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All Journal EduChemia: Jurnal Kimia dan Pendidikan JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Journal of Science Innovare EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup International Journal of Research in Community Services Helium: Journal of Science and Applied Chemistry
Uswatun Hasanah
Universitas Pakuan
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Other

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POTENSI EKSTRAK CODIUM, HALIMEDA, DICTYOTA, CHONDRUS, GLACILLARIA SEBAGAI SUMBER PIGMEN DAN ANTIOKSIDAN ALAMI Tri Saptari Haryani; Uswatun Hasanah; Oom Komala; Intan Lestari; Dina Agustina
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 22, No 1 (2022): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v22i1.4739

Abstract

Macroalgae is one of the marine biological resources that has an important role in terms of economy, as a source of food and medicine that is beneficial to health. This study aims to determine the source of natural pigments and antioxidants extracts of algae Codium, Halimeda, Dictyota, Chondrus, and Glacillaria using acetone, Folin Ciocalteu, and 1,1-Diphenyl-2-Picrylhydrazil (DPPH) reagents. The method includes sampling, measuring water content by gravimetry, making extracts using maceration method, qualitative phytochemical testing, pigment source test for chlorophyll a, chlorophyll b, total chlorophyll, total phenol, and antioxidant activity. Phytochemical test results obtained that all samples contained flavonoid compounds, alkaloids, while Halimeda and Gracillaria extracts did not have terpenoids. The results of the test for the pigment source were the highest value obtained in Chondrus extract, which was 10.0038 mg/g. And from the results of antioxidant activity testing, all sample extracts were obtained including the group with moderate strength (IC50 value) ranging from 100-500).
AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA Siti Warnasih; Diana Widiastuti; Uswatun Hasanah; Laksmi Ambarsari; Purwantiningsih Sugita
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 19, No 1 (2019): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v19i1.1660

Abstract

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.
Chicken Bone Based Adsorbent for Adsorption of Pb(II), Cd(II), and Hg(II) Metals Ion Liquid Waste Uswatun Hasanah; Ani Iryani; Agus Taufiq; Dimas Apryandi Darma Putra
Helium: Journal of Science and Applied Chemistry Vol 1, No 1 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v1i1.2949

Abstract

High productivity of chicken meat for supplying society also make some impact in high productivity of chicken bone and because its unraveling properties makes this become waste. Pharmaceuticals Waste comes from production process, adstertion of production tools, laboratory activities and rejected product. Laboratorys routine analysis also producing residual waste analysis, one of the source is come from heavy metals analysis of Pb, Cd, dan Hg. General process for waste treatment producing high quantity of sludge, mean while using third party service have impact in high costing of transportation and treatment process. An alternatif way for this case is using sorption treatment. Initial research starts from preparation of chicken bone-based adsorbent and its characterization using SEM and FTIR, followed by quality analysis of fresh waste (which is waste A and waste B) observed at pH, COD, TDS, TSS also Pb, Cd, dan Hg metals. Adsorption process was observed from adsorbents graying temperature, adsorbents particle size, contact time, pH of waste, and adsorbents weight. Best result from adsorption process were applicated at fresh waste and simulation waste of Pb, Cd, and Hg metals also re-testing quality analysis of fresh waste and simulation waste for before-after result comparing purpose. The purpose of this research is for characterizing chicken bone-based adsorbent, observing adsorption process of  Characterization of chicken bone ash based adsorbent has been carried out in adsorbing Pb, Cd, and Hg metals in pharmaceutical laboratory waste. Functional group analysis with Fourier Transform Infrared Spectrophotometer (FTIR) showed that the chicken bone ash adsorbent contained the mineral compound apatite carbonate. Surface morphology with a Scanning Electron Microscope (SEM) shows that the adsorbent surface is porous and appears to be adsorbing metals. From the adsorption observations, it was found that the best adsorption conditions were at an ashing temperature of 800 oC, particle size of 80 mesh, contact time of 30 minutes, waste conditions at pH 6 and an adsorbent weight of 600 mg. Application chicken bone-based adsorbent against pharmaceutical waste shows its existence improve the quality of waste but still do not meet the quality standard requirements for Hg metal.
Comparison of COD Determination Methods FAS Titrimetric with UV-Vis Spectrophotometry Intan Wulandari; Uswatun Hasanah; Siti Warnasih
Helium: Journal of Science and Applied Chemistry Vol 3, No 1 (2023): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v3i1.8097

Abstract

Liquid waste is one of the factors causing contamination of the aquatic environment. One of the chemical parameters of water quality namely Chemical Oxygen Demand (COD). This research was conducted at three different concentration levels, namely low, medium, and high, with samples of river water, domestic wastewater, and sago liquid waste. The method used is UV-Vis spectrophotometry (SNI 6989.2: 2019) and FAS titrimetry (APHA, 2017 methods 5220 D); for the UV-Vis spectrophotometry method in the range 90 mg/L, the wavelength was measured at 420 nm, while for high levels in the range 100 mg/L x 900 mg/L, it was measured at 600 nm. The quality control parameters used are accuracy and precision parameters. The purpose of this study was to compare the COD determination between UV-vis spectrophotometry and FAS titrimetry and to determine the validity and correlation of the two methods—a comparison of the results of the two methods used in the F test. The results showed that the COD values from UV visible spectrophotometry and FAS titrimetry yielded good precision and accuracy values and met the acceptable limits, namely %RSD 10% and 90% accuracy %R 110%. However, the results of the COD analysis using the UV-Vis spectrophotometry method were lower by 0.8556 than the results of the COD analysis using the FAS titration method, with a correlation coefficient r2 = 0.982. The average concentration of UV-Vis spectrophotometry in samples of sago wastewater was 572.141 mg/L, domestic wastewater was 113.525 mg/L, and river water was 42.98 mg/L. The average COD level of the titrimetric method in sago wastewater was 641.888 mg/L, domestic wastewater was 219.251 mg/L, and river water was 58.016 mg/L. The results of the F test for these two methods produce an Fcount Ftable. The null hypothesis (Ho) is rejected, meaning there is a significant difference between the two methods.
Identification and Antibacterial Activity of Bioactive Compounds Extracted from Gading Coconut (Cocos nucifera var. eburnea) Leaf Hasanah, Uswatun; Mulyati, Ade Heri; Riyadi, Sandra Amalia; Pita, Lusia Magdalenis
EduChemia: Jurnal Kimia dan Pendidikan Vol 10, No 1 (2025)
Publisher : Department of Chemistr Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/educhemia.v10i1.31974

Abstract

Traditional medicine continues to play a vital role in Indonesia’s healthcare landscape, with increasing reliance on plant-derived remedies for disease prevention and treatment. Among these, the gading coconut (Cocos nucifera var. eburnea), native to Bogor, Indonesia, presents untapped potential as a source of antibacterial agents. This study aimed to identify bioactive compounds with antibacterial activity from gading coconut leaves and to evaluate the impact of extraction methods—maceration and sonication—on their yield and efficacy. Compared with maceration (5.92%), sonication produced a significantly greater extract yield (9.48%). Phytochemical screening confirmed the presence of alkaloids, flavonoids, and tannins in both extracts. Quantitative analysis revealed that the sonicated extract contained greater concentrations of total flavonoids (52.06 mg QE/g) and total phenolics (147.10 mg GAE/g). Chemical profiling using liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) identified key antibacterial constituents, including 3-hydroxycoumarin, isoorientin, vitexin, and α-linolenic acid. Biological evaluation demonstrated that the sonicated extract exhibited stronger antibacterial activity, with minimum inhibitory concentrations (MICs) of 50 mg/mL against Escherichia coli and 25 mg/mL against Staphylococcus aureus. These findings suggest that the extraction method significantly influences both the chemical composition and antibacterial potency of gading coconut leaf extracts. Overall, this study highlights the pharmacological relevance of C. nucifera var. eburnea as a promising source of natural antibacterial agents and contributes to the scientific validation of traditional medicinal plants in Indonesia.
In-Vitro and In-Silico Study: The Anti-Inflammatory Activity of Ethanol Extract from Cogon Grass Roots (Imperata cylindrica L. Siti Warnasih; Uswatun Hasanah; Siska Juliani Simalango; Ade Heri Mulyati; Diana Widiastuti
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 12 No. 2 (2025): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v12i22025.264-276

Abstract

Background: Inflammation is a protective reaction triggered by harmful substances, microbes, or physical trauma. Non-steroidal anti-inflammatory drugs (NSAIDs) are commonly used to treat inflammation, though they have certain drawbacks, such as the potential for chronic kidney failure and unfavorable gastrointestinal side effects. Therefore, alternative treatments are needed. Cogon grass (Imperata cylindrica L.) roots contain secondary metabolites that may offer potential for inflammation treatment. Objective: This study aims to investigate the potential of secondary metabolites from cogon grass roots as anti-inflammatory agents, both in vitro using protein denaturation inhibition techniques and in silico against the COX-1 and COX-2 enzyme receptors. Methods: Molecular docking of COX-1 (PDB ID 6Y3C) and COX-2 (PDB ID 1PXX) using AutoDock Tool 1.5.6 was used to test the anti-inflammatory activity. In parallel, the in vitro technique involved spectrophotometric denaturation inhibition of the BSA (bovine serum albumin) protein. Results: The in silico results showed that the cyclovalone ligand exhibited the highest interaction and stability, with Gibbs free energies of -9.3 kcal/mol against COX-1 and -9.8 kcal/mol against COX-2, compared to the control ligand diclofenac, which had Gibbs free energies of -6.5 kcal/mol against COX-1 and -8.5 kcal/mol against COX-2. The 30% ethanol extract of cogon grass roots demonstrated anti-inflammatory activity in the in vitro analysis, with an IC50 value of 71.79 µg/mL. Conclusions: These preliminary findings suggest that the ethanol extract of cogon grass roots contains cyclovalone compounds with potential as anti-inflammatory agents.
Co-Authors Ade Heri Mulyati Ade Heri Mulyati Agus Taufiq Diana Widiastuti Diana Widiastuti Dimas Apryandi Darma Putra Dina Agustina Intan Lestari Intan Wulandari Iryani, Ani LAKSMI AMBARSARI Leny Heliawati Mulyati, Ade Heri Oom Komala Pita, Lusia Magdalenis Purwantiningsih Sugita Sandra Amalia Riyadi Siska Juliani Simalango Siti Warnasih Sutanto . Tri Panji Tri Saptari Haryani Yulian Syahputri