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The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model Budi Susilo; Cahya Yustisia Hasan; Bambang Dwi Raharjo; Tri Wahyu Pangestiningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 8 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i8.338

Wound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats model diabetes mellitus type 2.Twenty-four Sprague dawley rats that match the inclusion criteria were made into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then devided into six group: DM3, DM7, DM14, DML3, DML7, DML14, with 4 rats each. All rats were made excision wound with punch biopsy in the back area. DML group receive LIPUS therapy in the wound area (frequency 3 MHz, intensity 0,5 W/cm 2 , duty cycle 20%, duration 3 minutes every day) for three days (DML3), seven days (DML7) and fourteen days (DML14). Wound area was measured and the tissue was staineing with hematoksciline eiosin to observe the density of angiogenesis.Two Way ANOVA used to to analyze the differences in the variable wound area and angiogenesis density, Post Hoc LSD to analyze the differences in variables between groups and the day of observation, showed the area of the wound in the DML was lower than in the DM group significant on day 3 and 7, whereas the density of the angiogenesis in DML group was higher than DM group significant on day 7 and 14. The Pearson test showed a correlation between the wound closure and the angiogenesis density (p = 0,000; r = -0,785). Conclusion: LIPUS therapy accelerate wound closure on day 3 and 7and increase the density of angiogenesis on day 7 and 14.

Penatalaksanaan Cancrum Oris (Noma) Akibat Super Infeksi Jamur pada Anak dengan Myelodysplastic Syndrome Adyaputra Indrapradana; Cahya Yustisia Hasan; Bakhrul Lutfianto
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 6, No 2 (2020)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.54306

Cancrum oris (noma) adalah penyakit gangren orofasial berupa destruksi pada jaringan yang terinfeksi dengan proses yang sangat cepat sehingga dapat menimbulkan mutilasi jaringan dan deformitas fasial. Noma timbul pada anak dengan malnutrisi, kebersihan mulut yang buruk, serta kondisi immunocompromised seperti AIDS, terapi imunosupresif, dan myelodysplastic syndrome sehingga memiliki tingkat morbiditas dan mortalitas yang tinggi. Melaporkan tatalaksana bedah maupun non-bedah pada kasus noma akibat super infeksi jamur pada anak dengan myelodysplastic syndrome. Pasien anak laki-laki usia 9 tahun yang terdiagnosis myelodysplastic syndrome menderita noma dengan gambaran klinis destruksi nekrotik dan ulseratif pada sudut bibir kanan dan kiri yang meluas hingga hampir menutupi seluruh mulut. Pasien dirawat di rumah sakit di bawah perawatan tim multidisiplin yang terdiri dari ahli bedah mulut, dokter anak, dan ahli gizi untuk meningkatkan kondisi kesehatan umumnya. Biopsi eksisi dan debridemen agresif dilakukan. Pemeriksaan mikrobiologi menunjukkan adanya superinfeksi jamur dan diikuti pemberian terapi antibiotik dan antijamur untuk mencegah kerusakan lebih lanjut. Pasien dinyatakan sembuh dengan ditandai penyembuhan luka yang baik dan tidak terjadi rekurensi setelah evaluasi 3 bulan pasca perawatan. Penanganan noma pada anak dengan myelodysplastic syndrome memerlukan tatalaksana bedah maupun non-bedah dengan disiplin dan tanggung jawab yang tinggi agar kerusakan jaringan tidak semakin berlanjut.

Effect of low-intensity pulsed ultrasound on mast cell degranulation and fibroblast expression on type 2 diabetes mellitus rats wound healing process Intan Maharani; Cahya Yustisia Hasan; Bambang Dwirahardjo; Tri Wahyu Pangestiningsih
Majalah Kedokteran Gigi Indonesia Vol 7, No 2 (2021): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.59132

Impaired wound healing is one of the Diabetes mellitus complications. Low-intensity Pulsed Ultrasound (LIPUS) therapy may accelerate the impaired wound healing. The use of LIPUS therapy in the early inflammatory phase can induce mast cell degranulation, and in the proliferative phase it can increase collagen synthesis by fibroblasts. The purpose of this study was to determine the effects of LIPUS therapy on mast cell degranulation and fibroblastexpression in the healing process of punch biopsy wound in rats with type 2 diabetes mellitus. Twenty-four Sprague dawley (n=24) were designed into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then divided into six groups: diabetes mellitus without LIPUS (DM3, DM7, DM14) and diabetes mellitus with LIPUS (DML3, DML7, DML14), 4 each, and punch biopsy wounds were made on the dorsal skin. The DML group received LIPUS therapy in the wound area (frequency 3 MHz, intensity 0.5 W/cm2, duty cycle 20%, duration 3 minutes every day for 3 days (DML3), 7 days (DML7), and 14 days (DML14). The wounded tissue area was stained with toluidine blue to observe mast cell degranulation and immunohistochemical type HSP-47 to observe fibroblast expression. Two-Way ANOVA and Post Hoc LSD tests were used to determine the differences in mast cell degranulation and fibroblast expression. The results showed that mast cell degranulation and fibroblast expression in the DML group were higher than in the DM group (table 1). Pearson test showed a correlation between mast cell degranulation and fibroblast expression (p=0.00; r= 0.839). LIPUS therapy increases mast cell degranulation and fibroblast expression in type 2 diabetes mellitus rat model. The higher the mast cell degranulation, the higher fibroblast expressions.

PERAWATAN FIBROUS DYSPLASIA MAKSILA DEXTRA ANTERIOR PADA ANAK USIA 5 TAHUN Eviwati Sitanggang; Bambang Dwirahardjo; Cahya Yustisia Hasan
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 2, Nomor 2, Desember 2015
Publisher : Universitas Baiturrahmah

Introduction: The Management of Anterior Maxillaris Dextra Fybrous Dysplasia at the 5 years old patient. Fibrous dysplasia is an abnormal bone growth where normal bone is replaced with fibrous connective tissue intermixed with irregular bony trabeculae. It causes bone pain, deformities & pathologic fracture. Fibrous dysplasia is a sporadic benign skeletal disorder that can affect one bone (monostotic) or multiple bone ( polyostotic). Disscusion: The clinical behavior and progression of fibrous dysplasia may also vary, thereby making the management of this condition difficult with few established clinical guidelines. Conclusion: This paper provides a biopsy and reconturing as the choice of treatment.

Effect of pre-operative 40 mg oral methylprednisolone on post- odontectomy facial swelling, intraoral redness, pain and level of TNF-α Harpindo Yusa; Cahya Yustisia Hasan; Bambang Dwirahardjo
Majalah Kedokteran Gigi Indonesia Vol 8, No 1 (2022): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.55116

Odontectomy is the surgical removal of teeth by making a mucoperiosteal flap and reducing the jawbone. This procedure is likely to cause injury and damage to soft and hard tissues, stimulate inflammatory responses, and generate release of proinflammatory cytokines, one of which is TNF- ɑ, resulting in the facial swelling, intraoral redness, and pain. This study was aimed at observing effects of 40 mg methylprednisolone, administered 1 hour before odontectomy on facial swelling, intraoral redness, and pain and level of TNF-ɑ after odontectomy. The randomized placebo-control trial study involved 24 subjects who underwent odontectomy at the Oral Surgery and Maxillofacial clinic of Prof. Soedomo Dental Hospital, Universitas Gadjah Mada. To comply with the inclusion criteria, the subjects were divided into placebo group (12 patients) and methylprednisolone group (12 patients). The observation of facial swelling, intraoral redness, pain (VAS) and level of TNF-ɑ (ELISA) was done before odontectormy, H+1 (24 hours after odontectomy) and H+3 (72 hours after odontectomy). The data gathered were analyzed using Repeated Measures ANOVA and post-hoc Bonferroni (p < 0.05). The results showed that those administered with methylprednisolone an hour before odontectomy experienced less postoperative facial swelling (p = 0.000), a lower score of intraoral redness (p = 0.000), a lower score of pain (p = 0.000) and a lower level of TNF-ɑ (p = 0.000) compared to the placebo. The changes in TNF-α showed the strongest correlation with the changes in postoperative pain and intraoral redness compared with facial swelling. Oral administration of 40 mg methylprednisolone an hour before odontectomy is more effective in reducing facial swelling, intraoral redness, pain and level of TNF-ɑ following odontectomy of mandibular third molar compared with the placebo.

Evaluation of Biodegradable Fixation Application for Jaw Fracture in Pediatric Patients Prasetya, Denta Aditya; Widiastuti, Maria Goreti; Hasan, Cahya Yustisia
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.284-291

Background: The treatment of jaw fractures in pediatric patients need a special concern and different management than adult patients due to their dynamic and structural changes following their growth patterns. Treatment by open reduction and internal fixation (ORIF) using titanium or biodegradable plates as osteosynthesis material is indicated for displaced jaw fracture. Biodegradable fixation could be used as an alternative choice, using plates and screw that will decompose along with bone healing. Case: Two pediatric patients brought to RSUP Dr. Sardjito, Yogyakarta with complaints of jawbone fracture. The first patient was a 7-years-old woman diagnosed with mandibular symphysis fracture and a left mandibular kondilus fracture, while the second patient was a 15-years-old woman diagnosed with malunion of left maxillary fracture. Fracture treatment was performed using biodegradable fixation. The first patient was repositioned according to the anatomical state and the second patient the maxillary refracture was done before repositioning. Patients were evaluated clinically for up to three months postoperatively and radiological imaging was performed. Evaluation results show good jaw bone union, no malocclusion, no tooth mobility and no paresthesia in both patients. Intraoral wound dehiscence with exposed plate was found in the second patient at the vestibule of the left uper canine until premolar region and can be resolved by removal of the plates under local anesthesiaConclusions:. Biodegradable as an ideal osteosynthesis material for jaw fracture in pediatric patients

Evaluation of Biodegradable Fixation Application for Jaw Fracture in Pediatric Patients Prasetya, Denta Aditya; Widiastuti, Maria Goreti; Hasan, Cahya Yustisia
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.284-291

Background: The treatment of jaw fractures in pediatric patients need a special concern and different management than adult patients due to their dynamic and structural changes following their growth patterns. Treatment by open reduction and internal fixation (ORIF) using titanium or biodegradable plates as osteosynthesis material is indicated for displaced jaw fracture. Biodegradable fixation could be used as an alternative choice, using plates and screw that will decompose along with bone healing. Case: Two pediatric patients brought to RSUP Dr. Sardjito, Yogyakarta with complaints of jawbone fracture. The first patient was a 7-years-old woman diagnosed with mandibular symphysis fracture and a left mandibular kondilus fracture, while the second patient was a 15-years-old woman diagnosed with malunion of left maxillary fracture. Fracture treatment was performed using biodegradable fixation. The first patient was repositioned according to the anatomical state and the second patient the maxillary refracture was done before repositioning. Patients were evaluated clinically for up to three months postoperatively and radiological imaging was performed. Evaluation results show good jaw bone union, no malocclusion, no tooth mobility and no paresthesia in both patients. Intraoral wound dehiscence with exposed plate was found in the second patient at the vestibule of the left uper canine until premolar region and can be resolved by removal of the plates under local anesthesiaConclusions:. Biodegradable as an ideal osteosynthesis material for jaw fracture in pediatric patients

Silkworm cocoon (Bombyx mori) accelerates wound healing in skin excision: a study on macrophage and VEGF Sudirman, Sudirman; Hasan, Cahya Yustisia; Arindra, Pingky Krisna
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.87352

Silkworm cocoon (Bombyx mori) is a natural polymer composite and largely used as bio-functional material for wound healing. It consists of fibroin and sericin protein that has antibacterial effect. This study aimed to investigate the effect of silkworm cocoons (Bombyx mori) wound dressing on the number of macrophages and VEGF expression in skin excision. The subject of this study was 12 Wistar rats, which were grouped based on the duration of wound dressing application (3rd day and 6th day) and the use of dressing materials (n = 6). The rats were anesthetized with ketamin and xylazine prior to wound excision. A punch biopsy wound excision with 4 mm diameter of subcutaneous depth was made on both sides of the rat’s back, with the right side dressed with silkworm cocoon as the treatment group and the left side dressed with moist gauze application as the control group. Hematoxylin-eosin (HE) staining was performed to observe the number of macrophage cells. Immunohistochemical staining using an anti-VEGF antibody was performed to observe the expression of VEGF. Data were analyzed using a two-way ANOVA and an Independent t-test with confidence interval of 95%. Statistical analysis demonstrated a significantly higher number of macrophages in the silkworm cocoon wound dressing group on 6 days post-application (p = 0.026) and significantly higher VEGF expression in the silkworm cocoon wound dressing group on 3 days post-application (p = 0.002) and on 6 days post-application (p = 0.044). Silkworm cocoon (Bombyx mori) wound dressing can increase the number of macrophages and VEGF expression in wound excision model in Wistar rat.

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