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All Journal BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jurnal Riset Biologi dan Aplikasinya Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Syubbanul Wathon, Syubbanul
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Physics Public Health

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PLATELET AGGREGATION IN VITRO ANALYSIS OF 67 kDa IMMUNOGENIC PROTEIN FRACTION FROM Aedes albopictus SALIVARY GLAND (SKUSE) (DIPTERA: CULICIDAE) Rike Oktarianti; Syubbanul - Wathon; Intan Fitri Indrasari; Nadya Rismana Fitriani; Kartika Senjarini
BIOEDUKASI Vol 18 No 2 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i2.18891

Abstract

Cardiovascular disease is caused by impaired heart and blood vessel function. Coronary heart disease includes acute coronary syndrome due to narrowing of the coronary arteries. Aspirin is an anti-platelet drug which is commonly used for primary and secondary prevention of coronary heart disease. The effectiveness of aspirin has limitations because 10-20% of patients who use aspirin continue to experience vascular blockage. The haematophagus arthropods salivary glands contain apyrase which can inhibit platelet aggregation and thus a potential candidate for anti-platelet drug discovery. Our studies from salivary gland protein extract of Aedes albopictus found a 67 kDa protein fraction which has a similar molecular weight range from the previously identified apyrase of other mosquitoes vectors. The purpose of this study was to determine the apyrase potential capacity of 67 kDa protein fraction from salivary gland of Ae. albopictus. The present study employed inhibition percentage of platelet aggregation method in determining its apyrase activity. Aspirin was used as a positive control with 2 different concentrations of 0.1 mg/mL and 2 mg/mL. PBS 1mM pH 7.4 was used as a negative control treatment, while negative control without treatment only involved the addition of PRP and ADP. The inhibition percentage activity from the 67 kDa sample ranged from 3-37% whereas the total protein extract comprised of only 1%. The positive control of aspirin was 0.1 mg/mL and 2 mg/mL, resulting in inhibition percentage of 5% and 17%, respectively. The data showed that the inhibition percentage of platelet aggregation from protein 67 kDa is generally higher than those of total salivary gland protein extract as well as positive control. This indicated that the 67 kDa protein has a potential apyrase activity.
KARAKTERISASI BERBASIS MARKA MOLEKULER ITS2 TERHADAP SUB-SPESIES KOMPLEKS Anopheles vagus vagus DAN Anopheles vagus limosus Kartika Senjarini; Lailly Nur Uswatul Hasanah; Miatin Alvin Septianasari; Muhammad Khalid Abdullah; Rike Oktarianti; Syubbanul Wathon
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.15 KB) | DOI: 10.29122/jbbi.v8i2.4737

Abstract

The presence of intraspecies variations of An. vagus later categorized as the subspecies of An. vagus vagus and An. vagus limosus, could be an obstacle to the identification process, which is an important step for malaria vector’s competence characterization. Based on morphological identification, those subspecies could be distinguished by the presences of pale scales in prehumeral and pale bands  in proboscis. The objective of this research was to compare subspecies complexes of An. vagus morphologically and molecularly using Internal Transcribed Spacer 2 (ITS2). Anopheles samples were collected from Bangsring, Banyuwangi. Their phylogenetic tree was constructed by using NJ method based on their ITS2 sequences. BLAST result showed that An. vagus vagus and An. vagus limosus were similar to An. vagus FJ654649.1 from East Java Indonesia and East Timor based on its 99% homology and their molecular distance. The Neighbour Joining (NJ) tree grouped those subspecies in one clade with a boostrap value of 82%. This subspeciation might be due to the different rates of evolution. ITS2 sequences of An. vagus vagus and An. vagus limosus were submitted to GenBank with the accession number of MW314227.1 and MW319822.1, respectively. Kemunculan variasi intraspesies An. vagus yang kemudian dikategorikan sebagai subspesies An. vagus vagus dan An. vagus limosus menjadi kendala dalam proses identifikasi yang merupakan langkah penting dalam menentukan kompetensi vektor malaria. Berdasarkan karakter morfologi, subspesies tersebut dibedakan dengan adanya sisik pucat pada bagian prehumeral dan pita pucat pada probosis. Penelitian ini bertujuan untuk membandingkan subspesies An. vagus secara morfologis dan molekuler menggunakan Internal Transcribed Spacer 2 (ITS2). Nyamuk Anopheles didapatkan dari Bangsring, Banyuwangi. Konstruksi pohon filogeni dilakukan berdasarkan sekuen ITS2 yang dianalisis menggunakan metode NJ. Hasil BLAST menunjukkan, ITS2 An. vagus vagus dan An. vagus limosus memiliki tingkat homologi 99% dan jarak evolusi molekuler terendah dengan An. vagus FJ654649.1 dari Jawa Timur Indonesia dan Timor Timur. Pohon NJ mengelompokkan subspesies tersebut dalam satu klade dengan nilai boostrap 82%. Hal ini dapat terjadi karena perbedaan kecepatan evolusi yang memungkinkan terjadinya subspesiasi. Urutan basa ITS2 dari An. vagus vagus dan An. vagus limosus telah didaftarkan ke GenBank dengan nomor aksesi MW314227.1 dan MW319822.1.
USING sma-its2 PRIMER FOR ITS2 (Internal Transcribed Spacer-2)-BASED MOLECULAR CHARACTERIZATION OF Anopheles minimus FROM KULONPROGO, YOGYAKARTA - INDONESIA Syubbanul Wathon; Berlian Permata Dewi Erlambang; Naura Paramitha Cindy Ardyah; Devi Astikaningrum; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.31911

Abstract

Malaria is a public health threat caused by the Plasmodium infection transmitted by the Anopheles mosquito. Malaria vector control is highly dependent on the ability to determine mosquito species' vectorial and bionomic capacity. Species identification based on morphological characteristics as well as DNA-barcoding approaches is a very important step in determining vectorial capacity. Our research group has redesigned a new ITS2 primer, namely, sma-its2, which is specifically able to identify the Anopheles (An.) mosquito vector but cannot identify other mosquito vectors. This study wanted to test this primer's specificity further for identification of other Anopheles mosquitoes. We used An. minimus collected from Kulonprogo, Yogyakarta – Indonesia. The methods used in this research are as follows: landing collection, morphological identification, isolation of genomic DNA, PCR (Polymerase Chain Reaction), PCR product purification, sequencing, and data analysis. An. vagus from Bangsring, Banyuwangi – Indonesia, which had previously been identified using the same primary, was used as a positive control. The results of the morphological analysis showed that both species were in accordance with the vector identification key used in this study. The molecular analysis showed that the sma-its2 primer could amplify the ITS2 sequence of An. vagus and An. minimus, producing 650 – 700 bp. However, further analysis of the ITS2 sequences of both species, resulted in the same species, namely An. vagus, with a different accession number in GenBank. This showed that the sma-its2 primar can be used to identify An. vagus but cannot be used to identify An. minimus. Analysis of the primer position in the ITS2 sequences showed the presence of 3 nucleotides in the forward sma-its2 primer that was not recognized by the An. minimus sequences and thus, hinder the successful identification of these species.
In silico study to predicting Apyrase-ADP binding affinity from salivary gland of Aedes aegypti in inhibition of platelet aggregation by molecular docking Rike Oktarianti; Silvya Fitri Nur Azizah; Ari Satia Nugraha; Syubbanul Wathon; Kartika Senjarini
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.33227

Abstract

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In silico exploration of the potential barcode DNA in Anopheles sp., a malarian vector from Indonesia Syubbanul Wathon; Devi Astikaningrum; Naura Paramitha Cindy Ardyah; Rike Oktarianti; Kartika Senjarini
Jurnal Biolokus Vol 6, No 1 (2023): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v6i1.1749

Abstract

Malaria is an infectious disease caused by Plasmodium and transmitted to humans through the Anopheles mosquito vector. The large diversity of Anopheles in Indonesia, which consists of similar complex species, makes it difficult to identify species based on morphological characteristics. Therefore, it is necessary to have another approach to identifying species based on molecular characteristics using DNA molecular markers. This approach becomes important as a basis for vector-based malaria control efforts. This research aims to explore potential DNA barcodes based on in silico studies as a reference for DNA barcoding analysis of Anopheles species in Indonesia. The data sequences of CO1 and ITS2 from Anopheles sp. were collected on the NCBI database. The sequence alignment was performed using CLUSTALX2. The construction of a phylogenetic tree was carried out using MEGA XI. Data analysis was performed by observing the construction of phylogenetic trees, bootstrap scores, and genetic distance. The data sequence in NCBI shows that 10 species of the genus Anopheles as malaria vectors have been studied in Indonesia. Sequence alignment of the 10 species based on CO1 and ITS2 markers showed that ITS2 markers had higher genetic variation than CO1. Therefore, the ITS2 sequence has the potential to be a DNA barcode for the molecular identification of Anopheles.
Relative Frequency (Composition) of Anopheles sp. Mosquitoes as Malaria Vectors in Kulon Progo District Special Region of Yogyakarta Wathon, Syubbanul; Senjarini, Kartika; Masruroh, Binti; Oktarianti, Rike; Rehmann, Holger
Jurnal Riset Biologi dan Aplikasinya Vol. 6 No. 1 (2024)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v6n1.p20-33

Abstract

Malaria is caused by Plasmodium infection transmitted Anopheles sp. One of the obstacles in malaria control is the variation of Anopheles sp. Species which have various characteristics and behaviour, so it’s very important to understand the species of Anopheles sp. with aim to develop the disease control program. This study was conducted in Kulon Progo Regency, Yogyakarta, one of the malaria endemic areas in Indonesia. Data collection of Anopheles sp. species diversity was carried out through landing collection inside and outside people’s homes at 18.00 - 06.00 WIB. Observations of abiotics factors in the habitat of Anopheles sp. larvae was carried out around the landing collection location. The result of research conducted in the Samigaluh, Kalibawang, and Pengasih Sub-Districts showed that the most common species found was Anopeheles vagus. The dominance of Anopheles vagus occurred in the Kalibawang and Pengasih dictricts, while in the Samigaluh Sub-Districts it became the second most among other species. Measurement of abiotic factors in the habitat of Anopheles sp. larvae through the parameters of temperature, air humidity, pH DO of water shows that the location where the larvae were found can support larval breeding and growth. That can be a factor in the large population of Anopheles sp., especially Anopheles vagus, which is a known vector of malarias based on research and its vectorial capacity.
Genetic Study of Phenylthiocarbamide (PTC) Taste Sensitivity In Population of The Osing in Kemiren Village-Banyuwangi Indriani, Fenny; Oktarianti, Rike; Wathon, Syubbanul
BERKALA SAINSTEK Vol 9 No 1 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i1.19844

Abstract

The ability to taste phenylthiocarbamide (PTC), is autosomal trait inherited in a simple Mendelian recessive pattern. The frequency of Taster and non-Taster allele is varies in different populations. The purpose of the research is to investigate the prevalence, gene frequency and genotype frequency of taster (T) and non taster (ts of Osing population in Kemiren-Banyuwangi. PTC serial dilution method was used to assess the PTC Taster and non-Taster phenotypes. The Hardy–Weinberg method was used to determine allele frequencies. The total of samples were 227 people, male were 117 and female were 110 with age range of 15–30 years were randomly selected. The result showed that the Osing population as Taster were 210 (92,52%) and non Taster were 17samples (7,48%) . The allele frecuency of Taster (T) was 0,73 and non Taster (t) was 0,27 respectively. The genotype frequency of dominant Taster (TT) was 0,54, heterozygosity Taster (Tt) was 0,39, and genotype of non Taster (tt) was 0,07.
Prevalensi Buta Warna Siswa Sekolah Menengah Atas di Kota Jember Oktarianti, Rike; Wathon, Syubbanul; Wulandari, Ayu Dwi
BERKALA SAINSTEK Vol 8 No 4 (2020)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v8i4.18127

Abstract

Buta warna merupakan kelainan genetis yang menyebabkan ketidakmampuan seseorang dalam membedakan warna seperti warna merah, biru, dan hijau. Buta warna merah-hijau merupakan salah satu tipe buta warna yang paling sering terjadi. Kelainan ini disebabkan oleh alel resesif c (color blind) yang terangkai pada kromosom X. Penelitian ini bertujuan untuk mengetahui prevalensi buta warna siswa SMA di Kota Jember. Penelitian ini dilaksanakan pada siswa kelas XI SMAN di Kota Jember yaitu SMAN 1, SMAN 2, SMAN 3, SMAN 4, dan SMAN 5 dengan pengambilan sampel secara acak sebanyak 353 siswa. Tes buta warna pada siswa menggunakan buku Ishihara. Analisis data dilakukan untuk mengetahui prevalensi buta warna, frekuensi alel, dan untuk menguji hukum kesetimbangan genetik Hardy-Weinberg menggunakan uji Chi-Square pada taraf signifikasi 0,05. Berdasarkan hasil penelitian ini diketahui bahwa prevalensi buta warna siswa SMA di Kota Jember adalah 3,68%, dengan frekuensi alel buta warna 0,09 dan alel normalnya adalah 0,91. Hasil pengujian kesetimbangan genetik Hardy-Weinberg menunjukkan nilai Chi-Square 1,74 pada derajat bebas = 3, dengan nilai probabilitas antara 0,80 dan 0,50 yang menujukkan tidak ada penyimpangan yang signifikan. Kesimpulannya adalah frekuensi genotip dan frekuensi alel buta warna pada siswa SMA di Kota Jember sesuai dengan hukum kesetimbangan genetik Hardy-Weinberg.
KARAKTERISASI PARSIAL FAKTOR IMUNOMODULATOR KELENJAR SALIVA Aedes aegypti (DIPTERA: CULICIDAE) SEBAGAI KANDIDAT Transmission Blocking Vaccine (TBV) DEMAM BERDARAH DENGUE Wathon, Syubbanul; Senjarini, Kartika; Widajati, Sri Mumpuni Wahyu; Oktarianti, Rike
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penyakit Demam Berdarah Dengue (DBD) disebabkan oleh infeksi virus dengue yang dibawa Aedes aegypti (Ae. aegypti) sebagai vektor primernya. Pengendalian vektor pada penyakit DBD masih belum maksimal. Selain itu vaksin yang belisensi untuk penyakit DBD masih belum dilaporkan. Melalui pengembangan TBV salah satunya dengan memanfaatkan komponen saliva vektor. aliva vektor memiliki potensi dalam meningkatkan transmisi patogen ke tubuh inang, maka perlu adanya karakterisasi molekul dalam saliva nyamuk termasuk faktor imunomodulator. Karakterisasi faktor imunomodulator saliva Ae. aegypti dilakukan melalui uji reaksi silang antara protein kelenjar saliva Ae. aegypti dengan beberapa plasma darah manusia. Hasil penelitian menunjukkan adanya protein spesifik yang dikenali antibodi dalam plasma darah orang endemik dengan berat molekul ~ 37 kDa. Hal ini megindikasikan bahwa di dalam tubuh penduduk endemik telah mengembangkan antibodi anti-saliva Ae. aegypti yang diduga berperan penting dalam resistensi terhadap infeksi virus dengue. Kata Kunci: Ae. aegypti, DBD, faktor imunomodulator, kelenjar saliva, TBV
Molecular characterization of proteolytic bacteria associated with Malaria vectors: Anopheles sundaicus and Anopheles vagus Senjarini, Kartika; Labes, Antje; Wathon, Syubbanul; Oktarianti, Rike; Raras, Tri Yudani Mardining; Ardyah, Naura Paramitha Cindy; Agustin, Dita Paramytha; Ainiyah, Durotun; Utami, Diah Ayu
Universa Medicina Vol. 43 No. 2 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.202-212

Abstract

BACKGROUNDAnopheles (An.) sp. transmits Plasmodium parasites that cause malaria. In its life cycle in the mosquito’s body, Plasmodium passes through 2 mosquito organs, namely the salivary glands and midgut. The bacterial community (symbiont bacteria) in these organs has been known to influence and/or inhibit the development of the Plasmodium life cycle by producing specific proteases. This research aims to isolate and characterize symbiotic bacteria with proteolytic activity from 2 important malaria vectors in Indonesia: An. sundaicus and An. vagus. METHODSA total of 183 bacterial originating from the salivary glands and midgut were successfully isolated. Initial screening was carried out based on morphological differences, followed by purification of the selected isolates to obtain single colonies. The selected isolates were then subjected to an initial proteolytic ability test using skim milk agar media. Only isolates with proteolytic activity were further characterized with the 16SrDNA molecular marker. The isolates were pabs5 from the salivary glands and pabs3 from the midgut of An. vagus, while pdbs3 and ecbs4 were isolates from the salivary glands and midgut of An. sundaicus. RESULTSMorphological and molecular characterization showed that both pabs5 and pabs3 isolates were Pseudomonas(Ps.) aeruginosa, while ecbs4 was Enterobacter cloacae and pdbs3 was Pantoea dispersa. These species werefirst discovered in Anopheles vagus and Anopheles sundaicus. CONCLUSIONThe ability of Ps. aeruginosa and Pantoea dispersa to produce proteases indicated their potential role in the exploration of new strategies to control mosquito vectors that transmit pathogens.
Co-Authors Agustin, Dita Paramytha Ahmad Tosin Ainiyah, Durotun Ardyah, Naura Paramitha Cindy Ari Satia Nugraha Berlian Permata Dewi Erlambang Devi Astikaningrum Devi Astikaningrum Eva Utami Fitria Mutiah Fitria Muti’ah Indriani, Fenny Intan Fitri Indrasari Kartika Senjarini Khasanah, Rochmatul Nuryu Labes, Antje Lailly Nur Uswatul Hasanah Lelono, Asmoro Mahri Ani MAsruroh, Binti Miatin Alvin Septianasari Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nuril Azizah Ratis Nour Sholichah Rehmann, Holger Riana Agatha Listiani Rike Oktarianti Silvya Fitri Nur Azizah Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Tri Yudani MR Utami, Diah Ayu Wulandari, Ayu Dwi Yasir Mubarok