodes are suitable to be applied for the detection method of neuraminidase.
Hemoglobin-Modified Core–Shell Fe3O4@Au Nanostructures for the Electrochemical Detection of Acrylamide Saepudin, Endang; Yuliani, Tri; Nasution, Mochammad Arfin Fardiansyah; Khalil, Munawar; Hong, Jong Wook; Ivandini, Tribidasari Anggraningrum
Makara Journal of Science Vol. 25, No. 3
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In this study, electrochemical detection of acrylamide using hemoglobin (Hb)-modified core–shell Fe3O4@Au nanostructures was conducted. Fe3O4 nanoparticles (~4.9 nm) and core–shell Fe3O4@Au (5.0–6.4 nm) nanostructures were successfully synthesized by the thermal decomposition method. Electrochemical investigation revealed that the optimum amount of Hb of 2 mg/mL could be immobilized in 0.1 M acetate buffer solution (pH = 6). Moreover, the detection of acrylamide using Fe3O4@Au/Hb was evaluated by the cyclic voltammetry technique. A linear calibration curve (R2 = 0.98) in the concentration range of 0.1 to 1.0 µM could be achieved with an estimated limit of detection, limit of quantification, and sensitivity of 0.136 µM, 0.453 µM, and 0.4411 µA/µM, respectively. Furthermore, the developed biosensor exhibited high selectivity in the presence of ascorbic acid, melamine, and caffeine. The developed biosensor was applied to the detection of acrylamide in coffee samples and validated using the standard high-performance liquid chromatography (HPLC) method. The concentration of acrylamide in coffee samples was determined to be 37.450 and 35.377 ppm using electrochemical measurement and HPLC, respectively.
Development of Immunochromatographic Strip Tests for Selective and Quantitative Detection of Melamine Wicaksono, Wiyogo P.; Ivandini, Tribidasari A.; Saepudin, Endang; Einaga, Yasuaki
Makara Journal of Science Vol. 18, No. 3
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An immunochromatographic strip test based on the complex reaction of antigen-antibody (melamine-antimelamine) was developed for quantitative detection of melamine. Gold nanoparticles (AuNP) were used to form AuNP-labeled antibody, which then acted as a biosensor. Melamine quantification was performed by the determination of AuNP using anodic stripping voltammetry technique with a boron-doped diamond as the working electrode. With sample volume of 100 µL and immunoreaction time of 7 min, the developed immunochromatographic strip test produced a linear calibration curve for melamine concentration range of 0–0.6 mg/L, with detection limit of 0.1 mg/L and RSD of ~5%. Furthermore, negative results were obtained for samples containing cyanuric acid and urea, indicating that the developed immunochromatographic strip test has potential for selective and quantitative detection of melamine.
THE DEVELOPMENT OF BIOCHEMICAL OXYGEN DEMAND SENSOR USING LOCAL YEAST: Candida fukuyamaensis, UICC Y-247 Anggraningrum, Ivandini Tribidasari; Zulfia, Fenny Triana; Saepudin, Endang
Makara Journal of Science Vol. 15, No. 1
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In order to shorten the measurement time of biochemical oxygen demand (BOD), a BOD sensor based on yeast metabolism was developed. Local yeast, Indonesian Origin, Candida fukuyamaensis UICC Y-247, was used as a transducer. The yeast was immobilized as a thin film in agarose matrix with the auxiliary of Nafion® acting as the membrane for ion exchange process. The film was then attached to gold-modified glassy carbons and used as transducer on the working electrodes. The measurements were conducted by observing the depletion of glucose concentration using multipulse amperometric method and then converted to BOD values. Optimum condition was observed in a waiting measurement time of 30 min at an applied potential of 450 mV (vs. Ag/AgCl). Linearity was shown in glucose concentration range of 0.1–0.5 mM, which was equivalent to BOD concentration range of 10–50 mg/L. A detection limit of 1.13 mg/L BOD could be achieved. Good repeatability was shown by a relative standard deviation (RSD) of 2.7% (n = 15). However, decreasing current response of ~50% was found after 3 days. Comparing to the conventional BOD measurement, this BOD sensor can be used as an alternative method for BOD measurements.
Co-Authors Adiwinata, Syan Rosyid Afiten Rahmin Sanjaya Akhmad Darmawan Akhmad Darmawan Aliyah Ariani, Jenny Ashadi Sasongko Auditia, Ihsan Darwin Darwin Didin Budiman, Didin Einaga, Yasuaki Fatonah, Ani Fatonah Fenny Triana Zulfia Hong, Jong Wook Ivandini Tribidasari Anggraningrum Ivandini, Tribidasari A. Jarnuzi Gunlazuardi Jarnuzi Gunlazuardi Jenny Ariani Lestari K., Sri Lotulung, Puspa Dewi N. Maemonah, Maemonah Megawati Megawati Megawati, Muhammad Hanafi Muhammad Hanafi Munawar Khalil NANIK RAHMANI Nanik Rahmani Nasution, Mochammad Arfin Fardiansyah Nuralam Syamsuddin Nurhalimah, Dede Nurhidayat , Reza Rizqi Prastika Krisma Jiwanti Purwanti, B. S. R. Puspa Dewi N Lotulung PUSPITA LISDIYANTI Putri, Yulia Mariana Tesa Ayudia Qosthalani, Fildzah Alfita RAHMAT WIBOWO Rahmawati, Isnaini Riyanto, Hanzhola Gusman Sanjaya, Afiten Rahmin Sinurat, Ellya Suyanto Syaprudin, Syaprudin Tesla, Yudistira Tri Yuliani, Tri Tribidasari A Ivandini Wahyudi, Widi Wicaksono, Wiyogo P. Wiyogo P Wicaksono Yasuaki Einaga Yasuaki Einaga Yopi Yopi Yopi, Yuni Krisyuningsih Krisnandi, Yuni Krisyuningsih Zulfia, Fenny Triana