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All Journal Natural Science: Journal of Science and Technology Biocelebes Jurnal Kedokteran Universitas Palangka Raya
Orryani Lambui
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam UniversitasTadulako
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Medicine & Pharmacology Public Health

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UJI DAYA HAMBAT EKSTRAK BATANG Harrisonia perforata (Blanco) MERR. TERHADAP PERTUMBUHAN BAKTERI Streptococcus mutans Sa’adia Alydrus; Orryani Lambui; Ramadhanil Pitopang
Biocelebes Vol. 10 No. 1 (2016)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research about inhibition test of stem extract of Harrisonia perforata (Blanco) Merr. to the growth of Streptococcus mutans had conducted diving period of February to March 2015. The aim of this research was to study the inhibition test from stem extract plant of H. perforata (Blanco) Merr. on the bactery studied. The extraction was used reflux method.The experiment was arrenged in Completely Randomized Designed (CRD) with 5 treatments and 3 repititions. The treatments were stem extract concentrates 40%, 50% and 60%, positive control Tetrasiklin hidroklorida 2% and negative control Na-CMC 1%. The result showed that stem extract of H. perforata (Blanco) Merr. had inhibition effect to the growth of bacteria. The extract concentrations 60% produced the biggest inhibition zone for S. mutans 21,4 mm than other concentrations.   Keyword :  Stem extract of Harrisonia perforata (Blanco) Merr., Streptococcus mutans.
UJI DAYA HAMBAT EKSTRAK DAUN TUMBUHAN Harrisonia perforata (BLANCO) MERR. TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus Fitri Maija; Orryani Lambui; Ramadhanil Pitopang
Biocelebes Vol. 10 No. 1 (2016)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Research of inhibition test from the leaves extract of Harrisonia perforata (Blanco) Merr. on the growth of Staphylococcus aureus was carried out in February and March 2015. This study was aimed to determine the effectiveness of the leaves extracts from H. perforata (Blanco) Merr. for inhibiting the bacterial growth. The extraction used maceration methods and the inhibition assay from the extracts for S. aureus used wells diffusion method. The treatment was arranged in Completely Randomized Design (CRD) with 6 treatments and 3 replications. Concentration for this treatment leaves extract were 10%, 25%, 40%, and 65%, amoxicillin 2% as a positive control and NA-CMC 1% as a negative control. The results showed that the concentration leaves extract of 65% formed the highest in inhibition zone, which was 19,3 mm. It means that the leaves extract of H. perforata (Blanco) Merr. was better to inhibit the bacterial growth.   Keywords : Leaves extract, Harrisonia perforata (Blanco) Merr., Staphylococcus aureus.
Formulasi Substrat Dasar Kotoran Sapi dan Limbah Cair Tempe dengan menggunakan Inokulum Rumen Sapi Untuk Studi Awal Produksi Biogas Bidayanti Ruru; Umrah Umrah; Orryani Lambui
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research of “Biogas Production by Utilizing of Basic Substrate of  Fermented Liquid waste of tempeh and Cow Waste by Using Inoculum from Cow Rumen” for the basic study of biogas production was held on May until July 2016 at department Biology Laboratory unit Biotechnology, Faculty of Mathematic and Natural Sciences, University of Tadulako. The purposed of this research is to produce biogas by utilizing the basic of liguid waste of tempeh and cow waste, and also to know the concentration of using the rumen inoculum of cow rumen. This research is designed with completely randomized design which consists of six treatment with three repeating. the basic substance are composed of 500 grams of cow dung and 500 ml of liquid waste tempe.The composition of treatment is : ( P0 100% Basic Substrate Without inoculum),  ( P1 80% Basic Substrate + 20% inoculums) ,(P2 60% Basic Substrate + 40% inoculums), (P3 40% Basic Substrate + 60% inoculums) , (P3 20% Basic Substrate + 80% inoculums), (P5 100% inoculum Without basic substrate). Observation variable are consist of biogas volume measuring, pH measuring, light test and temperature measuring. anaerobic fermentation process is carried out in a culture bottle with a volume of 1000 ml bottle mouth top covered with a balloon for gas storage. The results of this research show that Biogas could be produced by using the basic substrate of fermented liguid waste of tempeh and cow waste with the inoculum from cow rumen, at, P2, P3, dan P4 treatment. The highest volume of gas in P2 treatment (60% Basic Substrate : 40% inoculum) which has 553,33 cm3. The pH which in the culture is about 6,33-6,46 and the result flame with temperature in 152ºC.
STUDI ETNOBOTANI TUMBUHAN OBAT PADA MASYARAKAT SUKU PAMONA DI DESA BUYUMPONDOLI, KECAMATAN PAMONA PUSELEMBA, KABUPATEN POSO, SULAWESI TENGAH Kurniawan P. Bandjolu; Orryani Lambui; Ramadanil Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The objectives of this study was to determine value of plants for Pamona community and to identify plant species that used traditionally as medicine. The method was survey method by using direct interview to thirteen (13) respondents. The number of respondents was determined by purposive and snow ball technique sampling. We recorded that there were thirty two (32) species of plant that used by Pamona community as medicine. Based on plant organ, the Pamona community used fruit 3 species, leaf 22 species, stem 4 species, rhizome 2 species and fruit skin 1 species.
UJI DAYA HAMBAT EKSTRAK DAUN SIRIH HUTAN (Piper aduncum L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Escherichia coli Is Patuh Hallianah; Orryani Lambui; Ramadanil Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Research about the inhibition test of leaf extract of Piper aduncum L.  on the growth of Staphylococcus aureus and Escherichia coli have been conducted during period of July to December 2016. The aim of this research was to study the effectiveness of leaf extract Piper aduncum L. in inhibition  of S. aureus and E. coli. The extraction method used in the study was method maceration, meanwhile bioassay of extract on the bacteria. by disc diffusion method. The research was design by Completely Randomize Design (CDR) with 5 treatments and 3 replications. The treatments were leaf extract concentration 10%, 30% and 60%.  Positive control Amoxicillin 3% and negative control aquadest. The result showed that leaf extract of Piper aduncum L. had  inhibition effect to the growth of bacterias. The extract concentrate 60% produced the biggest inhibition zone for both S. aureus and E. coli 14 mm. Phytochemical analyses has showed that leaf extract of Piper aduncum L. contained compounds flavonoids, tannins, saponins and alkaloids.UJI DAYA HAMBAT EKSTRAK DAUN SIRIH HUTAN (Piper aduncum L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Escherichia coli
Uji Daya Hambat Ekstrak Kulit Batang Kelor (Moringa oleifera Lamk.) Terhadap Pertumbuhan Bakteri Vibrio cholerae Nur Afiyah Labambe; Orryani Lambui; Ramadanil Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research about the inhibition test of bark extract of Moringa oleifera Lamk. to the growth of bactery Vibrio cholerae. It has been conducted on July until December. The aim of this esearch was to determine the effectiveness of bark extract of M. oleifera Lamk. to the growth of V. cholera bacteria and the content of bark extract M. oleifera Lamk. The extraction method used in this research was maceration testing method toward V. cholerae bacteria by using disc diffusion method. This research designed in a completely randomized design (CRD) with 6 treatments and 3 repetitions. At this research using stem bark extract concentration of 20%, 40%, 60% and 80%, the positive control using tetracycline hydrochloride 3% and a negative control using distilled water. The results showed that the concentration of bark extract 80% produced the greatest inhibition zone is 23.8 mm. This shows that the extract of the bark of Moringa oleifera Lamk. can inhibit Vibrio cholerae bactery.
Uji Daya Hambat Ekstrak Daun Sirih Hutan (Piper aduncum L.) Terhadap Pertumbuhan Bakteri Streptococcus mutans Nunung Safriana; Orryani Lambui; Ramadanil Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research about inhibition test of leaf extract of Piper aduncum L. to the growth of bacteria Streptococcus mutans have been conducted during periods of July to December 2016, on aim of this research was to study the inhibition test from leaf extract of P. aduncum L. the growth of the bacteria S. mutans and the amount of compound contained in the leaf of P. aduncum L. The extraction method  was used is maseration method and testing of the inhibition of the extract to the bacteria S. mutans by disc diffusion method. This research is compiled in a completely randomized designed (CRD) with 6 treatments and 3 replications. The treatments were leaf extract concentration 30%, 45%, 60%, 75%, antibiotic Tetracycline hydrochloride 5% as the positive control and negatif control aquades. The results showed that the concentrations  of leaf extract 75% produced the greatest inhibition zone is 13,1 mm. this indicates tha the leaf extract of P. aduncum L. have inhibitory better.  Phytochemical screening results showed that there were compound flavonoid, tannin, saponin and alkaloid that can inhibit the growth of bacteria.
UJI DAYA HAMBAT EKSTRAK DAUN BANDOTAN (Ageratum conyzoides L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus Melsi Mengkido; Orryani Lambui; Wahyu Harso
Biocelebes Vol. 13 No. 2 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Ageratum conyzoides L. is commonly known as weed species however leaf from this plant is used as traditional medicine for wound and skin infection. Furthermore it is important to test A. conyzoides L. leaves extract can inhibit the growth of Staphylococcus aureus bacteria which is causing infection disease. The content of this leaf which can inhibit bacterial growth is also obeserved. The study was conducted by growing S. aureus bacteria on nutrient agar (NA) and then a number of leave extract from A. conyzoides leaves (7.5, 15.0, 35 and 50%) were injected to NA by well diffusion method. Amoxicillin 0,6% and DMSO 1% were also injected as positve control and negative control respectively. Inhibition zone was measured based on diameter formed. The results showed that increasing leaves extract increased inhibition of S. aureus growth however 50% leave extract inhibited S. aureus growth less than Amoxicilin 0,6%.A. conyzoides leaf can inhibit S. aureus growth may be caused by terpenoid, fenol, saponin and alkaloid on its content.
UJI DAYA HAMBAT EKSTRAK DAUN LALUMPA (Melastoma malabathricum L.) TERHADAP PERTUMBUHAN BAKTERI Vibrio cholerae dan Staphylococcus aureus Mutmainnah Mutmainnah; Wahyu Harso; Orryani Lambui
Biocelebes Vol. 13 No. 2 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Vibrio cholera and Staphylococcus aureus are a gram negative and a gram positive bacteria respectively. Both of them can cause diseaces in human. They have differences in their cell wall composition. Differences in both bacteria in the resistance to antibacterial compounds interesting to learn. The aim of this study was to observe inhibition of Melastoma malabathricum leave extract to the growth of V. Cholera and S. aureus. The study was conducted with Completely Randomized Design. The treatment was tested with 10%, 20%, 40%, 60%, 80% of leave extract concentration. Amoxicillin 2% and Na-CMC 1% were also treated as positif and negative control. Each treatments was repeated three times. Extract was obtained by maceration method. Extract was injected on bacterial growth medium by well diffusion method. The result showed that increasing extract concentration increased inhibition of growth to both bacteria. V. cholera tended to be more resistant than S. aureus.
The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum Nisfaun Safitriana; Umrah; Orryani Lambui
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.187 KB) | DOI: 10.22487/bioceb.v13i3.14971

Abstract

The study about the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.
Co-Authors Abi Gael Sidon Alvionita Alvionita Alwi Millang Andi Aldi Matoro Putra Ardiputra, Muh.Akbar Bidayanti Ruru Cindy Claudia, Cindy Fitri Maija Handayani, Mutia I Nengah Suwastika Iis Salihat Imelda, Silvia Is Patuh Hallianah Khadijah Kurniawan P. Bandjolu LANGKAH SEMBIRING Magfirahtul Jannah Marina Marina Melsi Mengkido Musjaya M. Guli Mutmainnah Mutmainnah Nisfaun Safitriana Nunung Safriana Nur Afiyah Labambe Priyandini, Nabila Ramadanil Pitopang Ramadhanil pitopang Rosidah, Maya Sofiyanti S. Rahayu, S. Sa’adia Alydrus Talantan, Vrillia Marannu Toemon , Agnes Immanuela Umrah Umrah Umrah Umrah Wahyu Harso Widiawati Yunus, Fahrunnisa