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All Journal Buletin Veteriner Udayana Jurnal Veteriner

I Nengah Kerta Besung

Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 5273870

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Perbandingan Jumlah Bakteri Coliform dan Non-Coliform yang Diisolasi dari Feses Ayam Petelur pada Berbagai Kelompok Umur Made Tubagus Dharmajaya; I Gusti Ketut Suarjana; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study was conducted to compare the number of coliform and non-coliform bacteria in laying feces on various age groups. This study used a Randomized Block Design (RBD) with four age treatments, namely the starter, grower, developer, and layer phases, respectively. Each treatment used six replications, with a total of 24 samples. The samples obtained were diluted with 10-5 dilutions and planted on Eosin Methylene Blue Agar (EMBA) media. After incubating for 18-24 hours at 37 ° C, the bacteria count was then calculated. Data on the number of coliform and non-coliform bacterial colonies grown on EMBA media were analyzed for variety and continued with BNT test when it was significantly different. The results showed that the number of coliform and non-coliform bacteria in laying hens in various age groups was not significantly different.

Analisis Marka Gen Patogenik hlyF pada Escherichia coli Penyebab Kolibasilosis pada Ayam Buras I Gede Eka Chandrawan; Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Colibacillosis by Avian Pathogenic Escherchia coli (APEC) is an infectious disease in poultry. The ability of APEC to cause disease depends on various virulence factors, including hlyF. The study aimed to prove the existence of the APEC hlyF gene in Bali and to find out the DNA sequences of these hlyF genes and their kinship with the hlyF gene from other countries. This study used two APEC isolates from free range chicken in Tabanan and Badung regencies. The isolates have been purified and were available at the Bacteriology Laboratory of Veterinary Medicine Faculty, Udayana University. DNA isolates were isolated using Chelex 10%. The hlyF gene is amplified using published DNA primers by polymerase chain reaction (PCR). PCR products were sequenced at First Base Laboratories in Malaysia using the Sanger’s Dideoxy Nucleotide Termination method. Both the hlyF gene sequences that can be analyzed have 100% homology with a readable length of 518 bp. Phylogenic test with 25 DNA sequences of hlyF gene on Escherichia coli and other bacteria in the world using UPGMA method with bootstrap (500 repetitions) was conducted with MEGA 5.2. All data have six polymorphic sites of nucleic acid and two polymorphic sites of amino acid . The hlyF gene from Bali was in same group with hlyF genes from various countries in the world. This gene can be used as a pathogenic marker of APEC in Indonesia.

Total Bakteri Feses Ayam Petelur pada Berbagai Periode Pemeliharaan Dewa Putu Pradipta Baskara; I Nengah Kerta Besung; Ketut Tono PG
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims to determine the total number of bacteria isolated from laying hens at various ages. The sample used was healthy laying hens' feces with 24 samples using the pouring method. This study uses a completely randomized design (CRD). The data obtained were tested with the Least Significant Difference Test (LSD). The results showed the total number of bacteria in the starter phase of layer was 52.83x107 CFU/ml, grower phase of layer was 208.50x107 CFU/ml, developer phase of layer was 409.5x107 CFU/ml, and layer phases of layer was 208x107 CFU/ml. It can be concluded that the total number of bacteria in the layer is significantly different at each age phase.

Deteksi Bakteri Berpotensi Patogen Pada Penyakit Saluran Pernapasan Komplek Babi I Gusti Ketut Suarjana; I Nengah Kerta Besung; Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims to detect bacterial infectious agents in pigs with complex respiratory tract disease known as porcine respiratory disease complex (PRDC) in some pig herds in Bali and look for the right medication is use to handle the incidence. The research sample comes from Tabanan, Badung and Gianyar totaling 48 Samples were isolated and identified by methods: isolation, the primary test, biochemical test. Test of sensitivity to antibiotics using disc diffusion method according to Kirby-Bauer method. The result showed that bacterial agents isolated from pigs were among others Streptococcus spp. alfa hemolytic non-coagulase (41,6%), Staphylococcus spp. beta hemolytic noncoagulase (41,6%) and Escherichia coli. Staphylococcus spp. beta hemolytic non-coagulase and E. coli were sensitive to streptomycin, kanamycin, doxicyclin, clindamycin and bacitracin. To conclude, Streptococcus spp. alfa hemolytic non-coagulase were sensitive to streptomycin, kanamycin, clindamycin and bacitracin, but resisten to doxicyclin.

Deteksi dan Sekuensing Gen iroN, iutA, dan hlyF pada Avian Pathogenic Eschericia coli Nyoman Anandiya Ramaditya; I Nengah Kerta Besung; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Research has been carried out to detect pathogenic gene markers of Avian Pathogenic Eschericia coli (APEC) iroN, iutA, and hlyF in Escherichia coli bacteria isolated from organs of sick chickens in Bali, and to determine phylogenetic relationships between those marker genes in Bali and in the other countries in the world. Six isolates of E. coli bacteria with codes E2, E3, E7, E8, E9, and E10 were used in this study. The isolates were isolated from domestic chicken in 2018. All genes were detected using the Polymerase Chain Reaction (PCR) method. The genes of iroN, iutA, and hlyF could be detected from all isolates. Well readable sequence of iroN, hlyF, and iutA was 659 bp, 518 bp, and 250 bp, respectively. All three genes were homogenous. Phylogical analysis shows that all pathogenic markers share same cluster with the pathogenic E. coli from all countries in the world.

Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Ayam Petelur I Nengah Kerta Besung; I Gusti Ketut Suarjana; I Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

The study aim was to determine the resistance of Escherichia coli to antibiotics that isolated in laying hens. A total of 100 samples of chicken feces were taken from Tabanan, Bangli and Karangasem districts. E. coli were isolated and identified by Carter (1990) method, followed by a bacterial susceptibility test with agar diffusion method from Kirby Bauer against antibiotics namely oxytetracycline (30 µg), ampicillin (10 µg), streptomycin (10 µg), sulfamethoxazole (30 µg), chloramphenicol (30 µg), and kanamycin (30 µg). The obtained data compared to the next sensitivity standard of the bacteria that classified as sensitive, intermediate, and resistant. The results showed that E. coli from chicken feces are sensitive to kanamycin, whereas the other antibiotics are intermediate or resistant. The increase in resistance is in line to the age of the chicken.

Identifikasi Staphylococcus sciuri dan S. hominis pada Ikan Kerapu di Pasar Ikan Kedonganan dengan Analisis Sekuen 16S rRNA I Nengah Kerta Besung; Ni Komang Eka Agustiani; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Grouper are reef fish which can be cultivated because highly commercial value. There are many different types of bacteria in the digestive tract of grouper, one of them is Staphylococcus. Staphylococcus sp. is a Gram positive, non spora, non motile, and facultative anaerobic. Most of Staphylococcus sp. are pathogenic in human and animals. The purpose of this study was to identify the species Staphylococcus sp. on grouper sold at the Kedonganan fish market, Kuta District, Badung Regency, Bali Province based on 16S rRNA sequence analysis. This study used grouper feces as a samples from 20 fishes. Staphylococcus sp. from feces were cultured in media Blood Agar. Sequence analysis 16S rRNA Staphylococcus sp. was conducted with PCR and sequencing method. This study can identify the bacteria Staphylococcus sciuri and Staphylococcus hominis.

Analisis Gen Patogenik iroN Escherichia coli Penyebab Kolibasilosis pada Ayam Kampung Tania Ria Gunawan; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian colibacillosis is an infectious disease caused by avian pathogenic Escherichia coli (APEC). APEC ability to cause disease depends on many pathogenic factors, one of them is iroN pathogenic gene. This study purposed to find out iroN gene sequence in APEC in Bali. Two APEC isolates from free range chicken in Tabanan dan Badung has been used. The isolates have been been purified and were available at Laboratory of Veterinary Bacteriology, Faculty of Veterinary Medicine, Udayana University. Chelex 10%was used for DNA isolation. DNA amplification using published DNA primer has been conducted with polymerase chain reaction (PCR) method. The PCR product was sequenced at First Base Laboratories, Malaysia using Sanger’s Dideoxy Nucleotide Termination method. The iroN gene of both isolates can be analyzed and have 659 bp in length. Both were 100% homologous. Phylogenic test using 52 DNA sequence of iroN gene from Escherichia coli and other bacteria in the world was conducted in MEGA 5.2. All data have 24 polymorphic sites of nucleotide acid and eight polymorphic sites of amino acid. The iroN gene of Bali isolates was in the same group as iroN gene from Australia (MF174860) and Hong Kong (MF474175). This gene can be used as pathogenic marker of APEC in Indonesia.

An Amino Acids on Bali Cattle and Wagyu Beef Based on Different Function of Muscle (ASAM-ASAM AMINO SAPI BALI DAN DAGING SAPI WAGYU BERDASARKAN FUNGSI OTOT YANG BERBEDA) I Nengah Kerta Besung; Rasdianah Rasdianah; I Wayan Suardana; Ni Ketut Suwiti
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Beef is an essential source of protein and several functional compounds that are very important for human. The quality of beef depends on both genetic and environmental factors like feed, age, sex, and others. This research aimed to determine the composition of amino acids both Bali and Wagyu beef on the different activity of muscle, i.e. active and passive. As many as 5 g of each sample was used in this study. The active beef samples were presented by Biceps femoris, and passive beef samples were presented by Longissimus dorsi. High-Performance Liquid Chromatography (HPLC) method was used in order to an identification of amino acids according to the standard procedure. Results of the study showed that the essential amino acids content both bali cattle and wagyu were higher than non-essential, and amino acids content originated from active muscle was higher than passive muscle. Methionine, phenylalanine, and serine on bali beef cattle were lower than wagyu beef. Overall, the content of amino acids essential was lower than non-essential. In conclusion, there is no significant difference of amino acids content both bali cattle and wagyu beef, but the function of muscle (active or passive) were known contribute to the difference of amino acids content.

Pakan Tambahan dan Anabolik Growth Promoter Meningkatkan Kadar Hormon Pertumbuhan Sapi Bali Ni Made Riska Adnyani; Ni Ketut Suwiti; I Gusti Ayu Agung Suartini; I Nengah Kerta Besung
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aims to determine the level of growth hormone given feed supplement and anabolic growth promoter. This research was an experimental research using Complete Random Design (CRD) with three factors. There were feed supplement, anabolic growth promoter, and sampling time. There were 20 youngmale Bali cattle and they were taken care intensively, divided into 4 group, there were control, feed supplement, anabolic growth promoter, and the combination of feed supplement and anabolic growth promoter for five months, and measured the level of growth hormone each month. The level of growth hormone was detected by using competitive Enzyme Linked Immunosorbent Assay method. The result of the research showed that feed supplement and anabolic growth promoter increased the level of bali cattle growth hormone where feed supplement with anabolic growth promoter (P1G1) was not significantlydifferent than given feed supplement without anabolic growth promoter (P1G0). Providing feed supplement optimally by farmers is highly recommended.

Co-Authors Dewa Putu Pradipta Baskara Fitri Indah Permatasari Hapsari Mahatmi I Gede Eka Chandrawan I Gede Hendra Prasetya Wicaksana I Gusti Agung Ayu Suartini I Gusti Ketut Suarjana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Wayan Masa Tenaya I Wayan Suardana Ida Bagus Ngurah Swacita Ike Siwi Widyaningtyas Ketut Tono Pasek Gelgel Made Tubagus Dharmajaya Ni Ketut Suwiti Ni Komang Eka Agustiani Ni Made Riska Adnyani Ni Putu Nicky Mirahsanti Ni Wayan Ayu Rukmini Nyoman Anandiya Ramaditya Pieter Mbolo Maranata Putu Henrywaesa Sudipa Rasdianah Rasdianah Sumanna Rumapea Tania Ria Gunawan Vera Paulina Sitanggang Vera Paulina Sitanggang

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