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All Journal Jurnal Ilmu Lingkungan Bioscience Biota Jurnal Pendidikan Biologi Indonesia Edu Sains: Jurnal Pendidikan Sains dan Matematika Nusantara Science and Technology Proceedings These proceedings represent the work of researchers participating in The International Conference on Elementary Education (ICEE) which is being hosted by the Elementary Education Study Programme School of Postgraduate Studies, Universitas Pendidikan BiosciED: Journal of Biological Science and Education Journal of Multidisciplinary Applied Natural Science Journal of Peat Science and Innovation (JPSI)
Chaidir Adam
Biology Education Program, University of Palangka Raya, Palangka Raya – 74874 (Indonesia); Center for Development of Science, Technology and Peatland Innovation (PPIIG), University of Palangka Raya, Palangka Raya – 74874 (Indonesia)
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Journal : Bioscience

 1 
Analysis of Genetic Profiles of Heavy Metal Phytoremediator Plants From Gold Mining Areas Sih Winarti; Liswara Neneng; Yohanes Edy Gunawan; Chaidir Adam
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.732 KB) | DOI: 10.24036/0202041108305-0-00

Abstract

The resistance and survival ability of phytoremediator plants in a polluted environment is thought to be a form of genetic adaptation. This research aimed: (1) to identify the dominant plant species in ex-gold mining area; (2) to analyze the genetic profile of phytoremediator plants from ex-gold mining area; and (3) to compare the genetic profile of heavy metal phytoremediator plants from ex-gold mining area with the same plant species from non-mining area. The samples of this study were Cyperus sp., Lycopodium sp., and Melastoma sp. The research procedures carried out include sample collection, DNA isolation, DNA amplification with PCR, and DNA visualization with Electrophoresis. The results show that the dominant plant species of ex-gold mining area are Cyperus sp., Lycopodium sp., and Melastoma sp. The genetic profile analysis of dominant plant species of ex-gold mining area show that no DNA bands appeared from the target gene as the result of amplification using specific primers of Metallothionein gene. The result of RAPD analysis using OPA-04 universal primers show that at 500-750 bp there are differences in DNA bands that appeared between the samples. DNA bands that appeared in the genetic profile of phytoremediator plants is thought to be the representation of the gene that responsible for heavy metals tolerance.
Anti-Microbial Activities of Shallots (Allium cepa L.) Extract and Garlic (Allium sativum L.) Extract on the Growth of Peat Soil Bacteria Chaidir Adam
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151110068-0-00

Abstract

This study aims to examine the antimicrobial activity of red onion (Allium cepa L.) and garlic (Allium sativum L.) extracts on the growth of peat soil bacteria. This type of research is an experimental study using a Completely Randomized Design with four levels of treatment. Test for bacterial activity using the agar diffusion method with the paper disc technique. Data were analyzed statistically using One Way Anova and the Games-Howell post-hoc test at the 5% significance level. The results showed that red onion (Allium cepa L.) and garlic (Allium sativum L.) extracts had antimicrobial activity against the growth of tested bacteria as indicated by the presence of a clear zone as an indicator of inhibition of bacterial growth. Garlic has better antimicrobial activity seen from the diameter of the clear zone that appears in the garlic extract treatment ranging from 8-13 mm, whereas in the treatment of onions it ranges from 3–5 mm. 
Co-Authors AGUS HARYONO Agus Haryono Agus Haryono Agus Haryono Agus Haryono Araina, Elga Ardianoor Ardianoor Arintiana Ghazella Basuki, Bejo Elga Araina Fahrina, Ririn Gunawan, Yohanes E Indah Fitriani Liswara Neneng Ririn Fahrina Shanty Savitri Sih Winarti Sitti Minah Soaloon Sinaga Srininta Br Perangin-angin Sugeng Mashabhi Usup, Hepryandi Luwyk Djanas Vivin Alvianita Zakaria Zakaria