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IN-SILICO ANALYSIS OF SYMBIONT BACTERIA DIVERSITY IN THE MIDGUT OF Aedes aegypti USING 16S rDNA MOLECULAR MARKERS DATABASE Syubbanul Wathon; Aufar Finasrullah; Rike Oktarianti; Kartika Senjarini
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Dengue Hemorrhagic Fever (DHF) is caused by the dengue virus, which is transmitted through Aedes aegypti mosquitoes when they feed on human blood. To effectively control the DHF vector, it is crucial to accurately characterize the symbiont bacteria associated with Ae. aegypti through an in-silico approach to identify potential targets. This study utilized in- silico analysis based on the 16S rDNA molecular marker to explore the diversity of symbiont bacteria obtained from bioinformatics databases. The analysis and visualization of bacterial diversity were conducted using the Pathosystem Resource Integration Center (PATRIC). The analysis results revealed that bacterial diversity in the midgut of Ae. aegypti, categorized as culturable and non-culturable bacteria, exhibited similar abundance patterns at the family level, albeit with varying detection rates. The most dominant taxa included the phylum Proteobacteria, class Gammaproteobacteria, order Enterobacterales, and family Enterobacteriaceae. Within the culturable bacteria category, the dominant taxa were the genus Salmonella and species Salmonella enterica, whereas the non-culturable bacteria category indicated the prevalence of the genus Escherichia and species Escherichia coli.

Characterization of marine bacteria isolates GaN 2 and RaN 2 from Papuma Coastal, Jember Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike; Hasanah, Fitrianti Milhatul; Oktavia, Melinda Dwi Putri; Puspitasari, Endah; Labes, Antje
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4289

The exploration of secondary metabolites for various human needs has now extended to aquatic environments. The fluctuating and dynamic nature of aquatic environments can stimulate marine bacteria to adapt by producing secondary metabolites. The diversity of marine bacteria has become increasingly promising as a target for obtaining various potential secondary metabolites. This study aims to isolate potential marine bacteria that produce secondary metabolites from the Papuma Beach area in Jember. Marine bacterial characterization can be carried out through morphological and molecular approaches. In this study, bacterial isolates were collected from marine algae at aquatic sites. Bacteria were isolated from the algae using the spread plate and streak plate methods on nutrient agar dissolved in artificial seawater as the medium until single colonies were obtained. Morphological characterization of the single colonies was conducted by observing colony shape and color, as well as performing Gram staining. Molecular characterization of selected single colonies was performed by amplifying the 16S rDNA as a bacterial molecular marker. The results of this study showed that marine bacteria that grew well and remained stable on artificial media were successfully isolated from green and red algae samples, labeled GaN 2 and RaN 2, respectively. The GaN 2 bacterial isolate was identified as Fictibacillus barbaricus strain SQ5-4, while the RaN 2 bacterial isolate was identified as Bacillus altitudinis strain 41KF2bT.26. According to previous studies, Fictibacillus barbaricus is known to have potential as a bioremediation agent, while Bacillus altitudinis has potential applications as an antitumor, antimicrobial, and antioxidant-producing agent.

IN-SILICO ANALYSIS OF THE INTERACTION BETWEEN D7 PROTEIN FROM THE SALIVARY GLAND OF Ae. albopictus AND Thromboxane A2 FOR DEVELOPING ANTIPLATELET AGENT Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike; Lelono, Asmoro
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8176

The salivary glands of mosquito vector diseases contain various biological components which facilitate blood-feeding into the host's body. These components are mostly protein molecules. Numerous protein molecules in the salivary glands have gained substantial research emphasis to determine their role and function, including those in the salivary glands of Ae. albopictus. D7 protein is the main component in Aedes salivary glands, which aids in inhibiting platelet aggregation by binding to the Thromboxane A2 (TxA2) during the blood-feeding. TxA2 is a eicosanoid molecule that stimulates platelet aggregation. The protein's ability to bind TxA2 shows that this protein has potential as a new antiplatelet agent. The examination of the D7 protein in binding TxA2 was performed through an in-silico approach using the molecular docking method. This research included selecting the 3D model of the D7 protein and the TxA2 ligand, preparing the 3D model of the D7 protein, native ligands, and test ligands, targeted molecular docking method, validating the molecular docking, analysis and visualization of the docking results. The molecular docking validation shows an RMSD value of 1.657 Å. The results of molecular docking show an ΔG value of -5.60 kcal/mol, meaning that the D7 protein can bind to the TxA2 ligand stably and spontaneously. The active site of the D7 protein in binding the TxA2 ligand consists of several amino acid residues, namely THR 190, GLU 268, TYR 178, PHE 154, ILE 175, ARG176, VAL 293, TYR 248, and TYR 178. The ability of D7 protein to bind TxA2 as an inducer of platelet aggregation has demonstrated its potential as a novel antiplatelet agent. These results can pave further development of drug discovery in the medical and pharmaceutical fields.

Characterization of Apyrase from the Salivary Glands of Ae. aegypti Based on Bioinformatics Database Wathon, Syubbanul -; Laili, Alvina Nur; Iflaha, Adriana; Widyadana, Kaysha Rahmadita; Zahniar, Zahniar; Saraswati, Dyah Rahmadhani; Oktarianti, Rike; Senjarini, Kartika
Life Science and Biotechnology Vol. 2 No. 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.48353

The salivary glands of mosquitoes, which act as disease vectors, contain biological components that facilitate the blood-feeding process and transmit pathogens to the human body. Apyrase, a protein from the salivary glands of Ae. aegypti, is known to be an immunogenic protein that influences the host's immunity. It can prevent platelet aggregation by hydrolyzing ATP or ADP into AMP and thus facilitate the blood-feeding process. This mechanism is also exploited by pathogens to obtain entry into the host's body. Vaccination with the apyrase protein could control dengue virus transmission by enhancing the immune response to inhibit the pathogen's entry. To examine the potential of apyrase, it is critical to explore its nature and characteristics by using bioinformatics databases. This quantitative descriptive study involved apyrase protein from the salivary glands of Ae. aegypti obtained from several bioinformatics databases, namely UniProt, SWISSMODEL, ENZYME, Rhea, CFSSP, GlyCosmos, STRING, and QuickGo. The findings contribute to a more fine-cut understanding of apyrase as a potential vaccine to inhibit dengue transmission.

IN-SILICO ANALYSIS OF SYMBIONT BACTERIA DIVERSITY IN THE MIDGUT OF Aedes aegypti USING 16S rDNA MOLECULAR MARKERS DATABASE Wathon, Syubbanul; Finasrullah, Aufar; Oktarianti, Rike; Senjarini, Kartika
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2841

Dengue Hemorrhagic Fever (DHF) is caused by the dengue virus, which is transmitted through Aedes aegypti mosquitoes when they feed on human blood. To effectively control the DHF vector, it is crucial to accurately characterize the symbiont bacteria associated with Ae. aegypti through an in-silico approach to identify potential targets. This study utilized in- silico analysis based on the 16S rDNA molecular marker to explore the diversity of symbiont bacteria obtained from bioinformatics databases. The analysis and visualization of bacterial diversity were conducted using the Pathosystem Resource Integration Center (PATRIC). The analysis results revealed that bacterial diversity in the midgut of Ae. aegypti, categorized as culturable and non-culturable bacteria, exhibited similar abundance patterns at the family level, albeit with varying detection rates. The most dominant taxa included the phylum Proteobacteria, class Gammaproteobacteria, order Enterobacterales, and family Enterobacteriaceae. Within the culturable bacteria category, the dominant taxa were the genus Salmonella and species Salmonella enterica, whereas the non-culturable bacteria category indicated the prevalence of the genus Escherichia and species Escherichia coli.

IN-SILICO ANALYSIS OF THE INTERACTION BETWEEN D7 PROTEIN FROM THE SALIVARY GLAND OF Ae. albopictus AND Thromboxane A2 FOR DEVELOPING ANTIPLATELET AGENT Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike; Lelono, Asmoro
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8176

The salivary glands of mosquito vector diseases contain various biological components which facilitate blood-feeding into the host's body. These components are mostly protein molecules. Numerous protein molecules in the salivary glands have gained substantial research emphasis to determine their role and function, including those in the salivary glands of Ae. albopictus. D7 protein is the main component in Aedes salivary glands, which aids in inhibiting platelet aggregation by binding to the Thromboxane A2 (TxA2) during the blood-feeding. TxA2 is a eicosanoid molecule that stimulates platelet aggregation. The protein's ability to bind TxA2 shows that this protein has potential as a new antiplatelet agent. The examination of the D7 protein in binding TxA2 was performed through an in-silico approach using the molecular docking method. This research included selecting the 3D model of the D7 protein and the TxA2 ligand, preparing the 3D model of the D7 protein, native ligands, and test ligands, targeted molecular docking method, validating the molecular docking, analysis and visualization of the docking results. The molecular docking validation shows an RMSD value of 1.657 Å. The results of molecular docking show an ΔG value of -5.60 kcal/mol, meaning that the D7 protein can bind to the TxA2 ligand stably and spontaneously. The active site of the D7 protein in binding the TxA2 ligand consists of several amino acid residues, namely THR 190, GLU 268, TYR 178, PHE 154, ILE 175, ARG176, VAL 293, TYR 248, and TYR 178. The ability of D7 protein to bind TxA2 as an inducer of platelet aggregation has demonstrated its potential as a novel antiplatelet agent. These results can pave further development of drug discovery in the medical and pharmaceutical fields.

PURIFIKASI FRAKSI PROTEIN IMUNOGENIK 47 kDa DARI KELENJAR SALIVA Aedes albopictus SEBAGAI TARGET PENGEMBANGAN VAKSIN DENGUE BERBASIS VEKTOR Wathon, Syubbanul; Rahmawati, Itsna; Oktarianti, Rike; Lelono, Asmoro; Senjarini, Kartika
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.810

Kapasitas vektorial Ae. albopictus telah diketahui sebagai vektor potensial virus dengue yang mengakibatkan DBD. Transmisi virus dengue terjadi ketika Ae. albopictus melakukan blood feeding ke manusia yang difasilitasi oleh aktivitas biologis protein pada kelenjar saliva vektor. Penelitian kami sebelumnya menunjukkan fraksi protein 47 kDa dari kelenjar saliva Ae. albopictus bersifat imunogenik. Protein 47 kDa merupakan serpins family yang diduga sebagai antikoagulan dan berperan sebagai protease inhibitor sehingga mempermudah transmisi virus dengue. Peran protein tersebut mengindikasikan adanya potensi dalam pengembangan vaksin penghambat transmisi patogen melalui vaksinasi melawan protein tersebut sehingga dapat menghambat transmisi dengue. Studi potensi protein tersebut memerlukan ekstrak murni, maka purifikasi protein target merupakan langkah yang sangat penting. Penelitian ini dimulai dengan landing collection dan rearing Ae. albopictus, isolasi dan ekstraksi protein kelenjar saliva, isolasi fraksi protein 47 kDa melalui SDS-PAGE, purifikasi protein, analisis dot blot dan western blot. Analisis SDS-PAGE menunjukkan pita tunggal protein target 47 kDa dari kelenjar saliva Ae. albopictus yang berhasil dipurifikasi menggunakan Electroeluter. Analisis dot blot menunjukkan protein 47 kDa terdeteksi bersifat imunogenik dan dikonfirmasi melalui analisis western blot bahwa protein target hasil purifikasi memiliki berat molekul 47 kDa.

PROFIL BIONOMIK VEKTOR POTENSIAL DENGUE DI KECAMATAN SUMBERSARI, KABUPATEN JEMBER Wathon, Syubbanul; Ali Maziun, Iffa; Oktarianti, Rike; Senjarini, Kartika
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.9215

Kabupaten Jember menempati urutan ketiga tertinggi kasus Demam Berdarah Dengue (DBD) di Jawa Timur, dengan angka tertinggi di Kecamatan Sumbersari. Beberapa upaya untuk memberantas kasus DBD telah dilakukan, misalnya pemberantasan sarang nyamuk (3M plus), fogging, penggunaan larvasida, “Gerakan Jumantik” namun masih ada kasus DBD khususnya di daerah endemik. Upaya penanggulangan DBD memerlukan studi bionomik untuk mencapai strategi yang efektif dalam program pengendalian vektor. Tujuan penelitian ini adalah untuk mengetahui bionomik vektor potensial DBD di wilayah endemik DBD Kecamatan Sumbersari, Jember, yang meliputi: identifikasi morfologi, proporsi jenis dan perilaku istirahat vektor nyamuk, serta karakteristik habitat dan kepadatan larva vektor nyamuk. Penelitian ini dilakukan dengan pendekatan metode purposive sampling pada beberapa lokasi di Kecamatan Sumbersari pada bulan September 2020. Koleksi sampel larva Aedes dilakukan dengan single larva methods sedangkan nyamuk dikoleksi dengan metode resting collection di dalam maupun luar rumah. Hasil penelitian menunjukkan bahwa proporsi Ae. aegypti (91%) lebih tinggi dibandingkan dengan Ae. albopictus (9%). Perilaku istirahat Ae. aegypti menunjukkan karakter endofilik, sedangkan Ae. albopictus lebih bersifat eksofilik. Puncak aktivitas istirahat Aedes spp. terjadi pada pukul 09.00–10.00 WIB. Karakteristik tempat perindukan Aedes spp. ditemukan di kontainer air berupa bak mandi (62%, dibandingkan jenis ember dan pot), berbahan semen (46%, dibandingkan bahan plastik dan keramik), berwarna gelap (77%, dibandingkan dengan berwarna terang) dan terletak di dalam ruangan (85%, dibandingkan dengan di luar ruangan). Kepadatan larva berdasarkan indek House Indicator di Kecamatan Sumbesari menunjukkan angka sebesar 30%. Berdasarkan tabel resiko penularan DBD, maka resiko penularan DBD pada Kecamatan Sumbersari termasuk dalam kategori tinggi.

Edukasi Kesehatan Pencegahan Demam Berdarah Dengue pada Siswa SDN 02 Panduman, Desa Jelbuk, Kabupaten Jember Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 3 (2025): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v10i3.6426

Dengue hemorrhagic fever (DHF) remains a major public health concern in tropical and subtropical countries, including Indonesia. School-aged children are among the most vulnerable groups due to limited awareness and daily habits that may support mosquito breeding. This community service activity aimed to enhance students’ knowledge of DHF prevention through health education at SDN 02 Panduman, Jelbuk Village, Jember Regency, East Java Province. Educational interventions were delivered interactively using PowerPoint presentations, posters, ovitrap demonstrations, videos, and pocketbooks adapted to the comprehension level of elementary school students. The evaluation was conducted using pre-test and post-test questionnaires consisting of 10 multiple-choice questions on dengue transmission, symptoms, and prevention. The results showed a significant improvement in student knowledge (25%), with average scores increasing from 63% to 88% (p < 0.001, Wilcoxon test). The distribution of illustrated pocketbooks served as an effective reinforcement tool, allowing students to share knowledge with their families and communities. Overall, the intervention successfully raised awareness and fostered preventive behavior among students, demonstrating that school-based health education is an effective strategy for reducing the risk of DHF transmission in endemic rural areas such as Jelbuk Village.

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