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LD50 ACUTE TOXICITY TEST OF GREEN GRASS JELLY (Cyclea barbata Miers) LEAF EXTRACT AGAINST MICE (Mus musculus L.): UJI TOKSISITAS AKUT LD50 EKSTRAK DAUN CINCAU HIJAU (Cyclea barbata Miers) TERHADAP MENCIT (Mus musculus L.) Hendri Poernomo; Taha Ma'aruf; Ari Sucitra Dewi
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 19 No. 1 (2023): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v19i1.6544

Introduction: Green grass jelly leaves (Cyclea barbata Miers) are well known by the public as food to reduce fever (fever), nausea, stomach ulcers, coughs and reduce high blood pressure, antioxidants, anti-inflammatories, and as analgesics, so safety needs to be controlled. Acute Toxicity Test is a test used to detect the toxic effect of a substance on a biological system and to obtain typical response data from test preparations, Lethal Dose 50 and toxicity classification. Research Objectives: To determine the safety of green grass jelly leaf extract (Cyclea barbata Miers) against mice (Mus musculus L.) by LD50 acute toxicity test. Research Methods: In the acute toxicity test using the Thomson-Weil calculation method and mice as experimental animals as many as 25 mice (Mus musculus). The mice were divided into 5 treatment groups with the main test series group I distilled water as the control, group II was given the test extract at a dose of 200 mg/kg, group III was given the test extract at a dose of 400 mg/kg, group IV was given the test extract at a dose of 800 mg/kg, and Group V was given the test extract at a dose of 1600 mg/kgBW with one oral administration and observed the number of deaths in each test animal after 24 hours to 14 days. Research Results: The results of the acute toxicity test showed that the LD50 value of green grass jelly leaf extract was 0.6166 gr/kg BW (moderately toxic). For acute toxicity, the weights of mice were analyzed using the ANOVA and LSD tests and for allergy testing using the Wilcoxon signed ranks test. Conclusion: the results of the analysis show that there is a significant difference at the test level of 0.05 (???? < 0.05).

Osteomyelitis therapy with hyperbaric oxygen: Terapi osteomielitis dengan oksigen hiperbarik Hendri Poernomo
Makassar Dental Journal Vol. 11 No. 3 (2022): Volume 11 Issue 3 Desember 2022
Publisher : Makassar Dental Journal PDGI Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35856/mdj.v11i3.654

Osteomyelitis is a diffuse inflammation that affects the periosteum, cortical bone, and cancellous bone components. The inside of the bone that has been infected will become soft and experience swelling which causes the tissue to press against the outer wall of the bone and the blood vessels in the marrow whichcauses blood flow and oxygen supply to the bone decrease drastically. Usually surgical therapy is needed to treat osteomyelitis, but if the patient's physical condition does not allow it, hyperbaric oxy-gen therapy can be done to increase the supply of oxygen to the hypoxic bones. Hyperbaric oxygen therapy is an adjunctto conven-tional antibiotic or surgical treatment of resistant osteomyelitis, using large, multiposition or monoposition pressure tubes for one patient. The patients are given 100% oxygen with a pressure dose of 2-2.4 Atm for 90-120 minutes per day; five or six times per week for 8 weeks or more. Overall usage ranges from 80 to over 130 hours.

ACUTE TOXICITY TEST OF AMBON BANANA TREE SAP EXTRACT ON MICE (Mus Muculus L.) Setiawan; Hendri Poernomo; Reza Pramana
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 19 No. 2 (2023): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v19i2.7480

Introduction: Banana tree sap is commonly used by the community as herbal medicine in the process of accelerating wound healing so it needs to be tested for the safety of its use. The research entitled Test of Acute Toxicity and Allergy of Ambon Banana Tree Sap Extract to Mice (Mus Muculus L.) aims to determine Lethal Dose 50, toxicity classification and to observe allergic symptoms dermally of Ambon banana tree sap extract to mice. Materials and Methods: In the acute toxicity test using the Thomson Weil calculation method using mice as experimental animals as many as 25 which were divided into 5 treatment groups with the main test series group I as the control group with 0.3% Na-CMC, groups 2-5 were given the test extract with doses of 300, 600, 1200 and 2400mg/kgBW with one oral administration and observed the number of deaths in each test animal for 14 days. Results and Discussions: In allergy testing using the Wilocoxon method. From the results of the acute toxicity test study, the LD50 value of banana tree sap extract was 1116.8mg/kgBW (1.116g/kgBW) included in the moderate toxic category (0.5-5g/kgBW) and in the allergy test it did not cause allergies (no rash and red-red). For acute toxicity, the weights of mice were analyzed using the ANOVA and LSD tests as well as the allergy test using Wilocoxon. The results of the analysis show that there is a significant difference at the test level of 0.05 (???? <0.05). in the allergy test obtained statistical test results which stated that there was no allergic reaction in mice during treatment with a ???? value of 1.000 > 0.05. Conclusion: Giving banana tree sap extract at certain doses can cause death in mice but does not cause any allergic symptoms.

Subacute Toxicity Test of Cocoa Bean Extract (Theobroma cacao L.) on Mice (Mus musculus L.) Hendri Poernomo; Setiawan
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 20 No. 2 (2024): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v20i2.9105

Introduction: Cocoa is one of the plants that is believed to have many health benefits and is used in herbal medicine so it is necessary to test the safety of its use. The purpose of the study was to determine abnormalities in the hepatic histopathology of mice that occurred after repeated administration of the test preparation. Materials and Methods: The research used in vivo laboratory experiments with the post-test only control group approach. The samples used were 25 consisting of 5 groups, namely the group of mice given cocoa bean extract at a dose of 4500 mg/kgBB, 5500 mg/kgBB, 6500 mg/kgBB, 7500 mg/kgBB, and the control group was given distilled water, and for the allergy test was given 32% cocoa bean extract gel. Oral administration of the test preparation was carried out every day for 28 days, once a day. Observations included toxic symptoms by looking at hepatic organ histopathology. Data analysis used Kruskal Wallis test followed by Mann Whitney test for subacute toxicity test. Results and discussion: showed that the administration of cocoa bean extract at doses of 4500 mg/kgBB, 5500 mg/kgBB, 6500 mg/kgBB, and 7500 mg/kgBB had an effect on subacute toxicity at the time of administration to mice which caused the death of mice and affected the hepatic histopathology of mice. Conclusion: The conclusion is that cocoa bean extract at certain doses has an effect on subacute toxicity in mice.

Acute Toxicity and Allergy Test of Pegagan Leaf Extract (Centella asiatica) on Mice (Mus muscullus) Hendri Poernomo; Mochammad Taha Ma'aruf; Luh Gede Pradnyani
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 20 No. 3 (2024): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v20i3.10527

Introduction: Gotu kola (Centella asiatica) is a type of plant that is often used as a traditional medicine that has the properties to accelerate wound healing, rheumatic inflammation, asthma, hemorrhoids, dysentery, fever, and as a blood enhancer. The use of plants as medicinal raw materials is increasing, so it is necessary to prove its safety by conducting acute toxicity tests and allergy tests. Material and Method: in vivo laboratory experiment with quasi experimental method and post test-Only Control Group Design with histopathological observations on the liver of mice. Mice will be grouped into 5 groups and each group consists of 5 mice consisting of 1 control group and 4 treatment groups. The treatment group was given gotu kola leaf extract orally and observations were made for 14 days. Result and Discussion: obtained the results of acute toxicity test research, obtained LD50 value is 12.5gr/kg BW (mild toxic), on microscopic observation in the control group there was no damage to the liver, while in the treatment group there were changes in the form of liver damage. For acute toxicity, the weight of mice after being given gotu kola leaf extract was tested using Shapiro Wilk and Kruskal Wallis tests. From the analysis results, there was no significant difference. Conclusion: Gotu kola leaf extract does not cause toxic effects on mice mortality and also does not cause allergies in mice.

Effectiveness Extract Gel of Kemangi Leaves (Ocimum basilicum L.) To The Level of Fibroblast For Incision Wound Healing of Wistar RATS (Rattus norvegicus) Hendri Poernomo; Setiawan; Yuni Darma Dewi
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 21 No. 1 (2025): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v21i1.11413

Introduction: Wound healing using herbal plants is considered safer than chemical drugs. One of the plants that can be used for wound healing is basil leaves (Ocimum basilicum L.). The purpose of this study was to evaluate the effectiveness of administering basil leaf extract gel on the number of fibroblast cells in the healing process of incision wounds in Wistar rats. Research method: This study used an experimental post-test only control group design using 24 male Wistar rats. The research sample was divided into four groups, namely the negative control group (CMC-Na gel), the group given basil leaf extract gel with concentrations of 70%, 80%, and 90%. In vivo testing was carried out by making an incision wound with a length of 20mm and a subcutaneous depth on the back of the rat. The extract was given twice a day for one week, then decapitation was carried out on the 8th day. Research results: Basil extract gel with concentrations of 70%, 80%, and 90% was effective in healing incision wounds. One Way Anova test showed significant differences between the four groups (ρ<0.05). Conclusion: Basil leaf extract gel with a concentration of 80% is more effective in accelerating wound healing

Effectiveness of Cengkeh Leaf Extract Ointment (Syzygium Aromaticum L.) on The Number of Fibroblast Cells on Healing of Injection Wistar Walls (Rattus norvegicus) Poernomo, Hendri; Ma’ruf, Mochammad Taha; Mbiliyora, Paskarani
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 21 No. 2 (2025): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v21i2.12058

Introduction: An open wound is a condition where part of the body's tissue has been damaged from its normal state due to sharp or blunt trauma, changes in temperature, chemicals, explosions, electric shock, or animal bites. Clove leaves contain active ingredients such as eugenol, saponins, phenols, steroids, terpenoids, alkaloids, flavonoids, and tannins, as well as essential oils, which have the potential to be anti-inflammatory, antibacterial, and analgesic, inhibiting cell damage and accelerating the healing process in wounds. Materials and Methods: This type of research is an in vivo laboratory experiment with a posttest-only control group design and sample grouping using a Completely Randomized Design (CRD). The treatment was carried out for 5 days by applying it to the mice's backs twice a day, then on the 6th day, decapitation was carried out, taking specimens to observe the number of fibroblast cells. Results and Discussions: The research was analyzed using one-way ANOVA followed by the Least Significant Difference (LSD) test. The results of the study showed that administering cloveleaf extract ointment (Syzygium Aromaticum L.) with concentrations of 3%, 6% and 9% was effective in increasing the number of fibroblast cells in the healing process of incisional wounds on the backs of Wistar rats (Rattus norvegicus). Conclusion: Giving cloveleaf extract ointment at a concentration of 3% is more effective in increasing the number of fibroblast cells in the healing process of incisional wounds in mice compared to concentrations of 6% and 9%.

Sub-Acute Toxicity and Allergy Studies of Bidara Leaf (Ziziphus Mauritiana) Extract as A Wound Healing Material Ma'ruf, Mochammad Taha; Dewi, Putu Sulistiawati; Poernomo, Hendri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.194-201

Background: In dentistry, the wound healing process is often encountered in tooth extractions, incisions, oral and facial surgery, gingival flaps and biopsies. Post-tooth extraction wound is a medium that allows pathogenic microbes to breed and infect the wound. Wound management must be carried out as soon as possible to restore mucosal integrity so as to prevent bacterial infections penetrates the body. Bidara (Ziziphus mauritiana) is one of the plants used as traditional medicine by the community. Research on this plant as a medicinal raw material is increasing, so further research needs to be carried out to prove the safety of this medicinal plant. This study aims to determine the effects of sub-acute toxicity and allergic reactions caused after administering bidara leaf extract on the liver histopathology of mice. Methods: In vivo laboratory experimental study using a post-test only control group design with 24 mice (Mus musculus). The research sample consisted of 4 groups, namely the control group given 0.3% Na-CMC, the treatment group given bidara leaf extract at a dose of 500 mg/kg BW, 600 mg/kg BW, and 700 mg/kg BW. The treatment extract was given orally for 28 days. Results: The results of the study showed that the dose of bidara leaf extract had an effect on the liver histopathology of mice, namely at doses of 600 mg/kg BW and 700 mg/kg BW it had a toxic effect that was visible from changes in liver cell structure. Allergy tests showed that bidara leaf extract did not cause allergic reactions in mice. Conclusion: It can be concluded that bidara leaf extract (Ziziphus mauritiana) does not cause allergies and at certain doses does not have toxicity in the liver of animals study.

EDUKASI MASYARAKAT MELALUI PAMFLET SEBAGAI UPAYA PENCEGAHAN GIGI BERLUBANG DAN PENYAKIT MULUT Kadek Dwi Dessy Sapitri; Hendri Poernomo; Ni Wayan Arni Sardi
LOKATARA SARASWATI Vol 4 No 2 (2025): Lokatara Saraswati: Jurnal Pengabdian kepada Masayarakat
Publisher : Fakultas Bahasa Asing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/lokasaraswati.v4i2.12847

Besakih Village, located in Rendang District, Karangasem Regency, Bali, is a culturally rich village known for Besakih Temple, the largest and holiest temple in Bali, which also serves as a major tourist attraction. However, observations reveal that the community still lacks adequate oral health awareness. The level of knowledge about dental diseases such as cavities and periodontal disease remains low, increasing the risk of oral health problems. To address this issue, a Social Service program was held in 2025 focusing on oral health care in Besakih Village. This program provided free dental treatments including scaling, cavity fillings, and tooth extractions. In addition to treatment, oral health education was conducted, covering dental anatomy, cavity care, and prevention of periodontal disease. Informational pamphlets on oral hygiene maintenance were distributed to the community. The program targeted children and the general population to improve knowledge and awareness of oral health maintenance. It is expected that through this Social Service activity, the oral health quality of Besakih Village residents will significantly improve.

Sub-Acute Toxicity and Allergy Studies of Bidara Leaf (Ziziphus Mauritiana) Extract as A Wound Healing Material Ma'ruf, Mochammad Taha; Dewi, Putu Sulistiawati; Poernomo, Hendri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.194-201

Background: In dentistry, the wound healing process is often encountered in tooth extractions, incisions, oral and facial surgery, gingival flaps and biopsies. Post-tooth extraction wound is a medium that allows pathogenic microbes to breed and infect the wound. Wound management must be carried out as soon as possible to restore mucosal integrity so as to prevent bacterial infections penetrates the body. Bidara (Ziziphus mauritiana) is one of the plants used as traditional medicine by the community. Research on this plant as a medicinal raw material is increasing, so further research needs to be carried out to prove the safety of this medicinal plant. This study aims to determine the effects of sub-acute toxicity and allergic reactions caused after administering bidara leaf extract on the liver histopathology of mice. Methods: In vivo laboratory experimental study using a post-test only control group design with 24 mice (Mus musculus). The research sample consisted of 4 groups, namely the control group given 0.3% Na-CMC, the treatment group given bidara leaf extract at a dose of 500 mg/kg BW, 600 mg/kg BW, and 700 mg/kg BW. The treatment extract was given orally for 28 days. Results: The results of the study showed that the dose of bidara leaf extract had an effect on the liver histopathology of mice, namely at doses of 600 mg/kg BW and 700 mg/kg BW it had a toxic effect that was visible from changes in liver cell structure. Allergy tests showed that bidara leaf extract did not cause allergic reactions in mice. Conclusion: It can be concluded that bidara leaf extract (Ziziphus mauritiana) does not cause allergies and at certain doses does not have toxicity in the liver of animals study.

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