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Journal : Agrivet

PATHOGENICITY TEST OF VARIOUS CONCENTRATIONS OF Beauveria bassiana CULTURED ON RICE BRAN AND CORN MEDIA AGAINST COFFEE FRUITS BORER (Hyphotenemus hampei) Chimayatus Solichah; Anindita Widyaningtyas; Rukmowati Brotodjojo
Jurnal Agrivet Vol 25, No 2 (2019): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v25i2.4301

Abstract

Coffee is a commodity with high value for export. Coffee bean borer (Hypothenemus hampei) is the main pest causing the decline in coffee production. Farmers still rely on chemical insecticides to control this pest. To reduce the negative impact of insecticides, entomopathogen Beauveria bassiana is applied. The research aimed to investigating the level of pathogenicity of B. bassiana in controlling H. hampei, and investigating the concentration with the highest level of pathogenicity of B. Bassiana against H. hampei. Laboratory research was arranged in one factor Completely Random Design (CRD). This research used 8 (eight) treatments namely KO: Aquades, Kk: Lamda Sihalothrin 25 EC (Chemical Insecticide), B1: 10 g / L B. bassiana cultivated in rice bran media, B2: 20 g / L B. bassiana cultivated in rice bran media, B3: 30 g / L B. bassiana cultivated in rice bran media, B4: 10 g / L B. bassiana grown on corn media, B5: 20 gL B. bassiana grown on corn and B6: 30 g / L B. bassiana grown on corn media. Each treatment used coffee beans infested with 20 H. hampei and repeated 4 (four) times. The result of the research showed that B. bassiana was able to kill H. hampei. The concentration that was able to cause the fastest mortality with a high level of pathogenicity based on the pathogenicity test of B. bassiana on H. hampei (percentage of mortality, total mortality time, and feeding capacity) was 30 g/L of B. bassiana cultivated in rice bran media.Key words: Beauveria bassiana, Hypothenemus hampei, chemical insecticide, coffee bean
Efektivitas ekstrak biji nimba untuk pengendalian ulat kobis (Plutella xylostella) Rukmowati Brotodjojo
Jurnal Agrivet Vol 20, No 1 (2014): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v20i1.4642

Abstract

Synthetic insecticides have detrimental effect to environment. Botanical insecticides are considered to be safer to the environment. Need seeds contain compounds with insecticidal properties. The aim of this study was to examine the effectiveness of neem seeds extract to control Diamondback moth (DBM). A series of neem seeds extract concentration (0.04 %, 0.08 %, 0.16 %, 0.32 %) and control (without neem extract) were tested on second instar larvae in oral test and contact test. The results showed that neem seeds extract have significant effect on the mortality of second instar DBM larvae 24-72 h after treatment in oral test and 72 h after treatment in contact test. In the oral test, the neem seeds extract significantly reduced feeding intensity of the larvae 42 h and 72 h after treatment. Subsequently, the extract also significantly reduced the moth emergence. The neem seeds with concentration 0.16 % effectively control DBM second instar larvae 72 h after treatment.Key Words: neem seeds extract, diamondback moth, effectiveness
EFEKTIFITAS Trichoderma harzianum YANG DIPERBANYAK PADA BERBAGAI MEDIA DAN WAKTU APLIKASI BERBEDA UNTUK PENGENDALIAN Colletotrichum spp. PADA BUAH CABAI Endah Paramita Pratiwi; R.R. Rukmowati Brotodjojo; Chimayatus Solichah
Jurnal Agrivet Vol 28, No 2 (2022): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v28i2.7727

Abstract

Cabai merupakan salah satu komoditas yang bernilai ekonomi tinggi di Indonesia. Budidaya cabai dihadapkan pada penyakit tanaman yang salah satunya yaitu penyakit antraknosa yang disebabkan oleh Colletotrichum spp. Upaya pengendalian dengan Trichoderma harzianum diharapkan mampu menekan serangan patogen Colletotrichum spp. Penelitian ini bertujuan untuk mengetahui media T. harzianum yang terbaik dan waktu aplikasi yang tepat dalam mengendalikan Colletotrichum spp. Penelitian dilaksanakan di Laboratorium Proteksi Tanaman UPN “Veteran” Yogyakarta pada bulan Januari–April 2022. Rancangan yang digunakan yaitu Rancangan Acak Lengkap (RAL). Pada penelitian in-vitro digunakan 4 perlakuan media yaitu dedak, jagung, air kelapa, dan limbah cair tempe serta 1 perlakuan kontrol Colletotrichum spp. tanpa agen hayati.  T. harzianum dari media perbanyakan yang efektif pada uji in-vitro, dilakukan uji lanjut in-vivo  dengan perlakuan kontrol (tanpa fungisida dan agen hayati), Fungisida Propineb, T. harzianum sebelum inokulasi patogen, dan T. harzianum setelah inokulasi patogen. Parameter yang diamati pada uji in-vitro adalah persentase penghambatan (%) dan pada uji in-vivo adalah masa inkubasi (hari), kejadian penyakit (%), intensitas penyakit (%), dan AUDPC. Data yang diperoleh dianalisis dengan uji F ANOVA apabila hasil menunjukkan perlakuan berbeda  nyata kemudian dilakukan uji lanjut menggunakan Uji Jarak Berganda Duncan taraf 5%. Trichoderma harzianum yang diperbanyak pada media dedak efektif menekan patogen Colletotrichum spp. pada uji in-vitro, serta waktu aplikasi T. harzianum sebelum inokulasi patogen sama efektifnya dengan aplikasi T. harzianum setelah inokulasi patogen.