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All Journal Jurnal Ilmu Dasar Indonesian Journal of Biotechnology and Biodiversity Journal of Tropical Crop Science JURNAL PANGAN Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia
N. Sri Hartati, N. Sri
Pusat Penelitian Bioteknologi-LIPI, Jln. Raya Bogor Km 46 Cibinong 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Materials Science & Nanotechnology Mathematics Social Sciences Other

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Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures Supatmi, Supatmi; Rahman, Nurhamidar; Hartati, N. Sri
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

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Abstract

Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature.
Identification of Differentially Expressed cDNA in Cassava under Drought Stress Using cDNA-RAPD Approach Hartati, N. Sri; Supatmi, Supatmi; Aryaningrum, Pramesti Dwi; Sudarmonowati, Enny
Annales Bogorienses Vol. 17 No. 1 (2013): Annales Bogorienses
Publisher : BRIN

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Cassave is an important carbohydrate
OPTIMIZATION OF Agrobacterium-MEDIATED GENETIC TRANSFORMATION OF oshox4 GENE IN SATOIMO TARO (Colocasia esculenta var. antiquorum) Hartati, N. Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1791

Abstract

Satoimo (Colocasia esculenta var. antiquorum) can be considered as an alternative food to support food diversification. The aim of this research was to obtain an optimum condition of the genetic transformation method of Satoimo taro through Agrobacterium-mediated genetic transformation using binary vector harboring gene construct of oshox4 and hpt gene as a selectable marker to produce transgenic plants. Plant materials that were used in this study were meristem, root, leaf, petiole, and basal stem. Transformation procedures were carried out using three different co-cultivation periods (1, 2, and 3 days) and different optical densities of A. tumefaciens cells (0,5 and 0,7). Furthermore, callus induction was performed on MS medium containing 2.4-D, cefotaxime, hygromycin and then regenerated on MS medium containing thidiazuron and hygromycin. The putative transgenic plantlets were selected on MS medium containing hygromycin and analyzed using PCR. The results showed that putative transgenic plants derived from the basal stem could survive on a selection medium containing hygromycin and based on PCR analysis some of them contained the oshox4 and hpt genes.
Co-Authors Ahmad Fathoni Anjarsari, Nurul Aeni Aryaningrum, Pramesti Dwi Dwi Hilda Putri Enny Sudarmonowati Enung Sri Mulyaningsih Hani Fitriani, Hani Hartati Hartati Hartati, H. Mayasti, Nur Kartika Indah Prita Sari Dewi Rahman, Nurhaidar Rahman, Nurhamidar Ramadanti, Nur Ayu Sintho Wahyuning Ardie Siti Kurniawati Sudarsono Supatmi Supatmi Ulfa, Umi Tahniah