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All Journal INDONESIAN JOURNAL OF PHARMACY JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Indonesian Journal of Life Sciences

Leonny Yulita Hartiadi

Indonesia International Institute For Life Sciences

Author-ID : 553059

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Environmental Science Medicine & Pharmacology Public Health Social Sciences

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Articles

HEPATOPROTECTIVE ACTIVITY OF SAPONIN FRACTION OF OYONG SEED FLESH AND ITS COMBINATION AGAINST CCl4-INDUCED CHRONIC LIVER DAMAGE IN MALE WISTAR RAT Rachmawati, Heni; Y. Hartiadi, R. Leonny; Fidrianny, Irda; Adnyana, I Ketut
Indonesian Journal of Pharmacy Vol 24 No 3, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Saponin fraction of seed flesh of Oyong (Luffaacutangula [L.]Roxb) has been investigated to have a hepatoprotective activity in rats with fibrotic chronic liver damage. This research was conducted to evaluate whether saponin fraction of Oyongseed flesh has a hepatoprotective activity in CCl4-induced acute liver damage. Hepatoprotective activity was determined by measuring the activity of liver enzymes (SGOT, SGPT, LDH), total nitrite/nitrate level, liver index and liver histology. Saponin fraction of Oyong flesh seeds 10mg/kg BW and meniran extract 400mg/kg BW alone showed hepatoprotective activity. Administration of saponin fraction 10mg/kb BB decreased SGPT and LDH significantly over untreated group. Group given meniran extract at dose of 200mg/kg BW showed decreased on LDH, while at dose of 400mg/kg BW decreased SGPT, SGOT, and LDH significantly. Hystological observation revealed any improvement in liver morphology especially after treated with saponin fraction 10mg/kg BWand meniran extract at dose of 400mg/kg BW. However, all groups treated with combination of saponin and meniran did not show improvement both at biochemical parameter and liver histology. In conclusion, saponin extract with dose of 10mg/kg BW and meniran extract at dose of 400mg/kg BW showed hepatoprotector activity. In contrast, combination of both did not show any hepatoprotective effect and it was suspected that they have antagonist effects.Key words:hepatoprotective, CCl4-induced liver fibrosis, Luffaacutangula, Phyllanthusniruri

Penyuluhan dan Workshop Obat Nyamuk Sintetis dan Alami Sebagai Tindakan Pencegahan DBD Agnes Anania Triavika Sahamastuti; Elsabda Siratan; Theodore Ebenezer Leonard; Gisella Edny Tjugianto; Leonny Yulita Hartiadi; Istiqomah Agusta
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 3 NOMOR 2 SEPTEMBER 2019 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Selama bulan Januari-Februari 2016, Jakarta Timur menduduki peringkat kedua dalam jumlah penderita DBD. Hal ini diperkirakan karena kurangnya partisipasi masyarakat dalam pemberantasan sarang nyamuk dan menjaga sanitasi. Hingga saat ini, masyarakat umum menggunakan obat pengusir nyamuk untuk menghindari gigitan nyamuk. Namun penggunaan pengusir nyamuk sintetis dengan cara yang salah dan dalam jangka waktu lama dapat berdampak buruk terhadap kesehatan. Kegiatan Pengabdian Masyarakat ini bertujuan untuk meningkatkan pengetahuan masyarakat mengenai bahaya penggunaan obat pengusir nyamuk sintetis dan pemberian solusi melalui bahan alamiah berkhasiat mengusir nyamuk. Kegiatan ini dilakukan di wilayah RW 11, Pulomas Barat, DKI Jakarta dengan melibatkan kelompok ibu PKK dan Jumantik (Jumat Bebas Jentik) dan dihadiri oleh 37 orang partisipan. Hasil evaluasi menunjukkan bahwa pengetahuan para partisipan mengenai bahaya obat nyamuk sintetis beserta cara pembuatan obat nyamuk alami meningkat setelah penyuluhan. Data mengenai tingkat penggunaan obat pengusir nyamuk di daerah warga juga berhasil diperoleh. Para partisipan dilatih membuat lotion pengusir nyamuk dari bahan alami diakhir kegiatan. Kegiatan ini diharapkan dapat meningkatkan kesadaran masyarakat untuk berpartisipasi aktif dalam menurunkan angka kejadian penyakit DBD dengan memanfaatkan bahan-bahan alami yang terjangkau dan mudah diperoleh.

Comparison of Antimicrobial Activity between Ethanolic Extract and Essential Oil of Ginger (Zingiber officinale) against Food-borne Bacteria Foustine, Shania; Andre, Andre; Setiadi, Bastian; Hartiadi, Leonny Yulita; Sahamastuti, Agnes Anania Triavika
Indonesian Journal of Life Sciences 2019: IJLS Vol 01 No .02
Publisher : Indonesia International Institute for Life Sciences

Background: Ginger (Zingiber officinale) is one of the most well-known spices with antimicrobial activity. However, different extraction methods of ginger will result in different antimicrobial properties due to the various substances extracted. This study aimed to compare antimicrobial activity between ethanolic extract and essential oil of ginger against food-borne bacteria grown in 2.1% Mueller Hinton agar. Methods: Fresh ginger rhizomes were extracted either by using vapor distillation method or maceration using ethanol to obtain ginger essential oils (GEO) and ginger ethanolic extract (GEE), respectively. Ethanolic extract and essential oil of ginger at different concentrations were then tested for their antimicrobial activity using disk diffusion method against Escherichia coli, Bacillus subtilis, Salmonella typhi, and Staphylococcus aureus. Tetracycline was also used as a standard of antibacterial agent. Results: Ginger essential oil in 25%, 50%, and 100% showed significant growth inhibition of four types of bacteria compared to ginger ethanolic extract. This antimicrobial effect of ginger essential oil was shown to be dose-dependent. However, it has been demonstrated that ginger ethanolic extract 50% and 100% has a stronger antimicrobial effect against B.subtilis. Conclusion: Ginger essential oil and ethanolic extract showed different degree of antibacterial activity against food-borne bacteria due to compound contained within respective extracts, with a higher degree of activity found in ginger essential oil. These may show that different ginger extract may have different antibacterial activity.

Combination of Piper betel Leaves and Areca catechu Nuts Ethanolic Extract Effects on In Vitro Antimicrobial Test against Aerobic Mouth Microbiota Christanto, Raymond; Andre, Andre; Foustine, Shania; Hartiadi, Leonny Yulita
Indonesian Journal of Life Sciences 2019: IJLS Vol 01 No .02
Publisher : Indonesia International Institute for Life Sciences

Introduction: Oral health awareness in Indonesia is still low with more and more oral and/or dental infection becomes more prevalent such as gingivitis, endodontitis, and pericoronitis. These infections could be caused by mouth microflora such as lactobacilli, Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, and Streptococcus sobrinus. As time goes by, these bacteria could also become more problematic as antibacterial resistance emerges. Betel nut and betel leaf are well known traditional herbs that is often used in combination for "nyirih"; an Indonesian tradition to chew both plants often after meal. It is believed that these plants treat bad breath and also increases oral health as it could inhibit bacterial growth. Method: Betel nut and betel leaf were dried, powdered and macerated using 96% ethanol. The extract was tested on mouth microflora of volunteers grown in BHI using agar using disk diffusion test and modified E-test strip. Results: The betel leaves extract showed antibacterial activity in most of concentration used (1.25%, 2.5%, 5%, 10%) while betel nuts only gave antimicrobial effect in 10% concentration. The E-test results also showed the synergistic action between Piper betel leaves and Areca catechu nuts with some giving significant difference compared to the single treatment (P<0.05). Conclusion: Piper betel leaves gives antimicrobial effect with MIC value of 1.25% while Areca catechu have MIC value of 10%. Furthermore, combination of both resulted in synergistic effect as indicated with larger diameter of inhibition compared to the single treatments.

Synergistic Antibacterial Activities of Ginger and Lemongrass Essential Oils as an Alternative Prevention to Food-Borne Disease Sahamastuti, Agnes Anania Triavika; Andre, Andre; Foustine, Shania; Sumarpo, Anton; Hartiadi, Leonny Yulita
Indonesian Journal of Life Sciences 2019: IJLS Vol 01 No .02
Publisher : Indonesia International Institute for Life Sciences

Background: Lemongrass and ginger are traditional food ingredients in Asian countries, including Indonesia, Thailand, India, and Malaysia. Although their single essential oil has been assessed for its antibacterial activities, no report has been done for their combination. Material and methods: Our study evaluated single and combination of these herbs for their antibacterial properties against food-borne bacteria E. coli (NEB® catalog No. C2989K), B. subtilis (ATCC 6633), S. typhi (ATCC 14028) and S. aureus (InaCC B4). Essential oil of lemongrass and ginger were obtained by steam distillation and their antimicrobial were evaluated using disk diffusion assay with chloramphenicol as the standard antibiotic. Synergistic activity was assessed using the combination of materials at two or four-fold dilution from their respected MIC value. Results: We confirmed that single lemongrass and ginger essential oils inhibited the bacteria growth with MIC value of about 1-5% and 2.5-5%, respectively. Moreover, their synergism activities were observed when both were mixed at two-fold dilution from their respective MIC. Conclusion: We conclude that the combination of the ginger essential oils and lemongrass essential oils may have potential as a natural preservative to prevent food-borne diseases.

Cytotoxicity Evaluation of Bacteria Extract Isolated from Indonesia Sheep Manures and Fungal Fruiting Body Hartiadi, Leonny Yulita; Winiati, Immaculata Titis; -, samirah -; Gomez, Cristina
Indonesian Journal of Life Sciences 2020: IJLS Vol 02 No .01
Publisher : Indonesia International Institute for Life Sciences

Introduction: Cancer is one of the leading cause of death worldwide. Chemotherapy remains the most widely used approach to treat cancer. However, due to the lack of specificity, drug resistance, and undesirable side effects, the discovery of novel anticancer drugs is still a priority in cancer research. Bacteria are one of the major contributors for anticancer compounds as bacteria are able to produce secondary metabolites which may have cytotoxic activity. Method: A literature study was conducted to identify the isolates in i3L-USAID bacteria database that were potential candidates for novel anticancer drugs. The selected bacteria were cultured and their secondary metabolites were extracted. Cytotoxic activity of the extracts was assessed through morphological observation and MTT assay on HeLa and 3T3 fibroblast cell lines. Results: Aneurinibacillus sp. and Ochrobactrum sp. were selected. Ochrobactrum sp. extract significantly suppressed the growth of HeLa cells at 1000 µg/ml (p < 0.05) while it did not have any effect on 3T3 cells. Despite able to suppress the HeLa cell proliferation, Aneurinibacillus sp. extract significantly enhanced the growth of 3T3 fibroblast at the 1000 µg/ml (p < 0.05). The cytotoxicity activity was corroborated by cell death observed on HeLa cells treated with high concentrations of the extracts Conclusion: Secondary metabolites from Aneurinibacillus sp. and Ochrobactrum sp. could only exert cytotoxic effect at high concentration which is unfavourable for anticancer compounds. Although the extracts resulted in a reduction of HeLa cells proliferation, the extracts did not inhibit proliferation of 3T3, suggesting selectivity for non-tumorigenic over tumorigenic cells.

Co-Authors Agnes Anania Triavika Sahamastuti Andre Andre, Andre Anton Sumarpo Christanto, Raymond Elsabda Siratan Foustine, Shania Gisella Edny Tjugianto Gomez, Cristina HENI RACHMAWATI I Ketut Adnyana Irda Fidrianny Istiqomah Agusta Samirah - - Setiadi, Bastian Theodore Ebenezer Leonard Winiati, Immaculata Titis

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