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All Journal JURNAL HUTAN LESTARI Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Nusantara Medical Science Journal
Nita Mariana
Division of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Agriculture, Biological Sciences & Forestry Immunology & microbiology Materials Science & Nanotechnology Neuroscience

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Journal : Nusantara Medical Science Journal

 1 
Effect of Stromal Vascular Fraction and Platelet-Rich Plasma Combination on Polymorphonuclear Cells in Wistar Rats Anal Trauma Model Erwin Chandra; Sulmiati Sulmiati; Nita Mariana; Fonny Josh; Sachraswaty Rachman; Firdaus Hamid
Nusantara Medical Science Journal Volume 7 Issue 2, July - December 2022
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v7i2.20846

Abstract

Introduction: By relying on polymorphonuclear (PMN) cells, stromal vascular fraction (SVF) and platelet-rich plasma (PRP) contribute significantly in the healing process. Through anal trauma model, this study examined the effect of SVF and PRP combination on PMN counts in vivo. Method: Three groups of twenty-eight eligible Wistar rats anal trauma model randomly assigned to receive SVF and PRP combination (Group A), normal saline (Group B), or healthy controls (Group C). On days 1, 7, and 14, we examined at PMN cell counts in Groups A and B. Group C were sacrificed early to acquire baseline data on PMN cells. Results:  On the first day, Group A had more PMN cells than Group B, but this difference faded by days 7 and 14. On day 1, the test revealed a significant increase in PMN cells (p=0.002). Conclusion:  Combining SVF and PRP can improve PMN cells' acute phase of anal damage repair in vivo
Immunohistochemical Diagnostic value of Calretinin in Hirschprung Disease Azis, Zainab; Cangara, M. Husni; Zainuddin, Andi Alfian; Wahid, Syarifuddin; Mariana, Nita; Miskad, Upik A.
Nusantara Medical Science Journal Volume 8 Issue 2, July - December 2023
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.vi.20000

Abstract

Introduction: Hirschsprung's disease (HD) is characterized by the absence of ganglion cells in Meissner's plexus and Auerbach's plexus which used to be assessed by histopathological examination using Hematoxylin and Eosin (H&E).  In some cases is difficult to assess ganglion cells, thus requiring additional examination in the form of calretinin immunohistochemistry to detect the presence of ganglion. Methods: The 59 samples of suspected HD  were analyzed by H&E staining followed by calretinin immunohistochemical staining. This study aimed to assess the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of calretinin in HD diagnostics. Results:  The immunohistochemistry of calretinin showed a good diagnostic value in detecting aganglionosis in patients with suspected HD with a sensitivity value = 97.7%; Specificity = 100%; Positive Presumptive Value (PPV) = 100%; Negative Presumptive Value (NPV) = 93.7%; Accuracy= 98.3%. Thus, calretinin immunohistochemistry can be relied upon as an additional method in the diagnosis of HD
Co-Authors . Ahmadwirawan . Prihantono . Sulmiati Andi Alfian Zainuddin Andi Alfian Zainuddin Azis, Zainab Cangara, M. Husni Erwin Chandra Farid Nurmantu Firdaus Hamid Fonny Josh Harsali F. Lampus Iswan Dewantara Reinaldo Sunggiardi Sachraswaty Rachman Sulmiati Sulmiati Syarifuddin Wahid Tommy R. Habar Upik A. Miskad, Upik A. Yeni Mariani