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I Nengah Kerta Besung

Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 5273870

Veterinary

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Identifikasi Staphylococcus sciuri dan S. hominis pada Ikan Kerapu di Pasar Ikan Kedonganan dengan Analisis Sekuen 16S rRNA I Nengah Kerta Besung; Ni Komang Eka Agustiani; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Grouper are reef fish which can be cultivated because highly commercial value. There are many different types of bacteria in the digestive tract of grouper, one of them is Staphylococcus. Staphylococcus sp. is a Gram positive, non spora, non motile, and facultative anaerobic. Most of Staphylococcus sp. are pathogenic in human and animals. The purpose of this study was to identify the species Staphylococcus sp. on grouper sold at the Kedonganan fish market, Kuta District, Badung Regency, Bali Province based on 16S rRNA sequence analysis. This study used grouper feces as a samples from 20 fishes. Staphylococcus sp. from feces were cultured in media Blood Agar. Sequence analysis 16S rRNA Staphylococcus sp. was conducted with PCR and sequencing method. This study can identify the bacteria Staphylococcus sciuri and Staphylococcus hominis.

Analisis Gen Patogenik iroN Escherichia coli Penyebab Kolibasilosis pada Ayam Kampung Tania Ria Gunawan; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.308 KB)

Avian colibacillosis is an infectious disease caused by avian pathogenic Escherichia coli (APEC). APEC ability to cause disease depends on many pathogenic factors, one of them is iroN pathogenic gene. This study purposed to find out iroN gene sequence in APEC in Bali. Two APEC isolates from free range chicken in Tabanan dan Badung has been used. The isolates have been been purified and were available at Laboratory of Veterinary Bacteriology, Faculty of Veterinary Medicine, Udayana University. Chelex 10%was used for DNA isolation. DNA amplification using published DNA primer has been conducted with polymerase chain reaction (PCR) method. The PCR product was sequenced at First Base Laboratories, Malaysia using Sanger’s Dideoxy Nucleotide Termination method. The iroN gene of both isolates can be analyzed and have 659 bp in length. Both were 100% homologous. Phylogenic test using 52 DNA sequence of iroN gene from Escherichia coli and other bacteria in the world was conducted in MEGA 5.2. All data have 24 polymorphic sites of nucleotide acid and eight polymorphic sites of amino acid. The iroN gene of Bali isolates was in the same group as iroN gene from Australia (MF174860) and Hong Kong (MF474175). This gene can be used as pathogenic marker of APEC in Indonesia.

An Amino Acids on Bali Cattle and Wagyu Beef Based on Different Function of Muscle (ASAM-ASAM AMINO SAPI BALI DAN DAGING SAPI WAGYU BERDASARKAN FUNGSI OTOT YANG BERBEDA) I Nengah Kerta Besung; Rasdianah Rasdianah; I Wayan Suardana; Ni Ketut Suwiti
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Beef is an essential source of protein and several functional compounds that are very important for human. The quality of beef depends on both genetic and environmental factors like feed, age, sex, and others. This research aimed to determine the composition of amino acids both Bali and Wagyu beef on the different activity of muscle, i.e. active and passive. As many as 5 g of each sample was used in this study. The active beef samples were presented by Biceps femoris, and passive beef samples were presented by Longissimus dorsi. High-Performance Liquid Chromatography (HPLC) method was used in order to an identification of amino acids according to the standard procedure. Results of the study showed that the essential amino acids content both bali cattle and wagyu were higher than non-essential, and amino acids content originated from active muscle was higher than passive muscle. Methionine, phenylalanine, and serine on bali beef cattle were lower than wagyu beef. Overall, the content of amino acids essential was lower than non-essential. In conclusion, there is no significant difference of amino acids content both bali cattle and wagyu beef, but the function of muscle (active or passive) were known contribute to the difference of amino acids content.

Pakan Tambahan dan Anabolik Growth Promoter Meningkatkan Kadar Hormon Pertumbuhan Sapi Bali Ni Made Riska Adnyani; Ni Ketut Suwiti; I Gusti Ayu Agung Suartini; I Nengah Kerta Besung
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aims to determine the level of growth hormone given feed supplement and anabolic growth promoter. This research was an experimental research using Complete Random Design (CRD) with three factors. There were feed supplement, anabolic growth promoter, and sampling time. There were 20 youngmale Bali cattle and they were taken care intensively, divided into 4 group, there were control, feed supplement, anabolic growth promoter, and the combination of feed supplement and anabolic growth promoter for five months, and measured the level of growth hormone each month. The level of growth hormone was detected by using competitive Enzyme Linked Immunosorbent Assay method. The result of the research showed that feed supplement and anabolic growth promoter increased the level of bali cattle growth hormone where feed supplement with anabolic growth promoter (P1G1) was not significantlydifferent than given feed supplement without anabolic growth promoter (P1G0). Providing feed supplement optimally by farmers is highly recommended.

Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS) Ni Ketut Suwiti; I Wayan Masa Tenaya; I Nengah Kerta Besung
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objective of this research was to compare the levels of growth hormone in bali cattle reared in the purification of Nusa Penida with those from other regions in Bali Province. A total of 320 sera samples were collected from bali cattle reared in Nusa Penida, Klungkung, Tabanan, Buleleng and Bangli. The levels of growth hormone was determined by using sandwich Enzime Linked Immunosorbent Assay (ELISA). The results showed that a diverse levels of growth hormone in Bali cattle were observed in Bali cattle. The lowest growth hormone levels (576.4 pg/mL) was found in Bali cattle reared in Nusa Penida and the highest levels (5044.08 pg/mL) were found in the Buleleng regency. It was concluded that bovine growth hormone levels varies, depending on the regions of rared of Bali cattle. ABSTRAK Penelitian ini bertujuan untuk mendeteksi kadar hormon pertumbuhan sapi bali yang dipelihara di wilayah pemurnian Nusa Penida dan wilayah lainnya di Provinsi Bali. Sebanyak 320 sampel serum sapi bali betina dewasa dikumpulkan dari peternakan rakyat di Nusa Penida Klungkung, Tabanan, Buleleng, dan Kabupaten Bangli. Kadar hormon pertumbuhan dideteksi dengan metode Enzime Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan, kadar hormon pertumbuhan sapi bali terendah (576,4 pg/mL) ditemukan pada sapi bali yang dipelihara di Nusa Penida, sedangkan kadar hormon tertinggi (5044,08 pg/mL) ditemukan pada sapi bali yang dipelihara di Kabupaten Buleleng. Disimpulkan bahwa kadar hormon pertumbuhan sapi bali berbeda-beda, tergantung wilayah tempat pemeliharaan sapi bali.

Sejumlah Faktor yang Melandasi Persepsi dan Perilaku Dokter Hewan terhadap Resistansi Antimikrob dan Penggunaan Antimikrob Vera Paulina Sitanggang; I Gede Hendra Prasetya Wicaksana; I Nengah Kerta Besung; Hapsari Mahatmi
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.424

Antimicrobial resistance (AMR) is the ability of bacteria to withstand the effects of drugs so that bacteria do not die after the administration of antimicrobials and the function of the drug does not work at all. This incident is caused by the uncontrolled use of antimicrobials which can cause a global crisis on human and animal health in the future. This study aims to determine the factors that influence the behavior and perceptions of veterinarians in the province of Bali on the use of antimicrobials and antimicrobial resistance. A total of 204 veterinarians were used as research samples through a survey using a Likert system and then the results of differences in veterinary responses to the frequency of prescribing were analyzed by Kruskal-Wallis. The results showed that antimicrobials were given to half of the patients treated weekly, and the factors that influenced the administration of antimicrobials were the history of antimicrobial use, level of safety, experience, potency, side effects, and the risk of increasing antimicrobial resistance. The cost of culture testing and the price of antimicrobials are the biggest barriers for small animal veterinarians but not for livestock veterinarians. All respondents acknowledged that most sources of information on AMR were easy to obtain and useful for them. The group of veterinarians who practice small animals and livestock practitioners agree that AMR is a serious common threat in the future. This study provides important insights into the application of veterinary medical procedures in the province of Bali and will later become a reference in increasing regulation control over the use of antimicrobials.

Co-Authors Dewa Putu Pradipta Baskara Fitri Indah Permatasari Hapsari Mahatmi I Gede Eka Chandrawan I Gede Hendra Prasetya Wicaksana I Gusti Agung Ayu Suartini I Gusti Ketut Suarjana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Wayan Masa Tenaya I Wayan Suardana Ida Bagus Ngurah Swacita Ike Siwi Widyaningtyas Ketut Tono Pasek Gelgel Made Tubagus Dharmajaya Ni Ketut Suwiti Ni Komang Eka Agustiani Ni Made Riska Adnyani Ni Putu Nicky Mirahsanti Ni Wayan Ayu Rukmini Nyoman Anandiya Ramaditya Pieter Mbolo Maranata Putu Henrywaesa Sudipa Rasdianah Rasdianah Sumanna Rumapea Tania Ria Gunawan Vera Paulina Sitanggang Vera Paulina Sitanggang

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