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All Journal Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Journal of Biomedicine and Translational Research Indonesian Journal of Biotechnology MNJ (Malang Neurology Journal) Universa Medicina
Tri Yudani MR
Laboratorium Biokimia - Biologi Molekuler Fakultas Kedokteran Universitas Brawijaya Malang
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Distribusi M. Tuberculosis Genotipe Beijing pada Pasien Tuberkulosis Paru di Malang MR, Tri Yudani; Astuti, Triwahju
Jurnal Kedokteran Brawijaya Vol 26, No 2 (2010)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.868 KB) | DOI: 10.21776/ub.jkb.2010.026.02.3

Abstract

ABSTRAKPenelusuran  penyebaran  genetis  Mycobacterium  tuberculosis  merupakan  salah  satu  faktor  penting  dalam mengendalikan infeksi Mycobacterium tuberculosis. Studi mengenai distribusi Mycobacterium tuberculosis galur Beijing pada TB paru BTA (+) pasien di RSU dr. Saiful Anwar rumah sakit ini   dilaksanakan dengan metode spoligotyping. Dahak dikumpulkan dari total 41 pasien, dicuci dengan salin normal dan ditanam pada medium LJ. DNA dari bakteri yang tumbuh kemudian diisolasi dan mengalami spoligotyping. Sembilan belas spoligotype (65,5%) ditemukan dari total dua puluh sembilan sampel yang berkembang, sedangkan sepuluh sampel (34,5%) adalah non-Mycobacterium tuberculosis. Dari spoligotype sembilan belas, sembilan sampel merupakan Beijing strain (31%) dan sepuluh sampel (34,5%) menunjukkan strain non Beijing. Proporsi pasien TB yang terinfeksi, menunjukkan bahwa persentase pasien terinfeksi dengan strain Beijing hampir sama antara wanita dan pria. Ditemukan bahwa prevalensi tertinggi strain Beijing pada pasien dengan usia berkisar antara 31-40 tahun (51%). Oleh karena itu dapat disimpulkan bahwa strain Beijing mendominasi distribusi M. tuberculosis pada pasien TB paru di Malang.Kata Kunci: Beijing strain, M. tuberculosis, Spoligotyping
Analysis Interaction of Immunoglobulin G and Immunoglobulin A Against PstS1 as a Basis Specimen Selection for M. tuberculosis Rapid Test Diagnostic Agent Rahmadani, Nabila -; Raras, Tri Yudani Mardining; Arthamin, Maimun Zulhaidah
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19431

Abstract

Background: The recombinant Ag38 protein developed from a local strain of Mycobacterium tuberculosis has great potential to be used as a sero-diagnosis agent using the antigen rapid test because it has several epitopes that bind to antibodies. However, it is not yet known which antibody Ag38-recombinant binds maximally between IgA and IgG.Objective: The aim of this study is to compare the interaction between IgG and IgA on Ag-38 rec in silico as a basis for the selection of sero-diagnosis agents in the TB rapid test.Methods: The results show that the protein PstS1 has a higher binding sensitivity to IgG based on one of the docking models which shows a docking score of -229.70, a confident score of 0.8312 and RMSD 1.060 A. The ramachandran plot also shows that testing on this model has a protein structure that is good, with disallowed regions [X,X] values of 0.5% (less than 0.8%). The results of this analysis show that the most favored regions are 90.5% with a G-factor of -0.27. The quality of the structure of the 3D mooring model can be said to be good because it fulfills the ideal structure requirements.Conclusion: Ag38-rec antigen M. tuberculosis H37Rv binds to IgG more strongly than IgA.
DOES THE COMBINATION OF ROSMARINUS OFFICINALIS AND CENTELLA ASIATICA NANOEMULSIONS AFFECT BDNF EXPRESSION IN THE GESTATIONAL DIABETES MELLITUS MODEL IN ZEBRAFISH LARVAE Khotimah, Husnul; Lamadi, Sutini; Amelia, Rizki; Arista, Devi Maya; Prastiwi, Fajar Dwi; Raras, Tri Yudani Mardining
MNJ (Malang Neurology Journal) Vol. 10 No. 2 (2024): July
Publisher : PERDOSSI (Perhimpunan Dokter Spesialis Saraf Indonesia Cabang Malang) - Indonesian Neurological Association Branch of Malang cooperated with Neurology Residency Program, Faculty of Medicine Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2024.010.02.08

Abstract

Background: Pancreatic β-cells compensate for the increased insulin demand due to increasing glucose levels during pregnancy or in gestational diabetes mellitus (GDM). The enzyme that regulates the rate of gluconeogenesis production is PEPCK. Circulatory and metabolic disturbances in the brain caused by hyperglycemia could affect brain-derived neurotrophic factor (BDNF) expression as an agent for neurodevelopment. Objective:  The purpose of this work is to ascertain the impact of combining nanoemulsions of Rosmarinus officinalis and Centella asiatica on the expression of BDNF and PEPCK. Methods: This study was a true experimental laboratory using zebrafish larvae. The larvae were exposed to 3% glucose and a combination of nanoemulsion extracts. The study sample of zebrafish larvae at 72 hpf (hour post fertilization) was divided into control, glucose, treatment 1, 2, and 3, with the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions at 2.5 μg/ml, 5 μg/ml, and 10 μg/ml, respectively. PEPCK and BDNF expression were measured by the real-time PCR method. Results: The expression of PEPCK increased significantly in the glucose group (3.05 ± 0.27). The combination of Rosmarinus officinalis and Centella asiatica nanoemulsions significantly decreased PEPCK (p-value 0,001 < 0.05). The BDNF level did not significantly differ (p-value 0.253 >0.05) among groups, but the highest level (1.06 ± 0.60) was gained in the T2 group, which received an embryonic medium (EM), 3% glucose, and the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions at 5μg/ml. Conclusion: The combination of Rosmarinus officinalis and Centella asiatica nanoemulsions significantly reduces PEPCK expression but does not affect BDNF expression.
Molecular characterization of proteolytic bacteria associated with Malaria vectors: Anopheles sundaicus and Anopheles vagus Senjarini, Kartika; Labes, Antje; Wathon, Syubbanul; Oktarianti, Rike; Raras, Tri Yudani Mardining; Ardyah, Naura Paramitha Cindy; Agustin, Dita Paramytha; Ainiyah, Durotun; Utami, Diah Ayu
Universa Medicina Vol. 43 No. 2 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.202-212

Abstract

BACKGROUNDAnopheles (An.) sp. transmits Plasmodium parasites that cause malaria. In its life cycle in the mosquito’s body, Plasmodium passes through 2 mosquito organs, namely the salivary glands and midgut. The bacterial community (symbiont bacteria) in these organs has been known to influence and/or inhibit the development of the Plasmodium life cycle by producing specific proteases. This research aims to isolate and characterize symbiotic bacteria with proteolytic activity from 2 important malaria vectors in Indonesia: An. sundaicus and An. vagus. METHODSA total of 183 bacterial originating from the salivary glands and midgut were successfully isolated. Initial screening was carried out based on morphological differences, followed by purification of the selected isolates to obtain single colonies. The selected isolates were then subjected to an initial proteolytic ability test using skim milk agar media. Only isolates with proteolytic activity were further characterized with the 16SrDNA molecular marker. The isolates were pabs5 from the salivary glands and pabs3 from the midgut of An. vagus, while pdbs3 and ecbs4 were isolates from the salivary glands and midgut of An. sundaicus. RESULTSMorphological and molecular characterization showed that both pabs5 and pabs3 isolates were Pseudomonas(Ps.) aeruginosa, while ecbs4 was Enterobacter cloacae and pdbs3 was Pantoea dispersa. These species werefirst discovered in Anopheles vagus and Anopheles sundaicus. CONCLUSIONThe ability of Ps. aeruginosa and Pantoea dispersa to produce proteases indicated their potential role in the exploration of new strategies to control mosquito vectors that transmit pathogens.
Overexpression and purification of YidRv gene from the hypervirulent Klebsiella pneumoniae, and the ability of the gene product in inducing a humoral response Raras, Tri Yudani Mardining; Ajrullah, Mauludy Jutta; Gunawan, Ellen Fenix; Pramesti, Nadya Shafa; Prawiro, Sumarno Reto; Wardani, Agustin Krisna; Helianti, Is
Indonesian Journal of Biotechnology Vol 29, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.95222

Abstract

The yidRv gene, isolated from the hypervirulent Klebsiella pneumoniae (hvKP), is a novel gene with an unknown function; however, it has exhibited high homology to the yidR, a gene recognized as potential vaccine candidate. The aim of this study was to clone the yidRv gene from the Indonesian hvKP and to investigate its overexpression in Escherichia coli. In the experiment, yidRv was cloned into pET21 to construct pYik23. Recombinant protein YidRv was produced by growing E. coli BL21 (DE3)/pYik23 in LB medium with ampicillin at 29 °C, inducing protein synthesis with 0.5 mM IPTG for 20 hours. Purification was performed using Ni‐NTA resin, and the purified protein (50 µg) was administered to BALB/c mice to test for the production of IgG, IgM and IgA on 2 days before and day 19th and 37th after the first vaccination. The results show a significant induction of IgG and IgM, but not of IgA antibodies. In conclusion, the yidRv gene was overexpressed in E. coli BL21 (DE3) at high levels in soluble form, with the recombinant protein able to be purified to 90% homogeneity. The recombinant YidRv demonstrated the ability to stimulate the generation of both IgM and IgG antibodies.
Exploring YidR Gene from Klebsiella pneumoniae To Design a Multi-epitope Vaccine Using Immunoinformatics Approach Wildan Seputra, Maulana; Susiati, Hani; Raras, Tri Yudani Mardining
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.08

Abstract

Klebsiella pneumoniae is the main leading cause of hospital-acquired infection, leading to severe infections such as pneumonia and urinary tract infection. There is an urge to develop an efficient Klebsiella vaccine, and epitope-based vaccines may offer a practical choice. Therefore, this study aims to design an epitope-based vaccine construct. YidR gene is expressed in all strains of Klebsiella with 97.6% of sequence homology, which makes it a potential vaccine candidate. In silico approach was used to make a multi-epitope vaccine construct consisting of immunogenic epitopes against the YidR gene of Klebsiella. Acquired multi-epitopes vaccine was analyzed for antigenicity, allergenicity, toxicity, and various physicochemical parameters. Molecular docking simulation was performed using TLR2 and TLR4 to asses binding affinity with the designed vaccine construct. The results showed that the predicted multi-epitope-based vaccine candidate is non-allergen, non-toxic, and has an efficient binding affinity towards TLR2 and TLR4 that could effectively induce immune responses. Keywords: In Silico, Klebsiella pneumoniae, vaccine design, YidR gene.
Co-Authors AA Sudharmawan, AA AGUSTIN KRISNA WARDANI Agustin, Dita Paramytha Ainiyah, Durotun Ajrullah, Mauludy Jutta Anandasari , Noni Fidya Ayu Anandasari, Noni Fidya Ayu Angelica, Aina Ardyah, Naura Paramitha Cindy Arista, Devi Maya Gunawan, Ellen Fenix Heni Pujiastuti IS HELIANTI Kartika Senjarini Kaspirayanthi, Ni Ketut Devy Kenty Wantri Anita, Kenty Wantri Kridayanti, Novalia Labes, Antje Lamadi, Sutini Maimun Zulhaidah Arthamin, Maimun Zulhaidah Pramesti, Nadya Shafa Prastiwi, Fajar Dwi Prawiro, Sumarno Reto Rahmadani, Nabila - Rike Oktarianti Rizki Amelia Subandi Subandi Susiati, Hani Syubbanul Wathon, Syubbanul Tatit Nurseta Triwahju Astuti Utami, Diah Ayu Wildan Seputra, Maulana Yudhanto, Hendy Setyo