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PHYTOCHEMICAL CHARACTERIZATION AND TANNIN STABILITY TEST FROM KLUWEK (Pangium edule Reinw) Siti Warnasih; Uswatun Hasanah
Journal of Science Innovare Vol 1, No 02 (2018): Journal of Science Innovare, September 2018
Publisher : Universitas Pakuan

One of the herbs that can be used as a source of natural dye is the kluwek which is brown. This study aims to determine the type of secondary metabolite compounds producing color, and to determine the stability of tannin in various conditions such as pH, oxidizing, UV rays, heating temperature, and storage using UV-Vis spectrophotometry method. This research includes preparation of kluwek, extraction with acetone:water (7:3) with maceration method, phytochemical test (tannin, flavonoid, carotenoid, and anthocyanin) and total tannins, and tannin stability test on various pH, oxidizing, UV rays, temperature and duration of heating, and storage using the UV-Vis spectrophotometry method. The results obtained are kluwek extract containing tannin, flavonoid, carotenoid, and anthocyanin compounds, and total tannins 2.80%. Based on the tannin content, kluwek extract stable at pH 6-7, heating 60-80 oC, and storage up to 9 days at temperature 27 C, and kluwek extract unstable when contact with 1% H2O2, UV light, and if kept at cold temperature (10 ?C).

DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING UV-VIS SPECTROPHOTOMETER Uswatun Hasanah; Ade Heri Mulyati; Sutanto .; Diana Widiastuti; Siti Warnasih; Yulian Syahputri; Tri Panji
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3007

The COD (Chemical Oxygen Demand) analysis method based on the Indonesian National Standard (SNI) is a revision of SNI 06-6989.2-2004, Water and wastewater - Part 2: Method of testing for chemical oxygen demand (COD) with closed reflux spectrophotometrically. This SNI uses references from international standard methods, namely Standard Methods for the Examination of Water and Wastewater, 21st Edition, editor of LS Clesceri, AE Greenberg, AD Eaton, APHA, AWWA and WEF, Washington DC, 2005, Methods 5220 D (Closed Reflux, Colorimetric Methods). The purpose of this study was to validate the total method for testing chemical oxygen demand (COD) in water and wastewater with the reduction of Cr2O7 2- spectrophotometrically in the range of COD values of 100 mg / L to 900 mg / L measurements were made at a wavelength of 600 nm. and COD values less than or equal to 90 mg / L measurements were made at a wavelength of 420 nm. The results showed that the method SNI 06-6898.2- 2009 has good validation results including the r results obtained from the calibration curve equation of 0.998, a precision of 1.82%, an accuracy of 98.25% and LOD and LOQ of 12.27 mg / L and 25, 61 mg / L.

DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING SPECTRODIRECT Ade Heri Mulyati; Leny Heliawati; Sutanto .; Uswatun Hasanah; Diana Widiastuti; Siti Warnasih; Yulian Syahputri
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3006

Increased human activities will cause various problems, one of which is water pollution in water sources because it receives a pollution load that exceeds its carrying capacity. In the context of environmental conservation, the government has set quality standards for liquid waste produced by various industries and other activities in a Decree of the State Minister for the Environment. COD (Chemical Oxygen Demand) is one of the most important parameters in wastewater quality standards. This study aims to validate the COD analysis method which refers to COD Thermoreactor System (LOVIBOND brand) at Pakuan University Service Laboratory, to ensure the validity of COD analysis results at Pakuan University Service Laboratory. Spectrodirect COD analysis method provides results that meet the requirements of accuracy, precision, LOD, and LOQ which is quite good. The results of the Coefficient of Variation give good results with a value of 1.8841% indicating the accuracy of the analysis method is good. The accuracy parameter also gives good results and meets the requirements with a% recovery value of 98.70351%. Overall, it can be stated that the spectrodirect analysis method tested is an accurate, precise, simple, and economical method for determining COD levels

POTENSI EKSTRAK CODIUM, HALIMEDA, DICTYOTA, CHONDRUS, GLACILLARIA SEBAGAI SUMBER PIGMEN DAN ANTIOKSIDAN ALAMI Tri Saptari Haryani; Uswatun Hasanah; Oom Komala; Intan Lestari; Dina Agustina
EKOLOGIA Vol 22, No 1 (2022): EKOLOGIA : JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v22i1.4739

Macroalgae is one of the marine biological resources that has an important role in terms of economy, as a source of food and medicine that is beneficial to health. This study aims to determine the source of natural pigments and antioxidants extracts of algae Codium, Halimeda, Dictyota, Chondrus, and Glacillaria using acetone, Folin Ciocalteu, and 1,1-Diphenyl-2-Picrylhydrazil (DPPH) reagents. The method includes sampling, measuring water content by gravimetry, making extracts using maceration method, qualitative phytochemical testing, pigment source test for chlorophyll a, chlorophyll b, total chlorophyll, total phenol, and antioxidant activity. Phytochemical test results obtained that all samples contained flavonoid compounds, alkaloids, while Halimeda and Gracillaria extracts did not have terpenoids. The results of the test for the pigment source were the highest value obtained in Chondrus extract, which was 10.0038 mg/g. And from the results of antioxidant activity testing, all sample extracts were obtained including the group with moderate strength (IC50 value) ranging from 100-500).

AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA Siti Warnasih; Diana Widiastuti; Uswatun Hasanah; Laksmi Ambarsari; Purwantiningsih Sugita
EKOLOGIA Vol 19, No 1 (2019): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

Micro-Scale Liquid Smoke Extraction as a By-Product from Coconut Shell Charcoal Production in Kotabatu Village Uswatun Hasanah; Ade Heri Mulyati; Lenny Heliawati
International Journal of Research in Community Services Vol 3, No 2 (2022)
Publisher : Research Collaboration Community (RCC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijrcs.v3i2.269

Liquid smoke is produced from the pyrolysis process and condensation from the coconut shell waste combustion process. This liquid smoke is known to have a high level of polyphenol content and is used as a rubber processing, deodorizing, and wood preservative to make it resistant to termites. In addition, it can also be used as an insecticide for plants. This community service is carried out to design and manufacture a micro-scale liquid smoke pyrolysis machine, which is expected to reduce the amount of smoke produced during the charcoal burning process, also to help partners create an additional type of product, namely liquid smoke. for plant insecticides so that it will increase partners' monthly income. The method that will be used in achieving this goal is to provide training and practice to partners on how to process cranium waste other than into charcoal, namely into liquid smoke as an insecticide in plants.

Chicken Bone Based Adsorbent for Adsorption of Pb(II), Cd(II), and Hg(II) Metals Ion Liquid Waste Uswatun Hasanah; Ani Iryani; Agus Taufiq; Dimas Apryandi Darma Putra
Helium: Journal of Science and Applied Chemistry Vol 1, No 1 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

High productivity of chicken meat for supplying society also make some impact in high productivity of chicken bone and because itâ€™s unraveling properties makes this become waste. Pharmaceuticalâ€™s Waste comes from production process, adstertion of production tools, laboratory activities and rejected product. Laboratoryâ€™s routine analysis also producing residual waste analysis, one of the source is come from heavy metals analysis of Pb, Cd, dan Hg. General process for waste treatment producing high quantity of sludge, mean while using third party service have impact in high costing of transportation and treatment process. An alternatif way for this case is using sorption treatment. Initial research starts from preparation of chicken bone-based adsorbent and its characterization using SEM and FTIR, followed by quality analysis of fresh waste (which is waste A and waste B) observed at pH, COD, TDS, TSS also Pb, Cd, dan Hg metals. Adsorption process was observed from adsorbentâ€™s graying temperature, adsorbentâ€™s particle size, contact time, pH of waste, and adsorbentâ€™s weight. Best result from adsorption process were applicated at fresh waste and simulation waste of Pb, Cd, and Hg metals also re-testing quality analysis of fresh waste and simulation waste for before-after result comparing purpose. The purpose of this research is for characterizing chicken bone-based adsorbent, observing adsorption process of Â Characterization of chicken bone ash based adsorbent has been carried out in adsorbing Pb, Cd, and Hg metals in pharmaceutical laboratory waste. Functional group analysis with Fourier Transform Infrared Spectrophotometer (FTIR) showed that the chicken bone ash adsorbent contained the mineral compound apatite carbonate. Surface morphology with a Scanning Electron Microscope (SEM) shows that the adsorbent surface is porous and appears to be adsorbing metals. From the adsorption observations, it was found that the best adsorption conditions were at an ashing temperature of 800 oC, particle size of 80 mesh, contact time of 30 minutes, waste conditions at pH 6 and an adsorbent weight of 600 mg. Application chicken bone-based adsorbent against pharmaceutical waste shows its existence improve the quality of waste but still do not meet the quality standard requirements for Hg metal.

DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING SPECTRODIRECT Ade Heri Mulyati; Leny Heliawati; Sutanto .; Uswatun Hasanah; Diana Widiastuti; Siti Warnasih; Yulian Syahputri
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare, Volume 03 Number 02 2020
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3006

Increased human activities will cause various problems, one of which is water pollution in water sources because it receives a pollution load that exceeds its carrying capacity. In the context of environmental conservation, the government has set quality standards for liquid waste produced by various industries and other activities in a Decree of the State Minister for the Environment. COD (Chemical Oxygen Demand) is one of the most important parameters in wastewater quality standards. This study aims to validate the COD analysis method which refers to COD Thermoreactor System (LOVIBOND brand) at Pakuan University Service Laboratory, to ensure the validity of COD analysis results at Pakuan University Service Laboratory. Spectrodirect COD analysis method provides results that meet the requirements of accuracy, precision, LOD, and LOQ which is quite good. The results of the Coefficient of Variation give good results with a value of 1.8841% indicating the accuracy of the analysis method is good. The accuracy parameter also gives good results and meets the requirements with a% recovery value of 98.70351%. Overall, it can be stated that the spectrodirect analysis method tested is an accurate, precise, simple, and economical method for determining COD levels

PHYTOCHEMICAL CHARACTERIZATION AND TANNIN STABILITY TEST FROM KLUWEK (Pangium edule Reinw) Siti Warnasih; Uswatun Hasanah
Journal of Science Innovare Vol 1, No 2 (2018): Journal of Science Innovare, Volume 01 Number 02 2018
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v1i02.1000

One of the herbs that can be used as a source of natural dye is the kluwek which is brown. This study aims to determine the type of secondary metabolite compounds producing color, and to determine the stability of tannin in various conditions such as pH, oxidizing, UV rays, heating temperature, and storage using UV-Vis spectrophotometry method. This research includes preparation of kluwek, extraction with acetone:water (7:3) with maceration method, phytochemical test (tannin, flavonoid, carotenoid, and anthocyanin) and total tannins, and tannin stability test on various pH, oxidizing, UV rays, temperature and duration of heating, and storage using the UV-Vis spectrophotometry method. The results obtained are kluwek extract containing tannin, flavonoid, carotenoid, and anthocyanin compounds, and total tannins 2.80%. Based on the tannin content, kluwek extract stable at pH 6-7, heating 60-80 oC, and storage up to 9 days at temperature 27 °C, and kluwek extract unstable when contact with 1% H2O2, UV light, and if kept at cold temperature (10 ˚C).

DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING UV-VIS SPECTROPHOTOMETER Uswatun Hasanah; Ade Heri Mulyati; Sutanto .; Diana Widiastuti; Siti Warnasih; Yulian Syahputri; Tri Panji
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare, Volume 03 Number 02 2020
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3007

The COD (Chemical Oxygen Demand) analysis method based on the Indonesian National Standard (SNI) is a revision of SNI 06-6989.2-2004, Water and wastewater - Part 2: Method of testing for chemical oxygen demand (COD) with closed reflux spectrophotometrically. This SNI uses references from international standard methods, namely Standard Methods for the Examination of Water and Wastewater, 21st Edition, editor of LS Clesceri, AE Greenberg, AD Eaton, APHA, AWWA and WEF, Washington DC, 2005, Methods 5220 D (Closed Reflux, Colorimetric Methods). The purpose of this study was to validate the total method for testing chemical oxygen demand (COD) in water and wastewater with the reduction of Cr2O7 2- spectrophotometrically in the range of COD values of 100 mg / L to 900 mg / L measurements were made at a wavelength of 600 nm. and COD values less than or equal to 90 mg / L measurements were made at a wavelength of 420 nm. The results showed that the method SNI 06-6898.2- 2009 has good validation results including the r results obtained from the calibration curve equation of 0.998, a precision of 1.82%, an accuracy of 98.25% and LOD and LOQ of 12.27 mg / L and 25, 61 mg / L.

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